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Protein

Pleckstrin homology domain-containing family G member 2

Gene

Plekhg2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

May be a transforming oncogene with exchange activity for CDC42. May be a guanine-nucleotide exchange factor (GEF) for RAC1 and CDC42. Activated by the binding to subunits beta and gamma of the heterotrimeric guanine nucleotide-binding protein (G protein) (By similarity).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Names & Taxonomyi

Protein namesi
Recommended name:
Pleckstrin homology domain-containing family G member 2
Short name:
PH domain-containing family G member 2
Alternative name(s):
Common site lymphoma/leukemia guanine nucleotide exchange factor
Short name:
Common site lymphoma/leukemia GEF
Gene namesi
Name:Plekhg2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:2141874. Plekhg2.

Pathology & Biotechi

Keywords - Diseasei

Tumor suppressor

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13401340Pleckstrin homology domain-containing family G member 2PRO_0000306862Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei441 – 4411PhosphothreonineBy similarity
Modified residuei446 – 4461PhosphoserineBy similarity
Modified residuei1215 – 12151PhosphothreonineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ6KAU7.
MaxQBiQ6KAU7.
PaxDbiQ6KAU7.
PRIDEiQ6KAU7.

PTM databases

iPTMnetiQ6KAU7.
PhosphoSiteiQ6KAU7.

Expressioni

Tissue specificityi

Expressed in thymus, skeletal muscle, lung, testis, uterus, pancreas and heart and also expressed during embryogenesis.1 Publication

Gene expression databases

BgeeiQ6KAU7.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000092228.

Structurei

3D structure databases

ProteinModelPortaliQ6KAU7.
SMRiQ6KAU7. Positions 100-406.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini98 – 279182DHPROSITE-ProRule annotationAdd
BLAST
Domaini309 – 40799PHPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi589 – 5946Poly-Glu
Compositional biasi1303 – 13064Poly-Gly

Sequence similaritiesi

Contains 1 DH (DBL-homology) domain.PROSITE-ProRule annotation
Contains 1 PH domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3518. Eukaryota.
ENOG410XR81. LUCA.
HOGENOMiHOG000169126.
HOVERGENiHBG108256.
InParanoidiQ6KAU7.

Family and domain databases

Gene3Di1.20.900.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR000219. DH-domain.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PfamiPF00169. PH. 1 hit.
PF00621. RhoGEF. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00325. RhoGEF. 1 hit.
[Graphical view]
SUPFAMiSSF48065. SSF48065. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50010. DH_2. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6KAU7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPEGARGLSL PKPSLRLGCG HQGEVCDCAA VSDTPTAQAA TTMASPRGSG
60 70 80 90 100
SSTSLSTVGS EGDPSPACSA SRPEPLPEPP IRLHLLPVGI QGSVKPSRLE
110 120 130 140 150
RVAREIVETE RAYVRDLRSI VEDYLGPLMD GRALGLNMEQ VGTLFANIED
160 170 180 190 200
IYEFSSELLE DLEGCSSAGG IAECFVQRSE DFDIYTLYCM NYPSSLALLR
210 220 230 240 250
ELSVSPPATL WLQERQAQLR HSLPLQSFLL KPVQRILKYH LLLQELGKHW
260 270 280 290 300
AEGPDSGGRE MVEEAIVSMT AVAWYINDMK RKQEHAARLQ EVQRRLGGWT
310 320 330 340 350
GPELSAFGEL VLEGTFRGGG GGGPRLRGGE RLLFLFSRML LVAKRRGPEY
360 370 380 390 400
TYKGHIFCCN LSVSETPRDP LGFKVSDLTI PKHRHLFQAK NQEEKRLWIH
410 420 430 440 450
CLQRLFFENH PASIPAKAKQ VLLENSLHCA PKSKHIPEPP TSPLDSPRPR
460 470 480 490 500
DAPGFTPGRR NPAPSPRLSG SRRGRRQSEP AKEAYVIFPQ NDKPQVKHAG
510 520 530 540 550
SEGELHPSSE LQPVSASGLP EDLEDAGPPT LDPSGTSITE EILELLNQRG
560 570 580 590 600
LRDSGPATHD IPKFPRDSRV PVESEPLPFQ SLPSRESSEE EEEEDLETDE
610 620 630 640 650
REPSPLHVLE GLEGSSAAEI PCIPSLTDIP SEVPSLPEIP EAPCLPCLSD
660 670 680 690 700
ISGVFEVPCL SPTSTVPDIP SLATTPSFPC GSWLPGPLQE AAQPQATRRE
710 720 730 740 750
LLSGSNPGRL SESPSESREG QEDDTEGVSF SAVQREAGTS VQGFPEELEY
760 770 780 790 800
RSCSEIRSAW QALEQGQLAR PGFPEPLLIL EDSDLRGGST SGKTGMPHSE
810 820 830 840 850
RSASRVRELA RLYSERIQQM QRAETRASTN APRRRPRVLA QPQPSPCPPQ
860 870 880 890 900
EEAEPGALPA FGHVLVCELA FPLNCTQESV PLGPAVLVQA ATPLCIQGDD
910 920 930 940 950
LSGQNLNVSD LSKQGHLSSN SIPPPVPLPG QSNFQNIQVP STSLLPKQEP
960 970 980 990 1000
PDVQVPTAST LPDTSQLQSQ VPAATPSAGH RNCVEIQVQS TTSLPGQECQ
1010 1020 1030 1040 1050
ADTVALSKQE GHEDSQNPNK APGAEQRDVS IDQGLAVVGG RPVSPLPVCT
1060 1070 1080 1090 1100
SSPDQQIPAT TPLPLSTDFP DMEGPGALPL PTQEGRPDCS IPCNPLPSLS
1110 1120 1130 1140 1150
QDVQVPAVIP VSQLQGLTDT RATVPLSSHK QEDAPECLGP EPSLTDTPAP
1160 1170 1180 1190 1200
RLLSSLGQQN TTDGPVSAAA VPLTEQGCSQ DLQGLITSPV QTTMELPKPR
1210 1220 1230 1240 1250
GLVSRVATSE SLDLTPPHSP SLSTRQLLGP SAAALSRYLA ASYISQSLAR
1260 1270 1280 1290 1300
RQGPGGEGTV ASQGHWSSSA PTSRAPSPPP QPQPPAPPAR RLSYATTVSI
1310 1320 1330 1340
QVGGGGRLRP AKAQVRLNHP ALLAAPHPGA VGPSQGPGGS
Length:1,340
Mass (Da):143,376
Last modified:October 2, 2007 - v2
Checksum:iE4FD7C9382658173
GO
Isoform 2 (identifier: Q6KAU7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-42: Missing.

Note: No experimental confirmation available.
Show »
Length:1,298
Mass (Da):139,196
Checksum:i151E2D6A4378CB95
GO
Isoform 3 (identifier: Q6KAU7-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     478-478: S → SGEYPPLEGDPGSQWPGLLTSFFLPP

Note: No experimental confirmation available.
Show »
Length:1,365
Mass (Da):146,060
Checksum:i619B5B6EB6D266D4
GO

Sequence cautioni

The sequence AAH52436.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAH56971.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence BAD21360.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti37 – 371A → AA in AAH56971 (PubMed:15489334).Curated
Sequence conflicti519 – 5191L → P in AAH52436 (PubMed:15489334).Curated
Sequence conflicti519 – 5191L → P in AAH56971 (PubMed:15489334).Curated
Sequence conflicti519 – 5191L → P in BAE25733 (PubMed:16141072).Curated
Sequence conflicti823 – 8253AET → TRP in AAH25625 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 4242Missing in isoform 2. 1 PublicationVSP_028531Add
BLAST
Alternative sequencei478 – 4781S → SGEYPPLEGDPGSQWPGLLT SFFLPP in isoform 3. 1 PublicationVSP_028532

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF465238 mRNA. Translation: AAL93134.1.
AK131110 mRNA. Translation: BAD21360.1. Different initiation.
BC025625 mRNA. Translation: AAH25625.1.
BC052436 mRNA. Translation: AAH52436.1. Different initiation.
BC056971 mRNA. Translation: AAH56971.1. Different initiation.
AK144152 mRNA. Translation: BAE25733.1.
CCDSiCCDS52162.1. [Q6KAU7-1]
RefSeqiNP_001077381.1. NM_001083912.1.
NP_001277471.1. NM_001290542.1.
NP_620091.2. NM_138752.2.
XP_006539504.1. XM_006539441.2.
XP_006539505.1. XM_006539442.2.
UniGeneiMm.235700.

Genome annotation databases

GeneIDi101497.
KEGGimmu:101497.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF465238 mRNA. Translation: AAL93134.1.
AK131110 mRNA. Translation: BAD21360.1. Different initiation.
BC025625 mRNA. Translation: AAH25625.1.
BC052436 mRNA. Translation: AAH52436.1. Different initiation.
BC056971 mRNA. Translation: AAH56971.1. Different initiation.
AK144152 mRNA. Translation: BAE25733.1.
CCDSiCCDS52162.1. [Q6KAU7-1]
RefSeqiNP_001077381.1. NM_001083912.1.
NP_001277471.1. NM_001290542.1.
NP_620091.2. NM_138752.2.
XP_006539504.1. XM_006539441.2.
XP_006539505.1. XM_006539442.2.
UniGeneiMm.235700.

3D structure databases

ProteinModelPortaliQ6KAU7.
SMRiQ6KAU7. Positions 100-406.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000092228.

PTM databases

iPTMnetiQ6KAU7.
PhosphoSiteiQ6KAU7.

Proteomic databases

EPDiQ6KAU7.
MaxQBiQ6KAU7.
PaxDbiQ6KAU7.
PRIDEiQ6KAU7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi101497.
KEGGimmu:101497.

Organism-specific databases

CTDi64857.
MGIiMGI:2141874. Plekhg2.

Phylogenomic databases

eggNOGiKOG3518. Eukaryota.
ENOG410XR81. LUCA.
HOGENOMiHOG000169126.
HOVERGENiHBG108256.
InParanoidiQ6KAU7.

Miscellaneous databases

ChiTaRSiPlekhg2. mouse.
PROiQ6KAU7.
SOURCEiSearch...

Gene expression databases

BgeeiQ6KAU7.

Family and domain databases

Gene3Di1.20.900.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR000219. DH-domain.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PfamiPF00169. PH. 1 hit.
PF00621. RhoGEF. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00325. RhoGEF. 1 hit.
[Graphical view]
SUPFAMiSSF48065. SSF48065. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50010. DH_2. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Activation of clg, a novel dbl family guanine nucleotide exchange factor gene, by proviral insertion at evi24, a common integration site in B cell and myeloid leukemias."
    Himmel K.L., Bi F., Shen H., Jenkins N.A., Copeland N.G., Zheng Y., Largaespada D.A.
    J. Biol. Chem. 277:13463-13472(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY, FUNCTION.
    Strain: BXH2.
  2. "Prediction of the coding sequences of mouse homologues of FLJ genes: the complete nucleotide sequences of 110 mouse FLJ-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Kitamura H., Nakagawa T., Nagase T., Ohara O., Koga H.
    DNA Res. 11:127-135(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Embryonic tail.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
    Strain: C57BL/6J and FVB/N.
    Tissue: Brain and Mammary tumor.
  4. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-912.

Entry informationi

Entry nameiPKHG2_MOUSE
AccessioniPrimary (citable) accession number: Q6KAU7
Secondary accession number(s): A0PJD9
, Q3UNL4, Q6PGK2, Q7TS89, Q8R4H6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: October 2, 2007
Last modified: June 8, 2016
This is version 82 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.