Q6J9G0 (STYK1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tyrosine-protein kinase STYK1
EC=2.7.10.2

Alternative name(s):
Novel oncogene with kinase domain
Protein PK-unique
Serine/threonine/tyrosine kinase 1

Gene names

Name:	STYK1
Synonyms:	NOK

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	422 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Probable tyrosine protein-kinase, which has strong transforming capabilities on a variety of cell lines. When overexpressed, it can also induce tumor cell invasion as well as metastasis in distant organs. May act by activating both MAP kinase and phosphatidylinositol 3'-kinases (PI3K) pathways By similarity.
Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Subcellular location	Membrane; Single-pass membrane protein Potential.
Tissue specificity	Widely expressed. Highly expressed in brain, placenta and prostate. Expressed in tumor cells such as hepatoma cells L-02, cervix carcinoma cells HeLa, ovary cancer cells Ho8910 and chronic myelogenous leukemia cells K-562, but not in other tumor cells such as epidermoid carcinoma (A-431). Undetectable in most normal lung tissues, widely expressed in lung cancers. Ref.1 Ref.2 Ref.8
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Polymorphism
Disease	Proto-oncogene
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase Tyrosine-protein kinase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from direct assay. Source: LIFEdb
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW non-membrane spanning protein tyrosine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 422	422	Tyrosine-protein kinase STYK1		PRO_0000088163
Regions
	Transmembrane	26 – 46	21	Helical; Potential
	Domain	114 – 384	271	Protein kinase
	Nucleotide binding	120 – 128	9	ATP By similarity
Sites
	Active site	251	1	Proton acceptor By similarity
	Binding site	147	1	ATP By similarity
Natural variations
	Natural variant	71	1	R → G. Corresponds to variant rs6650202 [ dbSNP | Ensembl ].		VAR_030766
	Natural variant	204	1	S → G. Ref.1 Ref.3 Ref.4 Ref.6 Ref.7 Ref.9 Corresponds to variant rs3759259 [ dbSNP | Ensembl ].		VAR_022245
	Natural variant	210	1	R → W. Ref.9 Corresponds to variant rs34981955 [ dbSNP | Ensembl ].		VAR_041841
	Natural variant	237	1	L → S. Ref.9 Corresponds to variant rs55877878 [ dbSNP | Ensembl ].		VAR_041842
	Natural variant	379	1	R → H. Ref.9 Corresponds to variant rs34638573 [ dbSNP | Ensembl ].		VAR_041843
	Natural variant	395	1	V → I in a glioblastoma multiforme sample; somatic mutation. Ref.9		VAR_041844
	Natural variant	400	1	V → L. Ref.9 Corresponds to variant rs55766125 [ dbSNP | Ensembl ].		VAR_041845
Experimental info
	Sequence conflict	192	1	M → V in AAK34949. Ref.1
	Sequence conflict	192	1	M → V in AAT01226. Ref.2
	Sequence conflict	304	1	S → R in AAK34949. Ref.1
	Sequence conflict	304	1	S → R in AAT01226. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q6J9G0 [UniParc]. Last modified January 11, 2011. Version 4. Checksum: AAD45F481BD61C73	FASTA	422	47,577
	10 20 30 40 50 60 MGMTRMLLEC SLSDKLCVIQ EKQYEVIIVP TLLVTIFLIL LGVILWLFIR EQRTQQQRSG 70 80 90 100 110 120 PQGIAPVPPP RDLSWEAGHG GNVALPLKET SVENFLGATT PALAKLQVPR EQLSEVLEQI 130 140 150 160 170 180 CSGSCGPIFR ANMNTGDPSK PKSVILKALK EPAGLHEVQD FLGRIQFHQY LGKHKNLVQL 190 200 210 220 230 240 EGCCTEKLPL YMVLEDVAQG DLLSFLWTCR RDVMTMDGLL YDLTEKQVYH IGKQVLLALE 250 260 270 280 290 300 FLQEKHLFHG DVAARNILMQ SDLTAKLCGL GLAYEVYTRG AISSTQTIPL KWLAPERLLL 310 320 330 340 350 360 RPASIRADVW SFGILLYEMV TLGAPPYPEV PPTSILEHLQ RRKIMKRPSS CTHTMYSIMK 370 380 390 400 410 420 SCWRWREADR PSPRELRLRL EAAIKTADDE AVLQVPELVV PELYAAVAGI RVESLFYNYS ML

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of a human putative receptor protein kinase cDNA STYK1." Ye X., Ji C., Huang Q., Cheng C., Tang R., Xu J., Zeng L., Dai J., Wu Q., Gu S., Xie Y., Mao Y. Mol. Biol. Rep. 30:91-96(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, VARIANT GLY-204. Tissue: Fetal brain.
[2]	"A novel protein tyrosine kinase NOK that shares homology with platelet-derived growth factor/fibroblast growth factor receptors induces tumorigenesis and metastasis in nude mice." Liu L., Yu X.-Z., Li T.-S., Song L.-X., Chen P.-L., Suo T.-L., Li Y.-H., Wang S.-D., Chen Y., Ren Y.-M., Zhang S.-P., Chang Z.-J., Fu X.-Y. Cancer Res. 64:3491-3499(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Tissue: Amygdala.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT GLY-204. Tissue: Hippocampus.
[4]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT GLY-204. Tissue: Amygdala.
[5]	"The finished DNA sequence of human chromosome 12." Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R. , Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H., Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S., Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H., Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q., Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V., Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E., Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K., Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D., Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R., David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E., D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N., Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N., Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R., Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S., LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H., Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P., Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G., Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E., Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S., Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O., Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J., Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A., Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M., Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I., Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A., Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y., Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A., Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F., Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L., Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G., Gibbs R.A. Nature 440:346-351(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT GLY-204.
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT GLY-204. Tissue: Brain.
[8]	"Diagnostic relevance of overexpressed mRNA of novel oncogene with kinase-domain (NOK) in lung cancers." Amachika T., Kobayashi D., Moriai R., Tsuji N., Watanabe N. Lung Cancer 56:337-340(2007) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[9]	"Patterns of somatic mutation in human cancer genomes." Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R. Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANTS [LARGE SCALE ANALYSIS] GLY-204; TRP-210; SER-237; HIS-379; ILE-395 AND LEU-400.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF251059 mRNA. Translation: AAK34949.1. AY563054 mRNA. Translation: AAT01226.1. AK313906 mRNA. Translation: BAG36629.1. AL353940 mRNA. Translation: CAB89250.1. AC021049 Genomic DNA. No translation available. CH471094 Genomic DNA. Translation: EAW96200.1. BC093822 mRNA. Translation: AAH93822.1. BC093824 mRNA. Translation: AAH93824.1.
IPI	IPI00387030.
PIR	T48680.
RefSeq	NP_060893.2. NM_018423.2.
UniGene	Hs.24979.
3D structure databases
ProteinModelPortal	Q6J9G0.
SMR	Q6J9G0. Positions 88-395.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q6J9G0. 1 interaction.
MINT	MINT-4724498.
STRING	9606.ENSP00000075503.
PTM databases
PhosphoSite	Q6J9G0.
Polymorphism databases
DMDM	126302603.
Proteomic databases
PaxDb	Q6J9G0.
PRIDE	Q6J9G0.
Protocols and materials databases
DNASU	55359.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000075503; ENSP00000075503; ENSG00000060140.
GeneID	55359.
KEGG	hsa:55359.
UCSC	uc001qys.2. human.
Organism-specific databases
CTD	55359.
GeneCards	GC12M010771.
H-InvDB	HIX0201868.
HGNC	HGNC:18889. STYK1.
HPA	HPA048569.
MIM	611433. gene.
neXtProt	NX_Q6J9G0.
PharmGKB	PA134976821.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0515.
HOGENOM	HOG000154447.
HOVERGEN	HBG054067.
InParanoid	Q6J9G0.
KO	K08253.
OMA	SCWRWSE.
OrthoDB	EOG447FTG.
PhylomeDB	Q6J9G0.
Gene expression databases
ArrayExpress	Q6J9G0.
Bgee	Q6J9G0.
CleanEx	HS_STYK1.
Genevestigator	Q6J9G0.
GermOnline	ENSG00000060140. Homo sapiens.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR001245. Ser-Thr/Tyr_kinase_cat_dom. IPR008266. Tyr_kinase_AS. [Graphical view]
Pfam	PF07714. Pkinase_Tyr. 1 hit. [Graphical view]
PRINTS	PR00109. TYRKINASE.
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	55359.
NextBio	59727.
SOURCE	Search...

Entry information

Entry name

STYK1_HUMAN

Accession

Primary (citable) accession number: Q6J9G0
Secondary accession number(s): B2R9T2 , Q52LR3, Q9BXY2, Q9NSH1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	January 11, 2011
Last modified:	May 29, 2013
This is version 94 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.