Q6J9G1 (STYK1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tyrosine-protein kinase STYK1
EC=2.7.10.2

Alternative name(s):
Novel oncogene with kinase domain
Short name=mNOK
Serine/threonine/tyrosine kinase 1

Gene names

Name:	Styk1
Synonyms:	Nok

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	429 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Probable tyrosine protein-kinase, which has strong transforming capabilities on a variety of cell lines including NIH 3T3 fibroblasts and on athymic nude mice. When overexpressed, it can also induce tumor cell invasion as well as metastasis in distant organs. May act by activating both MAP kinase and phosphatidylinositol 3'-kinases (PI3K) pathways. Ref.1
Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Subcellular location	Membrane; Single-pass membrane protein Potential Ref.1.
Tissue specificity	Highly expressed in colon and small intestine. Weakly or not expressed in spleen, skeletal muscle, liver, kidney, heart and brain. Expressed in transformed kidney cell lines (COS-1 and HEK293T). Ref.1
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Disease	Proto-oncogene
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase Tyrosine-protein kinase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: Compara
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW non-membrane spanning protein tyrosine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q6J9G1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q6J9G1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     67-155: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 429	429	Tyrosine-protein kinase STYK1		PRO_0000088164
Regions
	Transmembrane	30 – 50	21	Helical; Potential
	Domain	119 – 390	272	Protein kinase
	Nucleotide binding	125 – 133	9	ATP By similarity
Sites
	Active site	256	1	Proton acceptor By similarity
	Binding site	152	1	ATP By similarity
Natural variations
	Alternative sequence	67 – 155	89	Missing in isoform 2.		VSP_013427
Experimental info
	Sequence conflict	24	1	R → Q in AAT01225. Ref.1
	Sequence conflict	138	1	M → V Ref.1
	Sequence conflict	140	1	T → A Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified April 26, 2005. Version 2. Checksum: 480130D319551F61	FASTA	429	48,121
	10 20 30 40 50 60 MGEKGHLSRV LLECSLSDKL CVVREKQYEV IIVPALLVGG FLILLAIILW LFIRGQRSQR 70 80 90 100 110 120 QSPGPRGTAS VPASRGRSQE AAGHGEKVLL PLKETSVEGF LRAATPRLAK LQVPREQLLE 130 140 150 160 170 180 VLEQIHSGSC GTLYHATMTT KDHPKPKSVV LKALEDPVGL QEVQDFIGRI QFYQYLGKHK 190 200 210 220 230 240 NLVQLEGCCT ERLPLYMMLE DVVPGDLLSF LWTCRRDVMT MDGLLYDLTE KQIYHIGKQI 250 260 270 280 290 300 LLALEFLQEK HLFHGDVAAR NILIQSDLTP KLCHLGLAYE VHAHGAISSA RSSTIPLKWL 310 320 330 340 350 360 APERLLLRPA SIRGDIWSFG ILLYEMVTLG APPYPEVPPT SILQYLQRKK IMKRPSSCSH 370 380 390 400 410 420 AMYNIMKCCW RWSEDSRPLL GQLLQRLEAA SRSADDKAVL QVPELVVPEL YADVAGIRAE SISYSFSVL
	Isoform 2 [UniParc]. Checksum: 55741BD17D1AB256	FASTA	340	38,585
	10 20 30 40 50 60 MGEKGHLSRV LLECSLSDKL CVVREKQYEV IIVPALLVGG FLILLAIILW LFIRGQRSQR 70 80 90 100 110 120 QSPGPRDPVG LQEVQDFIGR IQFYQYLGKH KNLVQLEGCC TERLPLYMML EDVVPGDLLS 130 140 150 160 170 180 FLWTCRRDVM TMDGLLYDLT EKQIYHIGKQ ILLALEFLQE KHLFHGDVAA RNILIQSDLT 190 200 210 220 230 240 PKLCHLGLAY EVHAHGAISS ARSSTIPLKW LAPERLLLRP ASIRGDIWSF GILLYEMVTL 250 260 270 280 290 300 GAPPYPEVPP TSILQYLQRK KIMKRPSSCS HAMYNIMKCC WRWSEDSRPL LGQLLQRLEA 310 320 330 340 ASRSADDKAV LQVPELVVPE LYADVAGIRA ESISYSFSVL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel protein tyrosine kinase NOK that shares homology with platelet-derived growth factor/fibroblast growth factor receptors induces tumorigenesis and metastasis in nude mice." Liu L., Yu X.-Z., Li T.-S., Song L.-X., Chen P.-L., Suo T.-L., Li Y.-H., Wang S.-D., Chen Y., Ren Y.-M., Zhang S.-P., Chang Z.-J., Fu X.-Y. Cancer Res. 64:3491-3499(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Strain: KM. Tissue: Colon.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6J. Tissue: Cecum and Urinary bladder.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY563053 mRNA. Translation: AAT01225.1. AK033606 mRNA. Translation: BAC28386.1. AK035247 mRNA. Translation: BAC29000.1.
IPI	IPI00227684. IPI00406189.
RefSeq	NP_766479.1. NM_172891.2.
UniGene	Mm.20114.
3D structure databases
ProteinModelPortal	Q6J9G1.
SMR	Q6J9G1. Positions 78-388.
ModBase	Search...
PTM databases
PhosphoSite	Q6J9G1.
Proteomic databases
PaxDb	Q6J9G1.
PRIDE	Q6J9G1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000049150; ENSMUSP00000044098; ENSMUSG00000032899. ENSMUST00000121078; ENSMUSP00000112900; ENSMUSG00000032899.
GeneID	243659.
KEGG	mmu:243659.
UCSC	uc009eir.1. mouse. uc012eub.1. mouse.
Organism-specific databases
CTD	55359.
MGI	MGI:2141396. Styk1.
Phylogenomic databases
eggNOG	COG0515.
GeneTree	ENSGT00530000064033.
HOGENOM	HOG000154447.
HOVERGEN	HBG054067.
InParanoid	Q6J9G1.
KO	K08253.
OMA	SCWRWSE.
OrthoDB	EOG447FTG.
Gene expression databases
Bgee	Q6J9G1.
CleanEx	MM_STYK1.
Genevestigator	Q6J9G1.
GermOnline	ENSMUSG00000032899. Mus musculus.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR001245. Ser-Thr/Tyr_kinase_cat_dom. IPR008266. Tyr_kinase_AS. [Graphical view]
Pfam	PF07714. Pkinase_Tyr. 1 hit. [Graphical view]
PRINTS	PR00109. TYRKINASE.
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	385889.
SOURCE	Search...

Entry information

Entry name

STYK1_MOUSE

Accession

Primary (citable) accession number: Q6J9G1
Secondary accession number(s): Q8BZH6, Q8BZT1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	April 26, 2005
Last modified:	May 1, 2013
This is version 84 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program