Reviewed, UniProtKB/Swiss-Prot Q6J5K9 (ARMI_DROME)

Names and origin

Protein names

Recommended name:
    Probable RNA helicase armi
    EC=3.6.1.-
Alternative name(s):
    Armitage protein

Gene names

Name:	armi
ORF Names:	CG11513

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	1274 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Probable RNA helicase required for axial polarization of the oocyte during early and mid oogenesis. Plays a central role in RNA interference (RNAi) process, a process that mediates mRNA destruction of translational repression. Required for the assembly of the RISC complex, a complex required for target RNA destruction or repression. May be required in the RISC assembly to unwind miRNAs, in the production of single-stranded miRNA from the double-stranded miRNA, a key step in RISC formation. Required both for the translational control of oskar (osk) mRNA and cytoskeletal polarization in the oocyte. Ref.1 Ref.4
Subcellular location	Cytoplasm. Ref.1
Tissue specificity	Abundant in oocytes and syncitial blastoderm. Expressed at low level throughout development, including somatic tissues. First apparent early in oogenesis, in the cytoplasm of stem cells and mitotically dividing cystoblasts. In regions 2a and 2b of the germarium, it is most concentrated in the center of the germline cysts, where the pro-oocyte is located. In stage 1 and early stage 2 egg chambers, it accumulates at the anterior of the oocyte, near the ring canals. It also extends through the ring canals forming a branched structure that links the early oocyte with adjacent nurse cells. In stage 3 cysts, it accumulates at the posterior cortex and localizes to extensions that pass through the oocyte into the nurse cells. Through stages 4 to 7, it continues to be somewhat enriched at the posterior cortex of the oocyte, but at significantly lower level. In stage 9 to 10 egg chambers, it is found throughout the cytoplasm of the oocyte and nurse cells, with slight enrichment at the oocyte cortex. Ref.1
Sequence similarities	Belongs to the DNA2/NAM7 helicase family. SDE3 subfamily.

Ontologies

Keywords
Biological process	RNA-mediated gene silencing
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Developmental protein Helicase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	RNA interference Ref.4 Inferred from mutant phenotype. Source: UniProtKB dorsal appendage formation Inferred from mutant phenotype. Source: FlyBase embryonic axis specification Ref.1 Inferred from mutant phenotype. Source: UniProtKB long-term memory Inferred from mutant phenotype. Source: FlyBase microtubule organizing center organization Inferred from mutant phenotype. Source: FlyBase nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Ref.1 Inferred from mutant phenotype. Source: UniProtKB pole plasm protein localization Inferred from mutant phenotype. Source: FlyBase
Cellular component	axon Inferred from direct assay. Source: FlyBase cell soma Inferred from direct assay. Source: FlyBase cytoplasm Ref.1 Inferred from direct assay. Source: UniProtKB dendrite Inferred from direct assay. Source: FlyBase synapse Inferred from direct assay. Source: FlyBase
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA helicase activity Ref.4 Non-traceable author statement. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform A (identifier: Q6J5K9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform B (identifier: Q6J5K9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-86: Missing.
     87-92: DKKAAH → MFTYVS

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1274	1274	Probable RNA helicase armi		PRO_0000080708
Regions
	Nucleotide binding	809 – 816	8	ATP Potential
	Motif	948 – 951	4	DEAG box
Natural variations
	Alternative sequence	1 – 86	86	Missing in isoform B.		VSP_011795
	Alternative sequence	87 – 92	6	DKKAAH → MFTYVS in isoform B.		VSP_011796
Experimental info
	Sequence conflict	608	1	T → K in AAT12000. Ref.1
	Sequence conflict	613	1	I → V in AAT12000. Ref.1
	Sequence conflict	991	1	S → L in AAT12000. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform A [UniParc]. Last modified October 25, 2004. Version 3. Checksum: C340E2B5E4F75653	FASTA	1,274	144,889
	10 20 30 40 50 60 MSSEASGSIT IKEEASTSGT ATNIPVESSE TEAKKPEIGA DKAESQSETT EGGKAAETTE 70 80 90 100 110 120 NHLKRLQNLR KELSYLSETD WMYESLDKKA AHKFFTNPDR NREDILESLD RENSFLDQKL 130 140 150 160 170 180 MEEKMDQQLK ANPNEINGVL SNKIAELTHG LSEMDVSKES SCTARKGVIT SLDGDRGVID 190 200 210 220 230 240 KDVLFETKVA EDIILDLHVG CVVEYLTFTT GEAMRVVKVK SILEHSWEDT SQKEIEKAVD 250 260 270 280 290 300 NLKNEKPTFF NTETRSVLGL ISQRLASSID VETEYGQLTV ELDNIEMNFI PTNGDRVRLE 310 320 330 340 350 360 CNIQLDDGFV DKQGEILEVT KLFPTRIQEG EKCIVERVYV HMVVLGPETY ILKTDLPTGT 370 380 390 400 410 420 DLHLGDIVLA DLIECQYSKF TRRAIKITPL EKNFGATKLT QQSSMGSSGS GKAVTVTGVN 430 440 450 460 470 480 RFITAELWQK ESVSLKLTNN LNRTLRLESI TVCNDSESQL SVVSPLESKE ISSGSEITVT 490 500 510 520 530 540 FEIHTQFLGE AIEKYVLNFD LLKVRRVFTV IVCKTKEEVA EAEKRMIAAE ALMAPGRNSQ 550 560 570 580 590 600 ERSRFYANQV WCNKVDVIPG QQIVTKRRFV ALRLGCFEVP KELRQICLTS ERRQEMIKAI 610 620 630 640 650 660 EQHYSFLTEP LSIKTYMHRF RLLLHLEEIE CFVNFRNYDR DRAHFLRDGE FLTLQIENLA 670 680 690 700 710 720 ERRPSLVIGD TLRVINPWSD PDSQTTKSYE GIIHKVLFDR ILLKFHSSFQ EKYNGEDYRL 730 740 750 760 770 780 EFYFSRYSFR KQHHAISKIV GVMGEDFLFP SKVTKRENPQ LDVYMKDDDM YLYDSKLEWY 790 800 810 820 830 840 NQSLNSIQKR AVFNILRGEA ENIPYVLFGP PGSGKTMTLI ETLLQLVRNL PGARILVGTP 850 860 870 880 890 900 SNSSADLVTK RLIDSKALLQ GDFIRLVSYN QVEKDLIPPE IMSYCATSDV GAVGSCEDKM 910 920 930 940 950 960 MVTESGLKLR CQAKFIGTHR ITISTCTTLG NFLQLGFPAG HFTHVLFDEA GQCTEPETMV 970 980 990 1000 1010 1020 PIVMLTKKRS QVVLSGDPRQ LQSIVTSRIA SKMGFSISFL ERLLERSPYR KDLQRFPESS 1030 1040 1050 1060 1070 1080 GYNPLVLTKL LYNYRALPSI MSIYSRLFYD DELIPVLSEK DSRESRLLSK LRCVFESEKD 1090 1100 1110 1120 1130 1140 IPQAHGTFFY GIIGENRQNN DSPSWFNPQE VREVFLMTIA LYRANVTADQ IGIITPYQKQ 1150 1160 1170 1180 1190 1200 VKMLRSMFIG TDVVMPKIGS VEEFQGQERD IILISTVRSS EEILRMDARF SLGFVRCSKR 1210 1220 1230 1240 1250 1260 LNVAVSRARA MMIIFGNPHL LAVDECWRQL ILFCVKNNAY FGCDLPQMVI NQKDEVPVCL 1270 ETFVPSLNTT DDLN
	Isoform B. Checksum: BA1D8F4305825B0A	FASTA	1,188	135,658
	10 20 30 40 50 60 MFTYVSKFFT NPDRNREDIL ESLDRENSFL DQKLMEEKMD QQLKANPNEI NGVLSNKIAE 70 80 90 100 110 120 LTHGLSEMDV SKESSCTARK GVITSLDGDR GVIDKDVLFE TKVAEDIILD LHVGCVVEYL 130 140 150 160 170 180 TFTTGEAMRV VKVKSILEHS WEDTSQKEIE KAVDNLKNEK PTFFNTETRS VLGLISQRLA 190 200 210 220 230 240 SSIDVETEYG QLTVELDNIE MNFIPTNGDR VRLECNIQLD DGFVDKQGEI LEVTKLFPTR 250 260 270 280 290 300 IQEGEKCIVE RVYVHMVVLG PETYILKTDL PTGTDLHLGD IVLADLIECQ YSKFTRRAIK 310 320 330 340 350 360 ITPLEKNFGA TKLTQQSSMG SSGSGKAVTV TGVNRFITAE LWQKESVSLK LTNNLNRTLR 370 380 390 400 410 420 LESITVCNDS ESQLSVVSPL ESKEISSGSE ITVTFEIHTQ FLGEAIEKYV LNFDLLKVRR 430 440 450 460 470 480 VFTVIVCKTK EEVAEAEKRM IAAEALMAPG RNSQERSRFY ANQVWCNKVD VIPGQQIVTK 490 500 510 520 530 540 RRFVALRLGC FEVPKELRQI CLTSERRQEM IKAIEQHYSF LTEPLSIKTY MHRFRLLLHL 550 560 570 580 590 600 EEIECFVNFR NYDRDRAHFL RDGEFLTLQI ENLAERRPSL VIGDTLRVIN PWSDPDSQTT 610 620 630 640 650 660 KSYEGIIHKV LFDRILLKFH SSFQEKYNGE DYRLEFYFSR YSFRKQHHAI SKIVGVMGED 670 680 690 700 710 720 FLFPSKVTKR ENPQLDVYMK DDDMYLYDSK LEWYNQSLNS IQKRAVFNIL RGEAENIPYV 730 740 750 760 770 780 LFGPPGSGKT MTLIETLLQL VRNLPGARIL VGTPSNSSAD LVTKRLIDSK ALLQGDFIRL 790 800 810 820 830 840 VSYNQVEKDL IPPEIMSYCA TSDVGAVGSC EDKMMVTESG LKLRCQAKFI GTHRITISTC 850 860 870 880 890 900 TTLGNFLQLG FPAGHFTHVL FDEAGQCTEP ETMVPIVMLT KKRSQVVLSG DPRQLQSIVT 910 920 930 940 950 960 SRIASKMGFS ISFLERLLER SPYRKDLQRF PESSGYNPLV LTKLLYNYRA LPSIMSIYSR 970 980 990 1000 1010 1020 LFYDDELIPV LSEKDSRESR LLSKLRCVFE SEKDIPQAHG TFFYGIIGEN RQNNDSPSWF 1030 1040 1050 1060 1070 1080 NPQEVREVFL MTIALYRANV TADQIGIITP YQKQVKMLRS MFIGTDVVMP KIGSVEEFQG 1090 1100 1110 1120 1130 1140 QERDIILIST VRSSEEILRM DARFSLGFVR CSKRLNVAVS RARAMMIIFG NPHLLAVDEC 1150 1160 1170 1180 WRQLILFCVK NNAYFGCDLP QMVINQKDEV PVCLETFVPS LNTTDDLN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Drosophila SDE3 homolog armitage is required for oskar mRNA silencing and embryonic axis specification." Cook H.A., Koppetsch B.S., Wu J., Theurkauf W.E. Cell 116:817-829(2004) [PubMed: 15035984] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Tissue: Ovary.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
[4]	"RISC assembly defects in the Drosophila RNAi mutant armitage." Tomari Y., Du T., Haley B., Schwarz D.S., Bennett R., Cook H.A., Koppetsch B.S., Theurkauf W.E., Zamore P.D. Cell 116:831-841(2004) [PubMed: 15035985] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY598469 mRNA. Translation: AAT12000.1. AE014296 Genomic DNA. Translation: AAF47775.1. AE014296 Genomic DNA. Translation: AAX52729.1.
RefSeq	NP_001014556.1. NP_647816.1.
UniGene	Dm.15753
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	FBgn0041164. Drosophila melanogaster. [Contig view]
GeneID	38427.
KEGG	dme:Dmel_CG11513.
NMPDR	fig|7227.3.peg.7955.
Organism-specific databases
FlyBase	FBgn0041164. armi.
Phylogenomic databases
HOGENOM	Q6J5K9.
OMA	Q6J5K9. FRKQHHA.
Gene expression databases
ArrayExpress	Q6J5K9.
GermOnline	CG11513. Drosophila melanogaster.
Family and domain databases
ProtoNet	Search...
Other Resources
NextBio	808602.

Entry information

Entry name

ARMI_DROME

Accession

Primary (citable) accession number: Q6J5K9
Secondary accession number(s): Q59E56, Q9VZP4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	October 25, 2004
Last modified:	June 16, 2009
This is version 38 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project