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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Mycoplasma mobile (strain ATCC 43663 / 163K / NCTC 11711)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei34S-adenosyl-L-methionineUniRule annotation1
Binding sitei59S-adenosyl-L-methionineUniRule annotation1
Binding sitei86S-adenosyl-L-methionineUniRule annotation1
Active sitei107By similarity1
Binding sitei107S-adenosyl-L-methionineUniRule annotation1
Binding sitei111SubstrateUniRule annotation1
Binding sitei143SubstrateUniRule annotation1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:MMOB4960
OrganismiMycoplasma mobile (strain ATCC 43663 / 163K / NCTC 11711)
Taxonomic identifieri267748 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
Proteomesi
  • UP000009072 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001713551 – 201tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST201

Interactioni

Protein-protein interaction databases

STRINGi267748.MMOB4960.

Structurei

3D structure databases

ProteinModelPortaliQ6KHE8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni181 – 184Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107T6Z. Bacteria.
COG0220. LUCA.
HOGENOMiHOG000251689.
KOiK03439.
OMAiAHPEINY.
OrthoDBiPOG091H015P.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6KHE8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVRRDKNLE KILDVDPLVI SKFPFKLKQD TIIELGSGKG TMISALALKN
60 70 80 90 100
PSQNYLCVER DRTIASKAIE KFNDLNLKNI NLIVSDIQHL TEIIENKVNT
110 120 130 140 150
IWLTFSDPWP KNRHEHRRLT YKTFLDLYKK FLSPQGVIKL KTDNDKFFHY
160 170 180 190 200
SLESMSEFGM KILYQTNDLH NSIKNLDNEM TDYEKKWSSL GKSINYLEAS

F
Length:201
Mass (Da):23,579
Last modified:July 5, 2004 - v1
Checksum:iB98F0D6F7B802E5B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017308 Genomic DNA. Translation: AAT27982.1.
RefSeqiWP_011265016.1. NC_006908.1.

Genome annotation databases

EnsemblBacteriaiAAT27982; AAT27982; MMOB4960.
KEGGimmo:MMOB4960.
PATRICi20016375. VBIMycMob32482_0523.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017308 Genomic DNA. Translation: AAT27982.1.
RefSeqiWP_011265016.1. NC_006908.1.

3D structure databases

ProteinModelPortaliQ6KHE8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi267748.MMOB4960.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAT27982; AAT27982; MMOB4960.
KEGGimmo:MMOB4960.
PATRICi20016375. VBIMycMob32482_0523.

Phylogenomic databases

eggNOGiENOG4107T6Z. Bacteria.
COG0220. LUCA.
HOGENOMiHOG000251689.
KOiK03439.
OMAiAHPEINY.
OrthoDBiPOG091H015P.

Enzyme and pathway databases

UniPathwayiUPA00989.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRMB_MYCMO
AccessioniPrimary (citable) accession number: Q6KHE8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 5, 2004
Last modified: November 2, 2016
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.