Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protein NDRG1

Gene

Ndrg1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Stress-responsive protein involved in hormone responses, cell growth, and differentiation. Acts as a tumor suppressor in many cell types. Necessary but not sufficient for p53/TP53-mediated caspase activation and apoptosis. Has a role in cell trafficking notably of the Schwann cell and is necessary for the maintenance and development of the peripheral nerve myelin sheath. Required for vesicular recycling of CDH1 and TF. May also function in lipid trafficking. Protects cells from spindle disruption damage. Functions in p53/TP53-dependent mitotic spindle checkpoint. Regulates microtubule dynamics and maintains euploidy (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Protein family/group databases

ESTHERiratno-q6je36. Ndr_family.
MEROPSiS33.988.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein NDRG1
Alternative name(s):
N-myc downstream-regulated gene 1 protein
Short name:
Protein Ndr1
Gene namesi
Name:Ndrg1
Synonyms:Ndr1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi1307303. Ndrg1.

Subcellular locationi

  • Cytoplasmcytosol By similarity
  • Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity
  • Nucleus By similarity
  • Cell membrane By similarity

  • Note: Mainly cytoplasmic but differentially localized to other regions. Associates with the plasma membrane in intestinal epithelia and lactating mammary gland. Translocated to the nucleus in a p53/TP53-dependent manner. In prostate epithelium and placental chorion, located in both the cytoplasm and in the nucleus. No nuclear localization in colon epithelium cells. In intestinal mucosa, prostate and renal cortex, located predominantly adjacent to adherens junctions. Cytoplasmic with granular staining in proximal tubular cells of the kidney and salivary gland ducts. Recruits to the membrane of recycling/sorting and late endosomes via binding to phosphatidylinositol 4-phosphate. Associates with microtubules. Colocalizes with TUBG1 in the centrosome. Cytoplasmic location increased with hypoxia. Phosphorylated form found associated with centromeres during S-phase of mitosis and with the plasma membrane (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoskeleton, Membrane, Microtubule, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 394393Protein NDRG1PRO_0000270758Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei326 – 3261PhosphoserineBy similarity
Modified residuei328 – 3281Phosphothreonine; by SGK1By similarity
Modified residuei330 – 3301Phosphoserine; by SGK1By similarity
Modified residuei332 – 3321Phosphoserine; by SGK1By similarity
Modified residuei333 – 3331PhosphoserineBy similarity
Modified residuei336 – 3361PhosphoserineBy similarity
Modified residuei346 – 3461Phosphothreonine; by SGK1By similarity
Modified residuei356 – 3561Phosphothreonine; by SGK1By similarity
Modified residuei364 – 3641PhosphoserineBy similarity
Modified residuei366 – 3661Phosphothreonine; by SGK1By similarity
Modified residuei375 – 3751PhosphothreonineBy similarity

Post-translational modificationi

Under stress conditions, phosphorylated in the C-terminal on many serine and threonine residues. Phosphorylated in vitro by PKA. Phosphorylation enhanced by increased intracellular cAMP levels. Homocysteine induces dephosphorylation. Phosphorylation by SGK1 is cell cycle dependent (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ6JE36.
PRIDEiQ6JE36.

PTM databases

PhosphoSiteiQ6JE36.

Expressioni

Gene expression databases

GenevisibleiQ6JE36. RN.

Interactioni

Subunit structurei

Interacts with RAB4A (membrane-bound form); the interaction involves NDRG1 in vesicular recycling of CDH1. Interacts with APOA1, APOA2, PRA1 and RTN1 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000010811.

Structurei

3D structure databases

ProteinModelPortaliQ6JE36.
SMRiQ6JE36. Positions 31-311.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati339 – 348101
Repeati349 – 358102
Repeati359 – 368103

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni339 – 368303 X 10 AA tandem repeats of G-[PST]-R-S-R-S-H-T-S-EAdd
BLAST

Sequence similaritiesi

Belongs to the NDRG family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG310435.
GeneTreeiENSGT00390000001874.
HOGENOMiHOG000230891.
HOVERGENiHBG052591.
InParanoidiQ6JE36.
KOiK18266.
OMAiKTCFNPL.
OrthoDBiEOG7KH9JS.
PhylomeDBiQ6JE36.
TreeFamiTF313168.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR004142. NDRG.
IPR030693. NDRG1.
[Graphical view]
PANTHERiPTHR11034. PTHR11034. 1 hit.
PTHR11034:SF18. PTHR11034:SF18. 1 hit.
PfamiPF03096. Ndr. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6JE36-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRELHDVDL AEVKPLVEKG ESITGLLQEF DVQEQDIETL HGSLHVTLCG
60 70 80 90 100
TPKGNRPVIL TYHDIGMNHK TCYNPLFNSE DMQEITQHFA VCHVDAPGQQ
110 120 130 140 150
DGAPSFPVGY MYPSMDQLAE MLPGVLHKFG LKSVIGMGTG AGAYILTRFA
160 170 180 190 200
LNNPEMVEGL VLMNVNPCAE GWMDWAASKI SGWTQALPDM VVSHLFGKEE
210 220 230 240 250
IHSNVEVVHT YRQHILNDMN PSNLHLFISA YNSRRDLEIE RPMPGTHTVT
260 270 280 290 300
LQCPALLVVG DNSPAVDAVV ECNSKLDPTK TTLLKMADCG GLPQISQPAK
310 320 330 340 350
LAEAFKYFVQ GMGYMPSASM TRLMRSRTAS GSSVTSLEGT RSRSHTSEGP
360 370 380 390
RSRSHTSEGS RSRSHTSEDA RLNITPSSGA TGNNAGPKSM EVSC
Length:394
Mass (Da):42,955
Last modified:July 5, 2004 - v1
Checksum:i42B2B95FBDBC9A16
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY500369 mRNA. Translation: AAS78638.1.
BC081898 mRNA. Translation: AAH81898.1.
RefSeqiNP_001011991.1. NM_001011991.1.
UniGeneiRn.153992.

Genome annotation databases

EnsembliENSRNOT00000010811; ENSRNOP00000010811; ENSRNOG00000007393.
GeneIDi299923.
KEGGirno:299923.
UCSCiRGD:1307303. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY500369 mRNA. Translation: AAS78638.1.
BC081898 mRNA. Translation: AAH81898.1.
RefSeqiNP_001011991.1. NM_001011991.1.
UniGeneiRn.153992.

3D structure databases

ProteinModelPortaliQ6JE36.
SMRiQ6JE36. Positions 31-311.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000010811.

Protein family/group databases

ESTHERiratno-q6je36. Ndr_family.
MEROPSiS33.988.

PTM databases

PhosphoSiteiQ6JE36.

Proteomic databases

PaxDbiQ6JE36.
PRIDEiQ6JE36.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000010811; ENSRNOP00000010811; ENSRNOG00000007393.
GeneIDi299923.
KEGGirno:299923.
UCSCiRGD:1307303. rat.

Organism-specific databases

CTDi10397.
RGDi1307303. Ndrg1.

Phylogenomic databases

eggNOGiNOG310435.
GeneTreeiENSGT00390000001874.
HOGENOMiHOG000230891.
HOVERGENiHBG052591.
InParanoidiQ6JE36.
KOiK18266.
OMAiKTCFNPL.
OrthoDBiEOG7KH9JS.
PhylomeDBiQ6JE36.
TreeFamiTF313168.

Miscellaneous databases

NextBioi645992.
PROiQ6JE36.

Gene expression databases

GenevisibleiQ6JE36. RN.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR004142. NDRG.
IPR030693. NDRG1.
[Graphical view]
PANTHERiPTHR11034. PTHR11034. 1 hit.
PTHR11034:SF18. PTHR11034:SF18. 1 hit.
PfamiPF03096. Ndr. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Wang X., Jing N.
    Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.
  3. Lubec G., Afjehi-Sadat L., Chen W.-Q.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 133-148; 213-234 AND 328-341, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Hippocampus and Spinal cord.

Entry informationi

Entry nameiNDRG1_RAT
AccessioniPrimary (citable) accession number: Q6JE36
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: July 5, 2004
Last modified: June 24, 2015
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.