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Protein

5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase

Gene

metE

Organism
Bacillus anthracis
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation.UniRule annotation

Catalytic activityi

5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Pathwayi: L-methionine biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes L-methionine from L-homocysteine (MetE route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE), 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE), 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE)
This subpathway is part of the pathway L-methionine biosynthesis via de novo pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-methionine from L-homocysteine (MetE route), the pathway L-methionine biosynthesis via de novo pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi645 – 6451ZincUniRule annotation
Metal bindingi647 – 6471ZincUniRule annotation
Metal bindingi730 – 7301ZincUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciANTHRA:METE-MONOMER.
BANT260799:GJAJ-3969-MONOMER.
BANT261594:GJ7F-4102-MONOMER.
UniPathwayiUPA00051; UER00082.

Names & Taxonomyi

Protein namesi
Recommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferaseUniRule annotation (EC:2.1.1.14UniRule annotation)
Alternative name(s):
Cobalamin-independent methionine synthaseUniRule annotation
Methionine synthase, vitamin-B12 independent isozymeUniRule annotation
Gene namesi
Name:metEUniRule annotation
Ordered Locus Names:BA_4218, GBAA_4218, BAS3912
OrganismiBacillus anthracis
Taxonomic identifieri1392 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
Proteomesi
  • UP000000594 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7627625-methyltetrahydropteroyltriglutamate--homocysteine methyltransferasePRO_0000098609Add
BLAST

Interactioni

Protein-protein interaction databases

IntActiQ6KNA9. 21 interactions.
STRINGi198094.BA_4218.

Structurei

3D structure databases

ProteinModelPortaliQ6KNA9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the vitamin-B12 independent methionine synthase family.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4107QYE. Bacteria.
COG0620. LUCA.
HOGENOMiHOG000246221.
KOiK00549.
OMAiRFGWVQS.
OrthoDBiEOG6FFS3G.

Family and domain databases

HAMAPiMF_00172. Meth_synth.
InterProiIPR013215. Cbl-indep_Met_Synth_N.
IPR006276. Cobalamin-indep_Met_synthase.
IPR002629. Met_Synth_C/arc.
[Graphical view]
PfamiPF08267. Meth_synt_1. 1 hit.
PF01717. Meth_synt_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMsiTIGR01371. met_syn_B12ind. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6KNA9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAIQTSNLGY PRIGLQREWK KTLEAFWSNK INEEQFLTTM KEIRLQHVKV
60 70 80 90 100
QQEKGIELIP IGDFTYYDHV LDTAYMLGFI PSRFSEFTSY LDVYFAMARG
110 120 130 140 150
SKDHVASEMT KWFNTNYHYI VPEYEEGLQI SLKDNRPLRL YEEAKQELGV
160 170 180 190 200
DGKPVILGPY TFLKLAKGYT QEQFATILKQ LVAPYVQLLS ELHAAGAQII
210 220 230 240 250
QVDEPIFASL TKEEVQQAKE IYEAIRKEVP NATLLLQTYF DSVEENYEEI
260 270 280 290 300
ITFPVSSIGL DFVHGKEGNL NAISKYGFPA DKTLAVGCID GRNIWRADLD
310 320 330 340 350
EVLTLFTTLQ KQVQTKDLIV QPSCSLLHTP IDKTEETHLS TELFDALAFA
360 370 380 390 400
NQKLEELVLI HSALTQGTES ISNELETYRN VHHTIRSSAA RNREDVKAAR
410 420 430 440 450
TALKEEDFSR PLPFEKRYEL QQVALKLPLL PTTTIGSFPQ TTEVRQTRKE
460 470 480 490 500
WRNGIISNEQ YEQFIEKETE KWIRYQEEIG LDVLVHGEFE RTDMVEYFGE
510 520 530 540 550
RLAGFSFTKN GWVQSYGSRC VKPPVIYGDV AFINGMTIKE TVYAQSLTEK
560 570 580 590 600
VVKGMLTGPV TILNWSFVRN DIPRKEVSYQ IALALRHEIE LLESSGIRVI
610 620 630 640 650
QVDEPALREG MPLKEKDWDA YITWAVQSFL LATSSVANET QIHTHMCYSN
660 670 680 690 700
FEDIVDAIRA LDADVISIET SRSHGEFIDT LKHTTYEKGI GLGVYDIHSP
710 720 730 740 750
RVPSKDEMYK IVEQSLQVCD PKYFWINPDC GLKTRRTEEV IPALEHMVQA
760
AKDARSLLKT NA
Length:762
Mass (Da):87,232
Last modified:July 5, 2004 - v1
Checksum:i6FBC8EB3CF60C349
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016879 Genomic DNA. Translation: AAP27939.1.
AE017334 Genomic DNA. Translation: AAT33335.1.
AE017225 Genomic DNA. Translation: AAT56213.1.
RefSeqiNP_846453.1. NC_003997.3.
WP_001007635.1. NZ_LHUO01000006.1.
YP_030162.1. NC_005945.1.

Genome annotation databases

EnsemblBacteriaiAAP27939; AAP27939; BA_4218.
AAT33335; AAT33335; GBAA_4218.
AAT56213; AAT56213; BAS3912.
GeneIDi1088935.
2850686.
KEGGiban:BA_4218.
bar:GBAA_4218.
bat:BAS3912.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016879 Genomic DNA. Translation: AAP27939.1.
AE017334 Genomic DNA. Translation: AAT33335.1.
AE017225 Genomic DNA. Translation: AAT56213.1.
RefSeqiNP_846453.1. NC_003997.3.
WP_001007635.1. NZ_LHUO01000006.1.
YP_030162.1. NC_005945.1.

3D structure databases

ProteinModelPortaliQ6KNA9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ6KNA9. 21 interactions.
STRINGi198094.BA_4218.

Protocols and materials databases

DNASUi1088935.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAP27939; AAP27939; BA_4218.
AAT33335; AAT33335; GBAA_4218.
AAT56213; AAT56213; BAS3912.
GeneIDi1088935.
2850686.
KEGGiban:BA_4218.
bar:GBAA_4218.
bat:BAS3912.

Phylogenomic databases

eggNOGiENOG4107QYE. Bacteria.
COG0620. LUCA.
HOGENOMiHOG000246221.
KOiK00549.
OMAiRFGWVQS.
OrthoDBiEOG6FFS3G.

Enzyme and pathway databases

UniPathwayiUPA00051; UER00082.
BioCyciANTHRA:METE-MONOMER.
BANT260799:GJAJ-3969-MONOMER.
BANT261594:GJ7F-4102-MONOMER.

Family and domain databases

HAMAPiMF_00172. Meth_synth.
InterProiIPR013215. Cbl-indep_Met_Synth_N.
IPR006276. Cobalamin-indep_Met_synthase.
IPR002629. Met_Synth_C/arc.
[Graphical view]
PfamiPF08267. Meth_synt_1. 1 hit.
PF01717. Meth_synt_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMsiTIGR01371. met_syn_B12ind. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria."
    Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J.
    , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M.
    Nature 423:81-86(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Ames / isolate Porton.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Ames ancestor.
  3. "Complete genome sequence of Bacillus anthracis Sterne."
    Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H.
    Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Sterne.

Entry informationi

Entry nameiMETE_BACAN
AccessioniPrimary (citable) accession number: Q6KNA9
Secondary accession number(s): Q6HU26, Q81MM9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: July 5, 2004
Last modified: January 20, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.