Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q6J1Y9

- UBP19_RAT

UniProt

Q6J1Y9 - UBP19_RAT

Protein

Ubiquitin carboxyl-terminal hydrolase 19

Gene

Usp19

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 67 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Deubiquitinating enzyme that regulates the degradation of various proteins. Deubiquitinates and prevents proteasomal degradation of RNF123 which in turn stimulates CDKN1B ubiquitin-dependent degradation thereby playing a role in cell proliferation. Involved in decreased protein synthesis in atrophying skeletal muscle. Modulates transcription of major myofibrillar proteins. Also involved in turnover of endoplasmic-reticulum-associated degradation (ERAD) substrates. Regulates the stability of BIRC2/c-IAP1 and BIRC3/c-IAP2 by preventing thier ubiquitination. Required for cells to mount an appropriate response to hypoxia and rescues HIF1A from degradation in a non-catalytic manner. Plays an important role in 17 beta-estradiol (E2)-inhibited myogenesis. Decreases the levels of ubiquitinated proteins during skeletal muscle formation and acts to repress myogenesis. Exhibits a preference towards 'Lys-63'-linked Ubiquitin chains By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei545 – 5451NucleophilePROSITE-ProRule annotation
    Active sitei1204 – 12041Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri830 – 87243MYND-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. ubiquitin-specific protease activity Source: UniProtKB

    GO - Biological processi

    1. ER-associated ubiquitin-dependent protein catabolic process Source: UniProtKB
    2. negative regulation of skeletal muscle tissue development Source: UniProtKB
    3. positive regulation of cell cycle process Source: UniProtKB
    4. protein deubiquitination Source: UniProtKB
    5. regulation of cellular response to hypoxia Source: UniProtKB
    6. regulation of protein stability Source: UniProtKB
    7. response to endoplasmic reticulum stress Source: UniProtKB
    8. skeletal muscle atrophy Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Keywords - Ligandi

    Metal-binding, Zinc

    Protein family/group databases

    MEROPSiC19.024.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 19 (EC:3.4.19.12)
    Alternative name(s):
    Deubiquitinating enzyme 19
    Ubiquitin thioesterase 19
    Ubiquitin-specific-processing protease 19
    Gene namesi
    Name:Usp19
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi1303276. Usp19.

    Subcellular locationi

    Endoplasmic reticulum membrane 1 Publication; Single-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi545 – 5451C → A: Has a destabilizing effect on RNF123. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 13571357Ubiquitin carboxyl-terminal hydrolase 19PRO_0000295158Add
    BLAST

    Proteomic databases

    PRIDEiQ6J1Y9.

    PTM databases

    PhosphoSiteiQ6J1Y9.

    Expressioni

    Tissue specificityi

    Expressed in testis, heart, kidney and skeletal muscle. Low levels of expression are detectable in all other tissues screened.1 Publication

    Inductioni

    By streptozotocin and fasting in skeletal muscle.1 Publication

    Gene expression databases

    GenevestigatoriQ6J1Y9.

    Interactioni

    Subunit structurei

    Interacts with and stabilizes RNF123. Interacts with BIRC2/c-IAP1, BIRC3/c-IAP2 and XIAP/BIRC4. Interacts with HIF1A (via N-terminus) By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ6J1Y9.
    SMRiQ6J1Y9. Positions 305-441.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 13301330CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini1352 – 13576LumenalSequence Analysis

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei1331 – 135121HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini51 – 14090CS 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini321 – 423103CS 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini536 – 1253718USPAdd
    BLAST

    Sequence similaritiesi

    Contains 2 CS domains.PROSITE-ProRule annotation
    Contains 1 MYND-type zinc finger.PROSITE-ProRule annotation
    Contains 1 USP domain.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri830 – 87243MYND-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Transmembrane, Transmembrane helix, Zinc-finger

    Phylogenomic databases

    HOVERGENiHBG061889.
    KOiK11847.
    PhylomeDBiQ6J1Y9.

    Family and domain databases

    Gene3Di2.60.40.790. 2 hits.
    InterProiIPR007052. CS_dom.
    IPR008978. HSP20-like_chaperone.
    IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    IPR002893. Znf_MYND.
    [Graphical view]
    PfamiPF04969. CS. 2 hits.
    PF00443. UCH. 1 hit.
    PF01753. zf-MYND. 1 hit.
    [Graphical view]
    SUPFAMiSSF49764. SSF49764. 2 hits.
    PROSITEiPS51203. CS. 2 hits.
    PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    PS01360. ZF_MYND_1. 1 hit.
    PS50865. ZF_MYND_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q6J1Y9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSAGTSATGP RRGPPGLEEA TSKKKQKDRA NQESKDGDPR RVSMPRKEPT     50
    KDELLLDWRQ SSDKVVVKLR VGTGPICLEE VDAAFTDTDC VVRLPDGRQW 100
    GGVFFAKIQS SCTKVQTRKG GLLQLALPKK VPLLTWPSLL KKPLGTQELV 150
    PGLRCQENGQ ELSPIALEPG SEPRRAKQEA RNQKRAQGRG EVGSGASPGA 200
    QAGPSAKRAV HLCRGPEGEG SMDGPGPQGD APSFLSDSAT QVEAEEQLHV 250
    PPVNPQTSLL GSEKNLALLT VEKTVSPRSD SVSPVMIRNR DPEKDDHFKE 300
    EMAVGADPAA LADEPESMVN LAFVKNDSYE KGPDSVVVHV YVKESRRDTS 350
    RVLFREQDFT LIFQTRDGNF LRLHPGCGPH TIFRWQVKLR NLIEPEQCTF 400
    CFTASRIDIC LRKRQSQRWG GLEAPATRVG GAKVAVPTGP TPLDSTPPGG 450
    GPLPLTGQEE ARAVEKEKPK ARSEDSGLDG VVARTPLEHV TPKPEPHLAS 500
    PKPTCMVPPM PHSPVSGDSV EEDEEEEKKV CLPGFTGLVN LGNTCFMNSV 550
    IQSLSNTREL RDFFHDRSFE AEINYNNPLG TGGRLAIGFA VLLRALWKGT 600
    HQAFQPSKLK AIVASKASQF TGYAQHDAQE FMAFLLDGLH EDLNRIQNKP 650
    YTETVDSDGR PDEVVAEEAW QRHKMRNDSF IVDLFQGQYK SKLVCPVCAK 700
    VSITFDPFLY LPVPLPQKQK VLPIYYFARE PHSKPIKFLV SVSKENSSAS 750
    EVLESLSQSV HVKPESLRLA EVIKNRFHRV FLPSHSLDAV SPTDVLLCFE 800
    LLSPELAKER VVVLEVQQRP QVPSIPISKC AACQRKQQSE DEKLKRCTRC 850
    YRVGYCNQFC QKTHWPDHKG LCRPENIGYP FLVSVPASRL TYARLAQLLE 900
    GYARYSVSVF QPPFQPGRMA LESQSPGCTT LLSTSSLEAG DSEREPIQPS 950
    ELQLVTPVAE GDTGAHRMWP PADRGPVPST SGISSEMLAS GPMEGCSLLA 1000
    GERVSRPEAA VPGYQHSRES VSAHTPQFFI YKIDASSREQ RLEDKGDTPL 1050
    ELGDDCSLAL VWRNNERLQE FVLVASKELE CAEDPGSAGE AARAGHFTLD 1100
    QCLNLFTRPE VLAPEEAWYC PQCKQHREAS KQLLLWRLPN VLIVQLKRFS 1150
    FRSFIWRDKI NDLVEFPVRN LDLSKFCIGQ KEEQLPSYDL YAVINHYGGM 1200
    IGGHYTACAR LPSDRSSQRS DVGWRLFDDS TVTTVDESQV VTRYAYVLFY 1250
    RRRNSPVERP PRAAHAEHHP DLGPAAEAAA SQASRIWQEL EAEEEMVPEG 1300
    PGPLGPWGPQ DWVGPPPRGP TTSDEGCLRY FVLGTVAALV ALVLNVFYPL 1350
    VSQSRWR 1357
    Length:1,357
    Mass (Da):150,302
    Last modified:July 5, 2004 - v1
    Checksum:iE2CE888D61EC3C81
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY605065 mRNA. Translation: AAT35219.1.
    RefSeqiNP_001001516.1. NM_001001516.2.
    UniGeneiRn.13484.

    Genome annotation databases

    GeneIDi361190.
    KEGGirno:361190.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY605065 mRNA. Translation: AAT35219.1 .
    RefSeqi NP_001001516.1. NM_001001516.2.
    UniGenei Rn.13484.

    3D structure databases

    ProteinModelPortali Q6J1Y9.
    SMRi Q6J1Y9. Positions 305-441.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi C19.024.

    PTM databases

    PhosphoSitei Q6J1Y9.

    Proteomic databases

    PRIDEi Q6J1Y9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 361190.
    KEGGi rno:361190.

    Organism-specific databases

    CTDi 10869.
    RGDi 1303276. Usp19.

    Phylogenomic databases

    HOVERGENi HBG061889.
    KOi K11847.
    PhylomeDBi Q6J1Y9.

    Miscellaneous databases

    NextBioi 675485.
    PROi Q6J1Y9.

    Gene expression databases

    Genevestigatori Q6J1Y9.

    Family and domain databases

    Gene3Di 2.60.40.790. 2 hits.
    InterProi IPR007052. CS_dom.
    IPR008978. HSP20-like_chaperone.
    IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    IPR002893. Znf_MYND.
    [Graphical view ]
    Pfami PF04969. CS. 2 hits.
    PF00443. UCH. 1 hit.
    PF01753. zf-MYND. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49764. SSF49764. 2 hits.
    PROSITEi PS51203. CS. 2 hits.
    PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    PS01360. ZF_MYND_1. 1 hit.
    PS50865. ZF_MYND_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "USP19 is a ubiquitin-specific protease regulated in rat skeletal muscle during catabolic states."
      Combaret L., Adegoke O.A.J., Bedard N., Baracos V., Attaix D., Wing S.S.
      Am. J. Physiol. 288:E693-E700(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION.
      Strain: Sprague-Dawley.
      Tissue: Testis.
    2. Lubec G., Kang S.U., Lubec S.
      Submitted (SEP-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 2-11; 60-76 AND 837-845, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Sprague-Dawley.
      Tissue: Brain.
    3. "USP19-deubiquitinating enzyme regulates levels of major myofibrillar proteins in L6 muscle cells."
      Sundaram P., Pang Z., Miao M., Yu L., Wing S.S.
      Am. J. Physiol. 297:E1283-E1290(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    4. "USP19 deubiquitinating enzyme supports cell proliferation by stabilizing KPC1, a ubiquitin ligase for p27Kip1."
      Lu Y., Adegoke O.A.J., Nepveu A., Nakayama K.I., Bedard N., Cheng D., Peng J., Wing S.S.
      Mol. Cell. Biol. 29:547-558(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH RNF123, MUTAGENESIS OF CYS-545.

    Entry informationi

    Entry nameiUBP19_RAT
    AccessioniPrimary (citable) accession number: Q6J1Y9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 10, 2007
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 67 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3