Q6ISU1 (PTCRA_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pre T-cell antigen receptor alpha
Short name=pT-alpha
Short name=pTa

Alternative name(s):
pT-alpha-TCR

Gene names

Name:

PTCRA

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	281 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The pre-T-cell receptor complex (composed of PTCRA, TCRB and the CD3 complex) regulates early T-cell development By similarity.
Subunit structure	Heterodimer with TCRB; disulfide linked. This heterodimer assembles with CD3 proteins into a signaling-competent pre-T-cell receptor complex. Interacts with RHBDD1. Ref.6 Ref.7 Ref.8
Subcellular location	Membrane; Single-pass type I membrane protein Potential.
Tissue specificity	Expressed in immature but not mature T-cells. Also found in CD34+ cells from peripheral blood, CD34+ precursors from umbilical cord blood and adult bone marrow. Ref.1 Ref.6
Developmental stage	Expressed in fetal life in CD34+ progenitors present in the liver at 18 weeks of gestation but is absent in CD34+ precursors from fetal bone marrow at any developmental stage up to 22 weeks. Ref.6

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Signal Transmembrane Transmembrane helix
Molecular function	Receptor
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of thymocyte apoptotic process Inferred from electronic annotation. Source: Compara
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q6ISU1-1)

Also known as: pTalpha-1;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q6ISU1-2)

Also known as: pTalpha-2;

The sequence of this isoform differs from the canonical sequence as follows:
     20-126: Missing.

Isoform 3 (identifier: Q6ISU1-3)

The sequence of this isoform differs from the canonical sequence as follows:
     21-59: VGGTPFPSLAPPIMLLVDGKQQMVVVCLVLDVAPPGLDS → PVSFPSSPEAATTG

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 23

23

Potential

Chain

24 – 281

258

Pre T-cell antigen receptor alpha

PRO_0000319108

Regions

Topological domain

24 – 146

123

Extracellular Potential

Transmembrane

147 – 167

21

Helical; Potential

Topological domain

168 – 281

114

Cytoplasmic Potential

Amino acid modifications

Glycosylation

67

1

N-linked (GlcNAc...) Ref.8

Disulfide bond

47 ↔ 107

Ref.8

Disulfide bond

135

Interchain (with TCRB) Probable

Natural variations

Alternative sequence

20 – 126

107

Missing in isoform 2.

VSP_031445

Alternative sequence

21 – 59

39

VGGTP…PGLDS → PVSFPSSPEAATTG in isoform 3.

VSP_031446

Natural variant

106

1

V → I. Ref.5
Corresponds to variant rs9471966 [ dbSNP | Ensembl ].

VAR_038957

Natural variant

183

1

A → T.
Corresponds to variant rs36111725 [ dbSNP | Ensembl ].

VAR_038958

Experimental info

Sequence conflict

256

1

A → R in AAB06194. Ref.1

Sequence conflict

256

1

A → R in AAC83346. Ref.2

Sequence conflict

256

1

A → R in AAF89556. Ref.3

Sequence conflict

256

1

A → R in AAB18373. Ref.6

Sequence conflict

269

1

A → R in AAB06194. Ref.1

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

281

Helix Strand Turn

Details...

Beta strand

33 – 52

20

Beta strand

62 – 64

3

Beta strand

66 – 68

3

Beta strand

79 – 81

3

Turn

82 – 84

3

Beta strand

85 – 95

11

Helix

96 – 100

5

Beta strand

104 – 109

6

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (pTalpha-1) [UniParc]. Last modified July 5, 2004. Version 1. Checksum: A49B86A151619B9F	FASTA	281	29,266
	10 20 30 40 50 60 MAGTWLLLLL ALGCPALPTG VGGTPFPSLA PPIMLLVDGK QQMVVVCLVL DVAPPGLDSP 70 80 90 100 110 120 IWFSAGNGSA LDAFTYGPSP ATDGTWTNLA HLSLPSEELA SWEPLVCHTG PGAEGHSRST 130 140 150 160 170 180 QPMHLSGEAS TARTCPQEPL RGTPGGALWL GVLRLLLFKL LLFDLLLTCS CLCDPAGPLP 190 200 210 220 230 240 SPATTTRLRA LGSHRLHPAT ETGGREATSS PRPQPRDRRW GDTPPGRKPG SPVWGEGSYL 250 260 270 280 SSYPTCPAQA WCSRSALRAP SSSLGAFFAG DLPPPLQAGA A
	Isoform 2 (pTalpha-2) [UniParc]. Checksum: A7EAEAD3884692A3	FASTA	174	18,222
	10 20 30 40 50 60 MAGTWLLLLL ALGCPALPTG EASTARTCPQ EPLRGTPGGA LWLGVLRLLL FKLLLFDLLL 70 80 90 100 110 120 TCSCLCDPAG PLPSPATTTR LRALGSHRLH PATETGGREA TSSPRPQPRD RRWGDTPPGR 130 140 150 160 170 KPGSPVWGEG SYLSSYPTCP AQAWCSRSAL RAPSSSLGAF FAGDLPPPLQ AGAA
	Isoform 3 [UniParc]. Checksum: 815805A8311B0B3B	FASTA	256	26,638
	10 20 30 40 50 60 MAGTWLLLLL ALGCPALPTG PVSFPSSPEA ATTGPIWFSA GNGSALDAFT YGPSPATDGT 70 80 90 100 110 120 WTNLAHLSLP SEELASWEPL VCHTGPGAEG HSRSTQPMHL SGEASTARTC PQEPLRGTPG 130 140 150 160 170 180 GALWLGVLRL LLFKLLLFDL LLTCSCLCDP AGPLPSPATT TRLRALGSHR LHPATETGGR 190 200 210 220 230 240 EATSSPRPQP RDRRWGDTPP GRKPGSPVWG EGSYLSSYPT CPAQAWCSRS ALRAPSSSLG 250 AFFAGDLPPP LQAGAA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and comparative analysis of the human pre-T-cell receptor alpha-chain gene." Del Porto P., Bruno L., Mattei M.-G., von Boehmer H., Saint-Ruf C. Proc. Natl. Acad. Sci. U.S.A. 92:12105-12109(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY. Tissue: Thymus.
[2]	"Genomic structure of the human pre-T cell receptor alpha chain and expression of two mRNA isoforms." Saint-Ruf C., Lechner O., Feinberg J., von Boehmer H. Eur. J. Immunol. 28:3824-3831(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS 1 AND 2). Tissue: Lymphoblast.
[3]	"Alternatively spliced human pre-T alpha chain, mRNA." Ramiro A.R., Toribio M.L. Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2 AND 3).
[4]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT ILE-106.
[6]	"Regulation of pre-T cell receptor (pT alpha-TCR beta) gene expression during human thymic development." Ramiro A.R., Trigueros C., Marquez C., San Millan J.L., Toribio M.L. J. Exp. Med. 184:519-530(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 13-281 (ISOFORM 1), SUBUNIT, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Tissue: Thymus.
[7]	"Ubiquitin-Dependent Intramembrane Rhomboid Protease Promotes ERAD of Membrane Proteins." Fleig L., Bergbold N., Sahasrabudhe P., Geiger B., Kaltak L., Lemberg M.K. Mol. Cell 47:558-569(2012) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH RHBDD1.
[8]	"The structural basis for autonomous dimerization of the pre-T-cell antigen receptor." Pang S.S., Berry R., Chen Z., Kjer-Nielsen L., Perugini M.A., King G.F., Wang C., Chew S.H., La Gruta N.L., Williams N.K., Beddoe T., Tiganis T., Cowieson N.P., Godfrey D.I., Purcell A.W., Wilce M.C., McCluskey J., Rossjohn J. Nature 467:844-848(2010) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 17-135, SUBUNIT, DISULFIDE BOND, GLYCOSYLATION AT ASN-67.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U36759 mRNA. Translation: AAB06194.1.
AF084941 Genomic DNA. Translation: AAC83346.1.
AF101436 mRNA. Translation: AAF21890.1.
AF165312 mRNA. Translation: AAF89556.1.
AL035587 Genomic DNA. Translation: CAI21484.2.
BC069336 mRNA. Translation: AAH69336.1.
BC100771 mRNA. Translation: AAI00772.1.
BC100772 mRNA. Translation: AAI00773.1.
BC100773 mRNA. Translation: AAI00774.1.
BC100774 mRNA. Translation: AAI00775.1.
BC153829 mRNA. Translation: AAI53830.1.
U38996 mRNA. Translation: AAB18373.1.

IPI

IPI00514715.
IPI00884912.
IPI00885032.

RefSeq

NP_001230097.1. NM_001243168.1.
NP_001230098.1. NM_001243169.1.
NP_001230099.1. NM_001243170.1.
NP_612153.2. NM_138296.2.

UniGene

Hs.169002.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3OF6	X-ray	2.80	D/E/F	17-135	[»]

ProteinModelPortal

Q6ISU1.

ModBase

Search...

Protein-protein interaction databases

MINT

MINT-7219046.

STRING

9606.ENSP00000304447.

Polymorphism databases

DMDM

74736631.

Proteomic databases

PaxDb

Q6ISU1.

PRIDE

Q6ISU1.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000304672; ENSP00000304447; ENSG00000171611.
ENST00000441198; ENSP00000409550; ENSG00000171611.
ENST00000446507; ENSP00000392288; ENSG00000171611.

GeneID

171558.

KEGG

hsa:171558.

UCSC

uc003osx.3. human.
uc010jxy.3. human.
uc010jxz.3. human.

Organism-specific databases

CTD

171558.

GeneCards

GC06P042883.

HGNC

HGNC:21290. PTCRA.

MIM

606817. gene.

neXtProt

NX_Q6ISU1.

PharmGKB

PA134993184.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG25752.

HOGENOM

HOG000115761.

HOVERGEN

HBG061856.

InParanoid

Q6ISU1.

KO

K06056.

OMA

SPVWEEG.

OrthoDB

EOG4WSWC1.

PhylomeDB

Q6ISU1.

Gene expression databases

Bgee

Q6ISU1.

CleanEx

HS_PTCRA.

Genevestigator

Q6ISU1.

Family and domain databases

ProtoNet

Search...

Other

EvolutionaryTrace

Q6ISU1.

GenomeRNAi

171558.

NextBio

89289.

SOURCE

Search...

Entry information

Entry name

PTCRA_HUMAN

Accession

Primary (citable) accession number: Q6ISU1
Secondary accession number(s): Q5TFZ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	July 5, 2004
Last modified:	May 29, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.