Reviewed, UniProtKB/Swiss-Prot Q6J9G0 (STYK1_HUMAN)

Names and origin

Protein names

Recommended name:
    Tyrosine-protein kinase STYK1
    EC=2.7.10.2
Alternative name(s):
    Serine/threonine/tyrosine kinase 1
    Novel oncogene with kinase domain
    Protein PK-unique

Gene names

Name:	STYK1
Synonyms:	NOK

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	422 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Probable tyrosine protein-kinase, which has strong transforming capabilities on a variety of cell lines. When overexpressed, it can also induce tumor cell invasion as well as metastasis in distant organs. May act by activating both MAP kinase and phosphatidylinositol 3'-kinases (PI3K) pathways By similarity.
Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Subcellular location	Membrane; Single-pass membrane protein Potential.
Tissue specificity	Widely expressed. Highly expressed in brain, placenta and prostate. Expressed in tumor cells such as hepatoma cells LO2, cervix carcinoma cells HeLa, ovary cancer cells Ho8910 and chronic myelogenous leukemia cells K562, but not in other tumor cells such as epidermoid carcinoma (A431). Undetectable in most normal lung tissues, widely expressed in lung cancers. Ref.1 Ref.2 Ref.5
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Polymorphism
Disease	Proto-oncogene
Domain	Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase Tyrosine-protein kinase
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from direct assay. Source: LIFEdb
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW non-membrane spanning protein tyrosine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 422	422	Tyrosine-protein kinase STYK1		PRO_0000088163
Regions
	Transmembrane	26 – 46	21	Potential
	Domain	114 – 384	271	Protein kinase
	Nucleotide binding	120 – 128	9	ATP By similarity
Sites
	Active site	251	1	Proton acceptor By similarity
	Binding site	147	1	ATP By similarity
Natural variations
	Natural variant	71	1	R → G: dbSNP rs6650202.		VAR_030766
	Natural variant	204	1	G → S: dbSNP rs3759259. Ref.2 Ref.6		VAR_022245
	Natural variant	210	1	R → W Ref.6		VAR_041841
	Natural variant	237	1	L → S Ref.6		VAR_041842
	Natural variant	379	1	R → H Ref.6		VAR_041843
	Natural variant	395	1	V → I in a glioblastoma multiforme sample; somatic mutation. Ref.6		VAR_041844
	Natural variant	400	1	V → L Ref.6		VAR_041845
Experimental info
	Sequence conflict	192	1	M → V in AAK34949. Ref.1
	Sequence conflict	192	1	M → V in AAT01226. Ref.2
	Sequence conflict	304	1	S → R in AAK34949. Ref.1
	Sequence conflict	304	1	S → R in AAT01226. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q6J9G0-1 [UniParc]. Last modified February 20, 2007. Version 3. Checksum: B7CD8BC006029D3B	FASTA	422	47,547
	10 20 30 40 50 60 MGMTRMLLEC SLSDKLCVIQ EKQYEVIIVP TLLVTIFLIL LGVILWLFIR EQRTQQQRSG 70 80 90 100 110 120 PQGIAPVPPP RDLSWEAGHG GNVALPLKET SVENFLGATT PALAKLQVPR EQLSEVLEQI 130 140 150 160 170 180 CSGSCGPIFR ANMNTGDPSK PKSVILKALK EPAGLHEVQD FLGRIQFHQY LGKHKNLVQL 190 200 210 220 230 240 EGCCTEKLPL YMVLEDVAQG DLLGFLWTCR RDVMTMDGLL YDLTEKQVYH IGKQVLLALE 250 260 270 280 290 300 FLQEKHLFHG DVAARNILMQ SDLTAKLCGL GLAYEVYTRG AISSTQTIPL KWLAPERLLL 310 320 330 340 350 360 RPASIRADVW SFGILLYEMV TLGAPPYPEV PPTSILEHLQ RRKIMKRPSS CTHTMYSIMK 370 380 390 400 410 420 SCWRWREADR PSPRELRLRL EAAIKTADDE AVLQVPELVV PELYAAVAGI RVESLFYNYS ML

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of a human putative receptor protein kinase cDNA STYK1." Ye X., Ji C., Huang Q., Cheng C., Tang R., Xu J., Zeng L., Dai J., Wu Q., Gu S., Xie Y., Mao Y. Mol. Biol. Rep. 30:91-96(2003) [PubMed: 12841579] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Tissue: Fetal brain.
[2]	"A novel protein tyrosine kinase NOK that shares homology with platelet-derived growth factor/fibroblast growth factor receptors induces tumorigenesis and metastasis in nude mice." Liu L., Yu X.-Z., Li T.-S., Song L.-X., Chen P.-L., Suo T.-L., Li Y.-H., Wang S.-D., Chen Y., Ren Y.-M., Zhang S.-P., Chang Z.-J., Fu X.-Y. Cancer Res. 64:3491-3499(2004) [PubMed: 15150103] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, VARIANT SER-204. Tissue: Amygdala.
[3]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Blocker H., Heubner D., Hoerlein A., Michel G., Wedler H., Kohrer K., Ottenwalder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Amygdala.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[5]	"Diagnostic relevance of overexpressed mRNA of novel oncogene with kinase-domain (NOK) in lung cancers." Amachika T., Kobayashi D., Moriai R., Tsuji N., Watanabe N. Lung Cancer 56:337-340(2007) [PubMed: 17298854] [Abstract] Cited for: TISSUE SPECIFICITY.
[6]	"Patterns of somatic mutation in human cancer genomes." Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R. Nature 446:153-158(2007) [PubMed: 17344846] [Abstract] Cited for: VARIANTS [LARGE SCALE ANALYSIS] SER-204; TRP-210; SER-237; HIS-379; ILE-395 AND LEU-400.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF251059 mRNA. Translation: AAK34949.1. AY563054 mRNA. Translation: AAT01226.1. AL353940 mRNA. Translation: CAB89250.1. BC093822 mRNA. Translation: AAH93822.1. BC093824 mRNA. Translation: AAH93824.1.
IPI	IPI00387030.
PIR	T48680.
RefSeq	NP_060893.2.
UniGene	Hs.24979
3D structure databases
HSSP	HSSP built from PDB template 1R1W based on UniProtKB P08581.
ModBase	Search...
PTM databases
PhosphoSite	Q6J9G0.
Proteomic databases
PRIDE	Q6J9G0.
Genome annotation databases
Ensembl	ENSG00000060140. Homo sapiens. [Contig view]
GeneID	55359.
KEGG	hsa:55359.
Organism-specific databases
GeneCards	GC12M010666.
H-InvDB	HIX0010428.
HGNC	HGNC:18889. STYK1.
MIM	611433. gene.
PharmGKB	PA134976821.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q6J9G0.
HOVERGEN	Q6J9G0.
Enzyme and pathway databases
BRENDA	2.7.10.2. 247.
Gene expression databases
ArrayExpress	Q6J9G0.
Bgee	Q6J9G0.
CleanEx	HS_STYK1.
GermOnline	ENSG00000060140. Homo sapiens.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR001245. Tyr_pkinase. IPR008266. Tyr_pkinase_AS. [Graphical view]
Pfam	PF07714. Pkinase_Tyr. 1 hit. [Graphical view]
PRINTS	PR00109. TYRKINASE.
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	59727.
SOURCE	Search...

Entry information

Entry name

STYK1_HUMAN

Accession

Primary (citable) accession number: Q6J9G0
Secondary accession number(s): Q52LR3, Q9BXY2, Q9NSH1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	February 20, 2007
Last modified:	June 16, 2009
This is version 55 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)