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Protein

Sulfhydryl oxidase 1

Gene

Qsox1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the oxidation of sulfhydryl groups in peptide and protein thiols to disulfides with the reduction of oxygen to hydrogen peroxide. May contribute to disulfide bond formation in a variety of secreted proteins.1 Publication

Catalytic activityi

2 R'C(R)SH + O2 = R'C(R)S-S(R)CR' + H2O2.

Cofactori

FAD1 PublicationNote: Binds 1 FAD per subunit.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei73NucleophileBy similarity1
Active sitei76NucleophileBy similarity1
Binding sitei404FADBy similarity1
Binding sitei411FADBy similarity1
Binding sitei415FADBy similarity1
Binding sitei454FADBy similarity1
Binding sitei458FADBy similarity1
Binding sitei503FADBy similarity1
Binding sitei506FADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi481 – 488FADBy similarity8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BRENDAi1.8.3.2. 5301.
ReactomeiR-RNO-114608. Platelet degranulation.
R-RNO-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Sulfhydryl oxidase 1 (EC:1.8.3.2)
Short name:
rQSOX
Short name:
rSOx
Alternative name(s):
FAD-dependent sulfhydryl oxidase-2
Short name:
SOx-2
Quiescin Q6
Gene namesi
Name:Qsox1
Synonyms:Qscn6, Sox2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 13

Organism-specific databases

RGDi68957. Qsox1.

Subcellular locationi

Isoform 2 :
  • Secreted By similarity

  • Note: Found in the extracellular medium of quiescent cells but is not found in proliferating cells.By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei710 – 730HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 321 PublicationAdd BLAST32
ChainiPRO_000024953533 – 750Sulfhydryl oxidase 1Add BLAST718

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi73 ↔ 76Redox-activePROSITE-ProRule annotation
Glycosylationi133N-linked (GlcNAc...)Sequence analysis1
Glycosylationi246N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi396 ↔ 408PROSITE-ProRule annotation
Disulfide bondi452 ↔ 455PROSITE-ProRule annotation
Disulfide bondi512 ↔ 515PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ6IUU3.
PRIDEiQ6IUU3.

Expressioni

Tissue specificityi

Isoform 1 is expressed in brain, hypophysis, heart, testis and the seminal vesicle. Isoform 3 is highly expressed in the seminal vesicles followed by testis, heart, brain, thymus, hypophysis and lung. Also expressed in prostate, kidney, spleen, liver.2 Publications

Gene expression databases

BgeeiENSRNOG00000003649.
GenevisibleiQ6IUU3. RN.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000005052.

Structurei

Secondary structure

1750
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi41 – 48Combined sources8
Turni50 – 52Combined sources3
Helixi53 – 57Combined sources5
Beta strandi61 – 69Combined sources9
Helixi74 – 89Combined sources16
Helixi91 – 93Combined sources3
Turni94 – 96Combined sources3
Beta strandi97 – 103Combined sources7
Helixi107 – 109Combined sources3
Helixi110 – 115Combined sources6
Beta strandi120 – 127Combined sources8
Helixi146 – 157Combined sources12
Helixi176 – 180Combined sources5
Turni181 – 185Combined sources5
Beta strandi189 – 196Combined sources8
Helixi202 – 209Combined sources8
Turni210 – 212Combined sources3
Beta strandi216 – 222Combined sources7
Helixi226 – 231Combined sources6
Beta strandi237 – 244Combined sources8
Beta strandi249 – 251Combined sources3
Beta strandi255 – 258Combined sources4
Helixi259 – 266Combined sources8
Helixi293 – 296Combined sources4
Beta strandi301 – 303Combined sources3
Helixi304 – 316Combined sources13
Helixi319 – 321Combined sources3
Beta strandi323 – 325Combined sources3
Helixi327 – 343Combined sources17
Helixi348 – 363Combined sources16
Helixi371 – 380Combined sources10
Helixi383 – 386Combined sources4
Beta strandi403 – 405Combined sources3
Helixi406 – 427Combined sources22
Helixi437 – 448Combined sources12
Helixi453 – 466Combined sources14
Helixi467 – 469Combined sources3
Helixi473 – 490Combined sources18
Beta strandi505 – 507Combined sources3
Turni509 – 511Combined sources3
Beta strandi516 – 519Combined sources4
Helixi528 – 538Combined sources11
Helixi541 – 543Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4P2LX-ray2.90A/B36-550[»]
ProteinModelPortaliQ6IUU3.
SMRiQ6IUU3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini33 – 159ThioredoxinPROSITE-ProRule annotationAdd BLAST127
Domaini399 – 506ERV/ALR sulfhydryl oxidasePROSITE-ProRule annotationAdd BLAST108

Sequence similaritiesi

Contains 1 ERV/ALR sulfhydryl oxidase domain.PROSITE-ProRule annotation
Contains 1 thioredoxin domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1731. Eukaryota.
ENOG410XVJT. LUCA.
GeneTreeiENSGT00390000008045.
HOGENOMiHOG000231631.
HOVERGENiHBG080360.
InParanoidiQ6IUU3.
KOiK10758.
OMAiKNFFGCT.
OrthoDBiEOG091G038J.
PhylomeDBiQ6IUU3.

Family and domain databases

Gene3Di1.20.120.310. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR017905. ERV/ALR_sulphydryl_oxidase.
IPR012336. Thioredoxin-like_fold.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF04777. Evr1_Alr. 1 hit.
PF00085. Thioredoxin. 1 hit.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 1 hit.
SSF69000. SSF69000. 1 hit.
PROSITEiPS51324. ERV_ALR. 1 hit.
PS51352. THIOREDOXIN_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6IUU3-1) [UniParc]FASTAAdd to basket
Also known as: QSOX-L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRRCGRHSGP PSLLLLLLLL PPLLLSVPGA YAARLSVLYS SSDPLTLLDA
60 70 80 90 100
DTVRPAVLGS SSAWAVEFFA SWCGHCIAFA PTWKELANDV KDWRPALNLA
110 120 130 140 150
VLDCADETNS AVCREFNIAG FPTVRFFKAF SKNGTGTALP AAGANVQTLR
160 170 180 190 200
MRLIDALESH RDTWPPACPP LEPAKLKDIN EFFTRSKAEY LALIFEREDS
210 220 230 240 250
YLGREVTLDL SQFHAVAVRR VLNSESDVVS KFAVTDFPSC YLLLRNGSVS
260 270 280 290 300
RVPVLVESRP FYTSYLRGLP GLTREAPPTT AAPVTPDKIA PTVWKFADRS
310 320 330 340 350
KIYMADLESA LHYILRVEVG KFSVLEGQRL VALKKFVAVL AKYFPGQPLV
360 370 380 390 400
QNFLHSINDW LQKQQKKKIP YSYFKAALDS RKENAVLAEK VNWIGCQGSE
410 420 430 440 450
PHFRGFPCSL WVLFHFLTVQ AHRYSEAHPQ EPADGQEVLQ AMRSYVQSFF
460 470 480 490 500
GCRDCANHFE QMAAASMHQV KSPSNAVLWL WTSHNRVNAR LSGALSEDPQ
510 520 530 540 550
FPKVQWPPRE LCSACHNEVN GQVPLWDLGA TLNFLKAHFS PANIVRDPPA
560 570 580 590 600
PGPASRRGTQ DPEASPNLVM DTLKLETGNS VLGHEQAASA ASPGATALDV
610 620 630 640 650
PAGKPEASGP QELNAGLSMG GASPGQGPPE HTEELLRDVQ ENAQGQQHLS
660 670 680 690 700
KRDTEALLLP EVNHLQGPLA PRRGGHSPKQ LASILEGEPE ALAIQGRRQW
710 720 730 740 750
LQVLGGGVSF LDISLCVGLY SVSFMGLLAM YTYFRARMRT PKGHVSYPTA
Length:750
Mass (Da):82,412
Last modified:July 5, 2004 - v1
Checksum:i1781E657ED085114
GO
Isoform 2 (identifier: Q6IUU3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     27-32: Missing.
     569-570: VM → LL
     571-750: Missing.

Note: No experimental confirmation available.
Show »
Length:564
Mass (Da):62,883
Checksum:iB2E7B42708BD3B59
GO
Isoform 3 (identifier: Q6IUU3-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     569-570: VM → LL
     571-750: Missing.

Show »
Length:570
Mass (Da):63,442
Checksum:iFF53F8D2D1C7F4A9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti352N → S in AAM67412 (PubMed:11278790).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_02049927 – 32Missing in isoform 2. 1 Publication6
Alternative sequenceiVSP_020497569 – 570VM → LL in isoform 2 and isoform 3. 2 Publications2
Alternative sequenceiVSP_020498571 – 750Missing in isoform 2 and isoform 3. 2 PublicationsAdd BLAST180

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF217799 mRNA. Translation: AAM67412.1.
AF285078 mRNA. Translation: AAG53892.1.
AB044285 mRNA. Translation: BAB21937.1.
AY623665 mRNA. Translation: AAT40988.1.
RefSeqiNP_001103368.1. NM_001109898.1. [Q6IUU3-1]
NP_445883.1. NM_053431.3. [Q6IUU3-3]
UniGeneiRn.44920.

Genome annotation databases

EnsembliENSRNOT00000005052; ENSRNOP00000005052; ENSRNOG00000003649. [Q6IUU3-3]
ENSRNOT00000068044; ENSRNOP00000061072; ENSRNOG00000003649. [Q6IUU3-1]
GeneIDi84491.
KEGGirno:84491.
UCSCiRGD:68957. rat. [Q6IUU3-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF217799 mRNA. Translation: AAM67412.1.
AF285078 mRNA. Translation: AAG53892.1.
AB044285 mRNA. Translation: BAB21937.1.
AY623665 mRNA. Translation: AAT40988.1.
RefSeqiNP_001103368.1. NM_001109898.1. [Q6IUU3-1]
NP_445883.1. NM_053431.3. [Q6IUU3-3]
UniGeneiRn.44920.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4P2LX-ray2.90A/B36-550[»]
ProteinModelPortaliQ6IUU3.
SMRiQ6IUU3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000005052.

Proteomic databases

PaxDbiQ6IUU3.
PRIDEiQ6IUU3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000005052; ENSRNOP00000005052; ENSRNOG00000003649. [Q6IUU3-3]
ENSRNOT00000068044; ENSRNOP00000061072; ENSRNOG00000003649. [Q6IUU3-1]
GeneIDi84491.
KEGGirno:84491.
UCSCiRGD:68957. rat. [Q6IUU3-1]

Organism-specific databases

CTDi5768.
RGDi68957. Qsox1.

Phylogenomic databases

eggNOGiKOG1731. Eukaryota.
ENOG410XVJT. LUCA.
GeneTreeiENSGT00390000008045.
HOGENOMiHOG000231631.
HOVERGENiHBG080360.
InParanoidiQ6IUU3.
KOiK10758.
OMAiKNFFGCT.
OrthoDBiEOG091G038J.
PhylomeDBiQ6IUU3.

Enzyme and pathway databases

BRENDAi1.8.3.2. 5301.
ReactomeiR-RNO-114608. Platelet degranulation.
R-RNO-6798695. Neutrophil degranulation.

Miscellaneous databases

PROiQ6IUU3.

Gene expression databases

BgeeiENSRNOG00000003649.
GenevisibleiQ6IUU3. RN.

Family and domain databases

Gene3Di1.20.120.310. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR017905. ERV/ALR_sulphydryl_oxidase.
IPR012336. Thioredoxin-like_fold.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF04777. Evr1_Alr. 1 hit.
PF00085. Thioredoxin. 1 hit.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 1 hit.
SSF69000. SSF69000. 1 hit.
PROSITEiPS51324. ERV_ALR. 1 hit.
PS51352. THIOREDOXIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiQSOX1_RAT
AccessioniPrimary (citable) accession number: Q6IUU3
Secondary accession number(s): Q8K4M2, Q99J80
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: July 5, 2004
Last modified: November 30, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.