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Q6KHN7

- SYI_MYCMO

UniProt

Q6KHN7 - SYI_MYCMO

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Mycoplasma mobile (strain ATCC 43663 / 163K / NCTC 11711)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei550 – 5501Aminoacyl-adenylateUniRule annotation
    Binding sitei594 – 5941ATPUniRule annotation
    Metal bindingi861 – 8611ZincUniRule annotation
    Metal bindingi864 – 8641ZincUniRule annotation
    Metal bindingi877 – 8771ZincUniRule annotation
    Metal bindingi880 – 8801ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciMMOB267748:GH6Y-430-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:MMOB4070
    OrganismiMycoplasma mobile (strain ATCC 43663 / 163K / NCTC 11711)
    Taxonomic identifieri267748 [NCBI]
    Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
    ProteomesiUP000009072: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 885885Isoleucine--tRNA ligasePRO_0000098421Add
    BLAST

    Proteomic databases

    PRIDEiQ6KHN7.

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi267748.MMOB4070.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6KHN7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi59 – 6911"HIGH" regionAdd
    BLAST
    Motifi591 – 5955"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKKRIELM.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q6KHN7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKNKYKETLN IPQDVLPMRA NLIEKELKFK TFWDQNKIYQ KALEKNKTNK    50
    PFILHDGPPY ANGDLHIGHA LNKILKDIVV RYKSLSGFYA PFVPGWDTHG 100
    LPIENKILKE LKIDDHKNIS TLELRKQARE YALSQIKNQK KQFLTMQLFS 150
    DFKDYYQTLD PKFEAEQLKV FKKMALDGLI YKSLQPVFWS PSSQSALAEA 200
    EVEYHEHKSH SIYTAFKIVK GNKFVEKNDF LVIWTTTPWT LIANSGVSVK 250
    EEFSYSKINY ENKNYIFATD LVQKVTEIFG WKNFKIISSF QGKDLVGIEY 300
    QRPIKQEKTA PIVLGHHVTL ESGSGLVHMA PLFGLDDFII GRVEKLEEIM 350
    HINDDGSINE FGNQFANKFY WDANPEINEF LKQNNLLLHH SFLYHSYPHD 400
    WRTNKPVIYR GSNQWFVSID PIKEKITKSI EHVESYPTWG ISRLKTMIDN 450
    RTSWTISRQR AWGVPIPIFY NEKNEPVFEE ELFDHIIDLV SKYGTDIWFE 500
    KSTDELLPEK YKNKNWTKEK DIMDVWFDSG TTSMGVKIEG APVPWDVYLE 550
    GSDQYRGWFN SSLINSVVYY NQAPFKKLVS HGFVLDEKGN KMSKSKGNVV 600
    LPQEIISKFG ADILRLWVAN SEYTSDVTIG ENIINQNVEI YRKFRNTFRF 650
    LIANLVDFDY KKDLKELTGI HLLIKEQLET LKFNTKIAYE NFQFTNVIKL 700
    TNNFVYNLSS FYLDFSKDIL YANKIEDLQR KMIQTNFYNI TKTLLLILAP 750
    ILPTTIEEVY QIFDQANKKE SVHLENFFGI SSIETIQEEK WNSFFELKNK 800
    VYALIEIAIK DKLIKSANQA LVYIKETDEF LKTLDLKNLL MVGKVIFSKE 850
    NKIENYEGHL CERCRIYFDF IDKDNLCVRC SKVIL 885
    Length:885
    Mass (Da):103,616
    Last modified:July 5, 2004 - v1
    Checksum:iA0C5A0DAB008E2AF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017308 Genomic DNA. Translation: AAT27893.1.
    RefSeqiWP_011264927.1. NC_006908.1.
    YP_016104.1. NC_006908.1.

    Genome annotation databases

    EnsemblBacteriaiAAT27893; AAT27893; MMOB4070.
    GeneIDi2807754.
    KEGGimmo:MMOB4070.
    PATRICi20016173. VBIMycMob32482_0429.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017308 Genomic DNA. Translation: AAT27893.1 .
    RefSeqi WP_011264927.1. NC_006908.1.
    YP_016104.1. NC_006908.1.

    3D structure databases

    ProteinModelPortali Q6KHN7.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 267748.MMOB4070.

    Proteomic databases

    PRIDEi Q6KHN7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAT27893 ; AAT27893 ; MMOB4070 .
    GeneIDi 2807754.
    KEGGi mmo:MMOB4070.
    PATRICi 20016173. VBIMycMob32482_0429.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KKRIELM.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci MMOB267748:GH6Y-430-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43663 / 163K / NCTC 11711.

    Entry informationi

    Entry nameiSYI_MYCMO
    AccessioniPrimary (citable) accession number: Q6KHN7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 76 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3