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Protein

Prolyl 3-hydroxylase 2

Gene

LEPREL1

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Has prolyl 3-hydroxylase activity catalyzing the post-translational formation of 3-hydroxyproline in -Xaa-Pro-Gly-sequences in collagens, especially types II, IV and V.By similarity

Catalytic activityi

L-proline-[procollagen] + 2-oxoglutarate + O2 = trans-3-hydroxy-L-proline-[procollagen] + succinate + CO2.

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi566 – 5661Iron
Metal bindingi568 – 5681Iron
Metal bindingi638 – 6381Iron
Active sitei648 – 6481By similarity

GO - Molecular functioni

  • iron ion binding Source: InterPro
  • L-ascorbic acid binding Source: UniProtKB-KW
  • procollagen-proline 3-dioxygenase activity Source: UniProtKB

GO - Biological processi

  • collagen metabolic process Source: UniProtKB
  • negative regulation of cell proliferation Source: UniProtKB
  • peptidyl-proline hydroxylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Ligandi

Iron, Metal-binding, Vitamin C

Names & Taxonomyi

Protein namesi
Recommended name:
Prolyl 3-hydroxylase 2 (EC:1.14.11.7)
Alternative name(s):
Leprecan-like protein 1
Gene namesi
Name:LEPREL1
Synonyms:P3H2
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
ProteomesiUP000000539 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence AnalysisAdd
BLAST
Chaini24 – 694671Prolyl 3-hydroxylase 2PRO_0000240359Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi446 – 4461N-linked (GlcNAc...)Sequence Analysis
Glycosylationi535 – 5351N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ6JHU7.

Interactioni

Protein-protein interaction databases

STRINGi9031.ENSGALP00000011808.

Structurei

3D structure databases

ProteinModelPortaliQ6JHU7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati35 – 6834TPR 1Add
BLAST
Repeati136 – 16934TPR 2Add
BLAST
Repeati196 – 22934TPR 3Add
BLAST
Repeati292 – 32534TPR 4Add
BLAST
Domaini543 – 657115Fe2OG dioxygenasePROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili386 – 41833Sequence AnalysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi691 – 6944Prevents secretion from ERPROSITE-ProRule annotation

Sequence similaritiesi

Belongs to the leprecan family.Curated
Contains 1 Fe2OG dioxygenase domain.PROSITE-ProRule annotation
Contains 4 TPR repeats.Curated

Keywords - Domaini

Coiled coil, Repeat, Signal, TPR repeat

Phylogenomic databases

eggNOGiNOG269251.
HOGENOMiHOG000231087.
HOVERGENiHBG053224.
InParanoidiQ6JHU7.
PhylomeDBiQ6JHU7.

Family and domain databases

Gene3Di1.25.40.10. 3 hits.
InterProiIPR005123. Oxoglu/Fe-dep_dioxygenase.
IPR006620. Pro_4_hyd_alph.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PfamiPF13640. 2OG-FeII_Oxy_3. 1 hit.
[Graphical view]
SMARTiSM00702. P4Hc. 1 hit.
[Graphical view]
PROSITEiPS00014. ER_TARGET. 1 hit.
PS51471. FE2OG_OXY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6JHU7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPGSRSWGA VLLLAAMLPA ACGSCGADGG PLEPFDALYA SGVEAYYGGD
60 70 80 90 100
FAGAARCLEQ ALRSRRELRA ERLRCRRRCR GQVRLAALGA GPAGELPFFG
110 120 130 140 150
ALLRRAGCLR SCEEPRLGAA SRHRAAEEVR SDFQRRVPYS YLQRAYIQLN
160 170 180 190 200
KLEEAANAAH TFFMANPEHM EIQQDIENYK TTAGKVSLID LEAKPHMEDY
210 220 230 240 250
SAGVRHYDKE EYGLAITFLE RALEGYYAED EDCQIMCEGP QRFEEHEYLE
260 270 280 290 300
YKAGLYEAIA DHYMQVLACK HDCIRELATR SGRISPIENF LPLHYDYLQF
310 320 330 340 350
AYYRVGDYVK ALECARSYLL FHPDDEDVLE NAAYYEGLLE GTVDPATIKP
360 370 380 390 400
RKEAKALLRR HKLESHLLRV AAVGLGFTYT EPNYWKRYGA RQDEHSVPSS
410 420 430 440 450
ISSEPEDGPR LSLTKKPTPK PDRELKEGGP LLYSDVKFVY NSQQLNGTQR
460 470 480 490 500
VLLDNVISEE QCRELHRVAS GIMLAGDGYR GKTSPHTPNE RFEGATVLKA
510 520 530 540 550
LKYGYEGRVP LKSARLFYDI SEKARRIVES YFMLNSTLYF SYTHLVCRTA
560 570 580 590 600
LSGQQERRND LSHPIHADNC LLDPEANECW KEPPAYTFRD YSALLYMNAD
610 620 630 640 650
FEGGEFIFTE MDAKTVTASI KPKCGRMVSF SSGGENPHGV KAVTKGQRCA
660 670 680 690
VALWFTLDPL YRELERIQAD EVIAMLDQEH VGRSEMNINP KDEL
Length:694
Mass (Da):78,430
Last modified:July 5, 2004 - v1
Checksum:i6263153C4577AFBD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY463529 mRNA. Translation: AAS45238.1.
RefSeqiNP_001001530.1. NM_001001530.1.
UniGeneiGga.16244.

Genome annotation databases

GeneIDi414143.
KEGGigga:414143.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY463529 mRNA. Translation: AAS45238.1.
RefSeqiNP_001001530.1. NM_001001530.1.
UniGeneiGga.16244.

3D structure databases

ProteinModelPortaliQ6JHU7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9031.ENSGALP00000011808.

Proteomic databases

PaxDbiQ6JHU7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi414143.
KEGGigga:414143.

Organism-specific databases

CTDi566697.

Phylogenomic databases

eggNOGiNOG269251.
HOGENOMiHOG000231087.
HOVERGENiHBG053224.
InParanoidiQ6JHU7.
PhylomeDBiQ6JHU7.

Miscellaneous databases

NextBioi20818680.
PROiQ6JHU7.

Family and domain databases

Gene3Di1.25.40.10. 3 hits.
InterProiIPR005123. Oxoglu/Fe-dep_dioxygenase.
IPR006620. Pro_4_hyd_alph.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PfamiPF13640. 2OG-FeII_Oxy_3. 1 hit.
[Graphical view]
SMARTiSM00702. P4Hc. 1 hit.
[Graphical view]
PROSITEiPS00014. ER_TARGET. 1 hit.
PS51471. FE2OG_OXY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Prolyl 3-hydroxylase 1, enzyme characterization and identification of a novel family of enzymes."
    Vranka J.A., Sakai L.Y., Bachinger H.P.
    J. Biol. Chem. 279:23615-23621(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiP3H2_CHICK
AccessioniPrimary (citable) accession number: Q6JHU7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: July 5, 2004
Last modified: April 29, 2015
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.