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Protein

Anoctamin-7

Gene

ANO7

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has calcium-dependent phospholipid scramblase activity; scrambles phosphatidylserine, phosphatidylcholine and galactosylceramide (By similarity). Does not exhibit calcium-activated chloride channel (CaCC) activity. May play a role in cell-cell interactions.By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Lipid transport, Transport

Enzyme and pathway databases

ReactomeiREACT_160189. Stimuli-sensing channels.

Names & Taxonomyi

Protein namesi
Recommended name:
Anoctamin-7
Alternative name(s):
Dresden transmembrane protein of the prostate
Short name:
D-TMPP
IPCA-5
New gene expressed in prostate
Prostate cancer-associated protein 5
Transmembrane protein 16G
Gene namesi
Name:ANO7
Synonyms:NGEP, PCANAP5, TMEM16G
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:31677. ANO7.

Subcellular locationi

Isoform 1 :

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 355355CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei356 – 37621HelicalSequence AnalysisAdd
BLAST
Topological domaini377 – 42044ExtracellularSequence AnalysisAdd
BLAST
Transmembranei421 – 44121HelicalSequence AnalysisAdd
BLAST
Topological domaini442 – 49958CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei500 – 52021HelicalSequence AnalysisAdd
BLAST
Topological domaini521 – 55030ExtracellularSequence AnalysisAdd
BLAST
Transmembranei551 – 57121HelicalSequence AnalysisAdd
BLAST
Topological domaini572 – 58817CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei589 – 60921HelicalSequence AnalysisAdd
BLAST
Topological domaini610 – 714105ExtracellularSequence AnalysisAdd
BLAST
Transmembranei715 – 73521HelicalSequence AnalysisAdd
BLAST
Topological domaini736 – 76328CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei764 – 78421HelicalSequence AnalysisAdd
BLAST
Topological domaini785 – 84359ExtracellularSequence AnalysisAdd
BLAST
Transmembranei844 – 86421HelicalSequence AnalysisAdd
BLAST
Topological domaini865 – 93369CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • cell junction Source: UniProtKB-SubCell
  • cytosol Source: UniProtKB-SubCell
  • endoplasmic reticulum Source: UniProtKB
  • integral component of membrane Source: UniProtKB-KW
  • intracellular Source: UniProtKB
  • nucleus Source: Ensembl
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Endoplasmic reticulum, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA32980.

Polymorphism and mutation databases

BioMutaiANO7.
DMDMi334302764.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 933933Anoctamin-7PRO_0000289326Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi809 – 8091N-linked (GlcNAc...)1 Publication
Glycosylationi824 – 8241N-linked (GlcNAc...)1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ6IWH7.
PRIDEiQ6IWH7.

Expressioni

Tissue specificityi

Specifically expressed in epithelial cells of the prostate (at protein level).3 Publications

Inductioni

Up-regulated by androgen.1 Publication

Gene expression databases

BgeeiQ6IWH7.
CleanExiHS_ANO7.
ExpressionAtlasiQ6IWH7. baseline and differential.
GenevisibleiQ6IWH7. HS.

Organism-specific databases

HPAiHPA035730.
HPA035731.

Interactioni

Protein-protein interaction databases

STRINGi9606.ENSP00000274979.

Structurei

3D structure databases

ProteinModelPortaliQ6IWH7.
SMRiQ6IWH7. Positions 332-792.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the anoctamin family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG320103.
GeneTreeiENSGT00760000119015.
HOGENOMiHOG000006509.
HOVERGENiHBG069519.
InParanoidiQ6IWH7.
OMAiILAKTPY.
OrthoDBiEOG7BS48W.
PhylomeDBiQ6IWH7.
TreeFamiTF314265.

Family and domain databases

InterProiIPR007632. Anoctamin.
[Graphical view]
PANTHERiPTHR12308. PTHR12308. 1 hit.
PfamiPF04547. Anoctamin. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6IWH7-1) [UniParc]FASTAAdd to basket

Also known as: NGEP-L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRMAATAWAG LQGPPLPTLC PAVRTGLYCR DQAHAERWAM TSETSSGSHC
60 70 80 90 100
ARSRMLRRRA QEEDSTVLID VSPPEAEKRG SYGSTAHASE PGGQQAAACR
110 120 130 140 150
AGSPAKPRIA DFVLVWEEDL KLDRQQDSAA RDRTDMHRTW RETFLDNLRA
160 170 180 190 200
AGLCVDQQDV QDGNTTVHYA LLSASWAVLC YYAEDLRLKL PLQELPNQAS
210 220 230 240 250
NWSAGLLAWL GIPNVLLEVV PDVPPEYYSC RFRVNKLPRF LGSDNQDTFF
260 270 280 290 300
TSTKRHQILF EILAKTPYGH EKKNLLGIHQ LLAEGVLSAA FPLHDGPFKT
310 320 330 340 350
PPEGPQAPRL NQRQVLFQHW ARWGKWNKYQ PLDHVRRYFG EKVALYFAWL
360 370 380 390 400
GFYTGWLLPA AVVGTLVFLV GCFLVFSDIP TQELCGSKDS FEMCPLCLDC
410 420 430 440 450
PFWLLSSACA LAQAGRLFDH GGTVFFSLFM ALWAVLLLEY WKRKSATLAY
460 470 480 490 500
RWDCSDYEDT EERPRPQFAA SAPMTAPNPI TGEDEPYFPE RSRARRMLAG
510 520 530 540 550
SVVIVVMVAV VVMCLVSIIL YRAIMAIVVS RSGNTLLAAW ASRIASLTGS
560 570 580 590 600
VVNLVFILIL SKIYVSLAHV LTRWEMHRTQ TKFEDAFTLK VFIFQFVNFY
610 620 630 640 650
SSPVYIAFFK GRFVGYPGNY HTLFGVRNEE CAAGGCLIEL AQELLVIMVG
660 670 680 690 700
KQVINNMQEV LIPKLKGWWQ KFRLRSKKRK AGASAGASQG PWEDDYELVP
710 720 730 740 750
CEGLFDEYLE MVLQFGFVTI FVAACPLAPL FALLNNWVEI RLDARKFVCE
760 770 780 790 800
YRRPVAERAQ DIGIWFHILA GLTHLAVISN AFLLAFSSDF LPRAYYRWTR
810 820 830 840 850
AHDLRGFLNF TLARAPSSFA AAHNRTCRYR AFRDDDGHYS QTYWNLLAIR
860 870 880 890 900
LAFVIVFEHV VFSVGRLLDL LVPDIPESVE IKVKREYYLA KQALAENEVL
910 920 930
FGTNGTKDEQ PEGSELSSHW TPFTVPKASQ LQQ
Length:933
Mass (Da):105,532
Last modified:May 31, 2011 - v2
Checksum:iD6FD42578A4BD773
GO
Isoform 2 (identifier: Q6IWH7-2) [UniParc]FASTAAdd to basket

Also known as: NGEP-S

The sequence of this isoform differs from the canonical sequence as follows:
     110-110: Missing.
     158-180: QDVQDGNTTVHYALLSASWAVLC → VRGGCHGQGPRPCIHSVTHDLAA
     181-933: Missing.

Show »
Length:179
Mass (Da):19,590
Checksum:iA300C8733A94485B
GO
Isoform 3 (identifier: Q6IWH7-3) [UniParc]FASTAAdd to basket

Also known as: D-TMPP

The sequence of this isoform differs from the canonical sequence as follows:
     110-110: Missing.
     859-859: H → VAEAPAGSPIHGMRPRPCALPNSSTW
     860-933: Missing.

Show »
Length:883
Mass (Da):99,754
Checksum:iA91DCD1079F7AB64
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti67 – 671V → I.
Corresponds to variant rs2302054 [ dbSNP | Ensembl ].
VAR_032616
Natural varianti912 – 9121E → K.1 Publication
Corresponds to variant rs7590653 [ dbSNP | Ensembl ].
VAR_065166

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei110 – 1101Missing in isoform 2 and isoform 3. 1 PublicationVSP_026004
Alternative sequencei158 – 18023QDVQD…WAVLC → VRGGCHGQGPRPCIHSVTHD LAA in isoform 2. 1 PublicationVSP_026005Add
BLAST
Alternative sequencei181 – 933753Missing in isoform 2. 1 PublicationVSP_026006Add
BLAST
Alternative sequencei859 – 8591H → VAEAPAGSPIHGMRPRPCAL PNSSTW in isoform 3. CuratedVSP_026007
Alternative sequencei860 – 93374Missing in isoform 3. CuratedVSP_026008Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY617079 mRNA. Translation: AAT40139.1.
AY617080 mRNA. Translation: AAT40140.1.
AC005237 Genomic DNA. No translation available.
AC104841 Genomic DNA. No translation available.
AC111201 Genomic DNA. No translation available.
CCDSiCCDS33423.1. [Q6IWH7-1]
CCDS46563.1. [Q6IWH7-2]
RefSeqiNP_001001666.1. NM_001001666.3. [Q6IWH7-2]
NP_001001891.2. NM_001001891.3. [Q6IWH7-1]
UniGeneiHs.163909.

Genome annotation databases

EnsembliENST00000274979; ENSP00000274979; ENSG00000146205. [Q6IWH7-1]
ENST00000402430; ENSP00000385418; ENSG00000146205. [Q6IWH7-3]
ENST00000402530; ENSP00000383985; ENSG00000146205. [Q6IWH7-2]
GeneIDi50636.
KEGGihsa:50636.
UCSCiuc002waw.3. human. [Q6IWH7-2]
uc002wax.2. human. [Q6IWH7-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY617079 mRNA. Translation: AAT40139.1.
AY617080 mRNA. Translation: AAT40140.1.
AC005237 Genomic DNA. No translation available.
AC104841 Genomic DNA. No translation available.
AC111201 Genomic DNA. No translation available.
CCDSiCCDS33423.1. [Q6IWH7-1]
CCDS46563.1. [Q6IWH7-2]
RefSeqiNP_001001666.1. NM_001001666.3. [Q6IWH7-2]
NP_001001891.2. NM_001001891.3. [Q6IWH7-1]
UniGeneiHs.163909.

3D structure databases

ProteinModelPortaliQ6IWH7.
SMRiQ6IWH7. Positions 332-792.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9606.ENSP00000274979.

Polymorphism and mutation databases

BioMutaiANO7.
DMDMi334302764.

Proteomic databases

PaxDbiQ6IWH7.
PRIDEiQ6IWH7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000274979; ENSP00000274979; ENSG00000146205. [Q6IWH7-1]
ENST00000402430; ENSP00000385418; ENSG00000146205. [Q6IWH7-3]
ENST00000402530; ENSP00000383985; ENSG00000146205. [Q6IWH7-2]
GeneIDi50636.
KEGGihsa:50636.
UCSCiuc002waw.3. human. [Q6IWH7-2]
uc002wax.2. human. [Q6IWH7-1]

Organism-specific databases

CTDi50636.
GeneCardsiGC02P242127.
HGNCiHGNC:31677. ANO7.
HPAiHPA035730.
HPA035731.
MIMi605096. gene.
neXtProtiNX_Q6IWH7.
PharmGKBiPA32980.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG320103.
GeneTreeiENSGT00760000119015.
HOGENOMiHOG000006509.
HOVERGENiHBG069519.
InParanoidiQ6IWH7.
OMAiILAKTPY.
OrthoDBiEOG7BS48W.
PhylomeDBiQ6IWH7.
TreeFamiTF314265.

Enzyme and pathway databases

ReactomeiREACT_160189. Stimuli-sensing channels.

Miscellaneous databases

GenomeRNAii50636.
NextBioi53174.
PROiQ6IWH7.
SOURCEiSearch...

Gene expression databases

BgeeiQ6IWH7.
CleanExiHS_ANO7.
ExpressionAtlasiQ6IWH7. baseline and differential.
GenevisibleiQ6IWH7. HS.

Family and domain databases

InterProiIPR007632. Anoctamin.
[Graphical view]
PANTHERiPTHR12308. PTHR12308. 1 hit.
PfamiPF04547. Anoctamin. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "NGEP, a gene encoding a membrane protein detected only in prostate cancer and normal prostate."
    Bera T.K., Das S., Maeda H., Beers R., Wolfgang C.D., Kumar V., Hahn Y., Lee B., Pastan I.
    Proc. Natl. Acad. Sci. U.S.A. 101:3059-3064(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), VARIANT LYS-912, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, TOPOLOGY.
  2. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
    Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
    , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
    Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "Prediction of gene function by genome-scale expression analysis: prostate cancer-associated genes."
    Walker M.G., Volkmuth W., Sprinzak E., Hodgson D., Klingler T.
    Genome Res. 9:1198-1203(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.
  4. "Characterization of human TMEM16G gene in silico."
    Katoh M., Katoh M.
    Int. J. Mol. Med. 14:759-764(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.
  5. "D-TMPP: a novel androgen-regulated gene preferentially expressed in prostate and prostate cancer that is the first characterized member of an eukaryotic gene family."
    Kiessling A., Weigle B., Fuessel S., Ebner R., Meye A., Rieger M.A., Schmitz M., Temme A., Bachmann M., Wirth M.P., Rieber E.P.
    Prostate 64:387-400(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: ALTERNATIVE SPLICING (ISOFORM 3), TISSUE SPECIFICITY, INDUCTION BY ANDROGEN.
  6. "NGEP, a prostate-specific plasma membrane protein that promotes the association of LNCaP cells."
    Das S., Hahn Y., Nagata S., Willingham M.C., Bera T.K., Lee B., Pastan I.
    Cancer Res. 67:1594-1601(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  7. "Topology of NGEP, a prostate-specific cell:cell junction protein widely expressed in many cancers of different grade level."
    Das S., Hahn Y., Walker D.A., Nagata S., Willingham M.C., Peehl D.M., Bera T.K., Lee B., Pastan I.
    Cancer Res. 68:6306-6312(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION AT ASN-809 AND ASN-824, MEMBRANE TOPOLOGY.
  8. Cited for: SUBCELLULAR LOCATION.
  9. "Physiological roles and diseases of Tmem16/Anoctamin proteins: are they all chloride channels?"
    Duran C., Hartzell H.C.
    Acta Pharmacol. Sin. 32:685-692(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  10. Cited for: REVIEW.
  11. "ANOs 3-7 in the anoctamin/Tmem16 Cl- channel family are intracellular proteins."
    Duran C., Qu Z., Osunkoya A.O., Cui Y., Hartzell H.C.
    Am. J. Physiol. 302:C482-C493(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: ABSENCE OF CALCIUM-ACTIVATED CHLORIDE CHANNEL ACTIVITY, SUBCELLULAR LOCATION.
  12. "The anoctamin (TMEM16) gene family: calcium-activated chloride channels come of age."
    Winpenny J.P., Gray M.A.
    Exp. Physiol. 97:175-176(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  13. "The anoctamin family: TMEM16A and TMEM16B as calcium-activated chloride channels."
    Scudieri P., Sondo E., Ferrera L., Galietta L.J.
    Exp. Physiol. 97:177-183(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW, ABSENCE OF CALCIUM-ACTIVATED CHLORIDE CHANNEL ACTIVITY.
  14. "Anoctamins are a family of Ca2+ activated Cl- channels."
    Tian Y., Schreiber R., Kunzelmann K.
    J. Cell Sci. 125:4991-4998(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiANO7_HUMAN
AccessioniPrimary (citable) accession number: Q6IWH7
Secondary accession number(s): Q6IWH6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: May 31, 2011
Last modified: June 24, 2015
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

The term 'anoctamin' was coined because these channels are anion selective and have eight (OCT) transmembrane segments. There is some dissatisfaction in the field with the Ano nomenclature because it is not certain that all the members of this family are anion channels or have the 8-transmembrane topology.

Caution

It is uncertain whether Met-1 or Met-55 is the initiator.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.