SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q6J1Z5

- AMNA_COMTE

UniProt

Q6J1Z5 - AMNA_COMTE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
2-aminophenol 1,6-dioxygenase subunit alpha
Gene
cnbCa, amnA
Organism
Comamonas testosteroni (Pseudomonas testosteroni)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Component of the 2-aminophenol 1,6-dioxygenase (APD) complex that catalyzes the ring fission of 2-aminophenol to produce 2-aminomuconic semialdehyde. CnbCa may have a role in the stability of the complex. The complex is also active on other substrates such as 2-amino-5-chlorophenol (68% activity), protocatechuate (33% activity) and catechol (5% activity). Both 2-aminophenol and 2-amino-5-cholorophenol are likely native substrates for this dioxygenase which is involved in the reductive degradation pathway of both nitrobenzene (NB) and 4-chloronitrobenzene (4-CNB), allowing C.testosteroni strain CNB-1 to grow on these compounds as sole source of carbon, nitrogen, and energy.3 Publications

Pathwayi

GO - Molecular functioni

  1. ferrous iron binding Source: InterPro
  2. oxidoreductase activity Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. aromatic compound catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Aromatic hydrocarbons catabolism

Enzyme and pathway databases

UniPathwayiUPA00923.
UPA01033.

Names & Taxonomyi

Protein namesi
Recommended name:
2-aminophenol 1,6-dioxygenase subunit alpha
Alternative name(s):
2-amino-5-chlorophenol 1,6-dioxygenase subunit alpha
Gene namesi
Name:cnbCa
Synonyms:amnA
Encoded oniPlasmid pCNB14 Publications
OrganismiComamonas testosteroni (Pseudomonas testosteroni)
Taxonomic identifieri285 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceaeComamonas

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 2712702-aminophenol 1,6-dioxygenase subunit alpha1 Publication
PRO_0000422780Add
BLAST

Interactioni

Subunit structurei

The APD complex is a heterotetramer of 2 alpha (CnbCa) and 2 beta (CnbCb) subunits.2 Publications

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 97
Helixi14 – 174
Helixi22 – 4019
Beta strandi44 – 6219
Beta strandi65 – 717
Turni74 – 763
Helixi77 – 793
Beta strandi82 – 887
Helixi90 – 10213
Beta strandi107 – 1104
Helixi119 – 12810
Beta strandi131 – 1355
Beta strandi137 – 1437
Helixi148 – 16417
Beta strandi169 – 1746
Helixi188 – 1903
Helixi196 – 21116
Helixi214 – 22714
Helixi231 – 2344
Helixi235 – 2439
Turni244 – 2463
Beta strandi249 – 25911
Beta strandi262 – 2709

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3VSGX-ray2.40A/C1-271[»]
3VSHX-ray2.70A/C1-271[»]
3VSIX-ray2.50A/C1-271[»]
3VSJX-ray2.30A/C1-271[»]
ProteinModelPortaliQ6J1Z5.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.830.10. 1 hit.
InterProiIPR004183. Xdiol_dOase_suB.
[Graphical view]
PfamiPF02900. LigB. 1 hit.
[Graphical view]
SUPFAMiSSF53213. SSF53213. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6J1Z5-1 [UniParc]FASTAAdd to Basket

« Hide

MTVVSAFLVP GTPLPQLKPE VPSWGQLAAA TERAGKALAA SRPDVVLVYS    50
TQWLAVLDQQ WLTRPRSEGV HVDENWYEFG DLAYDIRADT ALAEACVTSS 100
PLHGVHARGV NYDGFPIDTG TITACTLMGI GTDAFPLVVG SNNLYHSGEI 150
TEKLAALAVD CAKDQNKRVA VVGVGGLSGS LFREEIDPRE DRIANEEDDK 200
WNRRVLKLIE AGDVSALREA MPVYAKEARV DMGFKHLHWI LGALKGKFSG 250
ANVLGYGPSY GSGAAVIEFR L 271
Length:271
Mass (Da):29,266
Last modified:July 5, 2004 - v1
Checksum:i7BECCAF5A68DA7A8
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY605054 Genomic DNA. Translation: AAT35227.1.
EF079106 Genomic DNA. Translation: ABB13578.1.
RefSeqiYP_001967697.1. NC_010935.1.

Genome annotation databases

GeneIDi6386596.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY605054 Genomic DNA. Translation: AAT35227.1 .
EF079106 Genomic DNA. Translation: ABB13578.1 .
RefSeqi YP_001967697.1. NC_010935.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3VSG X-ray 2.40 A/C 1-271 [» ]
3VSH X-ray 2.70 A/C 1-271 [» ]
3VSI X-ray 2.50 A/C 1-271 [» ]
3VSJ X-ray 2.30 A/C 1-271 [» ]
ProteinModelPortali Q6J1Z5.
ModBasei Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 6386596.

Enzyme and pathway databases

UniPathwayi UPA00923 .
UPA01033 .

Family and domain databases

Gene3Di 3.40.830.10. 1 hit.
InterProi IPR004183. Xdiol_dOase_suB.
[Graphical view ]
Pfami PF02900. LigB. 1 hit.
[Graphical view ]
SUPFAMi SSF53213. SSF53213. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "A novel 2-aminophenol 1,6-dioxygenase involved in the degradation of p-chloronitrobenzene by Comamonas strain CNB-1: purification, properties, genetic cloning and expression in Escherichia coli."
    Wu J.F., Sun C.W., Jiang C.Y., Liu Z.P., Liu S.J.
    Arch. Microbiol. 183:1-8(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-9, FUNCTION, SUBUNIT.
    Strain: CNB-1.
    Plasmid: pCNB1
  2. "Novel partial reductive pathway for 4-chloronitrobenzene and nitrobenzene degradation in Comamonas sp. strain CNB-1."
    Wu J.F., Jiang C.Y., Wang B.J., Ma Y.F., Liu Z.P., Liu S.J.
    Appl. Environ. Microbiol. 72:1759-1765(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, PATHWAY.
    Strain: CNB-1.
    Plasmid: pCNB1
  3. "Nucleotide sequence of plasmid pCNB1 from Comamonas strain CNB-1 reveals novel genetic organization and evolution for 4-chloronitrobenzene degradation."
    Ma Y.F., Wu J.F., Wang S.Y., Jiang C.Y., Zhang Y., Qi S.W., Liu L., Zhao G.P., Liu S.J.
    Appl. Environ. Microbiol. 73:4477-4483(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PATHWAY.
    Strain: CNB-1.
    Plasmid: pCNB1
  4. "Structures of aminophenol dioxygenase in complex with intermediate, product and inhibitor."
    Li de F., Zhang J.Y., Hou Y.J., Liu L., Hu Y., Liu S.J., Wang da C., Liu W.
    Acta Crystallogr. D 69:32-43(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS) IN COMPLEX WITH BETA SUBUNIT, SUBUNIT.
    Strain: CNB-1.
    Plasmid: pCNB1

Entry informationi

Entry nameiAMNA_COMTE
AccessioniPrimary (citable) accession number: Q6J1Z5
Secondary accession number(s): Q38M40
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 26, 2013
Last sequence update: July 5, 2004
Last modified: May 14, 2014
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Not active on 4-methylcatechol, 4-chlorocatechol, 2,4-dihydroxybenzoate, o-nitrophenol, p-nitrophenol or 4-nitrocatechol.1 Publication

Caution

In contrast to other members of the family, lacks the conserved iron-binding sites, suggesting that the alpha subunit has no oxidoreductase activity.

Keywords - Technical termi

3D-structure, Direct protein sequencing, Plasmid

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi