Reviewed, UniProtKB/Swiss-Prot Q6K669 (AMPL2_ORYSJ)

Names and origin

Protein names

Recommended name:
    Leucine aminopeptidase 2, chloroplastic
    EC=3.4.11.1
Alternative name(s):
    Leucyl aminopeptidase 2
      Short name=LAP 2
    Proline aminopeptidase 2
    EC=3.4.11.5
    Prolyl aminopeptidase 2

Gene names

Ordered Locus Names:	Os02g0794700, LOC_Os02g55140
ORF Names:	OJ1695_H09.4, P0700F06.36

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	598 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides By similarity.
Catalytic activity	Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. Release of N-terminal proline from a peptide.
Cofactor	Binds 2 zinc ions per subunit By similarity.
Subunit structure	Homohexamer Probable.
Subcellular location	Plastid › chloroplast Potential.
Sequence similarities	Belongs to the peptidase M17 family.

Ontologies

Keywords
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	Metal-binding Zinc
Molecular function	Aminopeptidase Hydrolase Protease
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: InterPro metalloexopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 71	71	Chloroplast Potential
	Chain	72 – 598	527	Leucine aminopeptidase 2, chloroplastic		PRO_0000247503
Sites
	Active site	379	1	Potential
	Active site	456	1	Potential
	Metal binding	367	1	Zinc 2 By similarity
	Metal binding	372	1	Zinc 1 By similarity
	Metal binding	372	1	Zinc 2 By similarity
	Metal binding	392	1	Zinc 2 By similarity
	Metal binding	452	1	Zinc 1 By similarity
	Metal binding	454	1	Zinc 1 By similarity
	Metal binding	454	1	Zinc 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6K669-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 29E18595E0A22F69	FASTA	598	61,819
	10 20 30 40 50 60 MATAASTSAA AVAAASRLLV RRAPPRLLRR LPRAALAASR PSPPSSSSYG AAAVALGRQP 70 80 90 100 110 120 LGHRARMGHT AAAAAAAGPA LGLTKPNAVE PPQVSFAAKD VEFSEWKGDI LAIAVTENDL 130 140 150 160 170 180 VKGSDSKFEN AVLKKLDGQL GGLLSEASAE EDFTGKAGQS VVLRLPGQGF KRVGLIGLGQ 190 200 210 220 230 240 NAPSTTTACK GIGESVASVA KSAQASSAAI VFASVGGIQE DFKLTAAAAI ASGTVLGLHE 250 260 270 280 290 300 DSRYKSESKK VHLKQVDLIG FGSGPEVDQK LKYANDLSSG VIFGKELVNS PANVLTPAVL 310 320 330 340 350 360 AEEASNIAST YSDVFTATIL DVEKCKELKM GSYLGVAAAS ANPPHFIHLC YKPPGGNAKR 370 380 390 400 410 420 KLAIVGKGLT FDSGGYNIKT GPGCSIELMK FDMGGSAAVF GAAKALGQIK PPGVEVHFIV 430 440 450 460 470 480 AACENMISGT GMRPGDIVTA SNGKTIEVNN TDAEGRLTLA DALVYACNQG VDKIIDLATL 490 500 510 520 530 540 TGACVVALGP SIAGIFTPSD ELAKEVAAAS EISGEKFWRM PLEESYWESM KSGVADMVNT 550 560 570 580 590 GGRQGGSITA ALFLKQFVDE KVQWMHIDMA GPVWNDKKRA ATGFGVSTLV EWVLKNSS

References

[1]	"Oryza sativa nipponbare chromosome 2 genomic DNA sequence." The international rice genome sequencing project (IRGSP) consortium Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 283-598. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP004094 Genomic DNA. Translation: BAD19264.1. AP005115 Genomic DNA. Translation: BAD19673.1. AK058513 mRNA. No translation available.
3D structure databases
SMR	Q6K669. Positions 109-597.
ModBase	Search...
Protein family/group databases
MEROPS	M17.002.
Organism-specific databases
Gramene	Q6K669.
Family and domain databases
InterPro	IPR011356. Peptidase_M17. IPR000819. Peptidase_M17_C. IPR008283. Peptidase_M17_N. [Graphical view]
PANTHER	PTHR11963:SF3. Peptidase_M17. 1 hit.
Pfam	PF00883. Peptidase_M17. 1 hit. PF02789. Peptidase_M17_N. 1 hit. [Graphical view]
PRINTS	PR00481. LAMNOPPTDASE.
PROSITE	PS00631. CYTOSOL_AP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPL2_ORYSJ

Accession

Primary (citable) accession number: Q6K669

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	July 5, 2004
Last modified:	January 19, 2010
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)