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Protein

Glutamate formimidoyltransferase

Gene

PTO1242

Organism
Picrophilus torridus (strain ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of the formyl group from N-formylglutamate to tetrahydrofolate (THF) to yield 5-formyltetrahydrofolate (5-CHO-THF) and glutamate (Glu). The triglutamate form of 5-CHO-THF (5-CHO-THF-Glu3) can also be used as substrate. It can also catalyzes the transfer of the formimino group from N-formiminoglutamate to tetrahydrofolate (THF) to yield 5-formiminotetrahydrofolate (5-NH=CH-THF) and glutamate (Glu). It can replace YgfA to catalyzes the irreversible ATP-dependent transformation of 5-CHO-THF to form 5,10-methenyltetrahydrofolate (5,10-CH=THF).1 Publication

Catalytic activityi

5-formyltetrahydrofolate + L-glutamate = tetrahydrofolate + N-formyl-L-glutamate.1 Publication
5-formimidoyltetrahydrofolate + L-glutamate = tetrahydrofolate + N-formimidoyl-L-glutamate.1 Publication
ATP + 5-formyltetrahydrofolate = ADP + phosphate + 5,10-methenyltetrahydrofolate.1 Publication

Kineticsi

Kcat is 1.16 sec(-1) for formyltransferase with 5-CHO-THF or 5-CHO-THF-Glu3 as substrates (at pH 7.3 and 30 degrees Celsius).

  1. KM=0.4 µM for 5-CHO-THF (at pH 7.3 and 30 degrees Celsius)1 Publication
  2. KM=0.7 µM for 5-CHO-THF-Glu3 (at pH 7.3 and 30 degrees Celsius)1 Publication
  3. KM=34 µM for Glu (at pH 7.3 and 30 degrees Celsius)1 Publication

    Pathwayi: L-histidine degradation into L-glutamate

    This protein is involved in step 1 of the subpathway that synthesizes L-glutamate from N-formimidoyl-L-glutamate (transferase route).
    Proteins known to be involved in this subpathway in this organism are:
    1. Glutamate formimidoyltransferase (PTO1242)
    This subpathway is part of the pathway L-histidine degradation into L-glutamate, which is itself part of Amino-acid degradation.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate from N-formimidoyl-L-glutamate (transferase route), the pathway L-histidine degradation into L-glutamate and in Amino-acid degradation.

    Pathwayi: tetrahydrofolate interconversion

    This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.
    View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei86 – 861For formimidoyltransferase activityBy similarity

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Ligase, Transferase

    Keywords - Biological processi

    Histidine metabolism

    Keywords - Ligandi

    Folate-binding

    Enzyme and pathway databases

    BioCyciPTOR263820:GHA3-1289-MONOMER.
    UniPathwayiUPA00193.
    UPA00379; UER00555.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamate formimidoyltransferase (EC:2.1.2.5)
    Alternative name(s):
    5-formyltetrahydrofolate cyclo-ligase (EC:6.3.3.2)
    Glutamate formiminotransferase
    Glutamate formyltransferase
    Gene namesi
    Ordered Locus Names:PTO1242
    OrganismiPicrophilus torridus (strain ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828)
    Taxonomic identifieri263820 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermoplasmataThermoplasmatalesPicrophilaceaePicrophilus
    Proteomesi
    • UP000000438 Componenti: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 301301Glutamate formimidoyltransferasePRO_0000430019Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi263820.PTO1242.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6KZM5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni168 – 1769Folate bindingSequence analysis

    Sequence similaritiesi

    Belongs to the formiminotransferase family.Curated

    Phylogenomic databases

    eggNOGiarCOG05394. Archaea.
    COG3643. LUCA.
    HOGENOMiHOG000235616.
    KOiK00603.
    OMAiMEECIEI.

    Family and domain databases

    Gene3Di3.30.70.670. 1 hit.
    3.30.990.10. 1 hit.
    InterProiIPR013802. Formiminotransferase_C.
    IPR004227. Formiminotransferase_cat.
    IPR012886. Formiminotransferase_N.
    IPR022384. FormiminoTrfase_subdom.
    [Graphical view]
    PfamiPF02971. FTCD. 1 hit.
    PF07837. FTCD_N. 1 hit.
    [Graphical view]
    SMARTiSM01221. FTCD. 1 hit.
    SM01222. FTCD_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF55116. SSF55116. 2 hits.
    TIGRFAMsiTIGR02024. FtcD. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q6KZM5-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MPDKEVKIVE AVPNFSEGRD ISKIEKIIDS IKNIEGVKIL DLNVDPQHNR
    60 70 80 90 100
    SVITFTCGIE RIIEAGISMI KTAASLIDME KHSGLHPRFG ATDVFPIIPI
    110 120 130 140 150
    TASMDDCIIA SRNLGRLVGS ELNIPVYMYS ESAMVPERRN LENIRNKNVQ
    160 170 180 190 200
    YEELKELIKT DKYRPDFGPD SLGSAGAVII GARPALIAYN IYISTDDIKI
    210 220 230 240 250
    GRRIASALRG RDGGLNTLKT LAFYIEARKM VQISMNITDY KKTSIYKIFE
    260 270 280 290 300
    LLSIECRRYN VYPVESELIG LMPMDALIDV FNYYMKSNIT RDKILEYKIF

    T
    Length:301
    Mass (Da):34,042
    Last modified:July 5, 2004 - v1
    Checksum:i0818B6088AEC299D
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE017261 Genomic DNA. Translation: AAT43827.1.
    RefSeqiWP_011178043.1. NC_005877.1.

    Genome annotation databases

    EnsemblBacteriaiAAT43827; AAT43827; PTO1242.
    GeneIDi2844665.
    KEGGipto:PTO1242.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE017261 Genomic DNA. Translation: AAT43827.1.
    RefSeqiWP_011178043.1. NC_005877.1.

    3D structure databases

    ProteinModelPortaliQ6KZM5.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi263820.PTO1242.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAT43827; AAT43827; PTO1242.
    GeneIDi2844665.
    KEGGipto:PTO1242.

    Phylogenomic databases

    eggNOGiarCOG05394. Archaea.
    COG3643. LUCA.
    HOGENOMiHOG000235616.
    KOiK00603.
    OMAiMEECIEI.

    Enzyme and pathway databases

    UniPathwayiUPA00193.
    UPA00379; UER00555.
    BioCyciPTOR263820:GHA3-1289-MONOMER.

    Family and domain databases

    Gene3Di3.30.70.670. 1 hit.
    3.30.990.10. 1 hit.
    InterProiIPR013802. Formiminotransferase_C.
    IPR004227. Formiminotransferase_cat.
    IPR012886. Formiminotransferase_N.
    IPR022384. FormiminoTrfase_subdom.
    [Graphical view]
    PfamiPF02971. FTCD. 1 hit.
    PF07837. FTCD_N. 1 hit.
    [Graphical view]
    SMARTiSM01221. FTCD. 1 hit.
    SM01222. FTCD_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF55116. SSF55116. 2 hits.
    TIGRFAMsiTIGR02024. FtcD. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiGLFT_PICTO
    AccessioniPrimary (citable) accession number: Q6KZM5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 3, 2014
    Last sequence update: July 5, 2004
    Last modified: September 7, 2016
    This is version 61 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.