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Q6J1Z5 (AMNA_COMTE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 37. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
2-aminophenol 1,6-dioxygenase subunit alpha
Alternative name(s):
2-amino-5-chlorophenol 1,6-dioxygenase subunit alpha
Gene names
Name:cnbCa
Synonyms:amnA
Encoded onPlasmid pCNB1 Ref.1 Ref.2 Ref.3 Ref.4
OrganismComamonas testosteroni (Pseudomonas testosteroni)
Taxonomic identifier285 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceaeComamonas

Protein attributes

Sequence length271 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the 2-aminophenol 1,6-dioxygenase (APD) complex that catalyzes the ring fission of 2-aminophenol to produce 2-aminomuconic semialdehyde. CnbCa may have a role in the stability of the complex. The complex is also active on other substrates such as 2-amino-5-chlorophenol (68% activity), protocatechuate (33% activity) and catechol (5% activity). Both 2-aminophenol and 2-amino-5-cholorophenol are likely native substrates for this dioxygenase which is involved in the reductive degradation pathway of both nitrobenzene (NB) and 4-chloronitrobenzene (4-CNB), allowing C.testosteroni strain CNB-1 to grow on these compounds as sole source of carbon, nitrogen, and energy. Ref.1 Ref.2 Ref.4

Pathway

Xenobiotic degradation; nitrobenzene degradation. Ref.2 Ref.3

Xenobiotic degradation; 4-chloronitrobenzene degradation. Ref.2 Ref.3

Subunit structure

The APD complex is a heterotetramer of 2 alpha (CnbCa) and 2 beta (CnbCb) subunits. Ref.1 Ref.4

Miscellaneous

Not active on 4-methylcatechol, 4-chlorocatechol, 2,4-dihydroxybenzoate, o-nitrophenol, p-nitrophenol or 4-nitrocatechol. Ref.1

Sequence similarities

Belongs to the LigB/MhpB extradiol dioxygenase family.

Caution

In contrast to other members of the family, lacks the conserved iron-binding sites, suggesting that the alpha subunit has no oxidoreductase activity.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.1
Chain2 – 2712702-aminophenol 1,6-dioxygenase subunit alpha Ref.1
PRO_0000422780

Secondary structure

............................................ 271
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q6J1Z5 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 7BECCAF5A68DA7A8

FASTA27129,266
        10         20         30         40         50         60 
MTVVSAFLVP GTPLPQLKPE VPSWGQLAAA TERAGKALAA SRPDVVLVYS TQWLAVLDQQ 

        70         80         90        100        110        120 
WLTRPRSEGV HVDENWYEFG DLAYDIRADT ALAEACVTSS PLHGVHARGV NYDGFPIDTG 

       130        140        150        160        170        180 
TITACTLMGI GTDAFPLVVG SNNLYHSGEI TEKLAALAVD CAKDQNKRVA VVGVGGLSGS 

       190        200        210        220        230        240 
LFREEIDPRE DRIANEEDDK WNRRVLKLIE AGDVSALREA MPVYAKEARV DMGFKHLHWI 

       250        260        270 
LGALKGKFSG ANVLGYGPSY GSGAAVIEFR L 

« Hide

References

[1]"A novel 2-aminophenol 1,6-dioxygenase involved in the degradation of p-chloronitrobenzene by Comamonas strain CNB-1: purification, properties, genetic cloning and expression in Escherichia coli."
Wu J.F., Sun C.W., Jiang C.Y., Liu Z.P., Liu S.J.
Arch. Microbiol. 183:1-8(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-9, FUNCTION, SUBUNIT.
Strain: CNB-1.
[2]"Novel partial reductive pathway for 4-chloronitrobenzene and nitrobenzene degradation in Comamonas sp. strain CNB-1."
Wu J.F., Jiang C.Y., Wang B.J., Ma Y.F., Liu Z.P., Liu S.J.
Appl. Environ. Microbiol. 72:1759-1765(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, PATHWAY.
Strain: CNB-1.
[3]"Nucleotide sequence of plasmid pCNB1 from Comamonas strain CNB-1 reveals novel genetic organization and evolution for 4-chloronitrobenzene degradation."
Ma Y.F., Wu J.F., Wang S.Y., Jiang C.Y., Zhang Y., Qi S.W., Liu L., Zhao G.P., Liu S.J.
Appl. Environ. Microbiol. 73:4477-4483(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PATHWAY.
Strain: CNB-1.
[4]"Structures of aminophenol dioxygenase in complex with intermediate, product and inhibitor."
Li de F., Zhang J.Y., Hou Y.J., Liu L., Hu Y., Liu S.J., Wang da C., Liu W.
Acta Crystallogr. D 69:32-43(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS) IN COMPLEX WITH BETA SUBUNIT, SUBUNIT.
Strain: CNB-1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY605054 Genomic DNA. Translation: AAT35227.1.
EF079106 Genomic DNA. Translation: ABB13578.1.
RefSeqYP_001967697.1. NC_010935.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3VSGX-ray2.40A/C1-271[»]
3VSHX-ray2.70A/C1-271[»]
3VSIX-ray2.50A/C1-271[»]
3VSJX-ray2.30A/C1-271[»]
ProteinModelPortalQ6J1Z5.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID6386596.

Enzyme and pathway databases

UniPathwayUPA00923.
UPA01033.

Family and domain databases

Gene3D3.40.830.10. 1 hit.
InterProIPR004183. Xdiol_dOase_suB.
[Graphical view]
PfamPF02900. LigB. 1 hit.
[Graphical view]
SUPFAMSSF53213. SSF53213. 1 hit.
ProtoNetSearch...

Entry information

Entry nameAMNA_COMTE
AccessionPrimary (citable) accession number: Q6J1Z5
Secondary accession number(s): Q38M40
Entry history
Integrated into UniProtKB/Swiss-Prot: June 26, 2013
Last sequence update: July 5, 2004
Last modified: May 14, 2014
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways