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Protein

Beta-1,3-N-acetylglucosaminyltransferase radical fringe

Gene

rfng

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Glycosyltransferase that initiates the elongation of O-linked fucose residues attached to EGF-like repeats in the extracellular domain of Notch molecules (By similarity). Regulates neurogenesis in the hindbrain; involved in boundary cell specification via Notch-mediated activation of wnt1 expression.By similarity2 Publications

Catalytic activityi

Transfers a beta-D-GlcNAc residue from UDP-D-GlcNAc to the fucose residue of a fucosylated protein acceptor.By similarity

Cofactori

Mn2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei111 – 1111SubstrateBy similarity
Binding sitei184 – 1841SubstrateBy similarity
Metal bindingi185 – 1851ManganeseBy similarity
Active sitei273 – 2731By similarity
Metal bindingi297 – 2971ManganeseBy similarity

GO - Molecular functioni

GO - Biological processi

  • brain segmentation Source: ZFIN
  • cell differentiation Source: UniProtKB-KW
  • fourth ventricle development Source: ZFIN
  • hindbrain development Source: ZFIN
  • Notch signaling pathway Source: UniProtKB-KW
  • rhombomere boundary formation Source: ZFIN
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Glycosyltransferase, Transferase

Keywords - Biological processi

Differentiation, Neurogenesis, Notch signaling pathway

Keywords - Ligandi

Manganese, Metal-binding

Protein family/group databases

CAZyiGT31. Glycosyltransferase Family 31.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,3-N-acetylglucosaminyltransferase radical fringe (EC:2.4.1.222)
Alternative name(s):
O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
Gene namesi
Name:rfngImported
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Unplaced

Organism-specific databases

ZFINiZDB-GENE-030131-5418. rfng.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1111CytoplasmicSequence analysisAdd
BLAST
Transmembranei12 – 3221Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini33 – 362330LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 362362Beta-1,3-N-acetylglucosaminyltransferase radical fringePRO_0000250423Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi150 – 1501N-linked (GlcNAc...)Sequence analysis
Disulfide bondi151 ↔ 162By similarity
Disulfide bondi180 ↔ 243By similarity
Disulfide bondi347 ↔ 356By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ6KFX9.
PRIDEiQ6KFX9.

Expressioni

Developmental stagei

Expressed both maternally and zygotically. At the tailbud stage, expressed weakly in the adaxial cells and the future brain. From the 3-somite stage, strong expression in the adaxial cells and weak expression in the somites and tailbud. During somitogenesis, expression in the brain is gradually restricted to and up-regulated in the tectum and rhombomere boundary cells.3 Publications

Interactioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000105906.

Structurei

3D structure databases

ProteinModelPortaliQ6KFX9.
SMRiQ6KFX9. Positions 92-358.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 31 family.Sequence analysis

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410II9W. Eukaryota.
ENOG410XS8Y. LUCA.
HOGENOMiHOG000046678.
HOVERGENiHBG007986.
InParanoidiQ6KFX9.
PhylomeDBiQ6KFX9.

Family and domain databases

InterProiIPR017374. Fringe.
IPR003378. Fringe-like.
[Graphical view]
PfamiPF02434. Fringe. 1 hit.
[Graphical view]
PIRSFiPIRSF038073. B-acetylgalactosaminyltfrase. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6KFX9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHLSHVASNK TCFLLSLAFC ALLVLLIPSL QPPQRQADLP QPRPHAKPAK
60 70 80 90 100
HPLKNSLYRP NEDTRGGGFT QTPPPDNLEK MDDLNSHYRD FLDLRDIFIA
110 120 130 140 150
VKTTRKYHKS RLQLLSQTWV SRAKEQTFIF TDGEDKELRL KAGLNIINTN
160 170 180 190 200
CSAAHTRQAL CCKMSVEYDK FIESQKKWFC HVDDDNYVIL PSLLELLSSY
210 220 230 240 250
SHTQDVYLGR PSLDHPIEAA ERVKSDGSVS VKFWFATGGA GFCISRGLAL
260 270 280 290 300
KMSPWASLGN FITTAEKIRL PDDCTIGYII EALLEVPLTH TGLFHSHLEN
310 320 330 340 350
LQRLPAENIL RQVTLSYGGF ENRRNVVSVG GAFSLAEDPT RFKTVHCKLY
360
PDTEWCPRKT RH
Length:362
Mass (Da):41,019
Last modified:July 5, 2004 - v1
Checksum:iA6CDCBFBC84C69C7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF510993 mRNA. Translation: AAQ08017.1.
UniGeneiDr.11937.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF510993 mRNA. Translation: AAQ08017.1.
UniGeneiDr.11937.

3D structure databases

ProteinModelPortaliQ6KFX9.
SMRiQ6KFX9. Positions 92-358.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000105906.

Protein family/group databases

CAZyiGT31. Glycosyltransferase Family 31.

Proteomic databases

PaxDbiQ6KFX9.
PRIDEiQ6KFX9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

ZFINiZDB-GENE-030131-5418. rfng.

Phylogenomic databases

eggNOGiENOG410II9W. Eukaryota.
ENOG410XS8Y. LUCA.
HOGENOMiHOG000046678.
HOVERGENiHBG007986.
InParanoidiQ6KFX9.
PhylomeDBiQ6KFX9.

Miscellaneous databases

PROiQ6KFX9.

Family and domain databases

InterProiIPR017374. Fringe.
IPR003378. Fringe-like.
[Graphical view]
PfamiPF02434. Fringe. 1 hit.
[Graphical view]
PIRSFiPIRSF038073. B-acetylgalactosaminyltfrase. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRFNG_DANRE
AccessioniPrimary (citable) accession number: Q6KFX9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: July 5, 2004
Last modified: July 6, 2016
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.