Q6K7G9 (GUN8_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endoglucanase 8
EC=3.2.1.4

Alternative name(s):
Endo-1,4-beta glucanase 8

Gene names

Ordered Locus Names:	Os02g0778600, LOC_Os02g53820
ORF Names:	OJ1293_A01.6, OsJ_008299

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza › Oryza sativa

Protein attributes

Sequence length	501 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 9 (cellulase E) family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cell wall biogenesis/degradation Cellulose degradation Polysaccharide degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 35	35	Potential
	Chain	36 – 501	466	Endoglucanase 8		PRO_0000249285
Sites
	Active site	414	1	By similarity
	Active site	465	1	By similarity
	Active site	474	1	By similarity
Amino acid modifications
	Glycosylation	298	1	N-linked (GlcNAc...) Potential
	Glycosylation	462	1	N-linked (GlcNAc...) Potential
	Glycosylation	469	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q6K7G9 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 196C62B858CFCC08	FASTA	501	54,541
	10 20 30 40 50 60 MKPRSSRDGH NAAAAAALLL AALVLSGDVL PAVVAGGAPS FNYKDALTKS IMFLEAQRSG 70 80 90 100 110 120 KLPPTNRIKW RGDSGMEDGK LANVDLTGGY YDAGDNVKYG LPLAFTVTTL AWTAMAFEKE 130 140 150 160 170 180 LKAARELENV HAAIRWGTDY FLKAATKKDH LWVQVGDPNA DHQCWVRPEN MPTPRTLYQI 190 200 210 220 230 240 NDKTPGSEIA AETAAAMTAS SMVFRKDKPY SRRLLNKAKL LFQFAKTHQG TYDGECPFYC 250 260 270 280 290 300 SYSGYNDELL WAATWLYLAT KRQVYADFIG HEAISSSVAE FSWDLKFPGA QVLLAELNMT 310 320 330 340 350 360 SSGGLQSFKS QADNFVCAVL PDTPFHQVSI TPGGMIHLRD GANSQYVTST AFLFVAYSDI 370 380 390 400 410 420 LRRINQPVMC GAQAVQPARL LQFAKQQIDY LLGANPRGRS YVVGFGVNPP TQPHHRGAST 430 440 450 460 470 480 PVLPPGYQVN CGMSFSEWFT PDRPNPNELT GAIMGGPDGG DNFSDKRGNS SCTEPCTYIN 490 500 SLSIGPLAAL AIRGPNLIAT Q

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[3]	"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[4]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP004846 Genomic DNA. Translation: BAD19513.1. AP008208 Genomic DNA. Translation: BAF10211.1. CM000139 Genomic DNA. Translation: EAZ24816.1. AK100190 mRNA. Translation: BAG94487.1. AK106971 mRNA. Translation: BAG97900.1.
RefSeq	NP_001048297.1. NM_001054832.1.
UniGene	Os.53793.
3D structure databases
HSSP	HSSP built from PDB template 1TF4 based on UniProtKB P26221.
ProteinModelPortal	Q6K7G9.
ModBase	Search...
Protein family/group databases
CAZy	GH9. Glycoside Hydrolase Family 9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os02g53820.2; LOC_Os02g53820.2; LOC_Os02g53820.
GeneID	4330912.
KEGG	osa:4330912.
Organism-specific databases
Gramene	Q6K7G9.
Phylogenomic databases
eggNOG	NOG317757.
HOGENOM	HOG000021033.
KO	K01179.
ProtClustDB	PLN02909.
Family and domain databases
Gene3D	1.50.10.10. 1 hit.
InterPro	IPR008928. 6-hairpin_glycosidase-like. IPR012341. 6hp_glycosidase. IPR001701. Glyco_hydro_9. IPR018221. Glyco_hydro_9_AS. [Graphical view]
Pfam	PF00759. Glyco_hydro_9. 1 hit. [Graphical view]
SUPFAM	SSF48208. Glyco_trans_6hp. 1 hit.
PROSITE	PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit. PS00698. GLYCOSYL_HYDROL_F9_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUN8_ORYSJ

Accession

Primary (citable) accession number: Q6K7G9
Secondary accession number(s): Q0DX27

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	July 5, 2004
Last modified:	April 3, 2013
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program