Reviewed, UniProtKB/Swiss-Prot Q6IRI9 (FMO2_RAT)

Names and origin

Protein names

Recommended name:
    Dimethylaniline monooxygenase [N-oxide-forming] 2
    EC=1.14.13.8
Alternative name(s):
    Pulmonary flavin-containing monooxygenase 2
      Short name=FMO 2
    Dimethylaniline oxidase 2

Gene names

Name:

Fmo2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	535 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	This protein is involved in the oxidative metabolism of a variety of xenobiotics such as drugs and pesticides By similarity.
Catalytic activity	N,N-dimethylaniline + NADPH + O2 = N,N-dimethylaniline N-oxide + NADP+ + H2O.
Cofactor	FAD By similarity. Magnesium By similarity.
Subcellular location	Microsome membrane By similarity. Endoplasmic reticulum membrane By similarity.
Sequence similarities	Belongs to the FMO family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Domain	Transmembrane
Ligand	FAD Flavoprotein Magnesium NADP
Molecular function	Monooxygenase Oxidoreductase
PTM	Acetylation
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW intrinsic to endoplasmic reticulum membrane Inferred from electronic annotation. Source: InterPro microsome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro NADP or NADPH binding Inferred from electronic annotation. Source: InterPro flavin-containing monooxygenase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 535	534	Dimethylaniline monooxygenase [N-oxide-forming] 2		PRO_0000147651
Regions
	Nucleotide binding	9 – 14	6	FAD Potential
	Nucleotide binding	191 – 196	6	NADP Potential
Amino acid modifications
	Modified residue	2	1	N-acetylvaline By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6IRI9-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 4BDABD847733F639	FASTA	535	60,899
	10 20 30 40 50 60 MVKKVAVIGA GVSGLISLKG CVDEGLEPTC FERTEDIGGL WRFKENVEDG RASIYHSVIT 70 80 90 100 110 120 NTSKEMSCFS DFPMPEDFPN FLHNSKLLEY FRIFAKKFDL LKYIQFQTTV ISVKKRPDFA 130 140 150 160 170 180 SSGQWDVYVQ SNGKEQRAVF DAVMVCSGHH IQPHLPLKSF PGIERFQGQY FHSRQYKHPV 190 200 210 220 230 240 GYEGKRILVV GIGNSAADIA SELSKRAAQV FVSTRHGSWV LSRISEDGYP WDMVFHTRFS 250 260 270 280 290 300 SMLRNVLPRT VVKWMMERQM NRWFNHENYG LVPQNKYLMK EPVLNDDLPS RLLYGAIKVK 310 320 330 340 350 360 TRVKELTETA VVFEDGTVEE DVDVIVFATG YTFSFPFLED SLVKVEDNKV SLYKAMFPPH 370 380 390 400 410 420 LEKPTLACIG LIQPLGSIFP TVELQARWAT RVFKGVCRLP SETTMMADIA ERNEKRIDLF 430 440 450 460 470 480 GKSQSQILQT NYIDYLDELA LEIGAKPDFI SLLFKDPKLA VKLYFGPCNS YQYRLVGPGQ 490 500 510 520 530 WEGARNAILT QKQRILKPLK TRTLQTSASA PVSFLIKVLG LLAIVLAFFF KLHGF

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC070904 mRNA. Translation: AAH70904.1.
IPI	IPI00421926.
RefSeq	NP_653338.2.
UniGene	Rn.3928
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSRNOG00000003510. Rattus norvegicus. [Contig view]
GeneID	246245.
KEGG	rno:246245.
Organism-specific databases
RGD	628600. Fmo2.
Phylogenomic databases
HOVERGEN	Q6IRI9.
Enzyme and pathway databases
BRENDA	1.14.13.8. 248.
Gene expression databases
ArrayExpress	Q6IRI9.
GermOnline	ENSRNOG00000003510. Rattus norvegicus.
Family and domain databases
InterPro	IPR012143. dManiline_mOase. IPR000960. Flavin_mOase. IPR002254. Flavin_mOase_2. [Graphical view]
Pfam	PF00743. FMO-like. 1 hit. [Graphical view]
PIRSF	PIRSF000332. FMO. 1 hit.
PRINTS	PR00370. FMOXYGENASE. PR01122. FMOXYGENASE2.
ProDom	PD000139. FAD_pyr_redox. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...
Other Resources
NextBio	623556.

Entry information

Entry name

FMO2_RAT

Accession

Primary (citable) accession number: Q6IRI9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2005
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 47 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)