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Q6JD73 (TPS4A_MAIZE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
7-epi-sesquithujene synthase
EC=4.2.3.101
Alternative name(s):
Terpene synthase 4
Short name=tps4-B73
Gene names
Name:TPS4
OrganismZea mays (Maize)
Taxonomic identifier4577 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › PACMAD clade › Panicoideae › Andropogoneae › Zea

Protein attributes

Sequence length554 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Sesquiterpene synthase involved in the production after herbivore attack of a blend of volatiles that attracts natural enemies of herbivores. Converts farnesyl diphosphate to (S)-beta-bisabolene and 7-epi-sesquithujene, along with a mixture of more than 20 other minor sesquiterpene olefins. Can also act in vitro as a monoterpene synthase, converting geranyl diphosphate to (S)---limonene, beta-myrcene and 11 other monoterpenes. Ref.1

Catalytic activity

(2E,6E)-farnesyl diphosphate = 7-epi-sesquithujene + diphosphate. Ref.1

Cofactor

Magnesium or manganese. Ref.1

Pathway

Secondary metabolite biosynthesis; terpenoid biosynthesis.

Subunit structure

Monomer. Ref.1

Subcellular location

Cytoplasm Probable.

Tissue specificity

Highly expressed in the husk. Detected in leaf sheaths and leaves. Ref.1

Induction

Up-regulated in leaf sheaths after herbivore damage. Ref.1

Domain

The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg2+ By similarity.

Miscellaneous

The allele found in cv. B73 encodes an active enzyme while the allele found in cv. Delprim contains a frame shift mutation (Ref.1).

Sequence similarities

Belongs to the terpene synthase family.

Biophysicochemical properties

Kinetic parameters:

Increased production of (E)-beta-farnesene the presence of manganese.

KM=1.1 µM for farnesyl diphosphate (in the presence of 10 mM magnesium and 1 mM manganese) Ref.1

KM=0.9 µM for geranyl diphosphate (in the presence of 10 mM magnesium and 1 mM manganese)

KM=170 µM for magnesium (in the presence of 10 µM farnesyl diphosphate)

KM=0.5 µM for manganese (in the presence of 10 µM farnesyl diphosphate)

pH dependence:

Optimum pH is 7.0.

Ontologies

Keywords
   Biological processPlant defense
   Cellular componentCytoplasm
   LigandMagnesium
Manganese
Metal-binding
   Molecular functionLyase
Gene Ontology (GO)
   Biological_processdefense response

Inferred from electronic annotation. Source: UniProtKB-KW

terpenoid biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmagnesium ion binding

Inferred from electronic annotation. Source: InterPro

terpene synthase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 5545547-epi-sesquithujene synthase
PRO_0000418849

Regions

Motif308 – 3125DDXXD motif

Sites

Metal binding3081Magnesium or manganese 1 By similarity
Metal binding3081Magnesium or manganese 2 By similarity
Metal binding3121Magnesium or manganese 1 By similarity
Metal binding3121Magnesium or manganese 2 By similarity
Metal binding4521Magnesium or manganese 3 By similarity
Metal binding4561Magnesium or manganese 3 By similarity
Metal binding4601Magnesium or manganese 3 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6JD73 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: EA722D810640E4A1

FASTA55463,879
        10         20         30         40         50         60 
MASPPAHRSS KAADEELPKA SSTFHPSLWG SFFLTYQPPT APQRANMKER AEVLRERVRK 

        70         80         90        100        110        120 
VLKGSTTDQL PETVNLILTL QRLGLGYYYE NEIDKLLHQI YSNSDYNVKD LNLVSQRFYL 

       130        140        150        160        170        180 
LRKNGYDVPS DVFLSFKTEE GGFACAAADT RSLLSLYNAA YLRKHGEEVL DEAISSTRLR 

       190        200        210        220        230        240 
LQDLLGRLLP ESPFAKEVSS SLRTPLFRRV GILEARNYIP IYETEATRNE AVLELAKLNF 

       250        260        270        280        290        300 
NLQQLDFCEE LKHCSAWWNE MIAKSKLTFV RDRIVEEYFW MNGACYDPPY SLSRIILTKI 

       310        320        330        340        350        360 
TGLITIIDDM FDTHGTTEDC MKFAEAFGRW DESAIHLLPE YMKDFYILML ETFQSFEDAL 

       370        380        390        400        410        420 
GPEKSYRVLY LKQAMERLVE LYSKEIKWRD DDYVPTMSEH LQVSAETIAT IALTCSAYAG 

       430        440        450        460        470        480 
MGDMSIRKET FEWALSFPQF IRTFGSFVRL SNDVVSTKRE QTKDHSPSTV HCYMKEHGTT 

       490        500        510        520        530        540 
MDDACEKIKE LIEDSWKDML EQSLALKGLP KVVPQLVFDF SRTTDNMYRD RDALTSSEAL 

       550 
KEMIQLLFVE PIPE

« Hide

References

[1]"The variability of sesquiterpenes emitted from two Zea mays cultivars is controlled by allelic variation of two terpene synthase genes encoding stereoselective multiple product enzymes."
Kollner T.G., Schnee C., Gershenzon J., Degenhardt J.
Plant Cell 16:1115-1131(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, 3D-STRUCTURE MODELING, TISSUE SPECIFICITY, INDUCTION.
Strain: cv. B73.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AY518310 mRNA. Translation: AAS88571.1.

3D structure databases

ProteinModelPortalQ6JD73.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

GrameneQ6JD73.
MaizeGDB1219892.

Phylogenomic databases

HOGENOMHOG000232971.

Enzyme and pathway databases

UniPathwayUPA00213.

Family and domain databases

Gene3D1.10.600.10. 1 hit.
1.50.30.10. 1 hit.
InterProIPR001906. Terpene_synth_N.
IPR005630. Terpene_synthase_metal-bd.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
IPR008949. Terpenoid_synth.
[Graphical view]
PfamPF01397. Terpene_synth. 1 hit.
PF03936. Terpene_synth_C. 1 hit.
[Graphical view]
SUPFAMSSF48239. Terp_cyc_toroid. 1 hit.
SSF48576. Terpenoid_synth. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTPS4A_MAIZE
AccessionPrimary (citable) accession number: Q6JD73
Entry history
Integrated into UniProtKB/Swiss-Prot: September 5, 2012
Last sequence update: July 5, 2004
Last modified: May 1, 2013
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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