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Q6IUU3 (QSOX1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sulfhydryl oxidase 1

Short name=rQSOX
Short name=rSOx
EC=1.8.3.2
Alternative name(s):
FAD-dependent sulfhydryl oxidase-2
Short name=SOx-2
Quiescin Q6
Gene names
Name:Qsox1
Synonyms:Qscn6, Sox2
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length750 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the oxidation of sulfhydryl groups in peptide and protein thiols to disulfides with the reduction of oxygen to hydrogen peroxide. May contribute to disulfide bond formation in a variety of secreted proteins. Ref.3

Catalytic activity

2 R'C(R)SH + O2 = R'C(R)S-S(R)CR' + H2O2.

Cofactor

Binds 1 FAD per subunit. Ref.1

Subunit structure

Monomer. Ref.1

Subcellular location

Isoform 1: Golgi apparatus membrane; Single-pass membrane protein By similarity.

Isoform 2: Secreted By similarity. Note: Found in the extracellular medium of quiescent cells but is not found in proliferating cells By similarity.

Tissue specificity

Isoform 1 is expressed in brain, hypophysis, heart, testis and the seminal vesicle. Isoform 3 is highly expressed in the seminal vesicles followed by testis, heart, brain, thymus, hypophysis and lung. Also expressed in prostate, kidney, spleen, liver. Ref.1 Ref.3

Sequence similarities

Belongs to the quiescin-sulfhydryl oxidase (QSOX) family.

Contains 1 ERV/ALR sulfhydryl oxidase domain.

Contains 1 thioredoxin domain.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q6IUU3-1)

Also known as: QSOX-L;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q6IUU3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     27-32: Missing.
     569-570: VM → LL
     571-750: Missing.
Note: No experimental confirmation available.
Isoform 3 (identifier: Q6IUU3-3)

The sequence of this isoform differs from the canonical sequence as follows:
     569-570: VM → LL
     571-750: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3232 Ref.2
Chain33 – 750718Sulfhydryl oxidase 1
PRO_0000249535

Regions

Transmembrane710 – 73021Helical; Potential
Domain33 – 159127Thioredoxin
Domain399 – 506108ERV/ALR sulfhydryl oxidase
Nucleotide binding481 – 4888FAD By similarity

Sites

Active site731Nucleophile By similarity
Active site761Nucleophile By similarity
Binding site4041FAD By similarity
Binding site4111FAD By similarity
Binding site4151FAD By similarity
Binding site4541FAD By similarity
Binding site4581FAD By similarity
Binding site5031FAD By similarity
Binding site5061FAD By similarity

Amino acid modifications

Glycosylation1331N-linked (GlcNAc...) Potential
Glycosylation2461N-linked (GlcNAc...) Potential
Disulfide bond73 ↔ 76Redox-active By similarity
Disulfide bond396 ↔ 408 By similarity
Disulfide bond452 ↔ 455 By similarity
Disulfide bond512 ↔ 515 By similarity

Natural variations

Alternative sequence27 – 326Missing in isoform 2.
VSP_020499
Alternative sequence569 – 5702VM → LL in isoform 2 and isoform 3.
VSP_020497
Alternative sequence571 – 750180Missing in isoform 2 and isoform 3.
VSP_020498

Experimental info

Sequence conflict3521N → S in AAM67412. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (QSOX-L) [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 1781E657ED085114

FASTA75082,412
        10         20         30         40         50         60 
MRRCGRHSGP PSLLLLLLLL PPLLLSVPGA YAARLSVLYS SSDPLTLLDA DTVRPAVLGS 

        70         80         90        100        110        120 
SSAWAVEFFA SWCGHCIAFA PTWKELANDV KDWRPALNLA VLDCADETNS AVCREFNIAG 

       130        140        150        160        170        180 
FPTVRFFKAF SKNGTGTALP AAGANVQTLR MRLIDALESH RDTWPPACPP LEPAKLKDIN 

       190        200        210        220        230        240 
EFFTRSKAEY LALIFEREDS YLGREVTLDL SQFHAVAVRR VLNSESDVVS KFAVTDFPSC 

       250        260        270        280        290        300 
YLLLRNGSVS RVPVLVESRP FYTSYLRGLP GLTREAPPTT AAPVTPDKIA PTVWKFADRS 

       310        320        330        340        350        360 
KIYMADLESA LHYILRVEVG KFSVLEGQRL VALKKFVAVL AKYFPGQPLV QNFLHSINDW 

       370        380        390        400        410        420 
LQKQQKKKIP YSYFKAALDS RKENAVLAEK VNWIGCQGSE PHFRGFPCSL WVLFHFLTVQ 

       430        440        450        460        470        480 
AHRYSEAHPQ EPADGQEVLQ AMRSYVQSFF GCRDCANHFE QMAAASMHQV KSPSNAVLWL 

       490        500        510        520        530        540 
WTSHNRVNAR LSGALSEDPQ FPKVQWPPRE LCSACHNEVN GQVPLWDLGA TLNFLKAHFS 

       550        560        570        580        590        600 
PANIVRDPPA PGPASRRGTQ DPEASPNLVM DTLKLETGNS VLGHEQAASA ASPGATALDV 

       610        620        630        640        650        660 
PAGKPEASGP QELNAGLSMG GASPGQGPPE HTEELLRDVQ ENAQGQQHLS KRDTEALLLP 

       670        680        690        700        710        720 
EVNHLQGPLA PRRGGHSPKQ LASILEGEPE ALAIQGRRQW LQVLGGGVSF LDISLCVGLY 

       730        740        750 
SVSFMGLLAM YTYFRARMRT PKGHVSYPTA 

« Hide

Isoform 2 [UniParc].

Checksum: B2E7B42708BD3B59
Show »

FASTA56462,883
Isoform 3 [UniParc].

Checksum: FF53F8D2D1C7F4A9
Show »

FASTA57063,442

References

[1]"Rat seminal vesicle FAD-dependent sulfhydryl oxidase: biochemical characterization and molecular cloning of a member of the new sulfhydryl oxidase/quiescin Q6 gene family."
Benayoun B., Esnard-Feve A., Castella S., Courty Y., Esnard F.
J. Biol. Chem. 276:13830-13837(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2 AND 3), GLYCOSYLATION, TISSUE SPECIFICITY, COFACTOR, SUBUNIT.
Strain: Wistar.
Tissue: Seminal vesicle.
[2]"Sulfhydryl oxidase (SOx) from mouse epidermis: molecular cloning, nucleotide sequence, and expression of recombinant protein in the cultured cells."
Matsuba S., Suga Y., Ishidoh K., Hashimoto Y., Takamori K., Kominami E., Wilhelm B., Seitz J., Ogawa H.
J. Dermatol. Sci. 30:50-62(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), PROTEIN SEQUENCE OF 33-65, IDENTIFICATION BY MASS SPECTROMETRY.
[3]"Identification and expression of a new splicing variant of FAD-sulfhydryl oxidase in adult rat brain."
Radom J., Colin D., Thiebault F., Dognin-Bergeret M.J., Mairet-Coello G., Esnard-Feve A., Fellmann D., Jouvenot M.
Biochim. Biophys. Acta 1759:225-233(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: Wistar.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF217799 mRNA. Translation: AAM67412.1.
AF285078 mRNA. Translation: AAG53892.1.
AB044285 mRNA. Translation: BAB21937.1.
AY623665 mRNA. Translation: AAT40988.1.
RefSeqNP_001103368.1. NM_001109898.1. [Q6IUU3-1]
NP_445883.1. NM_053431.3. [Q6IUU3-3]
UniGeneRn.44920.

3D structure databases

ProteinModelPortalQ6IUU3.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PaxDbQ6IUU3.
PRIDEQ6IUU3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000005052; ENSRNOP00000005052; ENSRNOG00000003649. [Q6IUU3-3]
ENSRNOT00000068044; ENSRNOP00000061072; ENSRNOG00000003649. [Q6IUU3-2]
GeneID84491.
KEGGrno:84491.
UCSCRGD:68957. rat. [Q6IUU3-1]

Organism-specific databases

CTD5768.
RGD68957. Qsox1.

Phylogenomic databases

eggNOGCOG0526.
GeneTreeENSGT00390000008045.
HOGENOMHOG000231631.
HOVERGENHBG080360.
InParanoidQ6IUU3.
KOK10758.
OMASHNRVNA.
OrthoDBEOG73RB9X.
PhylomeDBQ6IUU3.

Enzyme and pathway databases

BRENDA1.8.3.2. 5301.

Gene expression databases

GenevestigatorQ6IUU3.

Family and domain databases

Gene3D1.20.120.310. 1 hit.
3.40.30.10. 1 hit.
InterProIPR017905. ERV/ALR_sulphydryl_oxidase.
IPR012336. Thioredoxin-like_fold.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamPF04777. Evr1_Alr. 1 hit.
PF00085. Thioredoxin. 1 hit.
[Graphical view]
SUPFAMSSF52833. SSF52833. 1 hit.
SSF69000. SSF69000. 1 hit.
PROSITEPS51324. ERV_ALR. 1 hit.
PS51352. THIOREDOXIN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio617042.
PROQ6IUU3.

Entry information

Entry nameQSOX1_RAT
AccessionPrimary (citable) accession number: Q6IUU3
Secondary accession number(s): Q8K4M2, Q99J80
Entry history
Integrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: July 5, 2004
Last modified: May 14, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families