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Q6JHU7

- P3H2_CHICK

UniProt

Q6JHU7 - P3H2_CHICK

Protein

Prolyl 3-hydroxylase 2

Gene

LEPREL1

Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Has prolyl 3-hydroxylase activity catalyzing the post-translational formation of 3-hydroxyproline in -Xaa-Pro-Gly-sequences in collagens, especially types II, IV and V.By similarity

    Catalytic activityi

    L-proline-[procollagen] + 2-oxoglutarate + O2 = trans-3-hydroxy-L-proline-[procollagen] + succinate + CO2.

    Cofactori

    Iron.By similarity
    Ascorbate.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi566 – 5661Iron
    Metal bindingi568 – 5681Iron
    Metal bindingi638 – 6381Iron
    Active sitei648 – 6481By similarity

    GO - Molecular functioni

    1. iron ion binding Source: InterPro
    2. L-ascorbic acid binding Source: UniProtKB-KW
    3. procollagen-proline 3-dioxygenase activity Source: UniProtKB

    GO - Biological processi

    1. collagen metabolic process Source: UniProtKB
    2. negative regulation of cell proliferation Source: UniProtKB
    3. peptidyl-proline hydroxylation Source: UniProtKB

    Keywords - Molecular functioni

    Dioxygenase, Oxidoreductase

    Keywords - Ligandi

    Iron, Metal-binding, Vitamin C

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Prolyl 3-hydroxylase 2 (EC:1.14.11.7)
    Alternative name(s):
    Leprecan-like protein 1
    Gene namesi
    Name:LEPREL1
    Synonyms:P3H2
    OrganismiGallus gallus (Chicken)
    Taxonomic identifieri9031 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
    ProteomesiUP000000539: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. basement membrane Source: UniProtKB
    2. endoplasmic reticulum Source: UniProtKB
    3. Golgi apparatus Source: UniProtKB

    Keywords - Cellular componenti

    Endoplasmic reticulum, Golgi apparatus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2323Sequence AnalysisAdd
    BLAST
    Chaini24 – 694671Prolyl 3-hydroxylase 2PRO_0000240359Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi446 – 4461N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi535 – 5351N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ6JHU7.

    Interactioni

    Protein-protein interaction databases

    STRINGi9031.ENSGALP00000011808.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6JHU7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati35 – 6834TPR 1Add
    BLAST
    Repeati136 – 16934TPR 2Add
    BLAST
    Repeati196 – 22934TPR 3Add
    BLAST
    Repeati292 – 32534TPR 4Add
    BLAST
    Domaini543 – 657115Fe2OG dioxygenasePROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili386 – 41833Sequence AnalysisAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi691 – 6944Prevents secretion from ERPROSITE-ProRule annotation

    Sequence similaritiesi

    Belongs to the leprecan family.Curated
    Contains 1 Fe2OG dioxygenase domain.PROSITE-ProRule annotation
    Contains 4 TPR repeats.Curated

    Keywords - Domaini

    Coiled coil, Repeat, Signal, TPR repeat

    Phylogenomic databases

    eggNOGiNOG269251.
    HOGENOMiHOG000231087.
    HOVERGENiHBG053224.
    InParanoidiQ6JHU7.
    PhylomeDBiQ6JHU7.

    Family and domain databases

    Gene3Di1.25.40.10. 3 hits.
    InterProiIPR005123. Oxoglu/Fe-dep_dioxygenase.
    IPR006620. Pro_4_hyd_alph.
    IPR011990. TPR-like_helical.
    [Graphical view]
    PfamiPF13640. 2OG-FeII_Oxy_3. 1 hit.
    [Graphical view]
    SMARTiSM00702. P4Hc. 1 hit.
    [Graphical view]
    PROSITEiPS00014. ER_TARGET. 1 hit.
    PS51471. FE2OG_OXY. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q6JHU7-1 [UniParc]FASTAAdd to Basket

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    MAPGSRSWGA VLLLAAMLPA ACGSCGADGG PLEPFDALYA SGVEAYYGGD    50
    FAGAARCLEQ ALRSRRELRA ERLRCRRRCR GQVRLAALGA GPAGELPFFG 100
    ALLRRAGCLR SCEEPRLGAA SRHRAAEEVR SDFQRRVPYS YLQRAYIQLN 150
    KLEEAANAAH TFFMANPEHM EIQQDIENYK TTAGKVSLID LEAKPHMEDY 200
    SAGVRHYDKE EYGLAITFLE RALEGYYAED EDCQIMCEGP QRFEEHEYLE 250
    YKAGLYEAIA DHYMQVLACK HDCIRELATR SGRISPIENF LPLHYDYLQF 300
    AYYRVGDYVK ALECARSYLL FHPDDEDVLE NAAYYEGLLE GTVDPATIKP 350
    RKEAKALLRR HKLESHLLRV AAVGLGFTYT EPNYWKRYGA RQDEHSVPSS 400
    ISSEPEDGPR LSLTKKPTPK PDRELKEGGP LLYSDVKFVY NSQQLNGTQR 450
    VLLDNVISEE QCRELHRVAS GIMLAGDGYR GKTSPHTPNE RFEGATVLKA 500
    LKYGYEGRVP LKSARLFYDI SEKARRIVES YFMLNSTLYF SYTHLVCRTA 550
    LSGQQERRND LSHPIHADNC LLDPEANECW KEPPAYTFRD YSALLYMNAD 600
    FEGGEFIFTE MDAKTVTASI KPKCGRMVSF SSGGENPHGV KAVTKGQRCA 650
    VALWFTLDPL YRELERIQAD EVIAMLDQEH VGRSEMNINP KDEL 694
    Length:694
    Mass (Da):78,430
    Last modified:July 5, 2004 - v1
    Checksum:i6263153C4577AFBD
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY463529 mRNA. Translation: AAS45238.1.
    RefSeqiNP_001001530.1. NM_001001530.1.
    UniGeneiGga.16244.

    Genome annotation databases

    GeneIDi414143.
    KEGGigga:414143.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY463529 mRNA. Translation: AAS45238.1 .
    RefSeqi NP_001001530.1. NM_001001530.1.
    UniGenei Gga.16244.

    3D structure databases

    ProteinModelPortali Q6JHU7.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9031.ENSGALP00000011808.

    Proteomic databases

    PaxDbi Q6JHU7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 414143.
    KEGGi gga:414143.

    Organism-specific databases

    CTDi 55214.

    Phylogenomic databases

    eggNOGi NOG269251.
    HOGENOMi HOG000231087.
    HOVERGENi HBG053224.
    InParanoidi Q6JHU7.
    PhylomeDBi Q6JHU7.

    Miscellaneous databases

    NextBioi 20818680.
    PROi Q6JHU7.

    Family and domain databases

    Gene3Di 1.25.40.10. 3 hits.
    InterProi IPR005123. Oxoglu/Fe-dep_dioxygenase.
    IPR006620. Pro_4_hyd_alph.
    IPR011990. TPR-like_helical.
    [Graphical view ]
    Pfami PF13640. 2OG-FeII_Oxy_3. 1 hit.
    [Graphical view ]
    SMARTi SM00702. P4Hc. 1 hit.
    [Graphical view ]
    PROSITEi PS00014. ER_TARGET. 1 hit.
    PS51471. FE2OG_OXY. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Prolyl 3-hydroxylase 1, enzyme characterization and identification of a novel family of enzymes."
      Vranka J.A., Sakai L.Y., Bachinger H.P.
      J. Biol. Chem. 279:23615-23621(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].

    Entry informationi

    Entry nameiP3H2_CHICK
    AccessioniPrimary (citable) accession number: Q6JHU7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 27, 2006
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 75 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3