Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q6KHX0 (PFKA_MYCMO) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ATP-dependent 6-phosphofructokinase

Short name=ATP-PFK
Short name=Phosphofructokinase
EC=2.7.1.11
Alternative name(s):
Phosphohexokinase
Gene names
Name:pfkA
Ordered Locus Names:MMOB3200
OrganismMycoplasma mobile (strain ATCC 43663 / 163K / NCTC 11711) [Complete proteome] [HAMAP]
Taxonomic identifier267748 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

Protein attributes

Sequence length325 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis By similarity. HAMAP-Rule MF_00339

Catalytic activity

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. HAMAP-Rule MF_00339

Cofactor

Magnesium By similarity. HAMAP-Rule MF_00339

Enzyme regulation

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate By similarity. HAMAP-Rule MF_00339

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. HAMAP-Rule MF_00339

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_00339

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00339.

Sequence similarities

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 325325ATP-dependent 6-phosphofructokinase HAMAP-Rule MF_00339
PRO_1000059782

Regions

Nucleotide binding73 – 742ATP By similarity
Nucleotide binding103 – 1064ATP By similarity
Region22 – 265Allosteric activator ADP binding; shared with dimeric partner By similarity
Region126 – 1283Substrate binding By similarity
Region170 – 1723Substrate binding By similarity
Region186 – 1883Allosteric activator ADP binding By similarity
Region215 – 2173Allosteric activator ADP binding By similarity
Region252 – 2554Substrate binding By similarity

Sites

Active site1281Proton acceptor By similarity
Metal binding1041Magnesium; catalytic By similarity
Binding site121ATP; via amide nitrogen By similarity
Binding site1551Allosteric activator ADP By similarity
Binding site2131Allosteric activator ADP By similarity
Binding site2241Substrate By similarity
Binding site2461Substrate; shared with dimeric partner By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6KHX0 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 2110A703E6D31D1D

FASTA32535,000
        10         20         30         40         50         60 
MAKKIAILTS GGDSPGMNNA IRTIVKTSKI HGIEVFLVYN GYKGLVEKTI KNANEINVDQ 

        70         80         90        100        110        120 
YIGSGGTFIG SARYLEFKKL EVRQKAVQNL KEMGIDSLVV IGGDGSYAGA QLLHELGVKT 

       130        140        150        160        170        180 
IGLPGTIDND IASTEYTIGL DTALNTIVEN VDRLRDTMNS MNMVALVEVM GHGAGDLAML 

       190        200        210        220        230        240 
SGLATGAEII VTNAHRKSID EMIEIVKDQM IAKTKRSVIG IVSEFIYDDL KKVAKEIEDK 

       250        260        270        280        290        300 
TGIRTRAIIL AHTQRGGNPK AYERINASFL GIAAVESLLK NESGVALGFK GNKIISTPIP 

       310        320 
EALKAQNSAK NENELVTKIN KINQS 

« Hide

References

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE017308 Genomic DNA. Translation: AAT27806.1.
RefSeqYP_016017.1. NC_006908.1.

3D structure databases

ProteinModelPortalQ6KHX0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING267748.MMOB3200.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAT27806; AAT27806; MMOB3200.
GeneID2807302.
KEGGmmo:MMOB3200.
PATRIC20015975. VBIMycMob32482_0338.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0205.
HOGENOMHOG000248870.
KOK00850.
OMANVMIGIR.
OrthoDBEOG644ZRM.

Enzyme and pathway databases

BioCycMMOB267748:GH6Y-334-MONOMER.
UniPathwayUPA00109; UER00182.

Family and domain databases

HAMAPMF_00339. Phosphofructokinase_I_B1.
InterProIPR012003. ATP_PFK_prok.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamPF00365. PFK. 1 hit.
[Graphical view]
PIRSFPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSPR00476. PHFRCTKINASE.
SUPFAMSSF53784. SSF53784. 1 hit.
TIGRFAMsTIGR02482. PFKA_ATP. 1 hit.
PROSITEPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePFKA_MYCMO
AccessionPrimary (citable) accession number: Q6KHX0
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: July 5, 2004
Last modified: July 9, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways