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Q6JE36 (NDRG1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein NDRG1
Alternative name(s):
N-myc downstream-regulated gene 1 protein
Short name=Protein Ndr1
Gene names
Name:Ndrg1
Synonyms:Ndr1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length394 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Stress-responsive protein involved in hormone responses, cell growth, and differentiation. Acts as a tumor suppressor in many cell types. Necessary but not sufficient for p53/TP53-mediated caspase activation and apoptosis. Has a role in cell trafficking notably of the Schwann cell and is necessary for the maintenance and development of the peripheral nerve myelin sheath. Required for vesicular recycling of CDH1 and TF. May also function in lipid trafficking. Protects cells from spindle disruption damage. Functions in p53/TP53-dependent mitotic spindle checkpoint. Regulates microtubule dynamics and maintains euploidy By similarity.

Subunit structure

Interacts with RAB4A (membrane-bound form); the interaction involves NDRG1 in vesicular recycling of CDH1. Interacts with APOA1, APOA2, PRA1 and RTN1 By similarity.

Subcellular location

Cytoplasmcytosol By similarity. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity. Nucleus By similarity. Cell membrane By similarity. Note: Mainly cytoplasmic but differentially localized to other regions. Associates with the plasma membrane in intestinal epithelia and lactating mammary gland. Translocated to the nucleus in a p53/TP53-dependent manner. In prostate epithelium and placental chorion, located in both the cytoplasm and in the nucleus. No nuclear localization in colon epithelium cells. In intestinal mucosa, prostate and renal cortex, located predominantly adjacent to adherens junctions. Cytoplasmic with granular staining in proximal tubular cells of the kidney and salivary gland ducts. Recruits to the membrane of recycling/sorting and late endosomes via binding to phosphatidylinositol 4-phosphate. Associates with microtubules. Colocalizes with TUBG1 in the centrosome. Cytoplasmic location increased with hypoxia. Phosphorylated form found associated with centromeres during S-phase of mitosis and with the plasma membrane By similarity.

Post-translational modification

Under stress conditions, phosphorylated in the C-terminal on many serine and threonine residues. Phosphorylated in vitro by PKA. Phosphorylation enhanced by increased intracellular cAMP levels. Homocysteine induces dephosphorylation. Phosphorylation by SGK1 is cell cycle dependent By similarity.

Sequence similarities

Belongs to the NDRG family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 394393Protein NDRG1
PRO_0000270758

Regions

Repeat339 – 348101
Repeat349 – 358102
Repeat359 – 368103
Region339 – 368303 X 10 AA tandem repeats of G-[PST]-R-S-R-S-H-T-S-E

Amino acid modifications

Modified residue21N-acetylserine By similarity
Modified residue3261Phosphoserine By similarity
Modified residue3281Phosphothreonine; by SGK1 By similarity
Modified residue3301Phosphoserine; by SGK1 By similarity
Modified residue3321Phosphoserine; by SGK1 By similarity
Modified residue3331Phosphoserine By similarity
Modified residue3361Phosphoserine By similarity
Modified residue3461Phosphothreonine; by SGK1 By similarity
Modified residue3561Phosphothreonine; by SGK1 By similarity
Modified residue3641Phosphoserine By similarity
Modified residue3661Phosphothreonine; by SGK1 By similarity
Modified residue3751Phosphothreonine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6JE36 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 42B2B95FBDBC9A16

FASTA39442,955
        10         20         30         40         50         60 
MSRELHDVDL AEVKPLVEKG ESITGLLQEF DVQEQDIETL HGSLHVTLCG TPKGNRPVIL 

        70         80         90        100        110        120 
TYHDIGMNHK TCYNPLFNSE DMQEITQHFA VCHVDAPGQQ DGAPSFPVGY MYPSMDQLAE 

       130        140        150        160        170        180 
MLPGVLHKFG LKSVIGMGTG AGAYILTRFA LNNPEMVEGL VLMNVNPCAE GWMDWAASKI 

       190        200        210        220        230        240 
SGWTQALPDM VVSHLFGKEE IHSNVEVVHT YRQHILNDMN PSNLHLFISA YNSRRDLEIE 

       250        260        270        280        290        300 
RPMPGTHTVT LQCPALLVVG DNSPAVDAVV ECNSKLDPTK TTLLKMADCG GLPQISQPAK 

       310        320        330        340        350        360 
LAEAFKYFVQ GMGYMPSASM TRLMRSRTAS GSSVTSLEGT RSRSHTSEGP RSRSHTSEGS 

       370        380        390 
RSRSHTSEDA RLNITPSSGA TGNNAGPKSM EVSC 

« Hide

References

« Hide 'large scale' references
[1]Wang X., Jing N.
Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Kidney.
[3]Lubec G., Afjehi-Sadat L., Chen W.-Q.
Submitted (APR-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 133-148; 213-234 AND 328-341, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: Sprague-Dawley.
Tissue: Hippocampus and Spinal cord.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY500369 mRNA. Translation: AAS78638.1.
BC081898 mRNA. Translation: AAH81898.1.
RefSeqNP_001011991.1. NM_001011991.1.
UniGeneRn.153992.

3D structure databases

ProteinModelPortalQ6JE36.
SMRQ6JE36. Positions 31-311.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000010811.

PTM databases

PhosphoSiteQ6JE36.

Proteomic databases

PaxDbQ6JE36.
PRIDEQ6JE36.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000010811; ENSRNOP00000010811; ENSRNOG00000007393.
GeneID299923.
KEGGrno:299923.
UCSCRGD:1307303. rat.

Organism-specific databases

CTD10397.
RGD1307303. Ndrg1.

Phylogenomic databases

eggNOGNOG310435.
GeneTreeENSGT00390000001874.
HOGENOMHOG000230891.
HOVERGENHBG052591.
InParanoidQ6JE36.
OMATLHGSIH.
OrthoDBEOG7KH9JS.
PhylomeDBQ6JE36.
TreeFamTF313168.

Gene expression databases

GenevestigatorQ6JE36.

Family and domain databases

InterProIPR004142. Ndr.
[Graphical view]
PANTHERPTHR11034. PTHR11034. 1 hit.
PfamPF03096. Ndr. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio645992.

Entry information

Entry nameNDRG1_RAT
AccessionPrimary (citable) accession number: Q6JE36
Entry history
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: July 5, 2004
Last modified: April 16, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families