Q6KZB3 (IPYR_PICTO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Inorganic pyrophosphatase
EC=3.6.1.1

Alternative name(s):
Pyrophosphate phospho-hydrolase
Short name=PPase

Gene names

Name:	ppa
Ordered Locus Names:	PTO1354

Organism

Picrophilus torridus (strain ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828) [Complete proteome] [HAMAP]

Taxonomic identifier

263820 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermoplasmata › Thermoplasmatales › Picrophilaceae › Picrophilus

Protein attributes

Sequence length	177 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Diphosphate + H2O = 2 phosphate. HAMAP MF_00209
Cofactor	Binds 4 magnesium ions per subunit. Other metal ions can support activity, but at a lower rate. Two magnesium ions are required for the activation of the enzyme and are present before substrate binds, two additional magnesium ions form complexes with substrate and product By similarity.
Subcellular location	Cytoplasm By similarity HAMAP MF_00209.
Sequence similarities	Belongs to the PPase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphate-containing compound metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	inorganic diphosphatase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 177	177	Inorganic pyrophosphatase HAMAP MF_00209		PRO_0000137554
Sites
	Metal binding	70	1	Magnesium 1 By similarity
	Metal binding	75	1	Magnesium 1 By similarity
	Metal binding	75	1	Magnesium 2 By similarity
	Metal binding	107	1	Magnesium 1 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6KZB3 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 4BB1FC5834273006	FASTA	177	20,498
	10 20 30 40 50 60 MEKNMSYWHQ VPPGPNPPDE VYVVVEIPKG ERNKYEIAKE FPGIKLDRII YSSYVYPLEY 70 80 90 100 110 120 GLIPQTYYSD GDPIDAMVFM SQSTYPGVIL RAKPVGMMNM VDSGDVDNKI ICVCLDDPVY 130 140 150 160 170 SKINNYREIP EHVLKETENF FETYKKLQNK EVKVTGWEGP DKAKQEIKKA IEDYKKL

References

[1]

"Genome sequence of Picrophilus torridus and its implications for life around pH 0." Fuetterer O., Angelov A., Liesegang H., Gottschalk G., Schleper C., Schepers B., Dock C., Antranikian G., Liebl W. Proc. Natl. Acad. Sci. U.S.A. 101:9091-9096(2004) [PubMed: 15184674] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017261 Genomic DNA. Translation: AAT43939.1.
RefSeq	YP_024132.1. NC_005877.1.
3D structure databases
ProteinModelPortal	Q6KZB3.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2845201.
GenomeReviews	Gene locus PTO1354 in contig AE017261_GR.
KEGG	pto:PTO1354.
NMPDR	fig|263820.1.peg.1354.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG529150.
OMA	PNDFNVI.
PhylomeDB	Q6KZB3.
ProtClustDB	CLSK227943.
Enzyme and pathway databases
BioCyc	PTOR263820:PTO1354-MONOMER.
Family and domain databases
HAMAP	MF_00209. Inorganic_PPase. [Tree]
InterPro	IPR008162. Pyrophosphatase. [Graphical view]
Gene3D	G3DSA:3.90.80.10. Pyrophosphatase. 1 hit.
KO	K01507.
PANTHER	PTHR10286. Pyrophosphatase. 1 hit.
Pfam	PF00719. Pyrophosphatase. 1 hit. [Graphical view]
SUPFAM	SSF50324. Pyrophosphatase. 1 hit.
PROSITE	PS00387. PPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IPYR_PICTO

Accession

Primary (citable) accession number: Q6KZB3

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	July 5, 2004
Last modified:	December 14, 2011
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program