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Q6IUU3

- QSOX1_RAT

UniProt

Q6IUU3 - QSOX1_RAT

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Protein
Sulfhydryl oxidase 1
Gene
Qsox1, Qscn6, Sox2
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the oxidation of sulfhydryl groups in peptide and protein thiols to disulfides with the reduction of oxygen to hydrogen peroxide. May contribute to disulfide bond formation in a variety of secreted proteins.1 Publication

Catalytic activityi

2 R'C(R)SH + O2 = R'C(R)S-S(R)CR' + H2O2.

Cofactori

Binds 1 FAD per subunit.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei73 – 731Nucleophile By similarity
Active sitei76 – 761Nucleophile By similarity
Binding sitei404 – 4041FAD By similarity
Binding sitei411 – 4111FAD By similarity
Binding sitei415 – 4151FAD By similarity
Binding sitei454 – 4541FAD By similarity
Binding sitei458 – 4581FAD By similarity
Binding sitei503 – 5031FAD By similarity
Binding sitei506 – 5061FAD By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi481 – 4888FAD By similarity

GO - Molecular functioni

  1. flavin-linked sulfhydryl oxidase activity Source: UniProtKB
  2. protein disulfide isomerase activity Source: UniProtKB
Complete GO annotation...

GO - Biological processi

  1. cell redox homeostasis Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BRENDAi1.8.3.2. 5301.

Names & Taxonomyi

Protein namesi
Recommended name:
Sulfhydryl oxidase 1 (EC:1.8.3.2)
Short name:
rQSOX
Short name:
rSOx
Alternative name(s):
FAD-dependent sulfhydryl oxidase-2
Short name:
SOx-2
Quiescin Q6
Gene namesi
Name:Qsox1
Synonyms:Qscn6, Sox2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 13

Organism-specific databases

RGDi68957. Qsox1.

Subcellular locationi

Isoform 2 : Secreted By similarity
Note: Found in the extracellular medium of quiescent cells but is not found in proliferating cells By similarity.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei710 – 73021Helical; Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. extracellular space Source: UniProtKB
  2. integral component of Golgi membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 32321 Publication
Add
BLAST
Chaini33 – 750718Sulfhydryl oxidase 1
PRO_0000249535Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi73 ↔ 76Redox-active By similarity
Glycosylationi133 – 1331N-linked (GlcNAc...) Reviewed prediction
Glycosylationi246 – 2461N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi396 ↔ 408 By similarity
Disulfide bondi452 ↔ 455 By similarity
Disulfide bondi512 ↔ 515 By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ6IUU3.
PRIDEiQ6IUU3.

Expressioni

Tissue specificityi

Isoform 1 is expressed in brain, hypophysis, heart, testis and the seminal vesicle. Isoform 3 is highly expressed in the seminal vesicles followed by testis, heart, brain, thymus, hypophysis and lung. Also expressed in prostate, kidney, spleen, liver.2 Publications

Gene expression databases

GenevestigatoriQ6IUU3.

Interactioni

Subunit structurei

Monomer.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ6IUU3.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini33 – 159127Thioredoxin
Add
BLAST
Domaini399 – 506108ERV/ALR sulfhydryl oxidase
Add
BLAST

Sequence similaritiesi

Contains 1 thioredoxin domain.

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0526.
GeneTreeiENSGT00390000008045.
HOGENOMiHOG000231631.
HOVERGENiHBG080360.
InParanoidiQ6IUU3.
KOiK10758.
OMAiSHNRVNA.
OrthoDBiEOG73RB9X.
PhylomeDBiQ6IUU3.

Family and domain databases

Gene3Di1.20.120.310. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR017905. ERV/ALR_sulphydryl_oxidase.
IPR012336. Thioredoxin-like_fold.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF04777. Evr1_Alr. 1 hit.
PF00085. Thioredoxin. 1 hit.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 1 hit.
SSF69000. SSF69000. 1 hit.
PROSITEiPS51324. ERV_ALR. 1 hit.
PS51352. THIOREDOXIN_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q6IUU3-1) [UniParc]FASTAAdd to Basket

Also known as: QSOX-L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MRRCGRHSGP PSLLLLLLLL PPLLLSVPGA YAARLSVLYS SSDPLTLLDA    50
DTVRPAVLGS SSAWAVEFFA SWCGHCIAFA PTWKELANDV KDWRPALNLA 100
VLDCADETNS AVCREFNIAG FPTVRFFKAF SKNGTGTALP AAGANVQTLR 150
MRLIDALESH RDTWPPACPP LEPAKLKDIN EFFTRSKAEY LALIFEREDS 200
YLGREVTLDL SQFHAVAVRR VLNSESDVVS KFAVTDFPSC YLLLRNGSVS 250
RVPVLVESRP FYTSYLRGLP GLTREAPPTT AAPVTPDKIA PTVWKFADRS 300
KIYMADLESA LHYILRVEVG KFSVLEGQRL VALKKFVAVL AKYFPGQPLV 350
QNFLHSINDW LQKQQKKKIP YSYFKAALDS RKENAVLAEK VNWIGCQGSE 400
PHFRGFPCSL WVLFHFLTVQ AHRYSEAHPQ EPADGQEVLQ AMRSYVQSFF 450
GCRDCANHFE QMAAASMHQV KSPSNAVLWL WTSHNRVNAR LSGALSEDPQ 500
FPKVQWPPRE LCSACHNEVN GQVPLWDLGA TLNFLKAHFS PANIVRDPPA 550
PGPASRRGTQ DPEASPNLVM DTLKLETGNS VLGHEQAASA ASPGATALDV 600
PAGKPEASGP QELNAGLSMG GASPGQGPPE HTEELLRDVQ ENAQGQQHLS 650
KRDTEALLLP EVNHLQGPLA PRRGGHSPKQ LASILEGEPE ALAIQGRRQW 700
LQVLGGGVSF LDISLCVGLY SVSFMGLLAM YTYFRARMRT PKGHVSYPTA 750
Length:750
Mass (Da):82,412
Last modified:July 5, 2004 - v1
Checksum:i1781E657ED085114
GO
Isoform 2 (identifier: Q6IUU3-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     27-32: Missing.
     569-570: VM → LL
     571-750: Missing.

Note: No experimental confirmation available.

Show »
Length:564
Mass (Da):62,883
Checksum:iB2E7B42708BD3B59
GO
Isoform 3 (identifier: Q6IUU3-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     569-570: VM → LL
     571-750: Missing.

Show »
Length:570
Mass (Da):63,442
Checksum:iFF53F8D2D1C7F4A9
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei27 – 326Missing in isoform 2.
VSP_020499
Alternative sequencei569 – 5702VM → LL in isoform 2 and isoform 3.
VSP_020497
Alternative sequencei571 – 750180Missing in isoform 2 and isoform 3.
VSP_020498Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti352 – 3521N → S in AAM67412. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF217799 mRNA. Translation: AAM67412.1.
AF285078 mRNA. Translation: AAG53892.1.
AB044285 mRNA. Translation: BAB21937.1.
AY623665 mRNA. Translation: AAT40988.1.
RefSeqiNP_001103368.1. NM_001109898.1. [Q6IUU3-1]
NP_445883.1. NM_053431.3. [Q6IUU3-3]
UniGeneiRn.44920.

Genome annotation databases

EnsembliENSRNOT00000005052; ENSRNOP00000005052; ENSRNOG00000003649. [Q6IUU3-3]
ENSRNOT00000068044; ENSRNOP00000061072; ENSRNOG00000003649. [Q6IUU3-2]
GeneIDi84491.
KEGGirno:84491.
UCSCiRGD:68957. rat. [Q6IUU3-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF217799 mRNA. Translation: AAM67412.1 .
AF285078 mRNA. Translation: AAG53892.1 .
AB044285 mRNA. Translation: BAB21937.1 .
AY623665 mRNA. Translation: AAT40988.1 .
RefSeqi NP_001103368.1. NM_001109898.1. [Q6IUU3-1 ]
NP_445883.1. NM_053431.3. [Q6IUU3-3 ]
UniGenei Rn.44920.

3D structure databases

ProteinModelPortali Q6IUU3.
ModBasei Search...

Proteomic databases

PaxDbi Q6IUU3.
PRIDEi Q6IUU3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000005052 ; ENSRNOP00000005052 ; ENSRNOG00000003649 . [Q6IUU3-3 ]
ENSRNOT00000068044 ; ENSRNOP00000061072 ; ENSRNOG00000003649 . [Q6IUU3-2 ]
GeneIDi 84491.
KEGGi rno:84491.
UCSCi RGD:68957. rat. [Q6IUU3-1 ]

Organism-specific databases

CTDi 5768.
RGDi 68957. Qsox1.

Phylogenomic databases

eggNOGi COG0526.
GeneTreei ENSGT00390000008045.
HOGENOMi HOG000231631.
HOVERGENi HBG080360.
InParanoidi Q6IUU3.
KOi K10758.
OMAi SHNRVNA.
OrthoDBi EOG73RB9X.
PhylomeDBi Q6IUU3.

Enzyme and pathway databases

BRENDAi 1.8.3.2. 5301.

Miscellaneous databases

NextBioi 617042.
PROi Q6IUU3.

Gene expression databases

Genevestigatori Q6IUU3.

Family and domain databases

Gene3Di 1.20.120.310. 1 hit.
3.40.30.10. 1 hit.
InterProi IPR017905. ERV/ALR_sulphydryl_oxidase.
IPR012336. Thioredoxin-like_fold.
IPR013766. Thioredoxin_domain.
[Graphical view ]
Pfami PF04777. Evr1_Alr. 1 hit.
PF00085. Thioredoxin. 1 hit.
[Graphical view ]
SUPFAMi SSF52833. SSF52833. 1 hit.
SSF69000. SSF69000. 1 hit.
PROSITEi PS51324. ERV_ALR. 1 hit.
PS51352. THIOREDOXIN_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Rat seminal vesicle FAD-dependent sulfhydryl oxidase: biochemical characterization and molecular cloning of a member of the new sulfhydryl oxidase/quiescin Q6 gene family."
    Benayoun B., Esnard-Feve A., Castella S., Courty Y., Esnard F.
    J. Biol. Chem. 276:13830-13837(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2 AND 3), GLYCOSYLATION, TISSUE SPECIFICITY, COFACTOR, SUBUNIT.
    Strain: Wistar.
    Tissue: Seminal vesicle.
  2. "Sulfhydryl oxidase (SOx) from mouse epidermis: molecular cloning, nucleotide sequence, and expression of recombinant protein in the cultured cells."
    Matsuba S., Suga Y., Ishidoh K., Hashimoto Y., Takamori K., Kominami E., Wilhelm B., Seitz J., Ogawa H.
    J. Dermatol. Sci. 30:50-62(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), PROTEIN SEQUENCE OF 33-65, IDENTIFICATION BY MASS SPECTROMETRY.
  3. "Identification and expression of a new splicing variant of FAD-sulfhydryl oxidase in adult rat brain."
    Radom J., Colin D., Thiebault F., Dognin-Bergeret M.J., Mairet-Coello G., Esnard-Feve A., Fellmann D., Jouvenot M.
    Biochim. Biophys. Acta 1759:225-233(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Wistar.
    Tissue: Brain.

Entry informationi

Entry nameiQSOX1_RAT
AccessioniPrimary (citable) accession number: Q6IUU3
Secondary accession number(s): Q8K4M2, Q99J80
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: July 5, 2004
Last modified: May 14, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi