Reviewed,
UniProtKB/Swiss-Prot Q6JHU8 (P3H1_CHICK)
Last modified
December 16, 2008.
Version 36.
History...
Clusters with 100%,
90%,
50% identity |
Documents (1) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Prolyl 3-hydroxylase 1 EC=1.14.11.7 Alternative name(s): Leucine- and proline-enriched proteoglycan 1 homolog Short name=Leprecan-1 homolog | ||||
| Gene names |
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| Organism | Gallus gallus (Chicken) | ||||
| Taxonomic identifier | 9031 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus |
Protein attributes
| Sequence length | 725 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Has prolyl 3-hydroxylase activity catalyzing the post-translational formation of 3-hydroxyproline in -Xaa-Pro-Gly-sequences in collagens, especially types IV and V. May be involved in the secretoty pathway of cells. Has growth suppressive activity in fibroblasts By similarity. |
| Catalytic activity | Procollagen L-proline + 2-oxoglutarate + O(2) = procollagen trans-3-hydroxy-L-proline + succinate + CO(2). |
| Cofactor | Iron By similarity. Ascorbate By similarity. |
| Subunit structure | Binds unfolded collagen in a complex with CYPB and CRTAP. |
| Subcellular location | Endoplasmic reticulumBy similarity. |
| Tissue specificity | Expressed in embryonic dermis, tendon, cartilage, liver and kidney. Expression in the kidney is restricted to the calyx. In the liver, expression is restricted to the interlobular septum. Ref.1 |
| Sequence similarities | Belongs to the leprecan family. Contains 1 PKHD (prolyl/lysyl hydroxylase) domain. Contains 4 TPR repeats. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 14 | 14 | Potential | ||||||
| Chain | 15 – 725 | 711 | Prolyl 3-hydroxylase 1 | PRO_0000240355 | |||||
Regions | |||||||||
| Repeat | 36 – 69 | 34 | TPR 1 | ||||||
| Repeat | 136 – 169 | 34 | TPR 2 | ||||||
| Repeat | 198 – 231 | 34 | TPR 3 | ||||||
| Repeat | 294 – 327 | 34 | TPR 4 | ||||||
| Domain | 462 – 670 | 209 | PKHD | ||||||
| Coiled coil | 394 – 441 | 48 | Potential | ||||||
| Motif | 722 – 725 | 4 | Prevents secretion from ER Potential | ||||||
Sites | |||||||||
| Active site | 662 | 1 | By similarity | ||||||
| Metal binding | 580 | 1 | Iron | ||||||
| Metal binding | 582 | 1 | Iron | ||||||
| Metal binding | 652 | 1 | Iron | ||||||
Amino acid modifications | |||||||||
| Glycosylation | 82 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 460 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 533 | 1 | N-linked (GlcNAc...) Potential | ||||||
Sequences
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References
| [1] | "Prolyl 3-hydroxylase 1, enzyme characterization and identification of a novel family of enzymes." Vranka J.A., Sakai L.Y., Bachinger H.P. J. Biol. Chem. 279:23615-23621(2004) [PubMed: 15044469] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, COMPONENT OF A UNFOLDED COLLAGEN-BINDING COMPLEX INCLUDING P3H1; CYPB AND CRTAP, CHARACTERIZATION, TISSUE SPECIFICITY. Tissue: Embryonic fibroblast. |
Cross-references
Sequence databases | |
|---|---|
| AY463528 mRNA. Translation: AAS45237.1. | |
| RefSeq | NP_001001529.1. |
| UniGene | Gga.9766 |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | Q6JHU8. 1 interaction. |
Genome annotation databases | |
| GeneID | 414142. |
| KEGG | gga:414142. |
Phylogenomic databases | |
| HOVERGEN | Q6JHU8. |
Family and domain databases | |
| InterPro | IPR005123. 2OG-FeII_Oase. IPR000886. ER_targeting_sequence. IPR006620. Pro_4_hyd_alph. IPR013026. TPR_region. [Graphical view] |
| Pfam | PF03171. 2OG-FeII_Oxy. 1 hit. [Graphical view] |
| SMART | SM00702. P4Hc. 1 hit. [Graphical view] |
| PROSITE | PS00014. ER_TARGET. 1 hit. PS50005. TPR. False negative. PS50293. TPR_REGION. False negative. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | P3H1_CHICK | ||||||||
| Accession | Primary (citable) accession number: Q6JHU8 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||

Clusters with


