Q6IVY4 (SSH_XENLA) Reviewed, UniProtKB/Swiss-Prot
Last modified
March 6, 2013.
Version 58.
History...
Clusters with 
Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Protein phosphatase Slingshot homolog Short name=xSSH EC=3.1.3.16 EC=3.1.3.48 Alternative name(s): Slingshot-related protein | ||
| Gene names |
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| Organism | Xenopus laevis (African clawed frog) | ||
| Taxonomic identifier | 8355 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus |
Protein attributes
| Sequence length | 691 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Protein phosphatase which regulates actin filament dynamics. Dephosphorylates and activates the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly. Required for completion of the gastrulation movement and for cytokinesis. Ref.1 Ref.3 |
| Catalytic activity | Protein tyrosine phosphate + H2O = protein tyrosine + phosphate. A phosphoprotein + H2O = a protein + phosphate. |
| Subunit structure | Interacts with actin and this stimulates phosphatase activity. Ref.3 |
| Subcellular location | Cytoplasm › cytoskeleton. Cleavage furrow. Midbody. Note: Also localizes to the cleavage furrow and the midbody during cytokinesis. Ref.3 |
| Developmental stage | Maternally expressed in the early blastomere. Expressed in the involuting mesodermal cells and ectodermal cells of early gastrula stage embryos. Expression increases from stage 10.5, when the gastrulation movement occurs. Expression is concentrated at blastopore lips and the dorsal site of stage 11 embryos. Present in head structures and the trunk at the neurula and tailbud stages. Ref.1 |
| Miscellaneous | Tyrosine phosphatase activity has not been demonstrated for this protein to date. |
| Sequence similarities | Belongs to the protein-tyrosine phosphatase family. Contains 1 tyrosine-protein phosphatase domain. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Cytoplasm Cytoskeleton |
| Coding sequence diversity | Alternative splicing |
| Domain | Coiled coil |
| Ligand | Actin-binding |
| Molecular function | Hydrolase Protein phosphatase |
| Gene Ontology (GO) | |
| Biological_process | peptidyl-tyrosine dephosphorylation Inferred from electronic annotation. Source: GOC |
| Cellular_component | cleavage furrow Inferred from electronic annotation. Source: UniProtKB-SubCell cytoplasmInferred from electronic annotation. Source: UniProtKB-KW cytoskeletonInferred from electronic annotation. Source: UniProtKB-SubCell midbodyInferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular_function | DNA binding Inferred from electronic annotation. Source: InterPro protein tyrosine phosphatase activityInferred from electronic annotation. Source: EC protein tyrosine/serine/threonine phosphatase activityInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform 1 (identifier: Q6IVY4-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 2 (identifier: Q6IVY4-2) The sequence of this isoform differs from the canonical sequence as follows: 1-38: Missing. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 691 | 691 | Protein phosphatase Slingshot homolog | PRO_0000094848 | |||||
Regions | |||||||||
| Domain | 295 – 435 | 141 | Tyrosine-protein phosphatase | ||||||
| Coiled coil | 532 – 580 | 49 | Potential | ||||||
Sites | |||||||||
| Active site | 380 | 1 | Phosphocysteine intermediate Probable | ||||||
Natural variations | |||||||||
| Alternative sequence | 1 – 38 | 38 | Missing in isoform 2. | VSP_016338 | |||||
Experimental info | |||||||||
| Mutagenesis | 380 | 1 | C → S: Abrogates phosphatase activity. Ref.1 | ||||||
| Sequence conflict | 65 | 1 | P → R in AAT39429. Ref.1 | ||||||
| Sequence conflict | 624 | 1 | A → V in AAH80117. Ref.2 | ||||||
| Sequence conflict | 627 | 1 | A → K in AAH80117. Ref.2 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Functional involvement of Xenopus homologue of ADF/cofilin phosphatase, Slingshot (XSSH), in the gastrulation movement." Tanaka K., Nishio R., Haneda K., Abe H. Zool. Sci. 22:955-969(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, DEVELOPMENTAL STAGE, MUTAGENESIS OF CYS-380. |
| [2] | NIH - Xenopus Gene Collection (XGC) project Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Eye and Oocyte. |
| [3] | "Involvement of slingshot in the Rho-mediated dephosphorylation of ADF/cofilin during Xenopus cleavage." Tanaka K., Okubo Y., Abe H. Zool. Sci. 22:971-984(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH ACTIN, SUBCELLULAR LOCATION. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AY233266 mRNA. Translation: AAP57715.1. AY618876 mRNA. Translation: AAT39429.1. BC080117 mRNA. Translation: AAH80117.2. BC094472 mRNA. Translation: AAH94472.1. |
| RefSeq | NP_001082641.1. NM_001089172.1. |
| UniGene | Xl.2010. |
3D structure databases | |
| ProteinModelPortal | Q6IVY4. |
| SMR | Q6IVY4. Positions 296-436. |
| ModBase | Search... |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| GeneID | 398618. |
| KEGG | xla:398618. |
Organism-specific databases | |
| CTD | 54961. |
| Xenbase | XB-GENE-5816852. ssh3. |
Phylogenomic databases | |
| KO | K05766. |
Family and domain databases | |
| Gene3D | 1.10.10.60. 1 hit. |
| InterPro | IPR014876. DEK_C. IPR000340. Dual-sp_phosphatase_cat-dom. IPR020422. Dual-sp_phosphatase_subgr_cat. IPR024950. DUSP. IPR009057. Homeodomain-like. IPR027232. Ssh. IPR000387. Tyr/Dual-sp_Pase. IPR016130. Tyr_Pase_AS. [Graphical view] |
| PANTHER | PTHR10159. PTHR10159. 1 hit. PTHR10159:SF31. PTHR10159:SF31. 1 hit. |
| Pfam | PF08766. DEK_C. 1 hit. PF00782. DSPc. 1 hit. [Graphical view] |
| SMART | SM00195. DSPc. 1 hit. [Graphical view] |
| PROSITE | PS00383. TYR_PHOSPHATASE_1. 1 hit. PS50056. TYR_PHOSPHATASE_2. 1 hit. PS50054. TYR_PHOSPHATASE_DUAL. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | SSH_XENLA | ||||||||
| Accession | Primary (citable) accession number: Q6IVY4 Secondary accession number(s): Q505N4, Q68ET3, Q7T2T3 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
Relevant documents
| SIMILARITY comments Index of protein domains and families |