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Protein

Zinc finger protein 521

Gene

Znf521

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor that can both act as an activator or a repressor depending on the context. Involved in BMP signaling and in the regulation of the immature compartment of the hematopoietic system. Associates with SMADs in response to BMP2 leading to activate transcription of BMP target genes. Acts as a transcriptional repressor via its interaction with EBF1, a transcription factor involved specification of B-cell lineage; this interaction preventing EBF1 to bind DNA and activate target genes.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri47 – 6721C2H2-type 1; degeneratePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri118 – 14023C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri146 – 16823C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri174 – 19623C2H2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri202 – 22423C2H2-type 5PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri246 – 26924C2H2-type 6PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri281 – 30424C2H2-type 7PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri310 – 33223C2H2-type 8PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri405 – 42925C2H2-type 9; degeneratePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri437 – 46024C2H2-type 10PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri477 – 50024C2H2-type 11PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri513 – 53624C2H2-type 12PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri560 – 58526C2H2-type 13; atypicalPROSITE-ProRule annotationAdd
BLAST
Zinc fingeri634 – 65623C2H2-type 14PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri664 – 68623C2H2-type 15PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri694 – 71724C2H2-type 16PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri722 – 74524C2H2-type 17PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri752 – 77524C2H2-type 18PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri783 – 80523C2H2-type 19PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri809 – 83224C2H2-type 20PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri886 – 90823C2H2-type 21; degeneratePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri930 – 95223C2H2-type 22PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri959 – 98123C2H2-type 23PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri1020 – 104223C2H2-type 24PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri1065 – 108319C2H2-type 25; degeneratePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri1138 – 116124C2H2-type 26PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri1195 – 121723C2H2-type 27PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri1225 – 124723C2H2-type 28PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri1256 – 127924C2H2-type 29PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri1286 – 130924C2H2-type 30PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • neuron fate commitment Source: MGI
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein, Repressor

Keywords - Biological processi

Differentiation, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein 521
Alternative name(s):
Ecotropic viral integration site 3 protein
Gene namesi
Name:Znf521
Synonyms:Evi3, Zfp521
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:95459. Zfp521.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Involvement in diseasei

Defects in Znf521 are a cause of B-cell lymphomas. Znf521 gene is a frequent target of retroviral integration in murine B-cell lymphomas. Involved in most B-cell tumors in the AKXD-27 strain. Viral insertion causes overexpression resulting in the up-regulation of EBF1-target gene expression. Misexpression initiates tumorigenesis by perturbing B-cell development via an interaction with EBF1.

Keywords - Diseasei

Proto-oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13111311Zinc finger protein 521PRO_0000306872Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei546 – 5461PhosphoserineBy similarity
Modified residuei605 – 6051PhosphoserineCombined sources
Modified residuei608 – 6081PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ6KAS7.
PaxDbiQ6KAS7.
PeptideAtlasiQ6KAS7.
PRIDEiQ6KAS7.

PTM databases

iPTMnetiQ6KAS7.
PhosphoSiteiQ6KAS7.

Expressioni

Tissue specificityi

Widely expressed. Expressed in all B-cell stages.1 Publication

Gene expression databases

BgeeiENSMUSG00000024420.
CleanExiMM_ZFP521.
GenevisibleiQ6KAS7. MM.

Interactioni

Subunit structurei

Interacts with EBF1. Interacts with SMAD1 and SMAD4 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi230369. 4 interactions.
IntActiQ6KAS7. 1 interaction.
STRINGi10090.ENSMUSP00000025288.

Structurei

3D structure databases

ProteinModelPortaliQ6KAS7.
SMRiQ6KAS7. Positions 928-981.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

Uses different DNA- and protein-binding zinc fingers to regulate the distinct BMP-Smad and hematopoietic system.By similarity

Sequence similaritiesi

Contains 30 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri47 – 6721C2H2-type 1; degeneratePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri118 – 14023C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri146 – 16823C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri174 – 19623C2H2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri202 – 22423C2H2-type 5PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri246 – 26924C2H2-type 6PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri281 – 30424C2H2-type 7PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri310 – 33223C2H2-type 8PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri405 – 42925C2H2-type 9; degeneratePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri437 – 46024C2H2-type 10PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri477 – 50024C2H2-type 11PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri513 – 53624C2H2-type 12PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri560 – 58526C2H2-type 13; atypicalPROSITE-ProRule annotationAdd
BLAST
Zinc fingeri634 – 65623C2H2-type 14PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri664 – 68623C2H2-type 15PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri694 – 71724C2H2-type 16PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri722 – 74524C2H2-type 17PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri752 – 77524C2H2-type 18PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri783 – 80523C2H2-type 19PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri809 – 83224C2H2-type 20PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri886 – 90823C2H2-type 21; degeneratePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri930 – 95223C2H2-type 22PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri959 – 98123C2H2-type 23PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri1020 – 104223C2H2-type 24PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri1065 – 108319C2H2-type 25; degeneratePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri1138 – 116124C2H2-type 26PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri1195 – 121723C2H2-type 27PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri1225 – 124723C2H2-type 28PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri1256 – 127924C2H2-type 29PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri1286 – 130924C2H2-type 30PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00840000129874.
HOGENOMiHOG000155793.
HOVERGENiHBG052773.
InParanoidiQ6KAS7.
OMAiPAGEYIC.
OrthoDBiEOG091G00X5.
PhylomeDBiQ6KAS7.
TreeFamiTF331504.

Family and domain databases

Gene3Di3.30.160.60. 6 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF13912. zf-C2H2_6. 5 hits.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 30 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 27 hits.
PS50157. ZINC_FINGER_C2H2_2. 24 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6KAS7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSRRKQAKPR SLKDPNCKLE DKIEDGEAVD CKKRPEDGEE LEEDAVHSCD
60 70 80 90 100
SCLQVFESLS DITEHKIHQC QLTDGVDVED DPSCSWPASS PSSKDQTSPS
110 120 130 140 150
HGEGCDFGEE EGGPGLPYPC QFCDKSFSRL SYLKHHEQSH SDKLPFKCTY
160 170 180 190 200
CSRLFKHKRS RDRHIKLHTG DKKYHCSECD AAFSRSDHLK IHLKTHTSNK
210 220 230 240 250
PYKCAVCRRG FLSSSSLHGH MQVHERNKDG SQSGSRMEDW KMKDTQKCSQ
260 270 280 290 300
CEEGFDFPED LQKHIAECHP ECSPNEDRAA LQCMYCHELF VEETSLMNHI
310 320 330 340 350
EQVHGGEKKN SCSICSESFL TVEELYSHMD SHQQPESCNH SNSPSLVTVG
360 370 380 390 400
YTSVSSTTPD SNLSVDSSTM VEAAPPIPKS RGRKRAAQQT SDMTGPSSKQ
410 420 430 440 450
AKVTYSCIYC NKQLFSSLAV LQIHLKTMHL DKPEQAHICQ YCLEVLPSLY
460 470 480 490 500
NLNEHLKQVH EAQDPGLIVS AMPAIVYQCN FCSEVVNDLN TLQEHIRCSH
510 520 530 540 550
GFANPAAKDS NAFFCPHCYM GFLTDSSLEE HIRQVHCDLS GSRFGSPVLG
560 570 580 590 600
TPKEPVVEVY SCSYCTNSPI FNSVLKLNKH IKENHKNIPL ALNYIHNGKK
610 620 630 640 650
SRALSPLSPV AIEQTTLKMM QTVGGGPARA SGEYICNQCG AKYTSLDSFQ
660 670 680 690 700
THLKTHLDTV LPKLTCPQCN KEFPNQESLL KHVTIHFMIT STYYICESCD
710 720 730 740 750
KQFTSVDDLQ KHLLDMHTFV FFRCTLCQEV FDSKVSIQLH LAVKHSNEKK
760 770 780 790 800
VYRCTSCNWD FRNETDLQLH VKHNHLENQG KVHKCIFCGE SFGTEVELQC
810 820 830 840 850
HITTHSKKYN CRFCSKAFHA VILLEKHLRE KHCVFETKTP NCGTNGASEQ
860 870 880 890 900
VQKEEAELQT LLTNSQESHN SHDGSEEDVD SSEPMYGCDI CGAAYTMETL
910 920 930 940 950
LQNHQLRDHN IRPGESAIVK KKAELIKGNY KCNVCSRTFF SENGLREHMQ
960 970 980 990 1000
THLGPVKHYM CPICGERFPS LLTLTEHKVT HSKSLDTGNC RICKMPLQSE
1010 1020 1030 1040 1050
EEFLEHCQMH PDLRNSLTGF RCVVCMQTVT STLELKIHGT FHMQKTGNGS
1060 1070 1080 1090 1100
SVQTTGRGQH VQKLYKCASC LKEFRSKQDL VKLDINGLPY GLCAGCVNLS
1110 1120 1130 1140 1150
KSSSPGLSLP PGASRPGLGQ NESLSAMEGK GKAGGLKTRC SSCNVKFESE
1160 1170 1180 1190 1200
SELQNHIQTV HRELVPDANS TQLKTPQVSP MPRISPSQSD EKKTYQCIKC
1210 1220 1230 1240 1250
QMVFYNEWDI QVHVANHMID EGLNHECKLC SQTFDSPAKL QCHLIEHSFE
1260 1270 1280 1290 1300
GMGGTFKCPV CFTVFVQANK LQQHIFSAHG QEDKIYDCTQ CPQKFFFQTE
1310
LQNHTMTQHS S
Length:1,311
Mass (Da):147,666
Last modified:October 2, 2007 - v2
Checksum:iC8D922B5335C099A
GO
Isoform 2 (identifier: Q6KAS7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1191-1192: EK → G

Note: No experimental confirmation available.
Show »
Length:1,310
Mass (Da):147,466
Checksum:i9AAD063652AD7C5B
GO

Sequence cautioni

The sequence BAC36964 differs from that shown. Reason: Erroneous initiation. Curated
The sequence BAD21380 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti595 – 5951I → T in AAN39839 (PubMed:12393497).Curated
Sequence conflicti771 – 7711V → L in BAC38132 (PubMed:16141072).Curated
Sequence conflicti897 – 8971M → T in AAN39839 (PubMed:12393497).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1191 – 11922EK → G in isoform 2. 1 PublicationVSP_028553

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY147406 mRNA. Translation: AAN39839.1.
AK131130 mRNA. Translation: BAD21380.1. Different initiation.
BC021376 mRNA. Translation: AAH21376.1.
AK077697 mRNA. Translation: BAC36964.1. Different initiation.
AK081096 mRNA. Translation: BAC38132.1.
CCDSiCCDS37740.1. [Q6KAS7-1]
RefSeqiNP_663467.1. NM_145492.4. [Q6KAS7-1]
UniGeneiMm.40325.

Genome annotation databases

EnsembliENSMUST00000025288; ENSMUSP00000025288; ENSMUSG00000024420. [Q6KAS7-1]
GeneIDi225207.
KEGGimmu:225207.
UCSCiuc008edc.1. mouse. [Q6KAS7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY147406 mRNA. Translation: AAN39839.1.
AK131130 mRNA. Translation: BAD21380.1. Different initiation.
BC021376 mRNA. Translation: AAH21376.1.
AK077697 mRNA. Translation: BAC36964.1. Different initiation.
AK081096 mRNA. Translation: BAC38132.1.
CCDSiCCDS37740.1. [Q6KAS7-1]
RefSeqiNP_663467.1. NM_145492.4. [Q6KAS7-1]
UniGeneiMm.40325.

3D structure databases

ProteinModelPortaliQ6KAS7.
SMRiQ6KAS7. Positions 928-981.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi230369. 4 interactions.
IntActiQ6KAS7. 1 interaction.
STRINGi10090.ENSMUSP00000025288.

PTM databases

iPTMnetiQ6KAS7.
PhosphoSiteiQ6KAS7.

Proteomic databases

MaxQBiQ6KAS7.
PaxDbiQ6KAS7.
PeptideAtlasiQ6KAS7.
PRIDEiQ6KAS7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025288; ENSMUSP00000025288; ENSMUSG00000024420. [Q6KAS7-1]
GeneIDi225207.
KEGGimmu:225207.
UCSCiuc008edc.1. mouse. [Q6KAS7-1]

Organism-specific databases

CTDi225207.
MGIiMGI:95459. Zfp521.

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00840000129874.
HOGENOMiHOG000155793.
HOVERGENiHBG052773.
InParanoidiQ6KAS7.
OMAiPAGEYIC.
OrthoDBiEOG091G00X5.
PhylomeDBiQ6KAS7.
TreeFamiTF331504.

Miscellaneous databases

PROiQ6KAS7.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024420.
CleanExiMM_ZFP521.
GenevisibleiQ6KAS7. MM.

Family and domain databases

Gene3Di3.30.160.60. 6 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF13912. zf-C2H2_6. 5 hits.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 30 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 27 hits.
PS50157. ZINC_FINGER_C2H2_2. 24 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiZN521_MOUSE
AccessioniPrimary (citable) accession number: Q6KAS7
Secondary accession number(s): Q8BIF5
, Q8BV21, Q8CIQ2, Q8VDS6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: October 2, 2007
Last modified: September 7, 2016
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.