Q6KZR2 (PURL_PICTO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase 2
EC=6.3.5.3

Alternative name(s):
Phosphoribosylformylglycinamidine synthase II
Short name=FGAM synthase II

Gene names

Name:	purL
Ordered Locus Names:	PTO1205

Organism

Picrophilus torridus (strain ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828) [Complete proteome] [HAMAP]

Taxonomic identifier

263820 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermoplasmata › Thermoplasmatales › Picrophilaceae › Picrophilus

Protein attributes

Sequence length	741 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00420
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00420
Subunit structure	Heterodimer of two subunits, PurQ and PurL.
Subcellular location	Cytoplasm By similarity HAMAP MF_00420.
Sequence similarities	Belongs to the FGAMS family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 741	741	Phosphoribosylformylglycinamidine synthase 2 HAMAP MF_00420		PRO_0000100518
Regions
	Nucleotide binding	113 – 124	12	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q6KZR2 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: F9527AA0502F51D8	FASTA	741	82,190
	10 20 30 40 50 60 MDIINCSDND LDLIGKSLAL SHDEMLLIKK YFTEIKRNPS DVELQAIAQS WSEHSCYKSS 70 80 90 100 110 120 KFYLKKYLSN LRNERTILAM EDDAGVVKFN DDYVYVVKME SHNHPSAVEP YGGAATGVGG 130 140 150 160 170 180 IIRDVLCMGA QPVALVDSLY FGDPDNKSGF LSERFIINGV VSGIRDYGNR LGIPNVAGSI 190 200 210 220 230 240 YFHEGYNTSP IVNAGCIGIS RKDKIVRSRV QKARDILILC GGRTGRDGIH GVNFASRVLD 250 260 270 280 290 300 LKNGENRNAV QLGNPIVEEP LIHAILELND LGLITGMKDL GGGGLSSAVT EMLYAGNLGG 310 320 330 340 350 360 TINLDSVLLK DDNMLPWEIW ISESQERMLI SSDERNLPQI KDVLDKWNIE FSVIGRAEEK 370 380 390 400 410 420 KNLEIYYKNE KVFDLPLEFI SKTPVYQRPY KKPRNRCKSE PFRDDDINKS IISLISSINV 430 440 450 460 470 480 CSRAPVIRQY DHTVRGATIV RPLTGLPNNE THSDAAVIKP VDDSFAGIAV TSGSKPMLCT 490 500 510 520 530 540 IDPYGGALES LIEAYKNIIV TGAEPDAIVD SLNFGNPENE ETMYSFVETL KAIRDFTLKF 550 560 570 580 590 600 KLQLVSGNVS FYNKNVSDIM PTPNIMMTGI IDDVRKAITT EFKNKNSLIY LIGSINGSLA 610 620 630 640 650 660 GTVYSKLKDI KCYDYHNSNI NDLCNVYNII KENKEKILAA HDVSDGGIIA ALIEMSFGKN 670 680 690 700 710 720 IGFNVNLKDI KMNLENKLFS EHGTAIVIEV PLESEIVFNN LGIKLGYTCD DITVMDGENM 730 740 VFNARISELK NLWDSGLGKY L

References

[1]

"Genome sequence of Picrophilus torridus and its implications for life around pH 0." Fuetterer O., Angelov A., Liesegang H., Gottschalk G., Schleper C., Schepers B., Dock C., Antranikian G., Liebl W. Proc. Natl. Acad. Sci. U.S.A. 101:9091-9096(2004) [PubMed: 15184674] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017261 Genomic DNA. Translation: AAT43790.1.
RefSeq	YP_023983.1. NC_005877.1.
3D structure databases
ProteinModelPortal	Q6KZR2.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2844297.
GenomeReviews	Gene locus PTO1205 in contig AE017261_GR.
KEGG	pto:PTO1205.
NMPDR	fig|263820.1.peg.1205.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG311214.
OMA	YGNSFGV.
PhylomeDB	Q6KZR2.
ProtClustDB	PRK01213.
Enzyme and pathway databases
BioCyc	PTOR263820:PTO1205-MONOMER.
Family and domain databases
HAMAP	MF_00420. PurL_2. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR010074. PRibForGlyAmidine_synth_II. IPR016188. PurM_N-like. [Graphical view]
KO	K01952.
Pfam	PF00586. AIRS. 2 hits. PF02769. AIRS_C. 2 hits. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 2 hits. SSF55326. PurM_N-like. 2 hits.
TIGRFAMs	TIGR01736. FGAM_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PURL_PICTO

Accession

Primary (citable) accession number: Q6KZR2

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 21, 2004
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program