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Q6IUU3

- QSOX1_RAT

UniProt

Q6IUU3 - QSOX1_RAT

Protein

Sulfhydryl oxidase 1

Gene

Qsox1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Catalyzes the oxidation of sulfhydryl groups in peptide and protein thiols to disulfides with the reduction of oxygen to hydrogen peroxide. May contribute to disulfide bond formation in a variety of secreted proteins.1 Publication

    Catalytic activityi

    2 R'C(R)SH + O2 = R'C(R)S-S(R)CR' + H2O2.

    Cofactori

    Binds 1 FAD per subunit.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei73 – 731NucleophileBy similarity
    Active sitei76 – 761NucleophileBy similarity
    Binding sitei404 – 4041FADBy similarity
    Binding sitei411 – 4111FADBy similarity
    Binding sitei415 – 4151FADBy similarity
    Binding sitei454 – 4541FADBy similarity
    Binding sitei458 – 4581FADBy similarity
    Binding sitei503 – 5031FADBy similarity
    Binding sitei506 – 5061FADBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi481 – 4888FADBy similarity

    GO - Molecular functioni

    1. flavin-linked sulfhydryl oxidase activity Source: UniProtKB
    2. protein disulfide isomerase activity Source: UniProtKB

    GO - Biological processi

    1. cell redox homeostasis Source: InterPro

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    FAD, Flavoprotein

    Enzyme and pathway databases

    BRENDAi1.8.3.2. 5301.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sulfhydryl oxidase 1 (EC:1.8.3.2)
    Short name:
    rQSOX
    Short name:
    rSOx
    Alternative name(s):
    FAD-dependent sulfhydryl oxidase-2
    Short name:
    SOx-2
    Quiescin Q6
    Gene namesi
    Name:Qsox1
    Synonyms:Qscn6, Sox2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 13

    Organism-specific databases

    RGDi68957. Qsox1.

    Subcellular locationi

    Isoform 2 : Secreted By similarity
    Note: Found in the extracellular medium of quiescent cells but is not found in proliferating cells.By similarity

    GO - Cellular componenti

    1. extracellular space Source: UniProtKB
    2. integral component of Golgi membrane Source: UniProtKB

    Keywords - Cellular componenti

    Golgi apparatus, Membrane, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 32321 PublicationAdd
    BLAST
    Chaini33 – 750718Sulfhydryl oxidase 1PRO_0000249535Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi73 ↔ 76Redox-activePROSITE-ProRule annotation
    Glycosylationi133 – 1331N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi246 – 2461N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi396 ↔ 408PROSITE-ProRule annotation
    Disulfide bondi452 ↔ 455PROSITE-ProRule annotation
    Disulfide bondi512 ↔ 515PROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiQ6IUU3.
    PRIDEiQ6IUU3.

    Expressioni

    Tissue specificityi

    Isoform 1 is expressed in brain, hypophysis, heart, testis and the seminal vesicle. Isoform 3 is highly expressed in the seminal vesicles followed by testis, heart, brain, thymus, hypophysis and lung. Also expressed in prostate, kidney, spleen, liver.2 Publications

    Gene expression databases

    GenevestigatoriQ6IUU3.

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ6IUU3.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei710 – 73021HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini33 – 159127ThioredoxinPROSITE-ProRule annotationAdd
    BLAST
    Domaini399 – 506108ERV/ALR sulfhydryl oxidasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 ERV/ALR sulfhydryl oxidase domain.PROSITE-ProRule annotation
    Contains 1 thioredoxin domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0526.
    GeneTreeiENSGT00390000008045.
    HOGENOMiHOG000231631.
    HOVERGENiHBG080360.
    InParanoidiQ6IUU3.
    KOiK10758.
    OMAiSHNRVNA.
    OrthoDBiEOG73RB9X.
    PhylomeDBiQ6IUU3.

    Family and domain databases

    Gene3Di1.20.120.310. 1 hit.
    3.40.30.10. 1 hit.
    InterProiIPR017905. ERV/ALR_sulphydryl_oxidase.
    IPR012336. Thioredoxin-like_fold.
    IPR013766. Thioredoxin_domain.
    [Graphical view]
    PfamiPF04777. Evr1_Alr. 1 hit.
    PF00085. Thioredoxin. 1 hit.
    [Graphical view]
    SUPFAMiSSF52833. SSF52833. 1 hit.
    SSF69000. SSF69000. 1 hit.
    PROSITEiPS51324. ERV_ALR. 1 hit.
    PS51352. THIOREDOXIN_2. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q6IUU3-1) [UniParc]FASTAAdd to Basket

    Also known as: QSOX-L

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MRRCGRHSGP PSLLLLLLLL PPLLLSVPGA YAARLSVLYS SSDPLTLLDA    50
    DTVRPAVLGS SSAWAVEFFA SWCGHCIAFA PTWKELANDV KDWRPALNLA 100
    VLDCADETNS AVCREFNIAG FPTVRFFKAF SKNGTGTALP AAGANVQTLR 150
    MRLIDALESH RDTWPPACPP LEPAKLKDIN EFFTRSKAEY LALIFEREDS 200
    YLGREVTLDL SQFHAVAVRR VLNSESDVVS KFAVTDFPSC YLLLRNGSVS 250
    RVPVLVESRP FYTSYLRGLP GLTREAPPTT AAPVTPDKIA PTVWKFADRS 300
    KIYMADLESA LHYILRVEVG KFSVLEGQRL VALKKFVAVL AKYFPGQPLV 350
    QNFLHSINDW LQKQQKKKIP YSYFKAALDS RKENAVLAEK VNWIGCQGSE 400
    PHFRGFPCSL WVLFHFLTVQ AHRYSEAHPQ EPADGQEVLQ AMRSYVQSFF 450
    GCRDCANHFE QMAAASMHQV KSPSNAVLWL WTSHNRVNAR LSGALSEDPQ 500
    FPKVQWPPRE LCSACHNEVN GQVPLWDLGA TLNFLKAHFS PANIVRDPPA 550
    PGPASRRGTQ DPEASPNLVM DTLKLETGNS VLGHEQAASA ASPGATALDV 600
    PAGKPEASGP QELNAGLSMG GASPGQGPPE HTEELLRDVQ ENAQGQQHLS 650
    KRDTEALLLP EVNHLQGPLA PRRGGHSPKQ LASILEGEPE ALAIQGRRQW 700
    LQVLGGGVSF LDISLCVGLY SVSFMGLLAM YTYFRARMRT PKGHVSYPTA 750
    Length:750
    Mass (Da):82,412
    Last modified:July 5, 2004 - v1
    Checksum:i1781E657ED085114
    GO
    Isoform 2 (identifier: Q6IUU3-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         27-32: Missing.
         569-570: VM → LL
         571-750: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:564
    Mass (Da):62,883
    Checksum:iB2E7B42708BD3B59
    GO
    Isoform 3 (identifier: Q6IUU3-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         569-570: VM → LL
         571-750: Missing.

    Show »
    Length:570
    Mass (Da):63,442
    Checksum:iFF53F8D2D1C7F4A9
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti352 – 3521N → S in AAM67412. (PubMed:11278790)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei27 – 326Missing in isoform 2. 1 PublicationVSP_020499
    Alternative sequencei569 – 5702VM → LL in isoform 2 and isoform 3. 2 PublicationsVSP_020497
    Alternative sequencei571 – 750180Missing in isoform 2 and isoform 3. 2 PublicationsVSP_020498Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF217799 mRNA. Translation: AAM67412.1.
    AF285078 mRNA. Translation: AAG53892.1.
    AB044285 mRNA. Translation: BAB21937.1.
    AY623665 mRNA. Translation: AAT40988.1.
    RefSeqiNP_001103368.1. NM_001109898.1. [Q6IUU3-1]
    NP_445883.1. NM_053431.3. [Q6IUU3-3]
    UniGeneiRn.44920.

    Genome annotation databases

    EnsembliENSRNOT00000005052; ENSRNOP00000005052; ENSRNOG00000003649. [Q6IUU3-3]
    ENSRNOT00000068044; ENSRNOP00000061072; ENSRNOG00000003649. [Q6IUU3-2]
    GeneIDi84491.
    KEGGirno:84491.
    UCSCiRGD:68957. rat. [Q6IUU3-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF217799 mRNA. Translation: AAM67412.1 .
    AF285078 mRNA. Translation: AAG53892.1 .
    AB044285 mRNA. Translation: BAB21937.1 .
    AY623665 mRNA. Translation: AAT40988.1 .
    RefSeqi NP_001103368.1. NM_001109898.1. [Q6IUU3-1 ]
    NP_445883.1. NM_053431.3. [Q6IUU3-3 ]
    UniGenei Rn.44920.

    3D structure databases

    ProteinModelPortali Q6IUU3.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi Q6IUU3.
    PRIDEi Q6IUU3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000005052 ; ENSRNOP00000005052 ; ENSRNOG00000003649 . [Q6IUU3-3 ]
    ENSRNOT00000068044 ; ENSRNOP00000061072 ; ENSRNOG00000003649 . [Q6IUU3-2 ]
    GeneIDi 84491.
    KEGGi rno:84491.
    UCSCi RGD:68957. rat. [Q6IUU3-1 ]

    Organism-specific databases

    CTDi 5768.
    RGDi 68957. Qsox1.

    Phylogenomic databases

    eggNOGi COG0526.
    GeneTreei ENSGT00390000008045.
    HOGENOMi HOG000231631.
    HOVERGENi HBG080360.
    InParanoidi Q6IUU3.
    KOi K10758.
    OMAi SHNRVNA.
    OrthoDBi EOG73RB9X.
    PhylomeDBi Q6IUU3.

    Enzyme and pathway databases

    BRENDAi 1.8.3.2. 5301.

    Miscellaneous databases

    NextBioi 617042.
    PROi Q6IUU3.

    Gene expression databases

    Genevestigatori Q6IUU3.

    Family and domain databases

    Gene3Di 1.20.120.310. 1 hit.
    3.40.30.10. 1 hit.
    InterProi IPR017905. ERV/ALR_sulphydryl_oxidase.
    IPR012336. Thioredoxin-like_fold.
    IPR013766. Thioredoxin_domain.
    [Graphical view ]
    Pfami PF04777. Evr1_Alr. 1 hit.
    PF00085. Thioredoxin. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52833. SSF52833. 1 hit.
    SSF69000. SSF69000. 1 hit.
    PROSITEi PS51324. ERV_ALR. 1 hit.
    PS51352. THIOREDOXIN_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Rat seminal vesicle FAD-dependent sulfhydryl oxidase: biochemical characterization and molecular cloning of a member of the new sulfhydryl oxidase/quiescin Q6 gene family."
      Benayoun B., Esnard-Feve A., Castella S., Courty Y., Esnard F.
      J. Biol. Chem. 276:13830-13837(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2 AND 3), GLYCOSYLATION, TISSUE SPECIFICITY, COFACTOR, SUBUNIT.
      Strain: Wistar.
      Tissue: Seminal vesicle.
    2. "Sulfhydryl oxidase (SOx) from mouse epidermis: molecular cloning, nucleotide sequence, and expression of recombinant protein in the cultured cells."
      Matsuba S., Suga Y., Ishidoh K., Hashimoto Y., Takamori K., Kominami E., Wilhelm B., Seitz J., Ogawa H.
      J. Dermatol. Sci. 30:50-62(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), PROTEIN SEQUENCE OF 33-65, IDENTIFICATION BY MASS SPECTROMETRY.
    3. "Identification and expression of a new splicing variant of FAD-sulfhydryl oxidase in adult rat brain."
      Radom J., Colin D., Thiebault F., Dognin-Bergeret M.J., Mairet-Coello G., Esnard-Feve A., Fellmann D., Jouvenot M.
      Biochim. Biophys. Acta 1759:225-233(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Wistar.
      Tissue: Brain.

    Entry informationi

    Entry nameiQSOX1_RAT
    AccessioniPrimary (citable) accession number: Q6IUU3
    Secondary accession number(s): Q8K4M2, Q99J80
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 19, 2006
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 84 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3