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Q6KAI0

- PNP2_ORYSJ

UniProt

Q6KAI0 - PNP2_ORYSJ

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Protein

Polyribonucleotide nucleotidyltransferase 2, mitochondrial

Gene

PNP2

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Involved in the 3'-end maturation of mitochondrial mRNAs, rRNAs and tRNAs. Functions as a poly(A) mRNA 3'-5' degrading phosphorylase (By similarity).By similarity

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: EnsemblPlants/Gramene
  2. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-EC
  3. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. mitochondrial RNA catabolic process Source: EnsemblPlants/Gramene
  2. mitochondrial RNA processing Source: EnsemblPlants/Gramene
  3. mRNA catabolic process Source: EnsemblPlants/Gramene
  4. mRNA processing Source: UniProtKB-KW
  5. rRNA processing Source: UniProtKB-KW
  6. transcription factor import into nucleus Source: EnsemblPlants/Gramene
  7. tRNA processing Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Exonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase

Keywords - Biological processi

mRNA processing, rRNA processing, tRNA processing

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferase 2, mitochondrial (EC:2.7.7.8)
Alternative name(s):
Polynucleotide phosphorylase 2
Short name:
PNPase 2
Gene namesi
Name:PNP2
Ordered Locus Names:Os02g0617700, LOC_Os02g40460
ORF Names:OJ1014_H03.14
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763: Chromosome 2

Organism-specific databases

GrameneiQ6KAI0.

Subcellular locationi

Mitochondrion By similarity

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3939MitochondrionSequence AnalysisAdd
BLAST
Chaini40 – 982943Polyribonucleotide nucleotidyltransferase 2, mitochondrialPRO_0000420277Add
BLAST

Expressioni

Gene expression databases

ExpressionAtlasiQ6KAI0. baseline.

Structurei

3D structure databases

ProteinModelPortaliQ6KAI0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini624 – 67855KHAdd
BLAST
Domaini689 – 75769S1 motif 1Add
BLAST
Domaini920 – 98263S1 motif 2Add
BLAST

Sequence similaritiesi

Contains 1 KH domain.Curated
Contains 2 S1 motif domains.Curated

Keywords - Domaini

Repeat, Transit peptide

Phylogenomic databases

InParanoidiQ6KAI0.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
SMARTiSM00316. S1. 2 hits.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 2 hits.
SSF54211. SSF54211. 2 hits.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6KAI0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSMAVASLRL LARGGRRRAR FPAPLSVPGG RAAFLSGAAE EVAQADAPPP
60 70 80 90 100
PPPGRKVLES FREEFEIGGR VISFETGKMA RFANGSVVIS MDDTHVLSTV
110 120 130 140 150
AAAKSSEPVR DFLPLTVDYQ EKQYAQGVIP TTYMRREGAP KERELLCGRI
160 170 180 190 200
IDRPIRPLFP PGFYHEVQVM NATIIMVNVI SSDGKQDPDV MAANASSAAL
210 220 230 240 250
MLSDIPWNGP IGVIRVGRID GNFVLNPTVD ELGLSDLNLV YACSRDKTLM
260 270 280 290 300
IDVQAREITE RDLQAGMKLA HAEAVKCINP QLRLAKRAGK KKKEYKISLI
310 320 330 340 350
SDKSYEKIRT LSEAPIEEVF TDSTYGKFER GEALENITQS VKAKLEEECD
360 370 380 390 400
EDSLKFLHKA VDTVRKQVIR KRIIEKGLRV DGRQLDEVRP LYCESSTYPI
410 420 430 440 450
LHGSALFSRG DTQVLCTVTL GAPGDAQRLD SIVGPPTKRF MLHYSFPPFS
460 470 480 490 500
INEVAKRGGL NRREVGHGTL AEKALLAVLP PEGEFPYTVR VNSEVMASDG
510 520 530 540 550
STSMASVCGG SMALMDAGIP VREHVAGVSV GLVSEVDQTT GDISSYRILT
560 570 580 590 600
DILGLEDHLG DMDFKIAGTR RGITAIQLDI KPAGIPLDII CESLEPARKA
610 620 630 640 650
RNQILDRMDQ EISSARAFND GSSPRLATLS FSSDSLRKLL FHRKKIEQET
660 670 680 690 700
GARVSVSDGT VTIVAKTQPI MDKAIEKVEF LVGREIEVGR TYKGVVSSIK
710 720 730 740 750
EYGAFVEFNG GQQGLLHISE LSHDKVSKVS DVVSVGQVLS LTCIGQDLRG
760 770 780 790 800
NIKLSLKATL PHAHEKKDLA SNHTDPLPSQ EVVGWTAVEN MPSKDANAEP
810 820 830 840 850
SISKDEDNMI EETPGCSTPA VIIRSAAECD AQDVTNDPKK KRPKVAKSSP
860 870 880 890 900
KLSKPASERQ EVKRTSAKKT SGASTTAKKN KKEKADSSND VLDAIPEQNK
910 920 930 940 950
SNIMNYSSPS NFRSGSMKLG DVVTAKVYQI RAYGLVLELS DGVRGMHKFA
960 970 980
ENGHKDFEVG EELLVKCSSF NAKGIPVFSL LD
Length:982
Mass (Da):107,028
Last modified:July 5, 2004 - v1
Checksum:i0B05E5A0E8684351
GO

Sequence cautioni

The sequence BAF09358.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP003980 Genomic DNA. Translation: BAD21450.1.
AP008208 Genomic DNA. Translation: BAF09358.1. Sequence problems.
AK058997 mRNA. No translation available.
RefSeqiNP_001047444.1. NM_001053979.1.
UniGeneiOs.37362.

Genome annotation databases

GeneIDi4329995.
KEGGiosa:4329995.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP003980 Genomic DNA. Translation: BAD21450.1 .
AP008208 Genomic DNA. Translation: BAF09358.1 . Sequence problems.
AK058997 mRNA. No translation available.
RefSeqi NP_001047444.1. NM_001053979.1.
UniGenei Os.37362.

3D structure databases

ProteinModelPortali Q6KAI0.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 4329995.
KEGGi osa:4329995.

Organism-specific databases

Gramenei Q6KAI0.

Phylogenomic databases

InParanoidi Q6KAI0.

Gene expression databases

ExpressionAtlasi Q6KAI0. baseline.

Family and domain databases

Gene3Di 1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPi MF_01595. PNPase.
InterProi IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view ]
PANTHERi PTHR11252. PTHR11252. 1 hit.
Pfami PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view ]
SMARTi SM00316. S1. 2 hits.
[Graphical view ]
SUPFAMi SSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 2 hits.
SSF54211. SSF54211. 2 hits.
SSF55666. SSF55666. 2 hits.
TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
PROSITEi PS50126. S1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  2. "The rice annotation project database (RAP-DB): 2008 update."
    The rice annotation project (RAP)
    Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  3. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
    The rice full-length cDNA consortium
    Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 661-982.
    Strain: cv. Nipponbare.

Entry informationi

Entry nameiPNP2_ORYSJ
AccessioniPrimary (citable) accession number: Q6KAI0
Secondary accession number(s): Q0DZI0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2012
Last sequence update: July 5, 2004
Last modified: October 29, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3