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Protein

Spermatogenesis- and oogenesis-specific basic helix-loop-helix-containing protein 1

Gene

Sohlh1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription regulator of both male and female germline differentiation. Suppresses genes involved in spermatogonial stem cells maintenance, and induces genes important for spermatogonial differentiation (PubMed:22056784). Coordinates oocyte differentiation without affecting meiosis I (PubMed:28504655).2 Publications

GO - Molecular functioni

  • core promoter sequence-specific DNA binding Source: GO_Central
  • DNA binding transcription factor activity Source: MGI
  • protein heterodimerization activity Source: UniProtKB
  • protein homodimerization activity Source: UniProtKB
  • RNA polymerase II regulatory region sequence-specific DNA binding Source: NTNU_SB
  • transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: NTNU_SB

GO - Biological processi

  • cell differentiation Source: UniProtKB
  • oocyte differentiation Source: UniProtKB
  • oogenesis Source: MGI
  • ovarian follicle development Source: MGI
  • positive regulation of transcription, DNA-templated Source: MGI
  • positive regulation of transcription by RNA polymerase II Source: NTNU_SB
  • regulation of gene expression Source: MGI
  • spermatogenesis Source: UniProtKB

Keywordsi

Molecular functionDevelopmental protein, DNA-binding
Biological processDifferentiation, Spermatogenesis, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Spermatogenesis- and oogenesis-specific basic helix-loop-helix-containing protein 1
Gene namesi
Name:Sohlh1
Synonyms:Gm110, Tohlh1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:2684956 Sohlh1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Knockout mice are infertile. Males lacking SOHLH1 display disrupted spermatogonial differentiation into spermatocytes and show a strong down-regulation Lhx8 and Neurog3/Ngn3 genes (PubMed:16564520). Females display perturbed follicular formation, probably partially due to down-regulation of Nobox and Figla, 2 genes required for folliculogenesis (PubMed:16690745). Fertility could be restored by conditional expression of a SOHLH1 transgene after the onset of meiosis (PubMed:28504655).3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003156991 – 357Spermatogenesis- and oogenesis-specific basic helix-loop-helix-containing protein 1Add BLAST357

Proteomic databases

PaxDbiQ6IUP1
PRIDEiQ6IUP1

PTM databases

PhosphoSitePlusiQ6IUP1

Expressioni

Tissue specificityi

In males, it is mainly expressed in testis, while in females it is mainly expressed in ovary. In testis, it is exclusively expressed in spermatogonia, with a preference for prespermatogonia and type A spermatogonia. In ovary, it is detected in germ cell cysts, primordial follicles, and primary follicles but is undetectable by the secondary follicle stage (at protein level). Expressed in the majority of spermatogonia in adult animals, but not in the most undifferentiated spermatogonial population (PubMed:22056784).3 Publications

Developmental stagei

In male testis, it is expressed as early as E12.5. After birth, it localizes to type A spermatogonia in 7-day-old testis and adult testis, but not in spermatocytes. In spermatogonia, it is initially detected in stage IV Aal spermatogonia and strongly expressed in Aal, A1, A2, A3, A4, intermediate and type B spermatogonia (at protein level). In ovary, it is detected at E15.5, when oocytes have entered meiosis I, although a low level expression is detectable at E13.5. Expressed in oocytes of germ cell cysts as well as primordial follicles in the newborn ovary. In adult ovaries, it is preferentially expressed in primordial oocytes but disappear rapidly as the oocytes are recruited to form primary and secondary (multilayer and preantral) follicles. During oocyte differentiation, protein appearance at E15.5 correlates with SOHLH2 translocation from the cytoplasm into the nucleus and is dependent on SOHLH2 expression (PubMed:28504655).3 Publications

Inductioni

Transcription is activated by DMRT1 in undifferentiated spermatogonia.1 Publication

Gene expression databases

BgeeiENSMUSG00000059625
GenevisibleiQ6IUP1 MM

Interactioni

Subunit structurei

Forms both hetero- and homodimers with SOHLH2.1 Publication

GO - Molecular functioni

  • protein heterodimerization activity Source: UniProtKB
  • protein homodimerization activity Source: UniProtKB

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000076253

Structurei

3D structure databases

ProteinModelPortaliQ6IUP1
SMRiQ6IUP1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini54 – 105bHLHPROSITE-ProRule annotationAdd BLAST52

Phylogenomic databases

eggNOGiENOG410J1JP Eukaryota
ENOG4111AXY LUCA
GeneTreeiENSGT00390000000656
HOGENOMiHOG000060205
InParanoidiQ6IUP1
KOiK22495
OMAiGALSCCE
OrthoDBiEOG091G0V6C
PhylomeDBiQ6IUP1
TreeFamiTF336841

Family and domain databases

Gene3Di4.10.280.10, 1 hit
InterProiView protein in InterPro
IPR011598 bHLH_dom
IPR036638 HLH_DNA-bd_sf
IPR032668 SOHLH1
PANTHERiPTHR16223:SF14 PTHR16223:SF14, 1 hit
PfamiView protein in Pfam
PF00010 HLH, 1 hit
SUPFAMiSSF47459 SSF47459, 1 hit
PROSITEiView protein in PROSITE
PS50888 BHLH, 1 hit

Sequencei

Sequence statusi: Complete.

Q6IUP1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASGGHERAN EDYRVSGITG CSKTPQPETQ DSLQTSSQSS ALCTAPVAAA
60 70 80 90 100
NLGPSLRRNV VSERERRRRI SLSCEHLRAL LPQFDGRRED MASVLEMSVY
110 120 130 140 150
FLQLAHSMDP SWEQLSVPQP PQEMWHMWQG DVLQVTLANQ IADSKPDSGI
160 170 180 190 200
AKPSAVSRVQ DPPCFGMLDT DQSQATERES ELLERPSSCP GHRQSALSFS
210 220 230 240 250
EPESSSLGPG LPPWIPHSWQ PATPEASDIV PGGSHQVASL AGDPESSGML
260 270 280 290 300
AEEANLVLAS VPDARYTTGA GSDVVDGAPF LMTTNPDWWL GSVEGRGGPA
310 320 330 340 350
LARSSPVDGA EPSFIGDPEL CSQELQAGPG ELWGLDFGSP GLALKDEADS

IFPDFFP
Length:357
Mass (Da):38,188
Last modified:July 5, 2004 - v1
Checksum:i09F71E4D591997CA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY623913 mRNA Translation: AAT39886.1
AL731682 Genomic DNA No translation available.
BC139189 mRNA Translation: AAI39190.1
BC139190 mRNA Translation: AAI39191.1
CCDSiCCDS15793.1
RefSeqiNP_001001714.1, NM_001001714.1
UniGeneiMm.301632

Genome annotation databases

EnsembliENSMUST00000076989; ENSMUSP00000076253; ENSMUSG00000059625
GeneIDi227631
KEGGimmu:227631
UCSCiuc008itn.1 mouse

Similar proteinsi

Entry informationi

Entry nameiSOLH1_MOUSE
AccessioniPrimary (citable) accession number: Q6IUP1
Secondary accession number(s): B2RTA1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 5, 2004
Last modified: May 23, 2018
This is version 99 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

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