Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q6J1Z5

- AMNA_COMTE

UniProt

Q6J1Z5 - AMNA_COMTE

Protein

2-aminophenol 1,6-dioxygenase subunit alpha

Gene

cnbCa

Organism
Comamonas testosteroni (Pseudomonas testosteroni)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 38 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Component of the 2-aminophenol 1,6-dioxygenase (APD) complex that catalyzes the ring fission of 2-aminophenol to produce 2-aminomuconic semialdehyde. CnbCa may have a role in the stability of the complex. The complex is also active on other substrates such as 2-amino-5-chlorophenol (68% activity), protocatechuate (33% activity) and catechol (5% activity). Both 2-aminophenol and 2-amino-5-cholorophenol are likely native substrates for this dioxygenase which is involved in the reductive degradation pathway of both nitrobenzene (NB) and 4-chloronitrobenzene (4-CNB), allowing C.testosteroni strain CNB-1 to grow on these compounds as sole source of carbon, nitrogen, and energy.2 Publications

    Pathwayi

    GO - Molecular functioni

    1. ferrous iron binding Source: InterPro
    2. oxidoreductase activity Source: InterPro

    GO - Biological processi

    1. aromatic compound catabolic process Source: UniProtKB-KW

    Keywords - Biological processi

    Aromatic hydrocarbons catabolism

    Enzyme and pathway databases

    UniPathwayiUPA00923.
    UPA01033.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    2-aminophenol 1,6-dioxygenase subunit alpha1 Publication
    Alternative name(s):
    2-amino-5-chlorophenol 1,6-dioxygenase subunit alphaImported
    Gene namesi
    Name:cnbCaImported
    Synonyms:amnAImported
    Encoded oniPlasmid pCNB14 Publications
    OrganismiComamonas testosteroni (Pseudomonas testosteroni)
    Taxonomic identifieri285 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceaeComamonas

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 2712702-aminophenol 1,6-dioxygenase subunit alpha1 PublicationPRO_0000422780Add
    BLAST

    Interactioni

    Subunit structurei

    The APD complex is a heterotetramer of 2 alpha (CnbCa) and 2 beta (CnbCb) subunits.2 Publications

    Structurei

    Secondary structure

    1
    271
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi3 – 97
    Helixi14 – 174
    Helixi22 – 4019
    Beta strandi44 – 6219
    Beta strandi65 – 717
    Turni74 – 763
    Helixi77 – 793
    Beta strandi82 – 887
    Helixi90 – 10213
    Beta strandi107 – 1104
    Helixi119 – 12810
    Beta strandi131 – 1355
    Beta strandi137 – 1437
    Helixi148 – 16417
    Beta strandi169 – 1746
    Helixi188 – 1903
    Helixi196 – 21116
    Helixi214 – 22714
    Helixi231 – 2344
    Helixi235 – 2439
    Turni244 – 2463
    Beta strandi249 – 25911
    Beta strandi262 – 2709

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3VSGX-ray2.40A/C1-271[»]
    3VSHX-ray2.70A/C1-271[»]
    3VSIX-ray2.50A/C1-271[»]
    3VSJX-ray2.30A/C1-271[»]
    ProteinModelPortaliQ6J1Z5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the LigB/MhpB extradiol dioxygenase family.Sequence Analysis

    Family and domain databases

    Gene3Di3.40.830.10. 1 hit.
    InterProiIPR004183. Xdiol_dOase_suB.
    [Graphical view]
    PfamiPF02900. LigB. 1 hit.
    [Graphical view]
    SUPFAMiSSF53213. SSF53213. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q6J1Z5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTVVSAFLVP GTPLPQLKPE VPSWGQLAAA TERAGKALAA SRPDVVLVYS    50
    TQWLAVLDQQ WLTRPRSEGV HVDENWYEFG DLAYDIRADT ALAEACVTSS 100
    PLHGVHARGV NYDGFPIDTG TITACTLMGI GTDAFPLVVG SNNLYHSGEI 150
    TEKLAALAVD CAKDQNKRVA VVGVGGLSGS LFREEIDPRE DRIANEEDDK 200
    WNRRVLKLIE AGDVSALREA MPVYAKEARV DMGFKHLHWI LGALKGKFSG 250
    ANVLGYGPSY GSGAAVIEFR L 271
    Length:271
    Mass (Da):29,266
    Last modified:July 5, 2004 - v1
    Checksum:i7BECCAF5A68DA7A8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY605054 Genomic DNA. Translation: AAT35227.1.
    EF079106 Genomic DNA. Translation: ABB13578.1.
    RefSeqiYP_001967697.1. NC_010935.1.

    Genome annotation databases

    GeneIDi6386596.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY605054 Genomic DNA. Translation: AAT35227.1 .
    EF079106 Genomic DNA. Translation: ABB13578.1 .
    RefSeqi YP_001967697.1. NC_010935.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3VSG X-ray 2.40 A/C 1-271 [» ]
    3VSH X-ray 2.70 A/C 1-271 [» ]
    3VSI X-ray 2.50 A/C 1-271 [» ]
    3VSJ X-ray 2.30 A/C 1-271 [» ]
    ProteinModelPortali Q6J1Z5.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 6386596.

    Enzyme and pathway databases

    UniPathwayi UPA00923 .
    UPA01033 .

    Family and domain databases

    Gene3Di 3.40.830.10. 1 hit.
    InterProi IPR004183. Xdiol_dOase_suB.
    [Graphical view ]
    Pfami PF02900. LigB. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53213. SSF53213. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "A novel 2-aminophenol 1,6-dioxygenase involved in the degradation of p-chloronitrobenzene by Comamonas strain CNB-1: purification, properties, genetic cloning and expression in Escherichia coli."
      Wu J.F., Sun C.W., Jiang C.Y., Liu Z.P., Liu S.J.
      Arch. Microbiol. 183:1-8(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-9, FUNCTION, SUBUNIT.
      Strain: CNB-11 Publication.
      Plasmid: pCNB1
    2. "Novel partial reductive pathway for 4-chloronitrobenzene and nitrobenzene degradation in Comamonas sp. strain CNB-1."
      Wu J.F., Jiang C.Y., Wang B.J., Ma Y.F., Liu Z.P., Liu S.J.
      Appl. Environ. Microbiol. 72:1759-1765(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, PATHWAY.
      Strain: CNB-11 Publication.
      Plasmid: pCNB1
    3. "Nucleotide sequence of plasmid pCNB1 from Comamonas strain CNB-1 reveals novel genetic organization and evolution for 4-chloronitrobenzene degradation."
      Ma Y.F., Wu J.F., Wang S.Y., Jiang C.Y., Zhang Y., Qi S.W., Liu L., Zhao G.P., Liu S.J.
      Appl. Environ. Microbiol. 73:4477-4483(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PATHWAY.
      Strain: CNB-11 Publication.
      Plasmid: pCNB1
    4. "Structures of aminophenol dioxygenase in complex with intermediate, product and inhibitor."
      Li de F., Zhang J.Y., Hou Y.J., Liu L., Hu Y., Liu S.J., Wang da C., Liu W.
      Acta Crystallogr. D 69:32-43(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS) IN COMPLEX WITH BETA SUBUNIT, SUBUNIT.
      Strain: CNB-11 Publication.
      Plasmid: pCNB1

    Entry informationi

    Entry nameiAMNA_COMTE
    AccessioniPrimary (citable) accession number: Q6J1Z5
    Secondary accession number(s): Q38M40
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 26, 2013
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 38 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Not active on 4-methylcatechol, 4-chlorocatechol, 2,4-dihydroxybenzoate, o-nitrophenol, p-nitrophenol or 4-nitrocatechol.

    Caution

    In contrast to other members of the family, lacks the conserved iron-binding sites, suggesting that the alpha subunit has no oxidoreductase activity.Curated

    Keywords - Technical termi

    3D-structure, Direct protein sequencing, Plasmid

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3