Reviewed, UniProtKB/Swiss-Prot Q6K833 (MCES2_ORYSJ)

Names and origin

Protein names

Recommended name:
    mRNA cap guanine-N7 methyltransferase 2
    EC=2.1.1.56
Alternative name(s):
    mRNA (guanine-N(7)-)-methyltransferase 2
    mRNA cap methyltransferase 2

Gene names

Ordered Locus Names:	Os02g0780600, LOC_Os02g54000
ORF Names:	OJ1369_G08.2

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	339 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	mRNA-capping methyltransferase that methylates the N7 position of the added guanosine to the 5'-cap structure of mRNAs. Binds RNA containing 5'-terminal GpppC By similarity.
Catalytic activity	S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m(7)G(5')pppR-RNA.
Subcellular location	NucleusBy similarity.
Sequence similarities	Belongs to the mRNA cap methyltransferase family.

Ontologies

Keywords
Biological process	mRNA capping mRNA processing
Cellular component	Nucleus
Ligand	RNA-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	mRNA capping Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW mRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 339	339	mRNA cap guanine-N7 methyltransferase 2		PRO_0000260153
Sites
	Binding site	14	1	S-adenosyl-L-methionine By similarity
	Binding site	41	1	mRNA cap By similarity
	Binding site	54	1	S-adenosyl-L-methionine By similarity
	Binding site	269	1	mRNA cap By similarity
Experimental info
	Sequence conflict	116	1	E → V in AK121526. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q6K833-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 136DA11E7D0807CB	FASTA	339	38,388
	10 20 30 40 50 60 MAVTPHHRLY EFAKTALIKI FAFPYATVCD LYCDGGVDTD KWGDAQIGHY IGIDASASGV 70 80 90 100 110 120 NDARELWESR KKLFTSEFIE LDPSADDFEA QMQEKGIQAD IVCCMQHLQL CFESEEHAQK 130 140 150 160 170 180 LLNNVSSLLK PGGYFVGIIP DSSTIWTKYQ KNVEASHNKG LKTVPNSIRS ENYVITFEVE 190 200 210 220 230 240 EEKFPFFGKK YQLKFANESM FENHCLVHFP SFMRLAREAG LEYVEIQNLT EFYDDNRTQF 250 260 270 280 290 300 APLLGGYGSS LVDPRGKLVA RSFDILGLYS TFVFQKPDPD AMPPIVTPEL HDPENDQEEE 310 320 330 WLWTQQASMD DGRVSRTDIL PPADNEKGIL GPGPADMRL

References

[1]	"Oryza sativa nipponbare chromosome 2 genomic DNA sequence." The international rice genome sequencing project (IRGSP) consortium Submitted (OCT-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP004257 Genomic DNA. Translation: BAD19434.1. AP008208 Genomic DNA. Translation: BAF10219.1. AK067608 mRNA. No translation available. AK121526 mRNA. No translation available.
RefSeq	NP_001048305.1.
UniGene	Os.10219
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4330921.
KEGG	osa:4330921.
Organism-specific databases
Gramene	Q6K833.
Family and domain databases
InterPro	IPR013217. Methyltransf_12. [Graphical view]
Pfam	PF08242. Methyltransf_12. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MCES2_ORYSJ

Accession

Primary (citable) accession number: Q6K833

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	July 5, 2004
Last modified:	November 4, 2008
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)