Sulfhydryl oxidase 1 precursor - Rattus norvegicus (Rat)

Names and origin

Protein names

Recommended name:
    Sulfhydryl oxidase 1
      Short name=rQSOX
      Short name=rSOx
    EC=1.8.3.2
Alternative name(s):
    Quiescin Q6
    FAD-dependent sulfhydryl oxidase-2
      Short name=SOx-2

Gene names

Name:	Qsox1
Synonyms:	Qscn6, Sox2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

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Protein attributes

Sequence length	750 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Catalyzes the oxidation of sulfhydryl groups in peptide and protein thiols to disulfides with the reduction of oxygen to hydrogen peroxide. May contribute to disulfide bond formation in a variety of secreted proteins. Ref.3
Catalytic activity	4 R'C(R)SH + O(2) = 2 R'C(R)S-S(R)CR' + 2 H(2)O.
Cofactor	Binds 1 FAD per subunit. Ref.1
Subunit structure	Monomer. Ref.1
Subcellular location	Isoform 1: Golgi apparatus membrane; Single-pass membrane proteinBy similarity. Isoform 2: SecretedBy similarity. Note= Found in the extracellular medium of quiescent cells but is not found in proliferating cells By similarity.
Tissue specificity	Isoform 1 is expressed in brain, hypophysis, heart, testis and the seminal vesicle. Isoform 3 is highly expressed in the seminal vesicles followed by testis, heart, brain, thymus, hypophysis and lung. Also expressed in prostate, kidney, spleen, liver. Ref.3 Ref.1
Sequence similarities	Belongs to the quiescin-sulfhydryl oxidase (QSOX) family. Contains 1 ERV/ALR sulfhydryl oxidase domain. Contains 1 thioredoxin domain.

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Ontologies

Keywords
Cellular component	Golgi apparatus Membrane Secreted
Coding sequence diversity	Alternative splicing
Domain	Signal Transmembrane
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
PTM	Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: InterPro protein thiol-disulfide exchange Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	extracellular space Inferred from sequence or structural similarity. Source: UniProtKB integral to Golgi membrane Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	flavin-linked sulfhydryl oxidase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

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Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q6IUU3-1) Also known as: QSOX-L; This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q6IUU3-2) The sequence of this isoform differs from the canonical sequence as follows: 27-32: Missing. 569-570: VM → LL 571-750: Missing.
Notes: Found in the extracellular medium of quiescent cells but is not found in proliferating cells (By similarity). No experimental confirmation available.

Isoform 3 (identifier: Q6IUU3-3) The sequence of this isoform differs from the canonical sequence as follows: 569-570: VM → LL 571-750: Missing.
Notes: No experimental confirmation available.

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 32

32

Ref.2

Chain

33 – 750

718

Sulfhydryl oxidase 1

PRO_0000249535

Regions

Transmembrane

710 – 730

21

Potential

Domain

33 – 159

127

Thioredoxin

Domain

399 – 506

108

ERV/ALR sulfhydryl oxidase

Amino acid modifications

Glycosylation

133

1

N-linked (GlcNAc...) Potential

Glycosylation

246

1

N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence

27 – 32

6

Missing in isoform 2.

VSP_020499

Alternative sequence

569 – 570

2

VM → LL in isoform 2 and isoform 3.

VSP_020497

Alternative sequence

571 – 750

180

Missing in isoform 2 and isoform 3.

VSP_020498

Experimental info

Sequence conflict

352

1

N → S in AAM67412. Ref.1

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Sequences

Sequence		Length	Mass (Da)
Isoform 1 (QSOX-L) [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 1781E657ED085114 Show »	FASTA	750	82,412
10 20 30 40 50 60 MRRCGRHSGP PSLLLLLLLL PPLLLSVPGA YAARLSVLYS SSDPLTLLDA DTVRPAVLGS 70 80 90 100 110 120 SSAWAVEFFA SWCGHCIAFA PTWKELANDV KDWRPALNLA VLDCADETNS AVCREFNIAG 130 140 150 160 170 180 FPTVRFFKAF SKNGTGTALP AAGANVQTLR MRLIDALESH RDTWPPACPP LEPAKLKDIN 190 200 210 220 230 240 EFFTRSKAEY LALIFEREDS YLGREVTLDL SQFHAVAVRR VLNSESDVVS KFAVTDFPSC 250 260 270 280 290 300 YLLLRNGSVS RVPVLVESRP FYTSYLRGLP GLTREAPPTT AAPVTPDKIA PTVWKFADRS 310 320 330 340 350 360 KIYMADLESA LHYILRVEVG KFSVLEGQRL VALKKFVAVL AKYFPGQPLV QNFLHSINDW 370 380 390 400 410 420 LQKQQKKKIP YSYFKAALDS RKENAVLAEK VNWIGCQGSE PHFRGFPCSL WVLFHFLTVQ 430 440 450 460 470 480 AHRYSEAHPQ EPADGQEVLQ AMRSYVQSFF GCRDCANHFE QMAAASMHQV KSPSNAVLWL 490 500 510 520 530 540 WTSHNRVNAR LSGALSEDPQ FPKVQWPPRE LCSACHNEVN GQVPLWDLGA TLNFLKAHFS 550 560 570 580 590 600 PANIVRDPPA PGPASRRGTQ DPEASPNLVM DTLKLETGNS VLGHEQAASA ASPGATALDV 610 620 630 640 650 660 PAGKPEASGP QELNAGLSMG GASPGQGPPE HTEELLRDVQ ENAQGQQHLS KRDTEALLLP 670 680 690 700 710 720 EVNHLQGPLA PRRGGHSPKQ LASILEGEPE ALAIQGRRQW LQVLGGGVSF LDISLCVGLY 730 740 750 SVSFMGLLAM YTYFRARMRT PKGHVSYPTA « Hide
Isoform 2. Checksum: B2E7B42708BD3B59 Show »		564	62,883
10 20 30 40 50 60 MRRCGRHSGP PSLLLLLLLL PPLLLSARLS VLYSSSDPLT LLDADTVRPA VLGSSSAWAV 70 80 90 100 110 120 EFFASWCGHC IAFAPTWKEL ANDVKDWRPA LNLAVLDCAD ETNSAVCREF NIAGFPTVRF 130 140 150 160 170 180 FKAFSKNGTG TALPAAGANV QTLRMRLIDA LESHRDTWPP ACPPLEPAKL KDINEFFTRS 190 200 210 220 230 240 KAEYLALIFE REDSYLGREV TLDLSQFHAV AVRRVLNSES DVVSKFAVTD FPSCYLLLRN 250 260 270 280 290 300 GSVSRVPVLV ESRPFYTSYL RGLPGLTREA PPTTAAPVTP DKIAPTVWKF ADRSKIYMAD 310 320 330 340 350 360 LESALHYILR VEVGKFSVLE GQRLVALKKF VAVLAKYFPG QPLVQNFLHS INDWLQKQQK 370 380 390 400 410 420 KKIPYSYFKA ALDSRKENAV LAEKVNWIGC QGSEPHFRGF PCSLWVLFHF LTVQAHRYSE 430 440 450 460 470 480 AHPQEPADGQ EVLQAMRSYV QSFFGCRDCA NHFEQMAAAS MHQVKSPSNA VLWLWTSHNR 490 500 510 520 530 540 VNARLSGALS EDPQFPKVQW PPRELCSACH NEVNGQVPLW DLGATLNFLK AHFSPANIVR 550 560 DPPAPGPASR RGTQDPEASP NLLL « Hide
Isoform 3. Checksum: FF53F8D2D1C7F4A9 Show »		570	63,442
10 20 30 40 50 60 MRRCGRHSGP PSLLLLLLLL PPLLLSVPGA YAARLSVLYS SSDPLTLLDA DTVRPAVLGS 70 80 90 100 110 120 SSAWAVEFFA SWCGHCIAFA PTWKELANDV KDWRPALNLA VLDCADETNS AVCREFNIAG 130 140 150 160 170 180 FPTVRFFKAF SKNGTGTALP AAGANVQTLR MRLIDALESH RDTWPPACPP LEPAKLKDIN 190 200 210 220 230 240 EFFTRSKAEY LALIFEREDS YLGREVTLDL SQFHAVAVRR VLNSESDVVS KFAVTDFPSC 250 260 270 280 290 300 YLLLRNGSVS RVPVLVESRP FYTSYLRGLP GLTREAPPTT AAPVTPDKIA PTVWKFADRS 310 320 330 340 350 360 KIYMADLESA LHYILRVEVG KFSVLEGQRL VALKKFVAVL AKYFPGQPLV QNFLHSINDW 370 380 390 400 410 420 LQKQQKKKIP YSYFKAALDS RKENAVLAEK VNWIGCQGSE PHFRGFPCSL WVLFHFLTVQ 430 440 450 460 470 480 AHRYSEAHPQ EPADGQEVLQ AMRSYVQSFF GCRDCANHFE QMAAASMHQV KSPSNAVLWL 490 500 510 520 530 540 WTSHNRVNAR LSGALSEDPQ FPKVQWPPRE LCSACHNEVN GQVPLWDLGA TLNFLKAHFS 550 560 570 PANIVRDPPA PGPASRRGTQ DPEASPNLLL « Hide

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References

[1]	"Rat seminal vesicle FAD-dependent sulfhydryl oxidase: biochemical characterization and molecular cloning of a member of the new sulfhydryl oxidase/quiescin Q6 gene family." Benayoun B., Esnard-Feve A., Castella S., Courty Y., Esnard F. J. Biol. Chem. 276:13830-13837(2001) [PubMed: 11278790] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2 AND 3), GLYCOSYLATION, TISSUE SPECIFICITY, COFACTOR, SUBUNIT. Strain: Wistar. Tissue: Seminal vesicle.
[2]	"Sulfhydryl oxidase (SOx) from mouse epidermis: molecular cloning, nucleotide sequence, and expression of recombinant protein in the cultured cells." Matsuba S., Suga Y., Ishidoh K., Hashimoto Y., Takamori K., Kominami E., Wilhelm B., Seitz J., Ogawa H. J. Dermatol. Sci. 30:50-62(2002) [PubMed: 12354420] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), PROTEIN SEQUENCE OF 33-65, MASS SPECTROMETRY.
[3]	"Identification and expression of a new splicing variant of FAD-sulfhydryl oxidase in adult rat brain." Radom J., Colin D., Thiebault F., Dognin-Bergeret M.J., Mairet-Coello G., Esnard-Feve A., Fellmann D., Jouvenot M. Biochim. Biophys. Acta 1759:225-233(2006) [PubMed: 16806532] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, MASS SPECTROMETRY. Strain: Wistar. Tissue: Brain.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	AF217799 mRNA. Translation: AAM67412.1. AF285078 mRNA. Translation: AAG53892.1. AB044285 mRNA. Translation: BAB21937.1. AY623665 mRNA. Translation: AAT40988.1.
RefSeq	NP_001103368.1. NP_445883.1.
UniGene	Rn.44920
3D structure databases
HSSP	HSSP built from PDB template 1DBY based on UniProtKB P23400.
ModBase	Search...
Genome annotation databases
Ensembl	ENSRNOG00000003649. Rattus norvegicus. [Contig view]
GeneID	84491.
KEGG	rno:84491.
Organism-specific databases
RGD	68957. Qscn6.
Phylogenomic databases
HOVERGEN	Q6IUU3.
Gene expression databases
ArrayExpress	Q6IUU3.
GermOnline	ENSRNOG00000003649. Rattus norvegicus.
Family and domain databases
InterPro	IPR006863. Evr1_Alr. IPR006662. Thioredoxin-like. IPR013766. Thioredoxin_dom. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:1.20.120.310. Evr1_Alr. 1 hit. G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
Pfam	PF04777. Evr1_Alr. 1 hit. PF00085. Thioredoxin. 1 hit. [Graphical view]
PRINTS	PR00421. THIOREDOXIN.
PROSITE	PS51324. ERV_ALR. 1 hit. PS51352. THIOREDOXIN_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	617042.

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Entry information

Entry name

QSOX1_RAT

Accession

Primary (citable) accession number: Q6IUU3
Secondary accession number(s): Q8K4M2, Q99J80

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2006
Last sequence update:	July 5, 2004
Last modified:	November 25, 2008
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Keywords

Gene Ontology (GO)

Alternative products

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Natural variations

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents