Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q6IVY4

- SSH_XENLA

UniProt

Q6IVY4 - SSH_XENLA

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Protein phosphatase Slingshot homolog

Gene

ssh

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Protein phosphatase which regulates actin filament dynamics. Dephosphorylates and activates the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly. Required for completion of the gastrulation movement and for cytokinesis.2 Publications

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation
[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei380 – 3801Phosphocysteine intermediateCurated

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. protein tyrosine/serine/threonine phosphatase activity Source: InterPro
  3. protein tyrosine phosphatase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Actin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase Slingshot homolog (EC:3.1.3.16, EC:3.1.3.48)
Short name:
xSSH
Alternative name(s):
Slingshot-related protein
Gene namesi
Name:ssh
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-5816852. ssh3.

Subcellular locationi

Cytoplasmcytoskeleton 1 Publication. Cleavage furrow 1 Publication. Midbody 1 Publication
Note: Also localizes to the cleavage furrow and the midbody during cytokinesis.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. cytoskeleton Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi380 – 3801C → S: Abrogates phosphatase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 691691Protein phosphatase Slingshot homologPRO_0000094848Add
BLAST

Expressioni

Developmental stagei

Maternally expressed in the early blastomere. Expressed in the involuting mesodermal cells and ectodermal cells of early gastrula stage embryos. Expression increases from stage 10.5, when the gastrulation movement occurs. Expression is concentrated at blastopore lips and the dorsal site of stage 11 embryos. Present in head structures and the trunk at the neurula and tailbud stages.1 Publication

Interactioni

Subunit structurei

Interacts with actin and this stimulates phosphatase activity.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ6IVY4.
SMRiQ6IVY4. Positions 296-436.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini295 – 435141Tyrosine-protein phosphataseAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili532 – 58049Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Coiled coil

Phylogenomic databases

KOiK05766.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
3.90.190.10. 1 hit.
InterProiIPR014876. DEK_C.
IPR000340. Dual-sp_phosphatase_cat-dom.
IPR020422. Dual-sp_phosphatase_subgr_cat.
IPR024950. DUSP.
IPR009057. Homeodomain-like.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
[Graphical view]
PANTHERiPTHR10159. PTHR10159. 1 hit.
PfamiPF08766. DEK_C. 1 hit.
PF00782. DSPc. 1 hit.
[Graphical view]
SMARTiSM00195. DSPc. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q6IVY4-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALVTLQVSS LDVGSNITPV QDDEKSRRKR MQRRQSFVMV KGAALLLQDE
60 70 80 90 100
GEPIDTREPL SSSGPNEQQI HLQSMLRLLR EEDTLTLAVR LEPVRSCLTR
110 120 130 140 150
YLLVVSSTGK SNVEETLLLG VDFPHDGSLC CTIGTVLPIW SNTQVFLDGD
160 170 180 190 200
GGFTVTSGMD IRTFKPISVQ TMWSLLQMLH KACESALSNN VISSSLYSGL
210 220 230 240 250
IYYQSNRSSP QVCLNAWTAS PDIESARRDP ATPEREETER IIKLKLRDIL
260 270 280 290 300
RESDLENITS KEVRSALEQH TLCALQDYKE FIDNEMIIIL AQMDRPSEIF
310 320 330 340 350
PYLYLGSEWN ASNLEELQKN KVSHILNVTR EIDNFFPELF MYLNIRVLDE
360 370 380 390 400
ENTNLMQYWK ETHAFITTAR HQGSRVLVHC KMGVSRSAST VIAYAMKEYE
410 420 430 440 450
WTLETAIRHV KERRSIVQPN AGFMRQLQTY QGILGASKQR HSYLWDPSSA
460 470 480 490 500
PSLPLMSPPP KNFSSPTTSP LTPRLQKMNL RTLMRSISEM EAADTISEEK
510 520 530 540 550
ESTVVLEETT LKQNFNVLES SSNNLQVTPK RNEHVLSKEQ IIQEEKKVME
560 570 580 590 600
LEKGPEWVVK NNVLEEMKET EERELPNFEL PMSLNQSRER DQETIKESSV
610 620 630 640 650
ITQGSSSLDE VFESSTPTRS PEMASYACQK IQYFEQHSEG YSKVCFVDNL
660 670 680 690
QSVSEEEKVT LVSKQLQTDE HGERKARITR QQNVIDTHEE L
Length:691
Mass (Da):78,904
Last modified:November 22, 2005 - v2
Checksum:iC0EB36F3830AC20E
GO
Isoform 2 (identifier: Q6IVY4-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-38: Missing.

Show »
Length:653
Mass (Da):74,545
Checksum:iF20DDABDF36494A2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti65 – 651P → R in AAT39429. (PubMed:16219976)Curated
Sequence conflicti624 – 6241A → V in AAH80117. 1 PublicationCurated
Sequence conflicti627 – 6271A → K in AAH80117. 1 PublicationCurated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 3838Missing in isoform 2. 1 PublicationVSP_016338Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY233266 mRNA. Translation: AAP57715.1.
AY618876 mRNA. Translation: AAT39429.1.
BC080117 mRNA. Translation: AAH80117.2.
BC094472 mRNA. Translation: AAH94472.1.
RefSeqiNP_001082641.1. NM_001089172.1.
UniGeneiXl.2010.

Genome annotation databases

GeneIDi398618.
KEGGixla:398618.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY233266 mRNA. Translation: AAP57715.1 .
AY618876 mRNA. Translation: AAT39429.1 .
BC080117 mRNA. Translation: AAH80117.2 .
BC094472 mRNA. Translation: AAH94472.1 .
RefSeqi NP_001082641.1. NM_001089172.1.
UniGenei Xl.2010.

3D structure databases

ProteinModelPortali Q6IVY4.
SMRi Q6IVY4. Positions 296-436.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 398618.
KEGGi xla:398618.

Organism-specific databases

CTDi 54961.
Xenbasei XB-GENE-5816852. ssh3.

Phylogenomic databases

KOi K05766.

Family and domain databases

Gene3Di 1.10.10.60. 1 hit.
3.90.190.10. 1 hit.
InterProi IPR014876. DEK_C.
IPR000340. Dual-sp_phosphatase_cat-dom.
IPR020422. Dual-sp_phosphatase_subgr_cat.
IPR024950. DUSP.
IPR009057. Homeodomain-like.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
[Graphical view ]
PANTHERi PTHR10159. PTHR10159. 1 hit.
Pfami PF08766. DEK_C. 1 hit.
PF00782. DSPc. 1 hit.
[Graphical view ]
SMARTi SM00195. DSPc. 1 hit.
[Graphical view ]
SUPFAMi SSF52799. SSF52799. 1 hit.
PROSITEi PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Functional involvement of Xenopus homologue of ADF/cofilin phosphatase, Slingshot (XSSH), in the gastrulation movement."
    Tanaka K., Nishio R., Haneda K., Abe H.
    Zool. Sci. 22:955-969(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, DEVELOPMENTAL STAGE, MUTAGENESIS OF CYS-380.
  2. NIH - Xenopus Gene Collection (XGC) project
    Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Eye and Oocyte.
  3. "Involvement of slingshot in the Rho-mediated dephosphorylation of ADF/cofilin during Xenopus cleavage."
    Tanaka K., Okubo Y., Abe H.
    Zool. Sci. 22:971-984(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH ACTIN, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiSSH_XENLA
AccessioniPrimary (citable) accession number: Q6IVY4
Secondary accession number(s): Q505N4, Q68ET3, Q7T2T3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: November 22, 2005
Last modified: October 29, 2014
This is version 64 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Tyrosine phosphatase activity has not been demonstrated for this protein to date.

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3