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Q6IWH7

- ANO7_HUMAN

UniProt

Q6IWH7 - ANO7_HUMAN

Protein

Anoctamin-7

Gene

ANO7

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 91 (01 Oct 2014)
      Sequence version 2 (31 May 2011)
      Previous versions | rss
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    Functioni

    Does not exhibit calcium-activated chloride channel (CaCC) activity. May play a role in cell-cell interactions.1 Publication

    GO - Molecular functioni

    1. intracellular calcium activated chloride channel activity Source: UniProt

    GO - Biological processi

    1. chloride transmembrane transport Source: GOC
    2. chloride transport Source: UniProt
    3. ion transmembrane transport Source: Reactome
    4. transmembrane transport Source: Reactome

    Enzyme and pathway databases

    ReactomeiREACT_160189. Stimuli-sensing channels.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Anoctamin-7
    Alternative name(s):
    Dresden transmembrane protein of the prostate
    Short name:
    D-TMPP
    IPCA-5
    New gene expressed in prostate
    Prostate cancer-associated protein 5
    Transmembrane protein 16G
    Gene namesi
    Name:ANO7
    Synonyms:NGEP, PCANAP5, TMEM16G
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 2

    Organism-specific databases

    HGNCiHGNC:31677. ANO7.

    Subcellular locationi

    Isoform 1 : Cell membrane; Multi-pass membrane protein. Cell junction. Endoplasmic reticulum
    Note: Concentrates at sites of cell-cell contact. Shows an intracellular localization according to PubMed:22075693 and PubMed:20056604.

    GO - Cellular componenti

    1. cell junction Source: UniProtKB-SubCell
    2. cytosol Source: UniProtKB-SubCell
    3. endoplasmic reticulum Source: UniProtKB
    4. integral component of membrane Source: UniProtKB-KW
    5. intracellular Source: UniProtKB
    6. nucleus Source: Ensembl
    7. plasma membrane Source: UniProt

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Cytoplasm, Endoplasmic reticulum, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA32980.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 933933Anoctamin-7PRO_0000289326Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi809 – 8091N-linked (GlcNAc...)1 Publication
    Glycosylationi824 – 8241N-linked (GlcNAc...)1 Publication

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ6IWH7.
    PRIDEiQ6IWH7.

    Expressioni

    Tissue specificityi

    Specifically expressed in epithelial cells of the prostate (at protein level).3 Publications

    Inductioni

    Up-regulated by androgen.1 Publication

    Gene expression databases

    ArrayExpressiQ6IWH7.
    BgeeiQ6IWH7.
    CleanExiHS_ANO7.
    GenevestigatoriQ6IWH7.

    Organism-specific databases

    HPAiHPA035730.
    HPA035731.

    Interactioni

    Protein-protein interaction databases

    STRINGi9606.ENSP00000274979.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6IWH7.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 355355CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini377 – 42044ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini442 – 49958CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini521 – 55030ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini572 – 58817CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini610 – 714105ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini736 – 76328CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini785 – 84359ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini865 – 93369CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei356 – 37621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei421 – 44121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei500 – 52021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei551 – 57121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei589 – 60921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei715 – 73521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei764 – 78421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei844 – 86421HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the anoctamin family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG320103.
    HOGENOMiHOG000006509.
    HOVERGENiHBG069519.
    InParanoidiQ6IWH7.
    OMAiCAYMEML.
    OrthoDBiEOG7BS48W.
    PhylomeDBiQ6IWH7.
    TreeFamiTF314265.

    Family and domain databases

    InterProiIPR007632. Anoctamin.
    [Graphical view]
    PANTHERiPTHR12308. PTHR12308. 1 hit.
    PfamiPF04547. Anoctamin. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q6IWH7-1) [UniParc]FASTAAdd to Basket

    Also known as: NGEP-L

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MRMAATAWAG LQGPPLPTLC PAVRTGLYCR DQAHAERWAM TSETSSGSHC    50
    ARSRMLRRRA QEEDSTVLID VSPPEAEKRG SYGSTAHASE PGGQQAAACR 100
    AGSPAKPRIA DFVLVWEEDL KLDRQQDSAA RDRTDMHRTW RETFLDNLRA 150
    AGLCVDQQDV QDGNTTVHYA LLSASWAVLC YYAEDLRLKL PLQELPNQAS 200
    NWSAGLLAWL GIPNVLLEVV PDVPPEYYSC RFRVNKLPRF LGSDNQDTFF 250
    TSTKRHQILF EILAKTPYGH EKKNLLGIHQ LLAEGVLSAA FPLHDGPFKT 300
    PPEGPQAPRL NQRQVLFQHW ARWGKWNKYQ PLDHVRRYFG EKVALYFAWL 350
    GFYTGWLLPA AVVGTLVFLV GCFLVFSDIP TQELCGSKDS FEMCPLCLDC 400
    PFWLLSSACA LAQAGRLFDH GGTVFFSLFM ALWAVLLLEY WKRKSATLAY 450
    RWDCSDYEDT EERPRPQFAA SAPMTAPNPI TGEDEPYFPE RSRARRMLAG 500
    SVVIVVMVAV VVMCLVSIIL YRAIMAIVVS RSGNTLLAAW ASRIASLTGS 550
    VVNLVFILIL SKIYVSLAHV LTRWEMHRTQ TKFEDAFTLK VFIFQFVNFY 600
    SSPVYIAFFK GRFVGYPGNY HTLFGVRNEE CAAGGCLIEL AQELLVIMVG 650
    KQVINNMQEV LIPKLKGWWQ KFRLRSKKRK AGASAGASQG PWEDDYELVP 700
    CEGLFDEYLE MVLQFGFVTI FVAACPLAPL FALLNNWVEI RLDARKFVCE 750
    YRRPVAERAQ DIGIWFHILA GLTHLAVISN AFLLAFSSDF LPRAYYRWTR 800
    AHDLRGFLNF TLARAPSSFA AAHNRTCRYR AFRDDDGHYS QTYWNLLAIR 850
    LAFVIVFEHV VFSVGRLLDL LVPDIPESVE IKVKREYYLA KQALAENEVL 900
    FGTNGTKDEQ PEGSELSSHW TPFTVPKASQ LQQ 933
    Length:933
    Mass (Da):105,532
    Last modified:May 31, 2011 - v2
    Checksum:iD6FD42578A4BD773
    GO
    Isoform 2 (identifier: Q6IWH7-2) [UniParc]FASTAAdd to Basket

    Also known as: NGEP-S

    The sequence of this isoform differs from the canonical sequence as follows:
         110-110: Missing.
         158-180: QDVQDGNTTVHYALLSASWAVLC → VRGGCHGQGPRPCIHSVTHDLAA
         181-933: Missing.

    Show »
    Length:179
    Mass (Da):19,590
    Checksum:iA300C8733A94485B
    GO
    Isoform 3 (identifier: Q6IWH7-3) [UniParc]FASTAAdd to Basket

    Also known as: D-TMPP

    The sequence of this isoform differs from the canonical sequence as follows:
         110-110: Missing.
         859-859: H → VAEAPAGSPIHGMRPRPCALPNSSTW
         860-933: Missing.

    Show »
    Length:883
    Mass (Da):99,754
    Checksum:iA91DCD1079F7AB64
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti67 – 671V → I.
    Corresponds to variant rs2302054 [ dbSNP | Ensembl ].
    VAR_032616
    Natural varianti912 – 9121E → K.1 Publication
    Corresponds to variant rs7590653 [ dbSNP | Ensembl ].
    VAR_065166

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei110 – 1101Missing in isoform 2 and isoform 3. 1 PublicationVSP_026004
    Alternative sequencei158 – 18023QDVQD…WAVLC → VRGGCHGQGPRPCIHSVTHD LAA in isoform 2. 1 PublicationVSP_026005Add
    BLAST
    Alternative sequencei181 – 933753Missing in isoform 2. 1 PublicationVSP_026006Add
    BLAST
    Alternative sequencei859 – 8591H → VAEAPAGSPIHGMRPRPCAL PNSSTW in isoform 3. CuratedVSP_026007
    Alternative sequencei860 – 93374Missing in isoform 3. CuratedVSP_026008Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY617079 mRNA. Translation: AAT40139.1.
    AY617080 mRNA. Translation: AAT40140.1.
    AC005237 Genomic DNA. No translation available.
    AC104841 Genomic DNA. No translation available.
    AC111201 Genomic DNA. No translation available.
    CCDSiCCDS33423.1. [Q6IWH7-1]
    CCDS46563.1. [Q6IWH7-2]
    RefSeqiNP_001001666.1. NM_001001666.3. [Q6IWH7-2]
    NP_001001891.2. NM_001001891.3. [Q6IWH7-1]
    UniGeneiHs.163909.

    Genome annotation databases

    EnsembliENST00000274979; ENSP00000274979; ENSG00000146205. [Q6IWH7-1]
    ENST00000402430; ENSP00000385418; ENSG00000146205. [Q6IWH7-3]
    ENST00000402530; ENSP00000383985; ENSG00000146205. [Q6IWH7-2]
    GeneIDi50636.
    KEGGihsa:50636.
    UCSCiuc002waw.3. human. [Q6IWH7-2]
    uc002wax.2. human. [Q6IWH7-1]

    Polymorphism databases

    DMDMi334302764.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY617079 mRNA. Translation: AAT40139.1 .
    AY617080 mRNA. Translation: AAT40140.1 .
    AC005237 Genomic DNA. No translation available.
    AC104841 Genomic DNA. No translation available.
    AC111201 Genomic DNA. No translation available.
    CCDSi CCDS33423.1. [Q6IWH7-1 ]
    CCDS46563.1. [Q6IWH7-2 ]
    RefSeqi NP_001001666.1. NM_001001666.3. [Q6IWH7-2 ]
    NP_001001891.2. NM_001001891.3. [Q6IWH7-1 ]
    UniGenei Hs.163909.

    3D structure databases

    ProteinModelPortali Q6IWH7.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9606.ENSP00000274979.

    Polymorphism databases

    DMDMi 334302764.

    Proteomic databases

    PaxDbi Q6IWH7.
    PRIDEi Q6IWH7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000274979 ; ENSP00000274979 ; ENSG00000146205 . [Q6IWH7-1 ]
    ENST00000402430 ; ENSP00000385418 ; ENSG00000146205 . [Q6IWH7-3 ]
    ENST00000402530 ; ENSP00000383985 ; ENSG00000146205 . [Q6IWH7-2 ]
    GeneIDi 50636.
    KEGGi hsa:50636.
    UCSCi uc002waw.3. human. [Q6IWH7-2 ]
    uc002wax.2. human. [Q6IWH7-1 ]

    Organism-specific databases

    CTDi 50636.
    GeneCardsi GC02P242127.
    HGNCi HGNC:31677. ANO7.
    HPAi HPA035730.
    HPA035731.
    MIMi 605096. gene.
    neXtProti NX_Q6IWH7.
    PharmGKBi PA32980.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG320103.
    HOGENOMi HOG000006509.
    HOVERGENi HBG069519.
    InParanoidi Q6IWH7.
    OMAi CAYMEML.
    OrthoDBi EOG7BS48W.
    PhylomeDBi Q6IWH7.
    TreeFami TF314265.

    Enzyme and pathway databases

    Reactomei REACT_160189. Stimuli-sensing channels.

    Miscellaneous databases

    GenomeRNAii 50636.
    NextBioi 53174.
    PROi Q6IWH7.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q6IWH7.
    Bgeei Q6IWH7.
    CleanExi HS_ANO7.
    Genevestigatori Q6IWH7.

    Family and domain databases

    InterProi IPR007632. Anoctamin.
    [Graphical view ]
    PANTHERi PTHR12308. PTHR12308. 1 hit.
    Pfami PF04547. Anoctamin. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "NGEP, a gene encoding a membrane protein detected only in prostate cancer and normal prostate."
      Bera T.K., Das S., Maeda H., Beers R., Wolfgang C.D., Kumar V., Hahn Y., Lee B., Pastan I.
      Proc. Natl. Acad. Sci. U.S.A. 101:3059-3064(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), VARIANT LYS-912, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, TOPOLOGY.
    2. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
      Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
      , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
      Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "Prediction of gene function by genome-scale expression analysis: prostate cancer-associated genes."
      Walker M.G., Volkmuth W., Sprinzak E., Hodgson D., Klingler T.
      Genome Res. 9:1198-1203(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION.
    4. "Characterization of human TMEM16G gene in silico."
      Katoh M., Katoh M.
      Int. J. Mol. Med. 14:759-764(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION.
    5. "D-TMPP: a novel androgen-regulated gene preferentially expressed in prostate and prostate cancer that is the first characterized member of an eukaryotic gene family."
      Kiessling A., Weigle B., Fuessel S., Ebner R., Meye A., Rieger M.A., Schmitz M., Temme A., Bachmann M., Wirth M.P., Rieber E.P.
      Prostate 64:387-400(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: ALTERNATIVE SPLICING (ISOFORM 3), TISSUE SPECIFICITY, INDUCTION BY ANDROGEN.
    6. "NGEP, a prostate-specific plasma membrane protein that promotes the association of LNCaP cells."
      Das S., Hahn Y., Nagata S., Willingham M.C., Bera T.K., Lee B., Pastan I.
      Cancer Res. 67:1594-1601(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    7. "Topology of NGEP, a prostate-specific cell:cell junction protein widely expressed in many cancers of different grade level."
      Das S., Hahn Y., Walker D.A., Nagata S., Willingham M.C., Peehl D.M., Bera T.K., Lee B., Pastan I.
      Cancer Res. 68:6306-6312(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION AT ASN-809 AND ASN-824, MEMBRANE TOPOLOGY.
    8. Cited for: SUBCELLULAR LOCATION.
    9. "Physiological roles and diseases of Tmem16/Anoctamin proteins: are they all chloride channels?"
      Duran C., Hartzell H.C.
      Acta Pharmacol. Sin. 32:685-692(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.
    10. Cited for: REVIEW.
    11. "ANOs 3-7 in the anoctamin/Tmem16 Cl- channel family are intracellular proteins."
      Duran C., Qu Z., Osunkoya A.O., Cui Y., Hartzell H.C.
      Am. J. Physiol. 302:C482-C493(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: ABSENCE OF CALCIUM-ACTIVATED CHLORIDE CHANNEL ACTIVITY, SUBCELLULAR LOCATION.
    12. "The anoctamin (TMEM16) gene family: calcium-activated chloride channels come of age."
      Winpenny J.P., Gray M.A.
      Exp. Physiol. 97:175-176(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.
    13. "The anoctamin family: TMEM16A and TMEM16B as calcium-activated chloride channels."
      Scudieri P., Sondo E., Ferrera L., Galietta L.J.
      Exp. Physiol. 97:177-183(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW, ABSENCE OF CALCIUM-ACTIVATED CHLORIDE CHANNEL ACTIVITY.
    14. "Anoctamins are a family of Ca2+ activated Cl- channels."
      Tian Y., Schreiber R., Kunzelmann K.
      J. Cell Sci. 125:4991-4998(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiANO7_HUMAN
    AccessioniPrimary (citable) accession number: Q6IWH7
    Secondary accession number(s): Q6IWH6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 29, 2007
    Last sequence update: May 31, 2011
    Last modified: October 1, 2014
    This is version 91 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Miscellaneous

    The term 'anoctamin' was coined because these channels are anion selective and have eight (OCT) transmembrane segments. There is some dissatisfaction in the field with the Ano nomenclature because it is not certain that all the members of this family are anion channels or have the 8-transmembrane topology.

    Caution

    It is uncertain whether Met-1 or Met-55 is the initiator.Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 2
      Human chromosome 2: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3