Q6IUF8 (L_MACHU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA-directed RNA polymerase L
Short name=Protein L
EC=2.7.7.48

Alternative name(s):
Large structural protein
Replicase
Transcriptase

Gene names

Name:

L

Organism

Machupo virus (MACV) [Complete proteome]

Taxonomic identifier

11628 [NCBI]

Taxonomic lineage

Viruses › ssRNA negative-strand viruses › Arenaviridae › Arenavirus › New world arenaviruses

Virus host

Chlorocebus aethiops (Green monkey) (Cercopithecus aethiops) [TaxID: 9534]
Homo sapiens (Human) [TaxID: 9606]
Calomys callosus (Large vesper mouse) [TaxID: 56210]

Protein attributes

Sequence length	2209 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	RNA-dependent RNA polymerase which is responsible for replication and transcription of the viral RNA genome. During transcription, synthesizes 4 subgenomic RNAs, and assures their capping by a cap-snatching mechanism, in which cellular capped pre-mRNA are used to generate primers for viral transcription. The 3'-end of subgenomic mRNAs molecules are heterogeneous and not polyadenylated. The replicase function is to direct synthesis of antigenomic and genomic RNA which are encapsidated and non capped. As a consequence of the use of the same enzyme for both transcription and replication, these mechanisms need to be well coordinated. These processes may be regulated by proteins N and Z in a dose-dependent manner By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	Homomultimerizes; the oligomeric structure is essential for the polymerase activity. Interacts with the nucleocapsid protein N By similarity. Interacts with protein Z; this interaction inhibits viral transcription and replication By similarity.
Subcellular location	Virion. Host cytoplasm.
Sequence similarities	Belongs to the arenaviridae RNA polymerase family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	Cap snatching RNA replication
Cellular component	Host cytoplasm Virion
Ligand	Nucleotide-binding
Molecular function	Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	RNA biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW viral genome replication Inferred from electronic annotation. Source: InterPro
Cellular component	host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2209	2209	RNA-directed RNA polymerase L		PRO_0000361641
Regions
	Domain	1172 – 1369	198	RdRp catalytic

Sequences

	Sequence		Length	Mass (Da)
	Q6IUF8 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 3A5E81BD278CBF72	FASTA	2,209	250,206
	10 20 30 40 50 60 MDEYVQELKG LIRKHIPDRC EFAHQKVTFL SQVHPSPLLT EGFKLLSSLV ELESCEAHAC 70 80 90 100 110 120 QANTDQRFVD VILSDNGILC PTLPKVIPDG FKLTGKTLIL LETFVRVNPD EFEKKWKADM 130 140 150 160 170 180 SKLLNLKHDL QKSGVTLVPI VDGRSNYNNR FVADWVIERM RWLLIEILKA SKSMLEIDIE 190 200 210 220 230 240 DQEYQRLIHS LSNVKNQSLG LENLEHLKRN SLDYDERLNE SLFIGLKGDI RESTVREELI 250 260 270 280 290 300 KLKMWFKDEV FSKGLGKFKL TDRRELLESL SSLGAHLDSD VSSCPFCNNK LMEIVYNVTF 310 320 330 340 350 360 SSVERTDGAA TVDQQFSTTH TNIEKHYLSV LSLCNKIKGL KVFNTRRNTL LFLDLIMVNL 370 380 390 400 410 420 MVDISESCQD AIESLRKSGL IVGQMVMLVN DRVLDILEAI KLIRKKIGTN PNWVKNCSKI 430 440 450 460 470 480 LERSHPEIWL QLNTLIRQPD FNSLISIAQY LVSDRPIMRY SVERGSDKIC RHKLFQEMSS 490 500 510 520 530 540 FEQMRLFKTL SSISLSLINS MKTSFSSRLL VNEREFSKYF GNVRLRECYA QRFYLAESLV 550 560 570 580 590 600 GFLFYQKTGE RSRCYSVYLS DNGVMSEQGS FYCDPKRFFL PVFSDEVLAG MCEEMTSWLD 610 620 630 640 650 660 FDTGLMNDTG PILRLLVLAI LCSPSKRNQT FLQGLRYFLM AFANQIHHID LISKLVVECK 670 680 690 700 710 720 SSSEVVVQRL AVGLFIRLLG GESDASSFFS RRFKYLLNVS YLCHLITKET PDRLTDQIKC 730 740 750 760 770 780 FEKFIEPKVK FGCAVVNPSL NGKLTVDQED IMINGLKKFF SKSLRDTEDV QTPGVCKELL 790 800 810 820 830 840 NYCVSLFNRG KLKVSGELKN NPFRPNITST ALDLSSNKSV VIPKLDELGN ILSTYDKEKL 850 860 870 880 890 900 VSACVSSMAE RFKTKGRYNL DPESTDYLIL KNLTGLVSAG PKAKSSQEEL SLMYETLTEE 910 920 930 940 950 960 QVESFNEIKY DVQVALAKMA DNSVNTRIKN LGRADNSVKN GNNPLDNLWS PFGVMKEIRA 970 980 990 1000 1010 1020 EVSLHEVKDF DPDVLPSDVY KELCDAVYKS SEKCNFFLEE VLDVCPLGLL LKNLTTSSYM 1030 1040 1050 1060 1070 1080 EEEYFMCFKY LLIQGHFDQK LGSYEHKSRS RLGFTDETLR LKDEVRLSIR ESNSEAIADK 1090 1100 1110 1120 1130 1140 LDKSYFTNAA LRNLCFYSED SPTEFTSISS NSGNLKFGLS YKEQVGSNRE LYVGDLNTKL 1150 1160 1170 1180 1190 1200 MTRLVEDFSE AVGNSMKYTC LNSEKEFERA ICDMKMAVNN GDLSCSYDHS KWGPTMSPAL 1210 1220 1230 1240 1250 1260 FLALLQMLEL RTPVDRSKID LDSVKSILKW HLHKVVEVPI NVAEAYCIGK LKRSLGLMGC 1270 1280 1290 1300 1310 1320 GSTSLSEEFF HQTMQLSGQI PSHIMSVLDM GQGILHNTSD LYGLITEQFL CYALDLLYDV 1330 1340 1350 1360 1370 1380 IPVSYTSSDD QITLVKTPSL DIEGGSDAAE WLEMICFHEF LSSKLNKFVS PKSVIGTFVA 1390 1400 1410 1420 1430 1440 EFKSRFFVMG EETPLLTKFV SAALHNVKCK TPTQLSETID TICDQCIANG VSTKIVARIS 1450 1460 1470 1480 1490 1500 KRVNQLIRYS GYGDTPFGAI EDQDVKDWVD GSRGYRLQRK IEAIFYDDKE TSFIRNCARK 1510 1520 1530 1540 1550 1560 VFNDIKRGRI FEENLINLIG RGGDEALTGF LQYAGCSEQE VNRVLNYRWV NLSSFGDLRL 1570 1580 1590 1600 1610 1620 VLRTKLMTSR RVLEREEVPT LIKTLQSKLS RNFTKGVKKI LAESINKSAF QSSVASGFIG 1630 1640 1650 1660 1670 1680 FCKSMGSKCV RDGKGGFLYI KEVYSGINVC ICEICALKPK IIYCNDSLNK VSQFSKPILW 1690 1700 1710 1720 1730 1740 DYFSLVLTNA CELGEWVFST VKEPQKPLVL NNQNFFWAVK PKVVRQIEDQ LGMNHVLQSI 1750 1760 1770 1780 1790 1800 RRNYPVLFDE HLAPFMNDLQ VSRTMDSGRL KFLDVCIALD MMNENLGIIS HLLKTRDNSV 1810 1820 1830 1840 1850 1860 YIVKQSDCAL AHIRQSSYTD WELGLSPQQI CTNFKTQLVL SSMVNPLVLS TSCLKSFFWF 1870 1880 1890 1900 1910 1920 NEVLELEDDS QIELAELTDF ALMVKNQNVS RAMFVEDIAM GYVVSNFEGV RISLSNVMVD 1930 1940 1950 1960 1970 1980 GVQLPPKEKA PDVGVLFGLK AENVIVGLVV QIDHVRMSTK FKLRRKMVYS FSLECTMDVG 1990 2000 2010 2020 2030 2040 DIQNKEVILK VVAVDQSVSG SGGNHMLLDG VPVIASLPLF TGQASFDLAA MLIESNLAGS 2050 2060 2070 2080 2090 2100 NDNFLMSNVT LDLGGFSPEL SDKYSYRLSG PENQEDPLVL KDGAFYVGGE RLSTYKVELT 2110 2120 2130 2140 2150 2160 GDLVVKALGA LEDDEGVVSM LHQLWPYLKA TSQVILFQQE DFTIVHDLYK IQLTKSIESF 2170 2180 2190 2200 GEWIEFTNFK VAYSKSLKEL VISDTQGSFR LKGVMCRPLA NTLQVEDIE

References

[1]	Hajjaj A., Chain P.S.G., Smith K.L., Imbro P.M., Malfatti S.A. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Chicava.
[2]	Jahrling P.B., Geisbert J., Ibrahim M.S. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Chicava.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY624354 Genomic RNA. Translation: AAT45080.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR014386. RNA-dir_pol_arenavirus. IPR007099. RNA-dir_pol_NSvirus. IPR010453. RNA_pol_arenavir. [Graphical view]
Pfam	PF06317. Arena_RNA_pol. 1 hit. [Graphical view]
PIRSF	PIRSF000836. L_ArenaV. 1 hit.
PROSITE	PS50525. RDRP_SSRNA_NEG_SEG. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

L_MACHU

Accession

Primary (citable) accession number: Q6IUF8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	July 5, 2004
Last modified:	June 28, 2011
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program