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Q6KAI0

- PNP2_ORYSJ

UniProt

Q6KAI0 - PNP2_ORYSJ

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Protein
Polyribonucleotide nucleotidyltransferase 2, mitochondrial
Gene
PNP2, Os02g0617700, LOC_Os02g40460, OJ1014_H03.14
Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Involved in the 3'-end maturation of mitochondrial mRNAs, rRNAs and tRNAs. Functions as a poly(A) mRNA 3'-5' degrading phosphorylase By similarity.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. RNA binding Source: UniProtKB-KW
  3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. mRNA catabolic process Source: InterPro
  2. mRNA processing Source: UniProtKB-KW
  3. mitochondrial RNA catabolic process Source: EnsemblPlants/Gramene
  4. mitochondrial RNA processing Source: EnsemblPlants/Gramene
  5. rRNA processing Source: UniProtKB-KW
  6. tRNA processing Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Exonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase

Keywords - Biological processi

mRNA processing, rRNA processing, tRNA processing

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferase 2, mitochondrial (EC:2.7.7.8)
Alternative name(s):
Polynucleotide phosphorylase 2
Short name:
PNPase 2
Gene namesi
Name:PNP2
Ordered Locus Names:Os02g0617700, LOC_Os02g40460
ORF Names:OJ1014_H03.14
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763: Chromosome 2

Organism-specific databases

GrameneiQ6KAI0.

Subcellular locationi

Mitochondrion By similarity UniRule annotation

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3939Mitochondrion Reviewed prediction
Add
BLAST
Chaini40 – 982943Polyribonucleotide nucleotidyltransferase 2, mitochondrialUniRule annotation
PRO_0000420277Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ6KAI0.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini624 – 67855KH
Add
BLAST
Domaini689 – 75769S1 motif 1
Add
BLAST
Domaini920 – 98263S1 motif 2
Add
BLAST

Sequence similaritiesi

Contains 1 KH domain.
Contains 2 S1 motif domains.

Keywords - Domaini

Repeat, Transit peptide

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
SMARTiSM00316. S1. 2 hits.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 2 hits.
SSF54211. SSF54211. 2 hits.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6KAI0-1 [UniParc]FASTAAdd to Basket

« Hide

MSMAVASLRL LARGGRRRAR FPAPLSVPGG RAAFLSGAAE EVAQADAPPP    50
PPPGRKVLES FREEFEIGGR VISFETGKMA RFANGSVVIS MDDTHVLSTV 100
AAAKSSEPVR DFLPLTVDYQ EKQYAQGVIP TTYMRREGAP KERELLCGRI 150
IDRPIRPLFP PGFYHEVQVM NATIIMVNVI SSDGKQDPDV MAANASSAAL 200
MLSDIPWNGP IGVIRVGRID GNFVLNPTVD ELGLSDLNLV YACSRDKTLM 250
IDVQAREITE RDLQAGMKLA HAEAVKCINP QLRLAKRAGK KKKEYKISLI 300
SDKSYEKIRT LSEAPIEEVF TDSTYGKFER GEALENITQS VKAKLEEECD 350
EDSLKFLHKA VDTVRKQVIR KRIIEKGLRV DGRQLDEVRP LYCESSTYPI 400
LHGSALFSRG DTQVLCTVTL GAPGDAQRLD SIVGPPTKRF MLHYSFPPFS 450
INEVAKRGGL NRREVGHGTL AEKALLAVLP PEGEFPYTVR VNSEVMASDG 500
STSMASVCGG SMALMDAGIP VREHVAGVSV GLVSEVDQTT GDISSYRILT 550
DILGLEDHLG DMDFKIAGTR RGITAIQLDI KPAGIPLDII CESLEPARKA 600
RNQILDRMDQ EISSARAFND GSSPRLATLS FSSDSLRKLL FHRKKIEQET 650
GARVSVSDGT VTIVAKTQPI MDKAIEKVEF LVGREIEVGR TYKGVVSSIK 700
EYGAFVEFNG GQQGLLHISE LSHDKVSKVS DVVSVGQVLS LTCIGQDLRG 750
NIKLSLKATL PHAHEKKDLA SNHTDPLPSQ EVVGWTAVEN MPSKDANAEP 800
SISKDEDNMI EETPGCSTPA VIIRSAAECD AQDVTNDPKK KRPKVAKSSP 850
KLSKPASERQ EVKRTSAKKT SGASTTAKKN KKEKADSSND VLDAIPEQNK 900
SNIMNYSSPS NFRSGSMKLG DVVTAKVYQI RAYGLVLELS DGVRGMHKFA 950
ENGHKDFEVG EELLVKCSSF NAKGIPVFSL LD 982
Length:982
Mass (Da):107,028
Last modified:July 5, 2004 - v1
Checksum:i0B05E5A0E8684351
GO

Sequence cautioni

The sequence BAF09358.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP003980 Genomic DNA. Translation: BAD21450.1.
AP008208 Genomic DNA. Translation: BAF09358.1. Sequence problems.
AK058997 mRNA. No translation available.
RefSeqiNP_001047444.1. NM_001053979.1.
UniGeneiOs.37362.

Genome annotation databases

GeneIDi4329995.
KEGGiosa:4329995.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP003980 Genomic DNA. Translation: BAD21450.1 .
AP008208 Genomic DNA. Translation: BAF09358.1 . Sequence problems.
AK058997 mRNA. No translation available.
RefSeqi NP_001047444.1. NM_001053979.1.
UniGenei Os.37362.

3D structure databases

ProteinModelPortali Q6KAI0.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 4329995.
KEGGi osa:4329995.

Organism-specific databases

Gramenei Q6KAI0.

Family and domain databases

Gene3Di 1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPi MF_01595. PNPase.
InterProi IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view ]
PANTHERi PTHR11252. PTHR11252. 1 hit.
Pfami PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view ]
SMARTi SM00316. S1. 2 hits.
[Graphical view ]
SUPFAMi SSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 2 hits.
SSF54211. SSF54211. 2 hits.
SSF55666. SSF55666. 2 hits.
TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
PROSITEi PS50126. S1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  2. "The rice annotation project database (RAP-DB): 2008 update."
    The rice annotation project (RAP)
    Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  3. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
    The rice full-length cDNA consortium
    Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 661-982.
    Strain: cv. Nipponbare.

Entry informationi

Entry nameiPNP2_ORYSJ
AccessioniPrimary (citable) accession number: Q6KAI0
Secondary accession number(s): Q0DZI0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2012
Last sequence update: July 5, 2004
Last modified: April 16, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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