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Q6JHU8 (P3H1_CHICK) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Prolyl 3-hydroxylase 1

EC=1.14.11.7
Alternative name(s):
Leucine- and proline-enriched proteoglycan 1 homolog
Short name=Leprecan-1 homolog
Gene names
Name:LEPRE1
Synonyms:P3H1
OrganismGallus gallus (Chicken) [Reference proteome]
Taxonomic identifier9031 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus

Protein attributes

Sequence length725 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Has prolyl 3-hydroxylase activity catalyzing the post-translational formation of 3-hydroxyproline in -Xaa-Pro-Gly-sequences in collagens, especially types IV and V. May be involved in the secretoty pathway of cells. Has growth suppressive activity in fibroblasts By similarity.

Catalytic activity

L-proline-[procollagen] + 2-oxoglutarate + O2 = trans-3-hydroxy-L-proline-[procollagen] + succinate + CO2.

Cofactor

Iron By similarity.

Ascorbate By similarity.

Subunit structure

Binds unfolded collagen in a complex with CYPB and CRTAP.

Subcellular location

Endoplasmic reticulum By similarity.

Tissue specificity

Expressed in embryonic dermis, tendon, cartilage, liver and kidney. Expression in the kidney is restricted to the calyx. In the liver, expression is restricted to the interlobular septum. Ref.1

Sequence similarities

Belongs to the leprecan family.

Contains 1 Fe2OG dioxygenase domain.

Contains 4 TPR repeats.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

CRTAPQ908303EBI-1169258,EBI-1169253

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1414 Potential
Chain15 – 725711Prolyl 3-hydroxylase 1
PRO_0000240355

Regions

Repeat36 – 6934TPR 1
Repeat136 – 16934TPR 2
Repeat198 – 23134TPR 3
Repeat294 – 32734TPR 4
Domain557 – 671115Fe2OG dioxygenase
Coiled coil394 – 44148 Potential
Motif722 – 7254Prevents secretion from ER Potential

Sites

Active site6621 By similarity
Metal binding5801Iron
Metal binding5821Iron
Metal binding6521Iron

Amino acid modifications

Glycosylation821N-linked (GlcNAc...) Potential
Glycosylation4601N-linked (GlcNAc...) Potential
Glycosylation5331N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q6JHU8 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 2D227635DA36B605

FASTA72581,676
        10         20         30         40         50         60 
MALLLPLLPL LVWAAGPPGP LGPPEHSEPP LPAEPPDALF AAGAEAYARG DWPAVVLQME 

        70         80         90        100        110        120 
RALRARAAIR ARSVRCRLRC ANATAVVPTD GLEPTLHDLL FFRGLLRRAA CLRGCGPTQP 

       130        140        150        160        170        180 
SRYRLGEELE REFRKRSPYN YLQVAYFKIN KVAKAVAAAH TFFVANPEHV EMKQNLEYYQ 

       190        200        210        220        230        240 
MMAGVRESDF ADLEARPHMT EFRLGVRFYS EEQPAAAVLH LEKALQEYFV ADTECRALCE 

       250        260        270        280        290        300 
GPYDYEGYNY LEYNADLFQA MTDHYMQVLS CKQGCVTELA SQPGREKPLE DFLPSHFNYL 

       310        320        330        340        350        360 
QFAYYNNGNY EKAIECAKTY LLFFPNDEVM NQNLAYYTAV LGEDLARPIE PRKEIQAYRQ 

       370        380        390        400        410        420 
RSLMEKELLF FSYDVFGIPF VDPDTWTPEE VIPKRLREKQ KVERETAARI SEEIGNLMKE 

       430        440        450        460        470        480 
IETLVEEKAK ESAEMSKFIR EGGPLVYEGA SVTMNSKSLN GSQRVVVDGV LSAEECRELQ 

       490        500        510        520        530        540 
RLTNAAASAG DGYRGKTSPH TPSETFYGVT VLKALKLGQE GKVPLQSAHL YYNVTEKVRH 

       550        560        570        580        590        600 
MMESYFRLEV PLHFSYSHLV CRTAIDEKQE GRSDNSHEVH VDNCILNAEA LVCVKEPPAY 

       610        620        630        640        650        660 
TFRDYSAILY LNGDFEGGAF YFTELDAKTQ TAEVQPQCGR AVGFSSGSEN PHGVKAVTKG 

       670        680        690        700        710        720 
QRCAIALWFT LDPRHSERER VQADDLVKML FRTEEVDLLQ ETSAEQEPTA ATSTAGLHAA 


GKDEL 

« Hide

References

[1]"Prolyl 3-hydroxylase 1, enzyme characterization and identification of a novel family of enzymes."
Vranka J.A., Sakai L.Y., Bachinger H.P.
J. Biol. Chem. 279:23615-23621(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, COMPONENT OF A UNFOLDED COLLAGEN-BINDING COMPLEX INCLUDING P3H1; CYPB AND CRTAP, CHARACTERIZATION, TISSUE SPECIFICITY.
Tissue: Embryonic fibroblast.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY463528 mRNA. Translation: AAS45237.1.
RefSeqNP_001001529.1. NM_001001529.1.
UniGeneGga.9766.

3D structure databases

ProteinModelPortalQ6JHU8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ6JHU8. 1 interaction.
STRING9031.ENSGALP00000007707.

Proteomic databases

PaxDbQ6JHU8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSGALT00000007719; ENSGALP00000007707; ENSGALG00000004833.
GeneID414142.
KEGGgga:414142.

Organism-specific databases

CTD64175.

Phylogenomic databases

eggNOGNOG269251.
GeneTreeENSGT00550000074573.
HOVERGENHBG053224.
KOK08134.
PhylomeDBQ6JHU8.

Family and domain databases

Gene3D1.25.40.10. 3 hits.
InterProIPR005123. Oxoglu/Fe-dep_dioxygenase.
IPR006620. Pro_4_hyd_alph.
IPR011990. TPR-like_helical.
[Graphical view]
PfamPF13640. 2OG-FeII_Oxy_3. 1 hit.
[Graphical view]
SMARTSM00702. P4Hc. 1 hit.
[Graphical view]
PROSITEPS00014. ER_TARGET. 1 hit.
PS51471. FE2OG_OXY. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20818679.
PROQ6JHU8.

Entry information

Entry nameP3H1_CHICK
AccessionPrimary (citable) accession number: Q6JHU8
Entry history
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: July 5, 2004
Last modified: April 16, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families