Q6ITT3 (MGT4C_PIG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase C
EC=2.4.1.145

Alternative name(s):
N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase IVc
Short name=GnT-IVc
Short name=N-acetylglucosaminyltransferase IVc
UDP-N-acetylglucosamine: alpha-1,3-D-mannoside beta-1,4-N-acetylglucosaminyltransferase IVc

Gene names

Name:

MGAT4C

Organism

Sus scrofa (Pig) [Complete proteome]

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	478 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Glycosyltransferase that participates in the transfer of N-acetylglucosamine (GlcNAc) to the core mannose residues of N-linked glycans. Catalyzes the formation of the GlcNAcbeta1-4 branch on the GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans. Essential for the production of tri- and tetra-antennary N-linked sugar chains. Does not catalyze the transfer of GlcNAc to the Manalpha1-6 arm to form GlcNAcBeta1-4Manalpha1-6 linkage ('GnT-VI' activity) By similarity.
Catalytic activity	UDP-N-acetyl-D-glucosamine + 3-(2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 3-(2,4-bis(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R.
Cofactor	Divalent metal cations By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase 54 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Ligand	Metal-binding
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alpha-1,3-mannosylglycoprotein 4-beta-N-acetylglucosaminyltransferase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 478	478	Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase C		PRO_0000288599
Regions
	Topological domain	1 – 23	23	Cytoplasmic Potential
	Transmembrane	24 – 44	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	45 – 478	434	Lumenal Potential
Amino acid modifications
	Glycosylation	84	1	N-linked (GlcNAc...) Potential
	Glycosylation	215	1	N-linked (GlcNAc...) Potential
	Glycosylation	348	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q6ITT3 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: AA4DDEF8554ACE25	FASTA	478	56,312
	10 20 30 40 50 60 MFKFHQVKHI FEILDKMRCL RKRFTVSFLG VLVIFLLFMN LYIEDSYVLE GDKQLVRETS 70 80 90 100 110 120 IHQLNPERYV HTFKYLSNFS GTINVTYRYL AVMPFQRKRF LTIGLSSVRR KKGNYLLETI 130 140 150 160 170 180 KSIFEQSSYE ELKEISVVVH LADFNSSWRE VMVQDITQKF AHHIIAGRLM VIHAPEEYYP 190 200 210 220 230 240 VLNGLKRNYN DPEDRVRFRS KQNVDYAFLL NFCANISDYY VMLEDDVRCS KNFLTAIKKV 250 260 270 280 290 300 ITSLQGTYWV TLEFSKLGYI GKLYHSHDLP RLAHFLLMFY QEMPCDWLLT HFRGLLAQKN 310 320 330 340 350 360 VIRFKPSLFQ HMGYYSSYKG TENKLKDDDF EEELIDLPDN PPASLYTNMS VFENYDASKA 370 380 390 400 410 420 YSSVDGYFWG KPPSTGDVFV VVFENPVIIK KIKVSTGTED RQNDILHHGA LDVGGYIRYF 430 440 450 460 470 KQNRQCITYI RLGEFKNGNF EVSDVNQKIP FDVHCMRIHV TKTQKEWLII RSISIWTS

References

[1]	"Characterization of porcine UDP-N-acetylglucosamine:a-1,3-D-mannoside beta-1,4-N-acetylglucosaminyltransferase IV-homolog (HGNT-IV-H)." Kim J.G., Vallet J.L., Nonneman D., Rohrer G.A., Christenson R.K. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY626239 mRNA. Translation: AAT45730.1.
RefSeq	NP_001001859.1. NM_001001859.1.
UniGene	Ssc.42784.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q6ITT3.
Protein family/group databases
CAZy	GT54. Glycosyltransferase Family 54.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	414851.
KEGG	ssc:414851.
Organism-specific databases
CTD	25834.
Phylogenomic databases
GeneTree	ENSGT00550000074578.
HOVERGEN	HBG103174.
OrthoDB	EOG40GCQQ.
Family and domain databases
InterPro	IPR006759. Glyco_transf_54. [Graphical view]
KO	K13748.
PANTHER	PTHR12062. Glyco_transf_54. 1 hit.
Pfam	PF04666. Glyco_transf_54. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MGT4C_PIG

Accession

Primary (citable) accession number: Q6ITT3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	July 5, 2004
Last modified:	November 16, 2011
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program