Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Enolase

Gene

eno

Organism
Picrophilus torridus (strain ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis.UniRule annotation

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

The covalent binding to the substrate causes inactivation of the enzyme, and possibly serves as a signal for the export of the protein.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (gap)
  2. Phosphoglycerate kinase (pgk)
  3. 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (apgM)
  4. Enolase (eno)
  5. Pyruvate kinase (PTO0336)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei146SubstrateUniRule annotation1
Binding sitei155SubstrateUniRule annotation1
Active sitei197Proton donorUniRule annotation1
Metal bindingi233MagnesiumUniRule annotation1
Metal bindingi274MagnesiumUniRule annotation1
Binding sitei274SubstrateUniRule annotation1
Metal bindingi301MagnesiumUniRule annotation1
Binding sitei301SubstrateUniRule annotation1
Active sitei326Proton acceptorUniRule annotation1
Binding sitei326Substrate (covalent); in inhibited formUniRule annotation1
Binding sitei377SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processGlycolysis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciPTOR263820:G1G0U-1315-MONOMER
UniPathwayiUPA00109; UER00187

Names & Taxonomyi

Protein namesi
Recommended name:
EnolaseUniRule annotation (EC:4.2.1.11UniRule annotation)
Alternative name(s):
2-phospho-D-glycerate hydro-lyaseUniRule annotation
2-phosphoglycerate dehydrataseUniRule annotation
Gene namesi
Name:enoUniRule annotation
Ordered Locus Names:PTO1234
OrganismiPicrophilus torridus (strain ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828)
Taxonomic identifieri263820 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermoplasmataThermoplasmatalesPicrophilaceaePicrophilus
Proteomesi
  • UP000000438 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation
  • Secreted UniRule annotation
  • Cell surface UniRule annotation
  • Note: Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface.UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002808831 – 400EnolaseAdd BLAST400

Interactioni

Protein-protein interaction databases

STRINGi263820.PTO1234

Structurei

3D structure databases

ProteinModelPortaliQ6KZN3
SMRiQ6KZN3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni353 – 356Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the enolase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01169 Archaea
COG0148 LUCA
HOGENOMiHOG000072173
KOiK01689
OMAiQEFLVVP
OrthoDBiPOG093Z03D9

Family and domain databases

CDDicd03313 enolase, 1 hit
Gene3Di3.20.20.120, 1 hit
3.30.390.10, 1 hit
HAMAPiMF_00318 Enolase, 1 hit
InterProiView protein in InterPro
IPR000941 Enolase
IPR036849 Enolase-like_C_sf
IPR029017 Enolase-like_N
IPR020810 Enolase_C
IPR020809 Enolase_CS
IPR020811 Enolase_N
PANTHERiPTHR11902 PTHR11902, 1 hit
PfamiView protein in Pfam
PF00113 Enolase_C, 1 hit
PF03952 Enolase_N, 1 hit
PIRSFiPIRSF001400 Enolase, 1 hit
PRINTSiPR00148 ENOLASE
SFLDiSFLDG00178 enolase, 1 hit
SMARTiView protein in SMART
SM01192 Enolase_C, 1 hit
SM01193 Enolase_N, 1 hit
SUPFAMiSSF51604 SSF51604, 1 hit
PROSITEiView protein in PROSITE
PS00164 ENOLASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q6KZN3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNFEIKDTNI RKIFDSRGNF SAEVTVILEN ASGTASAPAG ASTGKTEVIA
60 70 80 90 100
YPDNNIDNGI DFFYRHVKKA LKGFNSINQE GLDRMLHEID GTDNFSNLGG
110 120 130 140 150
NIATAISIAN AKAVSNALGI PMYRYVGGIN YSMPRPIGNV IGGGKHSKNG
160 170 180 190 200
TTIQEFLVSA QGKTVLDSIY YNILIHRRIG EILSGMFKNQ SIGLGDEKAW
210 220 230 240 250
TCDISDEDAI EIIKNASKDI SSEYKIKVLN GVDFAADSFY DGNYYIYKNR
260 270 280 290 300
KLTRDQQIDY AISISRDHGF YYIEDPLNDQ DFDGFSEITS KVGDKSLIVG
310 320 330 340 350
DDLYTTNPER IKKGIEKRST NGVLIKVNQI GTLSDTARSV RIATEHGLKT
360 370 380 390 400
VVSHRSGETT DDFIAHLAVA FGSPLIKTGT IGGERLAKLN ELIRIEEELI
Length:400
Mass (Da):43,961
Last modified:July 5, 2004 - v1
Checksum:iAC3F073E78ADD4DA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017261 Genomic DNA Translation: AAT43819.1
RefSeqiWP_011178035.1, NC_005877.1

Genome annotation databases

EnsemblBacteriaiAAT43819; AAT43819; PTO1234
GeneIDi2844318
KEGGipto:PTO1234
PATRICifig|263820.9.peg.1282

Similar proteinsi

Entry informationi

Entry nameiENO_PICTO
AccessioniPrimary (citable) accession number: Q6KZN3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: July 5, 2004
Last modified: March 28, 2018
This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health