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Protein

A-kinase anchor protein 7 isoforms delta and gamma

Gene

Akap7

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probably targets cAMP-dependent protein kinase (PKA) to the cellular membrane or cytoskeletal structures. The membrane-associated form reduces epithelial sodium channel (ENaC) activity, whereas the free cytoplasmic form may negatively regulate ENaC channel feedback inhibition by intracellular sodium (By similarity). Isoform Delta may be involved in shuttling aquaporin-2 (AQP2) to the plasma membrane.By similarityCurated1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei134 – 1341AMP1 Publication
Binding sitei134 – 1341CMP1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi224 – 2263AMP1 Publication
Nucleotide bindingi224 – 2263CMP1 Publication

GO - Molecular functioni

  • AMP binding Source: RGD
  • protein complex scaffold Source: RGD
  • protein C-terminus binding Source: RGD
  • protein domain specific binding Source: RGD
  • protein kinase A binding Source: RGD
  • protein kinase A regulatory subunit binding Source: RGD

GO - Biological processi

  • protein localization Source: RGD
  • regulation of protein kinase A signaling Source: RGD
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
A-kinase anchor protein 7 isoforms delta and gamma
Short name:
AKAP-7 isoforms delta and gamma
Alternative name(s):
A-kinase anchor protein 18By similarity
AKAP-18By similarity
Protein kinase A-anchoring protein 7 isoforms delta and gamma
Short name:
PRKA7 isoforms delta and gamma
Gene namesi
Name:Akap7Imported
Synonyms:Akap181 Publication
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1303071. Akap7.

Subcellular locationi

Isoform Gamma :
Isoform Delta :

GO - Cellular componenti

  • apical plasma membrane Source: RGD
  • cytosol Source: RGD
  • exocytic vesicle Source: RGD
  • membrane Source: RGD
  • nucleus Source: UniProtKB-SubCell
  • protein complex Source: RGD
  • sarcoplasmic reticulum Source: RGD
  • T-tubule Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi308 – 3081L → P: Reduces PKA-RII-alpha binding. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 353353A-kinase anchor protein 7 isoforms delta and gammaPRO_0000419635Add
BLAST

Proteomic databases

PaxDbiQ6JP77.
PRIDEiQ6JP77.

Expressioni

Tissue specificityi

Expressed highly in the heart, and moderately in brain, lung, liver, kidney and testis. Hardly detectable in spleen and skeletal muscle. In kidney, isoform Delta is expressed in the principal cells of the IMCD.1 Publication

Interactioni

Subunit structurei

Binds cAMP-dependent protein kinase (PKA). Interacts with PRKCA; only the cytoplasmic form is capable of interacting with PRKCA.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PlnP610166EBI-6096191,EBI-7620725

GO - Molecular functioni

  • protein complex scaffold Source: RGD
  • protein C-terminus binding Source: RGD
  • protein domain specific binding Source: RGD
  • protein kinase A binding Source: RGD
  • protein kinase A regulatory subunit binding Source: RGD

Protein-protein interaction databases

BioGridi262695. 1 interaction.
IntActiQ6JP77. 1 interaction.
MINTiMINT-5227061.
STRINGi10116.ENSRNOP00000017617.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2VFKX-ray1.50A88-292[»]
2VFLX-ray2.25A88-292[»]
2VFYX-ray1.80A88-292[»]
3J4Qelectron microscopy35.00A1-353[»]
3J4Relectron microscopy35.00A1-353[»]
ProteinModelPortaliQ6JP77.
SMRiQ6JP77. Positions 88-292.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni299 – 35355PKA-RII-alpha subunit binding domain1 PublicationAdd
BLAST
Regioni300 – 32425RI-alpha-bindingBy similarityAdd
BLAST
Regioni301 – 31414RII-bindingBy similarityAdd
BLAST

Phylogenomic databases

eggNOGiKOG2814. Eukaryota.
ENOG4111FMP. LUCA.
HOGENOMiHOG000294077.
HOVERGENiHBG050475.
InParanoidiQ6JP77.
KOiK16524.
PhylomeDBiQ6JP77.
TreeFamiTF105406.

Family and domain databases

Gene3Di3.90.1140.10. 1 hit.
InterProiIPR019511. AKAP7_RI-RII-bd_dom.
IPR019510. Kinase-A_anchor_nucl_local_sig.
IPR009097. RNA_ligase/cNuc_Pdiesterase.
[Graphical view]
PfamiPF10469. AKAP7_NLS. 1 hit.
PF10470. AKAP7_RIRII_bdg. 1 hit.
[Graphical view]
SUPFAMiSSF55144. SSF55144. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Delta1 Publication (identifier: Q6JP77-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MERPAAGEID ANKCDHLSRG EEGTGDLETS PVGSLADLPF AAVDIQDDCG
60 70 80 90 100
LPDVPQGNVP QGNPKRSKEN RGDRNDHVKK RKKAKKDYQP NYFLSIPITN
110 120 130 140 150
KKITAGIKVL QNSILRQDNR LTKAMVGDGS FHITLLVMQL LNEDEVNIGT
160 170 180 190 200
DALLELKPFV EEILEGKHLT LPFHGIGTFQ GQVGFVKLAD GDHVSALLEI
210 220 230 240 250
AETAKRTFQE KGILAGESRT FKPHLTFMKL SKAPMLWKKG VRKIEPGLYE
260 270 280 290 300
QFIDHRFGEE ILYQIDLCSM LKKKQSNGYY HCESSIVIGE KDRKEPEDAE
310 320 330 340 350
LVRLSKRLVE NAVLKAVQQY LEETQNKKQP GEGNSVKAEE GDRNGDGSDN

NRK
Length:353
Mass (Da):39,418
Last modified:July 5, 2004 - v1
Checksum:i5C1712A9B04E6CF3
GO
Isoform Gamma1 Publication (identifier: Q6JP77-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     56-60: Missing.

Show »
Length:348
Mass (Da):38,922
Checksum:i923D9C104B48FDC3
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei56 – 605Missing in isoform Gamma. 1 PublicationVSP_044297

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY350741 mRNA. Translation: AAR06859.1.
BC098632 mRNA. Translation: AAH98632.1.
RefSeqiNP_001001801.1. NM_001001801.3. [Q6JP77-1]
UniGeneiRn.204773.

Genome annotation databases

GeneIDi361458.
KEGGirno:361458.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY350741 mRNA. Translation: AAR06859.1.
BC098632 mRNA. Translation: AAH98632.1.
RefSeqiNP_001001801.1. NM_001001801.3. [Q6JP77-1]
UniGeneiRn.204773.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2VFKX-ray1.50A88-292[»]
2VFLX-ray2.25A88-292[»]
2VFYX-ray1.80A88-292[»]
3J4Qelectron microscopy35.00A1-353[»]
3J4Relectron microscopy35.00A1-353[»]
ProteinModelPortaliQ6JP77.
SMRiQ6JP77. Positions 88-292.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi262695. 1 interaction.
IntActiQ6JP77. 1 interaction.
MINTiMINT-5227061.
STRINGi10116.ENSRNOP00000017617.

Proteomic databases

PaxDbiQ6JP77.
PRIDEiQ6JP77.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi361458.
KEGGirno:361458.

Organism-specific databases

CTDi9465.
RGDi1303071. Akap7.

Phylogenomic databases

eggNOGiKOG2814. Eukaryota.
ENOG4111FMP. LUCA.
HOGENOMiHOG000294077.
HOVERGENiHBG050475.
InParanoidiQ6JP77.
KOiK16524.
PhylomeDBiQ6JP77.
TreeFamiTF105406.

Miscellaneous databases

PROiQ6JP77.

Family and domain databases

Gene3Di3.90.1140.10. 1 hit.
InterProiIPR019511. AKAP7_RI-RII-bd_dom.
IPR019510. Kinase-A_anchor_nucl_local_sig.
IPR009097. RNA_ligase/cNuc_Pdiesterase.
[Graphical view]
PfamiPF10469. AKAP7_NLS. 1 hit.
PF10470. AKAP7_RIRII_bdg. 1 hit.
[Graphical view]
SUPFAMiSSF55144. SSF55144. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAKA7G_RAT
AccessioniPrimary (citable) accession number: Q6JP77
Secondary accession number(s): D3ZMQ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2012
Last sequence update: July 5, 2004
Last modified: September 7, 2016
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.