Sulfhydryl oxidase 1 precursor - Rattus norvegicus (Rat)

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Q6IUU3 (QSOX1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 65. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Sulfhydryl oxidase 1
Short name=rQSOX
Short name=rSOx
EC=1.8.3.2

Alternative name(s):
FAD-dependent sulfhydryl oxidase-2
Short name=SOx-2
Quiescin Q6

Gene names

Name:	Qsox1
Synonyms:	Qscn6, Sox2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	750 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the oxidation of sulfhydryl groups in peptide and protein thiols to disulfides with the reduction of oxygen to hydrogen peroxide. May contribute to disulfide bond formation in a variety of secreted proteins. Ref.3
Catalytic activity	2 R'C(R)SH + O₂ = R'C(R)S-S(R)CR' + H₂O₂.
Cofactor	Binds 1 FAD per subunit. Ref.1
Subunit structure	Monomer. Ref.1
Subcellular location	Isoform 1: Golgi apparatus membrane; Single-pass membrane protein By similarity. Isoform 2: Secreted By similarity. Note: Found in the extracellular medium of quiescent cells but is not found in proliferating cells By similarity.
Tissue specificity	Isoform 1 is expressed in brain, hypophysis, heart, testis and the seminal vesicle. Isoform 3 is highly expressed in the seminal vesicles followed by testis, heart, brain, thymus, hypophysis and lung. Also expressed in prostate, kidney, spleen, liver. Ref.1 Ref.3
Sequence similarities	Belongs to the quiescin-sulfhydryl oxidase (QSOX) family. Contains 1 ERV/ALR sulfhydryl oxidase domain. Contains 1 thioredoxin domain.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane Secreted
Coding sequence diversity	Alternative splicing
Domain	Signal Transmembrane Transmembrane helix
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro protein thiol-disulfide exchange Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	extracellular space Inferred from sequence or structural similarity. Source: UniProtKB integral to Golgi membrane Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	flavin-linked sulfhydryl oxidase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q6IUU3-1) Also known as: QSOX-L; This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q6IUU3-2) The sequence of this isoform differs from the canonical sequence as follows: 27-32: Missing. 569-570: VM → LL 571-750: Missing.
Note: No experimental confirmation available.

Isoform 3 (identifier: Q6IUU3-3) The sequence of this isoform differs from the canonical sequence as follows: 569-570: VM → LL 571-750: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 32

Ref.2

Chain

33 – 750

718

Sulfhydryl oxidase 1

PRO_0000249535

Regions

Transmembrane

710 – 730

Helical; Potential

Domain

33 – 159

127

Thioredoxin

Domain

399 – 506

108

ERV/ALR sulfhydryl oxidase

Nucleotide binding

481 – 488

FAD By similarity

Sites

Active site

Nucleophile By similarity

Active site

Nucleophile By similarity

Binding site

404

FAD By similarity

Binding site

411

FAD By similarity

Binding site

415

FAD By similarity

Binding site

454

FAD By similarity

Binding site

458

FAD By similarity

Binding site

503

FAD By similarity

Binding site

506

FAD By similarity

Amino acid modifications

Glycosylation

133

N-linked (GlcNAc...) Potential

Glycosylation

246

N-linked (GlcNAc...) Potential

Disulfide bond

73 ↔ 76

Redox-active By similarity

Disulfide bond

396 ↔ 408

By similarity

Disulfide bond

452 ↔ 455

By similarity

Disulfide bond

512 ↔ 515

By similarity

Natural variations

Alternative sequence

27 – 32

Missing in isoform 2.

VSP_020499

Alternative sequence

569 – 570

VM → LL in isoform 2 and isoform 3.

VSP_020497

Alternative sequence

571 – 750

180

Missing in isoform 2 and isoform 3.

VSP_020498

Experimental info

Sequence conflict

352

N → S in AAM67412. Ref.1

Sequences

Sequence		Length	Mass (Da)
Isoform 1 (QSOX-L) [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 1781E657ED085114 Show »	FASTA	750	82,412
10 20 30 40 50 60 MRRCGRHSGP PSLLLLLLLL PPLLLSVPGA YAARLSVLYS SSDPLTLLDA DTVRPAVLGS 70 80 90 100 110 120 SSAWAVEFFA SWCGHCIAFA PTWKELANDV KDWRPALNLA VLDCADETNS AVCREFNIAG 130 140 150 160 170 180 FPTVRFFKAF SKNGTGTALP AAGANVQTLR MRLIDALESH RDTWPPACPP LEPAKLKDIN 190 200 210 220 230 240 EFFTRSKAEY LALIFEREDS YLGREVTLDL SQFHAVAVRR VLNSESDVVS KFAVTDFPSC 250 260 270 280 290 300 YLLLRNGSVS RVPVLVESRP FYTSYLRGLP GLTREAPPTT AAPVTPDKIA PTVWKFADRS 310 320 330 340 350 360 KIYMADLESA LHYILRVEVG KFSVLEGQRL VALKKFVAVL AKYFPGQPLV QNFLHSINDW 370 380 390 400 410 420 LQKQQKKKIP YSYFKAALDS RKENAVLAEK VNWIGCQGSE PHFRGFPCSL WVLFHFLTVQ 430 440 450 460 470 480 AHRYSEAHPQ EPADGQEVLQ AMRSYVQSFF GCRDCANHFE QMAAASMHQV KSPSNAVLWL 490 500 510 520 530 540 WTSHNRVNAR LSGALSEDPQ FPKVQWPPRE LCSACHNEVN GQVPLWDLGA TLNFLKAHFS 550 560 570 580 590 600 PANIVRDPPA PGPASRRGTQ DPEASPNLVM DTLKLETGNS VLGHEQAASA ASPGATALDV 610 620 630 640 650 660 PAGKPEASGP QELNAGLSMG GASPGQGPPE HTEELLRDVQ ENAQGQQHLS KRDTEALLLP 670 680 690 700 710 720 EVNHLQGPLA PRRGGHSPKQ LASILEGEPE ALAIQGRRQW LQVLGGGVSF LDISLCVGLY 730 740 750 SVSFMGLLAM YTYFRARMRT PKGHVSYPTA « Hide
Isoform 2 [UniParc]. Checksum: B2E7B42708BD3B59 Show »	FASTA	564	62,883
10 20 30 40 50 60 MRRCGRHSGP PSLLLLLLLL PPLLLSARLS VLYSSSDPLT LLDADTVRPA VLGSSSAWAV 70 80 90 100 110 120 EFFASWCGHC IAFAPTWKEL ANDVKDWRPA LNLAVLDCAD ETNSAVCREF NIAGFPTVRF 130 140 150 160 170 180 FKAFSKNGTG TALPAAGANV QTLRMRLIDA LESHRDTWPP ACPPLEPAKL KDINEFFTRS 190 200 210 220 230 240 KAEYLALIFE REDSYLGREV TLDLSQFHAV AVRRVLNSES DVVSKFAVTD FPSCYLLLRN 250 260 270 280 290 300 GSVSRVPVLV ESRPFYTSYL RGLPGLTREA PPTTAAPVTP DKIAPTVWKF ADRSKIYMAD 310 320 330 340 350 360 LESALHYILR VEVGKFSVLE GQRLVALKKF VAVLAKYFPG QPLVQNFLHS INDWLQKQQK 370 380 390 400 410 420 KKIPYSYFKA ALDSRKENAV LAEKVNWIGC QGSEPHFRGF PCSLWVLFHF LTVQAHRYSE 430 440 450 460 470 480 AHPQEPADGQ EVLQAMRSYV QSFFGCRDCA NHFEQMAAAS MHQVKSPSNA VLWLWTSHNR 490 500 510 520 530 540 VNARLSGALS EDPQFPKVQW PPRELCSACH NEVNGQVPLW DLGATLNFLK AHFSPANIVR 550 560 DPPAPGPASR RGTQDPEASP NLLL « Hide
Isoform 3 [UniParc]. Checksum: FF53F8D2D1C7F4A9 Show »	FASTA	570	63,442
10 20 30 40 50 60 MRRCGRHSGP PSLLLLLLLL PPLLLSVPGA YAARLSVLYS SSDPLTLLDA DTVRPAVLGS 70 80 90 100 110 120 SSAWAVEFFA SWCGHCIAFA PTWKELANDV KDWRPALNLA VLDCADETNS AVCREFNIAG 130 140 150 160 170 180 FPTVRFFKAF SKNGTGTALP AAGANVQTLR MRLIDALESH RDTWPPACPP LEPAKLKDIN 190 200 210 220 230 240 EFFTRSKAEY LALIFEREDS YLGREVTLDL SQFHAVAVRR VLNSESDVVS KFAVTDFPSC 250 260 270 280 290 300 YLLLRNGSVS RVPVLVESRP FYTSYLRGLP GLTREAPPTT AAPVTPDKIA PTVWKFADRS 310 320 330 340 350 360 KIYMADLESA LHYILRVEVG KFSVLEGQRL VALKKFVAVL AKYFPGQPLV QNFLHSINDW 370 380 390 400 410 420 LQKQQKKKIP YSYFKAALDS RKENAVLAEK VNWIGCQGSE PHFRGFPCSL WVLFHFLTVQ 430 440 450 460 470 480 AHRYSEAHPQ EPADGQEVLQ AMRSYVQSFF GCRDCANHFE QMAAASMHQV KSPSNAVLWL 490 500 510 520 530 540 WTSHNRVNAR LSGALSEDPQ FPKVQWPPRE LCSACHNEVN GQVPLWDLGA TLNFLKAHFS 550 560 570 PANIVRDPPA PGPASRRGTQ DPEASPNLLL « Hide

References

[1]	"Rat seminal vesicle FAD-dependent sulfhydryl oxidase: biochemical characterization and molecular cloning of a member of the new sulfhydryl oxidase/quiescin Q6 gene family." Benayoun B., Esnard-Feve A., Castella S., Courty Y., Esnard F. J. Biol. Chem. 276:13830-13837(2001) [PubMed: 11278790] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2 AND 3), GLYCOSYLATION, TISSUE SPECIFICITY, COFACTOR, SUBUNIT. Strain: Wistar. Tissue: Seminal vesicle.
[2]	"Sulfhydryl oxidase (SOx) from mouse epidermis: molecular cloning, nucleotide sequence, and expression of recombinant protein in the cultured cells." Matsuba S., Suga Y., Ishidoh K., Hashimoto Y., Takamori K., Kominami E., Wilhelm B., Seitz J., Ogawa H. J. Dermatol. Sci. 30:50-62(2002) [PubMed: 12354420] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), PROTEIN SEQUENCE OF 33-65, MASS SPECTROMETRY.
[3]	"Identification and expression of a new splicing variant of FAD-sulfhydryl oxidase in adult rat brain." Radom J., Colin D., Thiebault F., Dognin-Bergeret M.J., Mairet-Coello G., Esnard-Feve A., Fellmann D., Jouvenot M. Biochim. Biophys. Acta 1759:225-233(2006) [PubMed: 16806532] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, MASS SPECTROMETRY. Strain: Wistar. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF217799 mRNA. Translation: AAM67412.1. AF285078 mRNA. Translation: AAG53892.1. AB044285 mRNA. Translation: BAB21937.1. AY623665 mRNA. Translation: AAT40988.1.
IPI	IPI00200685. IPI00782633. IPI00786837.
RefSeq	NP_001103368.1. NM_001109898.1. NP_445883.1. NM_053431.3.
UniGene	Rn.44920.
3D structure databases
HSSP	HSSP built from PDB template 1X5D based on UniProtKB Q15084.
ProteinModelPortal	Q6IUU3.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6IUU3.
Proteomic databases
PRIDE	Q6IUU3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000068044; ENSRNOP00000061072; ENSRNOG00000003649.
GeneID	84491.
KEGG	rno:84491.
UCSC	AY623665. rat.
Organism-specific databases
CTD	5768.
RGD	68957. Qsox1.
Phylogenomic databases
eggNOG	roNOG14722.
GeneTree	ENSGT00390000008045.
HOVERGEN	HBG080360.
InParanoid	Q6IUU3.
OMA	SHNRVNA.
OrthoDB	EOG4JT05B.
Enzyme and pathway databases
BRENDA	1.8.3.2. 5301.
Gene expression databases
ArrayExpress	Q6IUU3.
Genevestigator	Q6IUU3.
GermOnline	ENSRNOG00000003649. Rattus norvegicus.
Family and domain databases
InterPro	IPR017905. ERV/ALR_sulphydryl_oxidase. IPR006863. Evr1_Alr. IPR012336. Thioredoxin-like_fold. IPR013766. Thioredoxin_domain. [Graphical view]
Gene3D	G3DSA:1.20.120.310. Evr1_Alr. 1 hit. G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
KO	K10758.
Pfam	PF04777. Evr1_Alr. 1 hit. PF00085. Thioredoxin. 1 hit. [Graphical view]
SUPFAM	SSF69000. Evr1_Alr. 1 hit. SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS51324. ERV_ALR. 1 hit. PS51352. THIOREDOXIN_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	617042.

Entry information

Entry name

QSOX1_RAT

Accession

Primary (citable) accession number: Q6IUU3
Secondary accession number(s): Q8K4M2, Q99J80

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2006
Last sequence update:	July 5, 2004
Last modified:	November 16, 2011
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Natural variations

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents