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Q6J1Z5

- AMNA_COMTE

UniProt

Q6J1Z5 - AMNA_COMTE

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Protein

2-aminophenol 1,6-dioxygenase subunit alpha

Gene

cnbCa

Organism
Comamonas testosteroni (Pseudomonas testosteroni)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Component of the 2-aminophenol 1,6-dioxygenase (APD) complex that catalyzes the ring fission of 2-aminophenol to produce 2-aminomuconic semialdehyde. CnbCa may have a role in the stability of the complex. The complex is also active on other substrates such as 2-amino-5-chlorophenol (68% activity), protocatechuate (33% activity) and catechol (5% activity). Both 2-aminophenol and 2-amino-5-cholorophenol are likely native substrates for this dioxygenase which is involved in the reductive degradation pathway of both nitrobenzene (NB) and 4-chloronitrobenzene (4-CNB), allowing C.testosteroni strain CNB-1 to grow on these compounds as sole source of carbon, nitrogen, and energy.2 Publications

Pathwayi

GO - Molecular functioni

  1. ferrous iron binding Source: InterPro
  2. oxidoreductase activity Source: InterPro

GO - Biological processi

  1. aromatic compound catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Aromatic hydrocarbons catabolism

Enzyme and pathway databases

UniPathwayiUPA00923.
UPA01033.

Names & Taxonomyi

Protein namesi
Recommended name:
2-aminophenol 1,6-dioxygenase subunit alpha1 Publication
Alternative name(s):
2-amino-5-chlorophenol 1,6-dioxygenase subunit alphaImported
Gene namesi
Name:cnbCaImported
Synonyms:amnAImported
Encoded oniPlasmid pCNB14 Publications
OrganismiComamonas testosteroni (Pseudomonas testosteroni)
Taxonomic identifieri285 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceaeComamonas

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 2712702-aminophenol 1,6-dioxygenase subunit alpha1 PublicationPRO_0000422780Add
BLAST

Interactioni

Subunit structurei

The APD complex is a heterotetramer of 2 alpha (CnbCa) and 2 beta (CnbCb) subunits.2 Publications

Structurei

Secondary structure

1
271
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 97Combined sources
Helixi14 – 174Combined sources
Helixi22 – 4019Combined sources
Beta strandi44 – 6219Combined sources
Beta strandi65 – 717Combined sources
Turni74 – 763Combined sources
Helixi77 – 793Combined sources
Beta strandi82 – 887Combined sources
Helixi90 – 10213Combined sources
Beta strandi107 – 1104Combined sources
Helixi119 – 12810Combined sources
Beta strandi131 – 1355Combined sources
Beta strandi137 – 1437Combined sources
Helixi148 – 16417Combined sources
Beta strandi169 – 1746Combined sources
Helixi188 – 1903Combined sources
Helixi196 – 21116Combined sources
Helixi214 – 22714Combined sources
Helixi231 – 2344Combined sources
Helixi235 – 2439Combined sources
Turni244 – 2463Combined sources
Beta strandi249 – 25911Combined sources
Beta strandi262 – 2709Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3VSGX-ray2.40A/C1-271[»]
3VSHX-ray2.70A/C1-271[»]
3VSIX-ray2.50A/C1-271[»]
3VSJX-ray2.30A/C1-271[»]
ProteinModelPortaliQ6J1Z5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LigB/MhpB extradiol dioxygenase family.Sequence Analysis

Family and domain databases

Gene3Di3.40.830.10. 1 hit.
InterProiIPR004183. Xdiol_dOase_suB.
[Graphical view]
PfamiPF02900. LigB. 1 hit.
[Graphical view]
SUPFAMiSSF53213. SSF53213. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6J1Z5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTVVSAFLVP GTPLPQLKPE VPSWGQLAAA TERAGKALAA SRPDVVLVYS
60 70 80 90 100
TQWLAVLDQQ WLTRPRSEGV HVDENWYEFG DLAYDIRADT ALAEACVTSS
110 120 130 140 150
PLHGVHARGV NYDGFPIDTG TITACTLMGI GTDAFPLVVG SNNLYHSGEI
160 170 180 190 200
TEKLAALAVD CAKDQNKRVA VVGVGGLSGS LFREEIDPRE DRIANEEDDK
210 220 230 240 250
WNRRVLKLIE AGDVSALREA MPVYAKEARV DMGFKHLHWI LGALKGKFSG
260 270
ANVLGYGPSY GSGAAVIEFR L
Length:271
Mass (Da):29,266
Last modified:July 5, 2004 - v1
Checksum:i7BECCAF5A68DA7A8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY605054 Genomic DNA. Translation: AAT35227.1.
EF079106 Genomic DNA. Translation: ABB13578.1.
RefSeqiYP_001967697.1. NC_010935.1.

Genome annotation databases

GeneIDi6386596.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY605054 Genomic DNA. Translation: AAT35227.1 .
EF079106 Genomic DNA. Translation: ABB13578.1 .
RefSeqi YP_001967697.1. NC_010935.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3VSG X-ray 2.40 A/C 1-271 [» ]
3VSH X-ray 2.70 A/C 1-271 [» ]
3VSI X-ray 2.50 A/C 1-271 [» ]
3VSJ X-ray 2.30 A/C 1-271 [» ]
ProteinModelPortali Q6J1Z5.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 6386596.

Enzyme and pathway databases

UniPathwayi UPA00923 .
UPA01033 .

Family and domain databases

Gene3Di 3.40.830.10. 1 hit.
InterProi IPR004183. Xdiol_dOase_suB.
[Graphical view ]
Pfami PF02900. LigB. 1 hit.
[Graphical view ]
SUPFAMi SSF53213. SSF53213. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "A novel 2-aminophenol 1,6-dioxygenase involved in the degradation of p-chloronitrobenzene by Comamonas strain CNB-1: purification, properties, genetic cloning and expression in Escherichia coli."
    Wu J.F., Sun C.W., Jiang C.Y., Liu Z.P., Liu S.J.
    Arch. Microbiol. 183:1-8(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-9, FUNCTION, SUBUNIT.
    Strain: CNB-11 Publication.
    Plasmid: pCNB1
  2. "Novel partial reductive pathway for 4-chloronitrobenzene and nitrobenzene degradation in Comamonas sp. strain CNB-1."
    Wu J.F., Jiang C.Y., Wang B.J., Ma Y.F., Liu Z.P., Liu S.J.
    Appl. Environ. Microbiol. 72:1759-1765(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, PATHWAY.
    Strain: CNB-11 Publication.
    Plasmid: pCNB1
  3. "Nucleotide sequence of plasmid pCNB1 from Comamonas strain CNB-1 reveals novel genetic organization and evolution for 4-chloronitrobenzene degradation."
    Ma Y.F., Wu J.F., Wang S.Y., Jiang C.Y., Zhang Y., Qi S.W., Liu L., Zhao G.P., Liu S.J.
    Appl. Environ. Microbiol. 73:4477-4483(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PATHWAY.
    Strain: CNB-11 Publication.
    Plasmid: pCNB1
  4. "Structures of aminophenol dioxygenase in complex with intermediate, product and inhibitor."
    Li de F., Zhang J.Y., Hou Y.J., Liu L., Hu Y., Liu S.J., Wang da C., Liu W.
    Acta Crystallogr. D 69:32-43(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS) IN COMPLEX WITH BETA SUBUNIT, SUBUNIT.
    Strain: CNB-11 Publication.
    Plasmid: pCNB1

Entry informationi

Entry nameiAMNA_COMTE
AccessioniPrimary (citable) accession number: Q6J1Z5
Secondary accession number(s): Q38M40
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 26, 2013
Last sequence update: July 5, 2004
Last modified: November 26, 2014
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Not active on 4-methylcatechol, 4-chlorocatechol, 2,4-dihydroxybenzoate, o-nitrophenol, p-nitrophenol or 4-nitrocatechol.

Caution

In contrast to other members of the family, lacks the conserved iron-binding sites, suggesting that the alpha subunit has no oxidoreductase activity.Curated

Keywords - Technical termi

3D-structure, Direct protein sequencing, Plasmid

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3