Reviewed, UniProtKB/Swiss-Prot Q6KNA9 (METE_BACAN)

Names and origin

Protein names

Recommended name:
    5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
    EC=2.1.1.14
Alternative name(s):
    Methionine synthase, vitamin-B12 independent isozyme
    Cobalamin-independent methionine synthase

Gene names

Name:	metE
Ordered Locus Names:	BA_4218, GBAA_4218, BAS3912

Organism

Bacillus anthracis [Complete proteome] [HAMAP]

Taxonomic identifier

1392 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	762 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity.
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 762	762	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_0000098609
Sites
	Metal binding	645	1	Zinc By similarity
	Metal binding	647	1	Zinc By similarity
	Metal binding	730	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6KNA9-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 6FBC8EB3CF60C349	FASTA	762	87,232
	10 20 30 40 50 60 MAIQTSNLGY PRIGLQREWK KTLEAFWSNK INEEQFLTTM KEIRLQHVKV QQEKGIELIP 70 80 90 100 110 120 IGDFTYYDHV LDTAYMLGFI PSRFSEFTSY LDVYFAMARG SKDHVASEMT KWFNTNYHYI 130 140 150 160 170 180 VPEYEEGLQI SLKDNRPLRL YEEAKQELGV DGKPVILGPY TFLKLAKGYT QEQFATILKQ 190 200 210 220 230 240 LVAPYVQLLS ELHAAGAQII QVDEPIFASL TKEEVQQAKE IYEAIRKEVP NATLLLQTYF 250 260 270 280 290 300 DSVEENYEEI ITFPVSSIGL DFVHGKEGNL NAISKYGFPA DKTLAVGCID GRNIWRADLD 310 320 330 340 350 360 EVLTLFTTLQ KQVQTKDLIV QPSCSLLHTP IDKTEETHLS TELFDALAFA NQKLEELVLI 370 380 390 400 410 420 HSALTQGTES ISNELETYRN VHHTIRSSAA RNREDVKAAR TALKEEDFSR PLPFEKRYEL 430 440 450 460 470 480 QQVALKLPLL PTTTIGSFPQ TTEVRQTRKE WRNGIISNEQ YEQFIEKETE KWIRYQEEIG 490 500 510 520 530 540 LDVLVHGEFE RTDMVEYFGE RLAGFSFTKN GWVQSYGSRC VKPPVIYGDV AFINGMTIKE 550 560 570 580 590 600 TVYAQSLTEK VVKGMLTGPV TILNWSFVRN DIPRKEVSYQ IALALRHEIE LLESSGIRVI 610 620 630 640 650 660 QVDEPALREG MPLKEKDWDA YITWAVQSFL LATSSVANET QIHTHMCYSN FEDIVDAIRA 670 680 690 700 710 720 LDADVISIET SRSHGEFIDT LKHTTYEKGI GLGVYDIHSP RVPSKDEMYK IVEQSLQVCD 730 740 750 760 PKYFWINPDC GLKTRRTEEV IPALEHMVQA AKDARSLLKT NA

References

[1]	"The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria." Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J. , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M. Nature 423:81-86(2003) [PubMed: 12721629] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames / isolate Porton.
[2]	"Bacillus anthracis comparative genomics." Ravel J., Rasko D.A., Shumway M.F., Jiang L., Cer R.Z., Federova N.B., Wilson M., Stanley S., Decker S., Read T.D., Salzberg S.L., Fraser C.M. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames ancestor.
[3]	"Complete genome sequence of Bacillus anthracis Sterne." Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H. Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sterne.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016879 Genomic DNA. Translation: AAP27939.1. AE017334 Genomic DNA. Translation: AAT33335.1. AE017225 Genomic DNA. Translation: AAT56213.1.
RefSeq	NP_846453.1. YP_020860.1. YP_030162.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1088935. 2818200. 2850686.
GenomeReviews	Gene locus BA_4218 in contig AE016879_GR. Gene locus BAS3912 in contig AE017225_GR. Gene locus GBAA_4218 in contig AE017334_GR.
KEGG	ban:BA4218. bar:GBAA4218. bat:BAS3912.
TIGR	BA_4218. GBAA_4218.
Phylogenomic databases
HOGENOM	Q6KNA9.
OMA	CSLLHTP.
Enzyme and pathway databases
BioCyc	BANT260799:BAS3912-MON. BANT261594:GBAA4218-MON.
BRENDA	2.1.1.14. 267517.
Family and domain databases
HAMAP	MF_00172. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR002629. Methionine_synth. IPR006276. MeTrfase_B12_ind. [Graphical view]
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
ProDom	PD004692. Methionine_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01371. met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_BACAN

Accession

Primary (citable) accession number: Q6KNA9
Secondary accession number(s): Q6HU26, Q81MM9

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	July 5, 2004
Last modified:	November 3, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)