Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q6IWH7 (ANO7_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Anoctamin-7
Alternative name(s):
Dresden transmembrane protein of the prostate
Short name=D-TMPP
IPCA-5
New gene expressed in prostate
Prostate cancer-associated protein 5
Transmembrane protein 16G
Gene names
Name:ANO7
Synonyms:NGEP, PCANAP5, TMEM16G
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length933 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Does not exhibit calcium-activated chloride channel (CaCC) activity. May play a role in cell-cell interactions. Ref.6

Subcellular location

Isoform 1: Cell membrane; Multi-pass membrane protein. Cell junction. Endoplasmic reticulum. Note: Concentrates at sites of cell-cell contact. Shows an intracellular localization according to Ref.11 and Ref.8. Ref.1 Ref.6 Ref.8 Ref.11 Ref.14

Isoform 2: Cytoplasmcytosol Ref.1 Ref.6 Ref.8 Ref.11 Ref.14.

Tissue specificity

Specifically expressed in epithelial cells of the prostate (at protein level). Ref.1 Ref.5 Ref.6

Induction

Up-regulated by androgen. Ref.5

Miscellaneous

The term 'anoctamin' was coined because these channels are anion selective and have eight (OCT) transmembrane segments. There is some dissatisfaction in the field with the Ano nomenclature because it is not certain that all the members of this family are anion channels or have the 8-transmembrane topology.

Sequence similarities

Belongs to the anoctamin family.

Caution

It is uncertain whether Met-1 or Met-55 is the initiator.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q6IWH7-1)

Also known as: NGEP-L;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q6IWH7-2)

Also known as: NGEP-S;

The sequence of this isoform differs from the canonical sequence as follows:
     110-110: Missing.
     158-180: QDVQDGNTTVHYALLSASWAVLC → VRGGCHGQGPRPCIHSVTHDLAA
     181-933: Missing.
Isoform 3 (identifier: Q6IWH7-3)

Also known as: D-TMPP;

The sequence of this isoform differs from the canonical sequence as follows:
     110-110: Missing.
     859-859: H → VAEAPAGSPIHGMRPRPCALPNSSTW
     860-933: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 933933Anoctamin-7
PRO_0000289326

Regions

Topological domain1 – 355355Cytoplasmic Potential
Transmembrane356 – 37621Helical; Potential
Topological domain377 – 42044Extracellular Potential
Transmembrane421 – 44121Helical; Potential
Topological domain442 – 49958Cytoplasmic Potential
Transmembrane500 – 52021Helical; Potential
Topological domain521 – 55030Extracellular Potential
Transmembrane551 – 57121Helical; Potential
Topological domain572 – 58817Cytoplasmic Potential
Transmembrane589 – 60921Helical; Potential
Topological domain610 – 714105Extracellular Potential
Transmembrane715 – 73521Helical; Potential
Topological domain736 – 76328Cytoplasmic Potential
Transmembrane764 – 78421Helical; Potential
Topological domain785 – 84359Extracellular Potential
Transmembrane844 – 86421Helical; Potential
Topological domain865 – 93369Cytoplasmic Potential

Amino acid modifications

Glycosylation8091N-linked (GlcNAc...) Ref.7
Glycosylation8241N-linked (GlcNAc...) Ref.7

Natural variations

Alternative sequence1101Missing in isoform 2 and isoform 3.
VSP_026004
Alternative sequence158 – 18023QDVQD…WAVLC → VRGGCHGQGPRPCIHSVTHD LAA in isoform 2.
VSP_026005
Alternative sequence181 – 933753Missing in isoform 2.
VSP_026006
Alternative sequence8591H → VAEAPAGSPIHGMRPRPCAL PNSSTW in isoform 3.
VSP_026007
Alternative sequence860 – 93374Missing in isoform 3.
VSP_026008
Natural variant671V → I.
Corresponds to variant rs2302054 [ dbSNP | Ensembl ].
VAR_032616
Natural variant9121E → K. Ref.1
Corresponds to variant rs7590653 [ dbSNP | Ensembl ].
VAR_065166

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (NGEP-L) [UniParc].

Last modified May 31, 2011. Version 2.
Checksum: D6FD42578A4BD773

FASTA933105,532
        10         20         30         40         50         60 
MRMAATAWAG LQGPPLPTLC PAVRTGLYCR DQAHAERWAM TSETSSGSHC ARSRMLRRRA 

        70         80         90        100        110        120 
QEEDSTVLID VSPPEAEKRG SYGSTAHASE PGGQQAAACR AGSPAKPRIA DFVLVWEEDL 

       130        140        150        160        170        180 
KLDRQQDSAA RDRTDMHRTW RETFLDNLRA AGLCVDQQDV QDGNTTVHYA LLSASWAVLC 

       190        200        210        220        230        240 
YYAEDLRLKL PLQELPNQAS NWSAGLLAWL GIPNVLLEVV PDVPPEYYSC RFRVNKLPRF 

       250        260        270        280        290        300 
LGSDNQDTFF TSTKRHQILF EILAKTPYGH EKKNLLGIHQ LLAEGVLSAA FPLHDGPFKT 

       310        320        330        340        350        360 
PPEGPQAPRL NQRQVLFQHW ARWGKWNKYQ PLDHVRRYFG EKVALYFAWL GFYTGWLLPA 

       370        380        390        400        410        420 
AVVGTLVFLV GCFLVFSDIP TQELCGSKDS FEMCPLCLDC PFWLLSSACA LAQAGRLFDH 

       430        440        450        460        470        480 
GGTVFFSLFM ALWAVLLLEY WKRKSATLAY RWDCSDYEDT EERPRPQFAA SAPMTAPNPI 

       490        500        510        520        530        540 
TGEDEPYFPE RSRARRMLAG SVVIVVMVAV VVMCLVSIIL YRAIMAIVVS RSGNTLLAAW 

       550        560        570        580        590        600 
ASRIASLTGS VVNLVFILIL SKIYVSLAHV LTRWEMHRTQ TKFEDAFTLK VFIFQFVNFY 

       610        620        630        640        650        660 
SSPVYIAFFK GRFVGYPGNY HTLFGVRNEE CAAGGCLIEL AQELLVIMVG KQVINNMQEV 

       670        680        690        700        710        720 
LIPKLKGWWQ KFRLRSKKRK AGASAGASQG PWEDDYELVP CEGLFDEYLE MVLQFGFVTI 

       730        740        750        760        770        780 
FVAACPLAPL FALLNNWVEI RLDARKFVCE YRRPVAERAQ DIGIWFHILA GLTHLAVISN 

       790        800        810        820        830        840 
AFLLAFSSDF LPRAYYRWTR AHDLRGFLNF TLARAPSSFA AAHNRTCRYR AFRDDDGHYS 

       850        860        870        880        890        900 
QTYWNLLAIR LAFVIVFEHV VFSVGRLLDL LVPDIPESVE IKVKREYYLA KQALAENEVL 

       910        920        930 
FGTNGTKDEQ PEGSELSSHW TPFTVPKASQ LQQ 

« Hide

Isoform 2 (NGEP-S) [UniParc].

Checksum: A300C8733A94485B
Show »

FASTA17919,590
Isoform 3 (D-TMPP) [UniParc].

Checksum: A91DCD1079F7AB64
Show »

FASTA88399,754

References

« Hide 'large scale' references
[1]"NGEP, a gene encoding a membrane protein detected only in prostate cancer and normal prostate."
Bera T.K., Das S., Maeda H., Beers R., Wolfgang C.D., Kumar V., Hahn Y., Lee B., Pastan I.
Proc. Natl. Acad. Sci. U.S.A. 101:3059-3064(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), VARIANT LYS-912, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, TOPOLOGY.
[2]"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. expand/collapse author list , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"Prediction of gene function by genome-scale expression analysis: prostate cancer-associated genes."
Walker M.G., Volkmuth W., Sprinzak E., Hodgson D., Klingler T.
Genome Res. 9:1198-1203(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION.
[4]"Characterization of human TMEM16G gene in silico."
Katoh M., Katoh M.
Int. J. Mol. Med. 14:759-764(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION.
[5]"D-TMPP: a novel androgen-regulated gene preferentially expressed in prostate and prostate cancer that is the first characterized member of an eukaryotic gene family."
Kiessling A., Weigle B., Fuessel S., Ebner R., Meye A., Rieger M.A., Schmitz M., Temme A., Bachmann M., Wirth M.P., Rieber E.P.
Prostate 64:387-400(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: ALTERNATIVE SPLICING (ISOFORM 3), TISSUE SPECIFICITY, INDUCTION BY ANDROGEN.
[6]"NGEP, a prostate-specific plasma membrane protein that promotes the association of LNCaP cells."
Das S., Hahn Y., Nagata S., Willingham M.C., Bera T.K., Lee B., Pastan I.
Cancer Res. 67:1594-1601(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[7]"Topology of NGEP, a prostate-specific cell:cell junction protein widely expressed in many cancers of different grade level."
Das S., Hahn Y., Walker D.A., Nagata S., Willingham M.C., Peehl D.M., Bera T.K., Lee B., Pastan I.
Cancer Res. 68:6306-6312(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION AT ASN-809 AND ASN-824, MEMBRANE TOPOLOGY.
[8]"Expression and function of epithelial anoctamins."
Schreiber R., Uliyakina I., Kongsuphol P., Warth R., Mirza M., Martins J.R., Kunzelmann K.
J. Biol. Chem. 285:7838-7845(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[9]"Physiological roles and diseases of Tmem16/Anoctamin proteins: are they all chloride channels?"
Duran C., Hartzell H.C.
Acta Pharmacol. Sin. 32:685-692(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
[10]"Anoctamins."
Kunzelmann K., Tian Y., Martins J.R., Faria D., Kongsuphol P., Ousingsawat J., Thevenod F., Roussa E., Rock J., Schreiber R.
Pflugers Arch. 462:195-208(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
[11]"ANOs 3-7 in the anoctamin/Tmem16 Cl- channel family are intracellular proteins."
Duran C., Qu Z., Osunkoya A.O., Cui Y., Hartzell H.C.
Am. J. Physiol. 302:C482-C493(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ABSENCE OF CALCIUM-ACTIVATED CHLORIDE CHANNEL ACTIVITY, SUBCELLULAR LOCATION.
[12]"The anoctamin (TMEM16) gene family: calcium-activated chloride channels come of age."
Winpenny J.P., Gray M.A.
Exp. Physiol. 97:175-176(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
[13]"The anoctamin family: TMEM16A and TMEM16B as calcium-activated chloride channels."
Scudieri P., Sondo E., Ferrera L., Galietta L.J.
Exp. Physiol. 97:177-183(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW, ABSENCE OF CALCIUM-ACTIVATED CHLORIDE CHANNEL ACTIVITY.
[14]"Anoctamins are a family of Ca2+ activated Cl- channels."
Tian Y., Schreiber R., Kunzelmann K.
J. Cell Sci. 125:4991-4998(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY617079 mRNA. Translation: AAT40139.1.
AY617080 mRNA. Translation: AAT40140.1.
AC005237 Genomic DNA. No translation available.
AC104841 Genomic DNA. No translation available.
AC111201 Genomic DNA. No translation available.
RefSeqNP_001001666.1. NM_001001666.3.
NP_001001891.2. NM_001001891.3.
UniGeneHs.163909.

3D structure databases

ProteinModelPortalQ6IWH7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000274979.

Polymorphism databases

DMDM334302764.

Proteomic databases

PaxDbQ6IWH7.
PRIDEQ6IWH7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000274979; ENSP00000274979; ENSG00000146205. [Q6IWH7-1]
ENST00000402430; ENSP00000385418; ENSG00000146205. [Q6IWH7-3]
ENST00000402530; ENSP00000383985; ENSG00000146205. [Q6IWH7-2]
GeneID50636.
KEGGhsa:50636.
UCSCuc002waw.3. human. [Q6IWH7-2]
uc002wax.2. human. [Q6IWH7-1]

Organism-specific databases

CTD50636.
GeneCardsGC02P242127.
HGNCHGNC:31677. ANO7.
HPAHPA035730.
HPA035731.
MIM605096. gene.
neXtProtNX_Q6IWH7.
PharmGKBPA32980.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG320103.
HOGENOMHOG000006509.
HOVERGENHBG069519.
InParanoidQ6IWH7.
OMAILAKTPY.
OrthoDBEOG7BS48W.
TreeFamTF314265.

Enzyme and pathway databases

ReactomeREACT_15518. Transmembrane transport of small molecules.

Gene expression databases

ArrayExpressQ6IWH7.
BgeeQ6IWH7.
CleanExHS_ANO7.
GenevestigatorQ6IWH7.

Family and domain databases

InterProIPR007632. Anoctamin.
[Graphical view]
PANTHERPTHR12308. PTHR12308. 1 hit.
PfamPF04547. Anoctamin. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi50636.
NextBio53174.
PROQ6IWH7.
SOURCESearch...

Entry information

Entry nameANO7_HUMAN
AccessionPrimary (citable) accession number: Q6IWH7
Secondary accession number(s): Q6IWH6
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: May 31, 2011
Last modified: April 16, 2014
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 2

Human chromosome 2: entries, gene names and cross-references to MIM