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Protein

Prolyl 3-hydroxylase 2

Gene

P3H2

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Prolyl 3-hydroxylase that catalyzes the post-translational formation of 3-hydroxyproline on collagens (By similarity). Contributes to proline 3-hydroxylation of collagen COL4A1 and COL1A1 in tendons, the eye sclera and in the eye lens capsule (By similarity). Has high activity with the type IV collagen COL4A1, and lower activity with COL1A1. Catalyzes hydroxylation of the first Pro in Gly-Pro-Hyp sequences where Hyp is 4-hydroxyproline. Has no activity on substrates that lack 4-hydroxyproline in the third position (By similarity).By similarity

Catalytic activityi

L-proline-[procollagen] + 2-oxoglutarate + O2 = trans-3-hydroxy-L-proline-[procollagen] + succinate + CO2.By similarity

Cofactori

Protein has several cofactor binding sites:
  • Fe cationBy similarity
  • L-ascorbateBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi566Iron1
Metal bindingi568Iron1
Metal bindingi638Iron1
Active sitei648By similarity1

GO - Molecular functioni

  • iron ion binding Source: InterPro
  • L-ascorbic acid binding Source: UniProtKB-KW
  • procollagen-proline 3-dioxygenase activity Source: UniProtKB

GO - Biological processi

  • collagen metabolic process Source: UniProtKB
  • negative regulation of cell proliferation Source: UniProtKB
  • peptidyl-proline hydroxylation Source: UniProtKB

Keywordsi

Molecular functionDioxygenase, Oxidoreductase
LigandIron, Metal-binding, Vitamin C

Enzyme and pathway databases

ReactomeiR-GGA-1650814 Collagen biosynthesis and modifying enzymes

Names & Taxonomyi

Protein namesi
Recommended name:
Prolyl 3-hydroxylase 2By similarity (EC:1.14.11.7By similarity)
Alternative name(s):
Leprecan-like protein 1By similarity
Gene namesi
Name:P3H2By similarity
Synonyms:LEPREL1By similarity
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Chromosome 9

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Sarcoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000024035924 – 694Prolyl 3-hydroxylase 2Add BLAST671

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi446N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi535N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ6JHU7

Structurei

3D structure databases

ProteinModelPortaliQ6JHU7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati35 – 68TPR 1Add BLAST34
Repeati136 – 169TPR 2Add BLAST34
Repeati196 – 229TPR 3Add BLAST34
Repeati292 – 325TPR 4Add BLAST34
Domaini543 – 657Fe2OG dioxygenasePROSITE-ProRule annotationAdd BLAST115

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili386 – 418Sequence analysisAdd BLAST33

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi691 – 694Prevents secretion from ERPROSITE-ProRule annotation4

Sequence similaritiesi

Belongs to the leprecan family.Curated

Keywords - Domaini

Coiled coil, Repeat, Signal, TPR repeat

Phylogenomic databases

eggNOGiKOG4459 Eukaryota
ENOG410XPXF LUCA
GeneTreeiENSGT00550000074573
HOGENOMiHOG000231087
HOVERGENiHBG053224
InParanoidiQ6JHU7
KOiK22459
PhylomeDBiQ6JHU7

Family and domain databases

InterProiView protein in InterPro
IPR005123 Oxoglu/Fe-dep_dioxygenase
IPR006620 Pro_4_hyd_alph
IPR011990 TPR-like_helical_dom_sf
PfamiView protein in Pfam
PF13640 2OG-FeII_Oxy_3, 1 hit
SMARTiView protein in SMART
SM00702 P4Hc, 1 hit
SUPFAMiSSF48452 SSF48452, 4 hits
PROSITEiView protein in PROSITE
PS00014 ER_TARGET, 1 hit
PS51471 FE2OG_OXY, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6JHU7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPGSRSWGA VLLLAAMLPA ACGSCGADGG PLEPFDALYA SGVEAYYGGD
60 70 80 90 100
FAGAARCLEQ ALRSRRELRA ERLRCRRRCR GQVRLAALGA GPAGELPFFG
110 120 130 140 150
ALLRRAGCLR SCEEPRLGAA SRHRAAEEVR SDFQRRVPYS YLQRAYIQLN
160 170 180 190 200
KLEEAANAAH TFFMANPEHM EIQQDIENYK TTAGKVSLID LEAKPHMEDY
210 220 230 240 250
SAGVRHYDKE EYGLAITFLE RALEGYYAED EDCQIMCEGP QRFEEHEYLE
260 270 280 290 300
YKAGLYEAIA DHYMQVLACK HDCIRELATR SGRISPIENF LPLHYDYLQF
310 320 330 340 350
AYYRVGDYVK ALECARSYLL FHPDDEDVLE NAAYYEGLLE GTVDPATIKP
360 370 380 390 400
RKEAKALLRR HKLESHLLRV AAVGLGFTYT EPNYWKRYGA RQDEHSVPSS
410 420 430 440 450
ISSEPEDGPR LSLTKKPTPK PDRELKEGGP LLYSDVKFVY NSQQLNGTQR
460 470 480 490 500
VLLDNVISEE QCRELHRVAS GIMLAGDGYR GKTSPHTPNE RFEGATVLKA
510 520 530 540 550
LKYGYEGRVP LKSARLFYDI SEKARRIVES YFMLNSTLYF SYTHLVCRTA
560 570 580 590 600
LSGQQERRND LSHPIHADNC LLDPEANECW KEPPAYTFRD YSALLYMNAD
610 620 630 640 650
FEGGEFIFTE MDAKTVTASI KPKCGRMVSF SSGGENPHGV KAVTKGQRCA
660 670 680 690
VALWFTLDPL YRELERIQAD EVIAMLDQEH VGRSEMNINP KDEL
Length:694
Mass (Da):78,430
Last modified:July 5, 2004 - v1
Checksum:i6263153C4577AFBD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY463529 mRNA Translation: AAS45238.1
RefSeqiNP_001001530.1, NM_001001530.1
UniGeneiGga.16244

Genome annotation databases

EnsembliENSGALT00000046911; ENSGALP00000046986; ENSGALG00000040866
GeneIDi414143
KEGGigga:414143

Similar proteinsi

Entry informationi

Entry nameiP3H2_CHICK
AccessioniPrimary (citable) accession number: Q6JHU7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: July 5, 2004
Last modified: April 25, 2018
This is version 96 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health