Q6IWH7 (ANO7_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Anoctamin-7

Alternative name(s):
Dresden transmembrane protein of the prostate
Short name=D-TMPP
IPCA-5
New gene expressed in prostate
Prostate cancer-associated protein 5
Transmembrane protein 16G

Gene names

Name:	ANO7
Synonyms:	NGEP, PCANAP5, TMEM16G

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	933 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Does not exhibit calcium-activated chloride channel (CaCC) activity. May play a role in cell-cell interactions. Ref.6
Subcellular location	Isoform 1: Cell membrane; Multi-pass membrane protein. Cell junction. Endoplasmic reticulum. Note: Concentrates at sites of cell-cell contact. Shows an intracellular localization according to Ref.11 and Ref.8. Ref.1 Ref.6 Ref.8 Ref.11 Ref.14 Isoform 2: Cytoplasm › cytosol Ref.1 Ref.6 Ref.8 Ref.11 Ref.14.
Tissue specificity	Specifically expressed in epithelial cells of the prostate (at protein level). Ref.1 Ref.5 Ref.6
Induction	Up-regulated by androgen. Ref.5
Miscellaneous	The term 'anoctamin' was coined because these channels are anion selective and have eight (OCT) transmembrane segments. There is some dissatisfaction in the field with the Ano nomenclature because it is not certain that all the members of this family are anion channels or have the 8-transmembrane topology.
Sequence similarities	Belongs to the anoctamin family.
Caution	It is uncertain whether Met-1 or Met-55 is the initiator.

Ontologies

Keywords
Cellular component	Cell junction Cell membrane Cytoplasm Endoplasmic reticulum Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Transmembrane Transmembrane helix
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	chloride transport Inferred from electronic annotation. Source: Compara
Cellular_component	cell junction Inferred from electronic annotation. Source: UniProtKB-SubCell cytosol Inferred from electronic annotation. Source: UniProtKB-SubCell endoplasmic reticulum Inferred from direct assay Ref.11. Source: UniProtKB integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW nucleus Inferred from electronic annotation. Source: Compara plasma membrane Inferred from direct assay Ref.1. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q6IWH7-1)

Also known as: NGEP-L;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q6IWH7-2)

Also known as: NGEP-S;

The sequence of this isoform differs from the canonical sequence as follows:
     110-110: Missing.
     158-180: QDVQDGNTTVHYALLSASWAVLC → VRGGCHGQGPRPCIHSVTHDLAA
     181-933: Missing.

Isoform 3 (identifier: Q6IWH7-3)

Also known as: D-TMPP;

The sequence of this isoform differs from the canonical sequence as follows:
     110-110: Missing.
     859-859: H → VAEAPAGSPIHGMRPRPCALPNSSTW
     860-933: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 933	933	Anoctamin-7		PRO_0000289326
Regions
	Topological domain	1 – 355	355	Cytoplasmic Potential
	Transmembrane	356 – 376	21	Helical; Potential
	Topological domain	377 – 420	44	Extracellular Potential
	Transmembrane	421 – 441	21	Helical; Potential
	Topological domain	442 – 499	58	Cytoplasmic Potential
	Transmembrane	500 – 520	21	Helical; Potential
	Topological domain	521 – 550	30	Extracellular Potential
	Transmembrane	551 – 571	21	Helical; Potential
	Topological domain	572 – 588	17	Cytoplasmic Potential
	Transmembrane	589 – 609	21	Helical; Potential
	Topological domain	610 – 714	105	Extracellular Potential
	Transmembrane	715 – 735	21	Helical; Potential
	Topological domain	736 – 763	28	Cytoplasmic Potential
	Transmembrane	764 – 784	21	Helical; Potential
	Topological domain	785 – 843	59	Extracellular Potential
	Transmembrane	844 – 864	21	Helical; Potential
	Topological domain	865 – 933	69	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	809	1	N-linked (GlcNAc...) Ref.7
	Glycosylation	824	1	N-linked (GlcNAc...) Ref.7
Natural variations
	Alternative sequence	110	1	Missing in isoform 2 and isoform 3.		VSP_026004
	Alternative sequence	158 – 180	23	QDVQD…WAVLC → VRGGCHGQGPRPCIHSVTHD LAA in isoform 2.		VSP_026005
	Alternative sequence	181 – 933	753	Missing in isoform 2.		VSP_026006
	Alternative sequence	859	1	H → VAEAPAGSPIHGMRPRPCAL PNSSTW in isoform 3.		VSP_026007
	Alternative sequence	860 – 933	74	Missing in isoform 3.		VSP_026008
	Natural variant	67	1	V → I. Corresponds to variant rs2302054 [ dbSNP | Ensembl ].		VAR_032616
	Natural variant	912	1	E → K. Ref.1 Corresponds to variant rs7590653 [ dbSNP | Ensembl ].		VAR_065166

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (NGEP-L) [UniParc]. Last modified May 31, 2011. Version 2. Checksum: D6FD42578A4BD773	FASTA	933	105,532
	10 20 30 40 50 60 MRMAATAWAG LQGPPLPTLC PAVRTGLYCR DQAHAERWAM TSETSSGSHC ARSRMLRRRA 70 80 90 100 110 120 QEEDSTVLID VSPPEAEKRG SYGSTAHASE PGGQQAAACR AGSPAKPRIA DFVLVWEEDL 130 140 150 160 170 180 KLDRQQDSAA RDRTDMHRTW RETFLDNLRA AGLCVDQQDV QDGNTTVHYA LLSASWAVLC 190 200 210 220 230 240 YYAEDLRLKL PLQELPNQAS NWSAGLLAWL GIPNVLLEVV PDVPPEYYSC RFRVNKLPRF 250 260 270 280 290 300 LGSDNQDTFF TSTKRHQILF EILAKTPYGH EKKNLLGIHQ LLAEGVLSAA FPLHDGPFKT 310 320 330 340 350 360 PPEGPQAPRL NQRQVLFQHW ARWGKWNKYQ PLDHVRRYFG EKVALYFAWL GFYTGWLLPA 370 380 390 400 410 420 AVVGTLVFLV GCFLVFSDIP TQELCGSKDS FEMCPLCLDC PFWLLSSACA LAQAGRLFDH 430 440 450 460 470 480 GGTVFFSLFM ALWAVLLLEY WKRKSATLAY RWDCSDYEDT EERPRPQFAA SAPMTAPNPI 490 500 510 520 530 540 TGEDEPYFPE RSRARRMLAG SVVIVVMVAV VVMCLVSIIL YRAIMAIVVS RSGNTLLAAW 550 560 570 580 590 600 ASRIASLTGS VVNLVFILIL SKIYVSLAHV LTRWEMHRTQ TKFEDAFTLK VFIFQFVNFY 610 620 630 640 650 660 SSPVYIAFFK GRFVGYPGNY HTLFGVRNEE CAAGGCLIEL AQELLVIMVG KQVINNMQEV 670 680 690 700 710 720 LIPKLKGWWQ KFRLRSKKRK AGASAGASQG PWEDDYELVP CEGLFDEYLE MVLQFGFVTI 730 740 750 760 770 780 FVAACPLAPL FALLNNWVEI RLDARKFVCE YRRPVAERAQ DIGIWFHILA GLTHLAVISN 790 800 810 820 830 840 AFLLAFSSDF LPRAYYRWTR AHDLRGFLNF TLARAPSSFA AAHNRTCRYR AFRDDDGHYS 850 860 870 880 890 900 QTYWNLLAIR LAFVIVFEHV VFSVGRLLDL LVPDIPESVE IKVKREYYLA KQALAENEVL 910 920 930 FGTNGTKDEQ PEGSELSSHW TPFTVPKASQ LQQ
	Isoform 2 (NGEP-S) [UniParc]. Checksum: A300C8733A94485B	FASTA	179	19,590
	10 20 30 40 50 60 MRMAATAWAG LQGPPLPTLC PAVRTGLYCR DQAHAERWAM TSETSSGSHC ARSRMLRRRA 70 80 90 100 110 120 QEEDSTVLID VSPPEAEKRG SYGSTAHASE PGGQQAAACR AGSPAKPRID FVLVWEEDLK 130 140 150 160 170 LDRQQDSAAR DRTDMHRTWR ETFLDNLRAA GLCVDQVRGG CHGQGPRPCI HSVTHDLAA
	Isoform 3 (D-TMPP) [UniParc]. Checksum: A91DCD1079F7AB64	FASTA	883	99,754
	10 20 30 40 50 60 MRMAATAWAG LQGPPLPTLC PAVRTGLYCR DQAHAERWAM TSETSSGSHC ARSRMLRRRA 70 80 90 100 110 120 QEEDSTVLID VSPPEAEKRG SYGSTAHASE PGGQQAAACR AGSPAKPRID FVLVWEEDLK 130 140 150 160 170 180 LDRQQDSAAR DRTDMHRTWR ETFLDNLRAA GLCVDQQDVQ DGNTTVHYAL LSASWAVLCY 190 200 210 220 230 240 YAEDLRLKLP LQELPNQASN WSAGLLAWLG IPNVLLEVVP DVPPEYYSCR FRVNKLPRFL 250 260 270 280 290 300 GSDNQDTFFT STKRHQILFE ILAKTPYGHE KKNLLGIHQL LAEGVLSAAF PLHDGPFKTP 310 320 330 340 350 360 PEGPQAPRLN QRQVLFQHWA RWGKWNKYQP LDHVRRYFGE KVALYFAWLG FYTGWLLPAA 370 380 390 400 410 420 VVGTLVFLVG CFLVFSDIPT QELCGSKDSF EMCPLCLDCP FWLLSSACAL AQAGRLFDHG 430 440 450 460 470 480 GTVFFSLFMA LWAVLLLEYW KRKSATLAYR WDCSDYEDTE ERPRPQFAAS APMTAPNPIT 490 500 510 520 530 540 GEDEPYFPER SRARRMLAGS VVIVVMVAVV VMCLVSIILY RAIMAIVVSR SGNTLLAAWA 550 560 570 580 590 600 SRIASLTGSV VNLVFILILS KIYVSLAHVL TRWEMHRTQT KFEDAFTLKV FIFQFVNFYS 610 620 630 640 650 660 SPVYIAFFKG RFVGYPGNYH TLFGVRNEEC AAGGCLIELA QELLVIMVGK QVINNMQEVL 670 680 690 700 710 720 IPKLKGWWQK FRLRSKKRKA GASAGASQGP WEDDYELVPC EGLFDEYLEM VLQFGFVTIF 730 740 750 760 770 780 VAACPLAPLF ALLNNWVEIR LDARKFVCEY RRPVAERAQD IGIWFHILAG LTHLAVISNA 790 800 810 820 830 840 FLLAFSSDFL PRAYYRWTRA HDLRGFLNFT LARAPSSFAA AHNRTCRYRA FRDDDGHYSQ 850 860 870 880 TYWNLLAIRL AFVIVFEVAE APAGSPIHGM RPRPCALPNS STW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"NGEP, a gene encoding a membrane protein detected only in prostate cancer and normal prostate." Bera T.K., Das S., Maeda H., Beers R., Wolfgang C.D., Kumar V., Hahn Y., Lee B., Pastan I. Proc. Natl. Acad. Sci. U.S.A. 101:3059-3064(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), VARIANT LYS-912, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, TOPOLOGY.
[2]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Prediction of gene function by genome-scale expression analysis: prostate cancer-associated genes." Walker M.G., Volkmuth W., Sprinzak E., Hodgson D., Klingler T. Genome Res. 9:1198-1203(1999) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION.
[4]	"Characterization of human TMEM16G gene in silico." Katoh M., Katoh M. Int. J. Mol. Med. 14:759-764(2004) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION.
[5]	"D-TMPP: a novel androgen-regulated gene preferentially expressed in prostate and prostate cancer that is the first characterized member of an eukaryotic gene family." Kiessling A., Weigle B., Fuessel S., Ebner R., Meye A., Rieger M.A., Schmitz M., Temme A., Bachmann M., Wirth M.P., Rieber E.P. Prostate 64:387-400(2005) [PubMed] [Europe PMC] [Abstract] Cited for: ALTERNATIVE SPLICING (ISOFORM 3), TISSUE SPECIFICITY, INDUCTION BY ANDROGEN.
[6]	"NGEP, a prostate-specific plasma membrane protein that promotes the association of LNCaP cells." Das S., Hahn Y., Nagata S., Willingham M.C., Bera T.K., Lee B., Pastan I. Cancer Res. 67:1594-1601(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[7]	"Topology of NGEP, a prostate-specific cell:cell junction protein widely expressed in many cancers of different grade level." Das S., Hahn Y., Walker D.A., Nagata S., Willingham M.C., Peehl D.M., Bera T.K., Lee B., Pastan I. Cancer Res. 68:6306-6312(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION AT ASN-809 AND ASN-824, MEMBRANE TOPOLOGY.
[8]	"Expression and function of epithelial anoctamins." Schreiber R., Uliyakina I., Kongsuphol P., Warth R., Mirza M., Martins J.R., Kunzelmann K. J. Biol. Chem. 285:7838-7845(2010) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[9]	"Physiological roles and diseases of Tmem16/Anoctamin proteins: are they all chloride channels?" Duran C., Hartzell H.C. Acta Pharmacol. Sin. 32:685-692(2011) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.
[10]	"Anoctamins." Kunzelmann K., Tian Y., Martins J.R., Faria D., Kongsuphol P., Ousingsawat J., Thevenod F., Roussa E., Rock J., Schreiber R. Pflugers Arch. 462:195-208(2011) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.
[11]	"ANOs 3-7 in the anoctamin/Tmem16 Cl- channel family are intracellular proteins." Duran C., Qu Z., Osunkoya A.O., Cui Y., Hartzell H.C. Am. J. Physiol. 302:C482-C493(2012) [PubMed] [Europe PMC] [Abstract] Cited for: ABSENCE OF CALCIUM-ACTIVATED CHLORIDE CHANNEL ACTIVITY, SUBCELLULAR LOCATION.
[12]	"The anoctamin (TMEM16) gene family: calcium-activated chloride channels come of age." Winpenny J.P., Gray M.A. Exp. Physiol. 97:175-176(2012) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.
[13]	"The anoctamin family: TMEM16A and TMEM16B as calcium-activated chloride channels." Scudieri P., Sondo E., Ferrera L., Galietta L.J. Exp. Physiol. 97:177-183(2012) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW, ABSENCE OF CALCIUM-ACTIVATED CHLORIDE CHANNEL ACTIVITY.
[14]	"Anoctamins are a family of Ca2+ activated Cl- channels." Tian Y., Schreiber R., Kunzelmann K. J. Cell Sci. 125:4991-4998(2012) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY617079 mRNA. Translation: AAT40139.1. AY617080 mRNA. Translation: AAT40140.1. AC005237 Genomic DNA. No translation available. AC104841 Genomic DNA. No translation available. AC111201 Genomic DNA. No translation available.
IPI	IPI00170999. IPI00420050. IPI00848160.
RefSeq	NP_001001666.1. NM_001001666.3. NP_001001891.2. NM_001001891.3.
UniGene	Hs.163909.
3D structure databases
ProteinModelPortal	Q6IWH7.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000274979.
Polymorphism databases
DMDM	74748852.
Proteomic databases
PaxDb	Q6IWH7.
PRIDE	Q6IWH7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000274979; ENSP00000274979; ENSG00000146205. ENST00000402430; ENSP00000385418; ENSG00000146205. ENST00000402530; ENSP00000383985; ENSG00000146205.
GeneID	50636.
KEGG	hsa:50636.
UCSC	uc002waw.3. human. uc002wax.2. human.
Organism-specific databases
CTD	50636.
GeneCards	GC02P242127.
HGNC	HGNC:31677. ANO7.
HPA	HPA035730.
MIM	605096. gene.
neXtProt	NX_Q6IWH7.
PharmGKB	PA32980.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG320103.
HOGENOM	HOG000006509.
HOVERGEN	HBG069519.
InParanoid	Q6IWH7.
OMA	IKVKREY.
OrthoDB	EOG40GCQD.
Gene expression databases
ArrayExpress	Q6IWH7.
Bgee	Q6IWH7.
CleanEx	HS_ANO7.
Genevestigator	Q6IWH7.
Family and domain databases
InterPro	IPR007632. Anoctamin. [Graphical view]
PANTHER	PTHR12308. PTHR12308. 1 hit.
Pfam	PF04547. Anoctamin. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	50636.
NextBio	53174.
SOURCE	Search...

Entry information

Entry name

ANO7_HUMAN

Accession

Primary (citable) accession number: Q6IWH7
Secondary accession number(s): Q6IWH6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	May 31, 2011
Last modified:	April 3, 2013
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.