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Q6KHN7

- SYI_MYCMO

UniProt

Q6KHN7 - SYI_MYCMO

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Mycoplasma mobile (strain ATCC 43663 / 163K / NCTC 11711)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei550 – 5501Aminoacyl-adenylateUniRule annotation
Binding sitei594 – 5941ATPUniRule annotation
Metal bindingi861 – 8611ZincUniRule annotation
Metal bindingi864 – 8641ZincUniRule annotation
Metal bindingi877 – 8771ZincUniRule annotation
Metal bindingi880 – 8801ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciMMOB267748:GH6Y-430-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:MMOB4070
OrganismiMycoplasma mobile (strain ATCC 43663 / 163K / NCTC 11711)
Taxonomic identifieri267748 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000009072: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 885885Isoleucine--tRNA ligasePRO_0000098421Add
BLAST

Proteomic databases

PRIDEiQ6KHN7.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi267748.MMOB4070.

Structurei

3D structure databases

ProteinModelPortaliQ6KHN7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi59 – 6911"HIGH" regionAdd
BLAST
Motifi591 – 5955"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKKRIELM.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6KHN7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKNKYKETLN IPQDVLPMRA NLIEKELKFK TFWDQNKIYQ KALEKNKTNK
60 70 80 90 100
PFILHDGPPY ANGDLHIGHA LNKILKDIVV RYKSLSGFYA PFVPGWDTHG
110 120 130 140 150
LPIENKILKE LKIDDHKNIS TLELRKQARE YALSQIKNQK KQFLTMQLFS
160 170 180 190 200
DFKDYYQTLD PKFEAEQLKV FKKMALDGLI YKSLQPVFWS PSSQSALAEA
210 220 230 240 250
EVEYHEHKSH SIYTAFKIVK GNKFVEKNDF LVIWTTTPWT LIANSGVSVK
260 270 280 290 300
EEFSYSKINY ENKNYIFATD LVQKVTEIFG WKNFKIISSF QGKDLVGIEY
310 320 330 340 350
QRPIKQEKTA PIVLGHHVTL ESGSGLVHMA PLFGLDDFII GRVEKLEEIM
360 370 380 390 400
HINDDGSINE FGNQFANKFY WDANPEINEF LKQNNLLLHH SFLYHSYPHD
410 420 430 440 450
WRTNKPVIYR GSNQWFVSID PIKEKITKSI EHVESYPTWG ISRLKTMIDN
460 470 480 490 500
RTSWTISRQR AWGVPIPIFY NEKNEPVFEE ELFDHIIDLV SKYGTDIWFE
510 520 530 540 550
KSTDELLPEK YKNKNWTKEK DIMDVWFDSG TTSMGVKIEG APVPWDVYLE
560 570 580 590 600
GSDQYRGWFN SSLINSVVYY NQAPFKKLVS HGFVLDEKGN KMSKSKGNVV
610 620 630 640 650
LPQEIISKFG ADILRLWVAN SEYTSDVTIG ENIINQNVEI YRKFRNTFRF
660 670 680 690 700
LIANLVDFDY KKDLKELTGI HLLIKEQLET LKFNTKIAYE NFQFTNVIKL
710 720 730 740 750
TNNFVYNLSS FYLDFSKDIL YANKIEDLQR KMIQTNFYNI TKTLLLILAP
760 770 780 790 800
ILPTTIEEVY QIFDQANKKE SVHLENFFGI SSIETIQEEK WNSFFELKNK
810 820 830 840 850
VYALIEIAIK DKLIKSANQA LVYIKETDEF LKTLDLKNLL MVGKVIFSKE
860 870 880
NKIENYEGHL CERCRIYFDF IDKDNLCVRC SKVIL
Length:885
Mass (Da):103,616
Last modified:July 5, 2004 - v1
Checksum:iA0C5A0DAB008E2AF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017308 Genomic DNA. Translation: AAT27893.1.
RefSeqiYP_016104.1. NC_006908.1.

Genome annotation databases

EnsemblBacteriaiAAT27893; AAT27893; MMOB4070.
GeneIDi2807754.
KEGGimmo:MMOB4070.
PATRICi20016173. VBIMycMob32482_0429.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017308 Genomic DNA. Translation: AAT27893.1 .
RefSeqi YP_016104.1. NC_006908.1.

3D structure databases

ProteinModelPortali Q6KHN7.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 267748.MMOB4070.

Proteomic databases

PRIDEi Q6KHN7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAT27893 ; AAT27893 ; MMOB4070 .
GeneIDi 2807754.
KEGGi mmo:MMOB4070.
PATRICi 20016173. VBIMycMob32482_0429.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KKRIELM.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci MMOB267748:GH6Y-430-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43663 / 163K / NCTC 11711.

Entry informationi

Entry nameiSYI_MYCMO
AccessioniPrimary (citable) accession number: Q6KHN7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: July 5, 2004
Last modified: November 26, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3