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Q6KIT3 (SYE_MYCMO) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate--tRNA ligase

EC=6.1.1.17
Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS
Gene names
Name:gltX
Ordered Locus Names:MMOB0050
OrganismMycoplasma mobile (strain ATCC 43663 / 163K / NCTC 11711) [Complete proteome] [HAMAP]
Taxonomic identifier267748 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

Protein attributes

Sequence length464 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP-Rule MF_00022

Catalytic activity

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP-Rule MF_00022

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00022

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00022.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglutamyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

glutamate-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 464464Glutamate--tRNA ligase HAMAP-Rule MF_00022
PRO_0000119604

Regions

Motif12 – 2211"HIGH" region HAMAP-Rule MF_00022
Motif254 – 2585"KMSKS" region HAMAP-Rule MF_00022

Sites

Binding site2571ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6KIT3 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 1D1298BC72D3B524

FASTA46454,385
        10         20         30         40         50         60 
MKKTRIRTRY APSPTGYLHI GGARTALFNY LFAKHFNGDF IVRIEDTDIA RNVAGGEESQ 

        70         80         90        100        110        120 
LDNLEWLQIY PDESPKNPNE KYGKYRQSEK LDRYNEIVEI LLKKGLAYKA YDTTYELEKQ 

       130        140        150        160        170        180 
RAEQIAKGIF SFRYDRNWLK ISDEEIKKRE VEQTYSIRIA LPKNHDYEWN DLVRGLIKVN 

       190        200        210        220        230        240 
SEDIGDWVII KSDKYPTYNF AVVVDDFDMQ ISHVLRGEEH ITNTPKQLAV YEAMGWDKPV 

       250        260        270        280        290        300 
FGHLTLITNS KGVKLSKRDD SVKQFISNYK EDGYVSWAIS NYLALLGWTS KDTKEIMTKE 

       310        320        330        340        350        360 
ELIEKFDPER LSASPSKFDM KKMNWYGKHY LQEINKNEIF EYLESLKDKK WLDLFIETFL 

       370        380        390        400        410        420 
PNAFSLSELK RELKEYENPM IEKPEIEIND VVKKFKQNLN FENFSVDSIQ KAIDKTGTDL 

       430        440        450        460 
NVNGKKLFLP IRLATTFNEH GPELAKAIYL YGKEIIQKRL GNVN 

« Hide

References

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE017308 Genomic DNA. Translation: AAT27491.1.
RefSeqYP_015702.1. NC_006908.1.

3D structure databases

ProteinModelPortalQ6KIT3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING267748.MMOB0050.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAT27491; AAT27491; MMOB0050.
GeneID2807581.
KEGGmmo:MMOB0050.
PATRIC20015283. VBIMycMob32482_0005.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0008.
HOGENOMHOG000252720.
KOK01885.
OMAWLPEEMG.
OrthoDBEOG6DRPF7.

Enzyme and pathway databases

BioCycMMOB267748:GH6Y-5-MONOMER.

Family and domain databases

Gene3D1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPMF_00022_B. Glu_tRNA_synth_B.
InterProIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERPTHR10119. PTHR10119. 1 hit.
PfamPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSPR00987. TRNASYNTHGLU.
SUPFAMSSF48163. SSF48163. 1 hit.
TIGRFAMsTIGR00464. gltX_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYE_MYCMO
AccessionPrimary (citable) accession number: Q6KIT3
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: July 5, 2004
Last modified: May 14, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries