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Protein

Spermatogenesis- and oogenesis-specific basic helix-loop-helix-containing protein 1

Gene

Sohlh1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor expressed in undifferentiated spermatogonia required for spermatogonial development.2 Publications

GO - Molecular functioni

GO - Biological processi

  • cell differentiation Source: MGI
  • oogenesis Source: MGI
  • ovarian follicle development Source: MGI
  • positive regulation of transcription, DNA-templated Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: NTNU_SB
  • regulation of gene expression Source: MGI
  • spermatogenesis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Spermatogenesis, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Spermatogenesis- and oogenesis-specific basic helix-loop-helix-containing protein 1
Gene namesi
Name:Sohlh1
Synonyms:Gm110, Tohlh1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:2684956. Sohlh1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice are infertile. Males lacking Sohlh1 display disrupted spermatogonial differentiation into spermatocytes and show a strong down-regulation Lhx8 and Neurog3/Ngn3 genes. Females display perturbed follicular formation, probably partially due to down-regulation of Nobox and Figla, 2 genes required for folliculogenesis.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 357357Spermatogenesis- and oogenesis-specific basic helix-loop-helix-containing protein 1PRO_0000315699Add
BLAST

Proteomic databases

PaxDbiQ6IUP1.
PRIDEiQ6IUP1.

Expressioni

Tissue specificityi

In males, it is mainly expressed in testis, while in females it is mainly expressed in ovary. In testis, it is exclusively expressed in spermatogonia, with a preference for prespermatogonia and type A spermatogonia. In ovary, it is detected in germ cell cysts, primordial follicles, and primary follicles but is undetectable by the secondary follicle stage (at protein level).2 Publications

Developmental stagei

In male testis, it is expressed as early as E12.5. After birth, it localizes to type A spermatogonia in 7-day-old testis and adult testis, but not in spermatocytes. In spermatogonia, it is initially detected in stage IV Aal spermatogonia and strongly expressed in Aal, A1, A2, A3, A4, intermediate and type B spermatogonia (at protein level). In ovary, it is detected at E15.5, when oocytes have entered meiosis I, although a low level expression is detectable at E13.5. Expressed in oocytes of germ cell cysts as well as primordial follicles in the newborn ovary. In adult ovaries, it is preferentially expressed in primordial oocytes but disappear rapidly as the oocytes are recruited to form primary and secondary (multilayer and preantral) follicles.2 Publications

Inductioni

Transcription is activated by DMRT1 in undifferentiated spermatogonia.1 Publication

Gene expression databases

BgeeiQ6IUP1.
GenevisibleiQ6IUP1. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000076253.

Structurei

3D structure databases

ProteinModelPortaliQ6IUP1.
SMRiQ6IUP1. Positions 58-104.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini54 – 10552bHLHPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410J1JP. Eukaryota.
ENOG4111AXY. LUCA.
GeneTreeiENSGT00390000000656.
HOGENOMiHOG000060205.
InParanoidiQ6IUP1.
OMAiPTVRGCN.
OrthoDBiEOG7B31P6.
PhylomeDBiQ6IUP1.
TreeFamiTF336841.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
IPR032668. SOHLH1.
[Graphical view]
PANTHERiPTHR16223:SF14. PTHR16223:SF14. 1 hit.
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6IUP1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASGGHERAN EDYRVSGITG CSKTPQPETQ DSLQTSSQSS ALCTAPVAAA
60 70 80 90 100
NLGPSLRRNV VSERERRRRI SLSCEHLRAL LPQFDGRRED MASVLEMSVY
110 120 130 140 150
FLQLAHSMDP SWEQLSVPQP PQEMWHMWQG DVLQVTLANQ IADSKPDSGI
160 170 180 190 200
AKPSAVSRVQ DPPCFGMLDT DQSQATERES ELLERPSSCP GHRQSALSFS
210 220 230 240 250
EPESSSLGPG LPPWIPHSWQ PATPEASDIV PGGSHQVASL AGDPESSGML
260 270 280 290 300
AEEANLVLAS VPDARYTTGA GSDVVDGAPF LMTTNPDWWL GSVEGRGGPA
310 320 330 340 350
LARSSPVDGA EPSFIGDPEL CSQELQAGPG ELWGLDFGSP GLALKDEADS

IFPDFFP
Length:357
Mass (Da):38,188
Last modified:July 5, 2004 - v1
Checksum:i09F71E4D591997CA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY623913 mRNA. Translation: AAT39886.1.
AL731682 Genomic DNA. Translation: CAM14271.1.
BC139189 mRNA. Translation: AAI39190.1.
BC139190 mRNA. Translation: AAI39191.1.
CCDSiCCDS15793.1.
RefSeqiNP_001001714.1. NM_001001714.1.
UniGeneiMm.301632.

Genome annotation databases

EnsembliENSMUST00000076989; ENSMUSP00000076253; ENSMUSG00000059625.
GeneIDi227631.
KEGGimmu:227631.
UCSCiuc008itn.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY623913 mRNA. Translation: AAT39886.1.
AL731682 Genomic DNA. Translation: CAM14271.1.
BC139189 mRNA. Translation: AAI39190.1.
BC139190 mRNA. Translation: AAI39191.1.
CCDSiCCDS15793.1.
RefSeqiNP_001001714.1. NM_001001714.1.
UniGeneiMm.301632.

3D structure databases

ProteinModelPortaliQ6IUP1.
SMRiQ6IUP1. Positions 58-104.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000076253.

Proteomic databases

PaxDbiQ6IUP1.
PRIDEiQ6IUP1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000076989; ENSMUSP00000076253; ENSMUSG00000059625.
GeneIDi227631.
KEGGimmu:227631.
UCSCiuc008itn.1. mouse.

Organism-specific databases

CTDi402381.
MGIiMGI:2684956. Sohlh1.

Phylogenomic databases

eggNOGiENOG410J1JP. Eukaryota.
ENOG4111AXY. LUCA.
GeneTreeiENSGT00390000000656.
HOGENOMiHOG000060205.
InParanoidiQ6IUP1.
OMAiPTVRGCN.
OrthoDBiEOG7B31P6.
PhylomeDBiQ6IUP1.
TreeFamiTF336841.

Miscellaneous databases

PROiQ6IUP1.
SOURCEiSearch...

Gene expression databases

BgeeiQ6IUP1.
GenevisibleiQ6IUP1. MM.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
IPR032668. SOHLH1.
[Graphical view]
PANTHERiPTHR16223:SF14. PTHR16223:SF14. 1 hit.
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Oogenesis requires germ cell-specific transcriptional regulators Sohlh1 and Lhx8."
    Pangas S.A., Choi Y., Ballow D.J., Zhao Y., Westphal H., Matzuk M.M., Rajkovic A.
    Proc. Natl. Acad. Sci. U.S.A. 103:8090-8095(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
    Strain: C57BL/6J.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  4. "Sohlh1 is essential for spermatogonial differentiation."
    Ballow D., Meistrich M.L., Matzuk M., Rajkovic A.
    Dev. Biol. 294:161-167(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
  5. "The mammalian doublesex homolog DMRT1 is a transcriptional gatekeeper that controls the mitosis versus meiosis decision in male germ cells."
    Matson C.K., Murphy M.W., Griswold M.D., Yoshida S., Bardwell V.J., Zarkower D.
    Dev. Cell 19:612-624(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.

Entry informationi

Entry nameiSOLH1_MOUSE
AccessioniPrimary (citable) accession number: Q6IUP1
Secondary accession number(s): B2RTA1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 5, 2004
Last modified: July 6, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.