Reviewed, UniProtKB/Swiss-Prot Q6ITT3 (MGT4C_PIG)

Names and origin

Protein names

Recommended name:
    Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase C
    EC=2.4.1.145
Alternative name(s):
    UDP-N-acetylglucosamine: alpha-1,3-D-mannoside beta-1,4-N-acetylglucosaminyltransferase IVc
    N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase IVc
      Short name=N-acetylglucosaminyltransferase IVc
      Short name=GnT-IVc

Gene names

Name:

MGAT4C

Organism

Sus scrofa (Pig)

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	478 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Glycosyltransferase that participates in the transfer of N-acetylglucosamine (GlcNAc) to the core mannose residues of N-linked glycans. Catalyzes the formation of the GlcNAcbeta1-4 branch on the GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans. Essential for the production of tri- and tetra-antennary N-linked sugar chains. Does not catalyze the transfer of GlcNAc to the Manalpha1-6 arm to form GlcNAcBeta1-4Manalpha1-6 linkage ('GnT-VI' activity) By similarity.
Catalytic activity	UDP-N-acetyl-D-glucosamine + 3-(2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 3-(2,4-bis(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R.
Cofactor	Divalent metal cations By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase 54 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Ligand	Metal-binding
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	alpha-1,3-mannosylglycoprotein 4-beta-N-acetylglucosaminyltransferase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 478	478	Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase C		PRO_0000288599
Regions
	Topological domain	1 – 23	23	Cytoplasmic Potential
	Transmembrane	24 – 44	21	Signal-anchor for type II membrane protein Potential
	Topological domain	45 – 478	434	Lumenal Potential
Amino acid modifications
	Glycosylation	84	1	N-linked (GlcNAc...) Potential
	Glycosylation	215	1	N-linked (GlcNAc...) Potential
	Glycosylation	348	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q6ITT3-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: AA4DDEF8554ACE25	FASTA	478	56,312
	10 20 30 40 50 60 MFKFHQVKHI FEILDKMRCL RKRFTVSFLG VLVIFLLFMN LYIEDSYVLE GDKQLVRETS 70 80 90 100 110 120 IHQLNPERYV HTFKYLSNFS GTINVTYRYL AVMPFQRKRF LTIGLSSVRR KKGNYLLETI 130 140 150 160 170 180 KSIFEQSSYE ELKEISVVVH LADFNSSWRE VMVQDITQKF AHHIIAGRLM VIHAPEEYYP 190 200 210 220 230 240 VLNGLKRNYN DPEDRVRFRS KQNVDYAFLL NFCANISDYY VMLEDDVRCS KNFLTAIKKV 250 260 270 280 290 300 ITSLQGTYWV TLEFSKLGYI GKLYHSHDLP RLAHFLLMFY QEMPCDWLLT HFRGLLAQKN 310 320 330 340 350 360 VIRFKPSLFQ HMGYYSSYKG TENKLKDDDF EEELIDLPDN PPASLYTNMS VFENYDASKA 370 380 390 400 410 420 YSSVDGYFWG KPPSTGDVFV VVFENPVIIK KIKVSTGTED RQNDILHHGA LDVGGYIRYF 430 440 450 460 470 KQNRQCITYI RLGEFKNGNF EVSDVNQKIP FDVHCMRIHV TKTQKEWLII RSISIWTS

References

[1]	"Characterization of porcine UDP-N-acetylglucosamine:a-1,3-D-mannoside beta-1,4-N-acetylglucosaminyltransferase IV-homolog (HGNT-IV-H)." Kim J.G., Vallet J.L., Nonneman D., Rohrer G.A., Christenson R.K. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY626239 mRNA. Translation: AAT45730.1.
RefSeq	NP_001001859.1.
UniGene	Ssc.42784
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT54. Glycosyltransferase Family 54.
Genome annotation databases
GeneID	414851.
KEGG	ssc:414851.
Organism-specific databases
CTD	414851.
Phylogenomic databases
HOVERGEN	Q6ITT3.
Enzyme and pathway databases
BRENDA	2.4.1.145. 249.
Family and domain databases
InterPro	IPR006759. Glyco_transf_54. [Graphical view]
PANTHER	PTHR12062. Glyco_transf_54. 1 hit.
Pfam	PF04666. Glyco_transf_54. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MGT4C_PIG

Accession

Primary (citable) accession number: Q6ITT3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	July 5, 2004
Last modified:	October 13, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)