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Protein

Tumor susceptibility gene 101 protein

Gene

Tsg101

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Required for the exosomal release of SDCBP, CD63 and syndecan (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Growth regulation, Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-RNO-917729. Endosomal Sorting Complex Required For Transport (ESCRT).

Names & Taxonomyi

Protein namesi
Recommended name:
Tumor susceptibility gene 101 protein
Alternative name(s):
ESCRT-I complex subunit TSG101
Gene namesi
Name:Tsg101
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi3909. Tsg101.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • early endosome Source: Ensembl
  • endosome membrane Source: UniProtKB
  • ESCRT I complex Source: UniProtKB
  • extracellular exosome Source: Ensembl
  • late endosome membrane Source: UniProtKB-SubCell
  • nucleolus Source: Ensembl
  • nucleus Source: RGD
  • plasma membrane Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 391390Tumor susceptibility gene 101 proteinPRO_0000082608Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei221 – 2211PhosphothreonineBy similarity

Post-translational modificationi

Monoubiquitinated at multiple sites by LRSAM1 and by MGRN1. Ubiquitination inactivates it, possibly by regulating its shuttling between an active membrane-bound protein and an inactive soluble form. Ubiquitination by MGRN1 requires the presence of UBE2D1 (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PRIDEiQ6IRE4.

PTM databases

iPTMnetiQ6IRE4.
PhosphoSiteiQ6IRE4.

Expressioni

Gene expression databases

ExpressionAtlasiQ6IRE4. baseline and differential.
GenevisibleiQ6IRE4. RN.

Interactioni

Subunit structurei

Component of the ESCRT-I complex (endosomal sorting complex required for transport I) which consists of TSG101, VPS28, a VPS37 protein (VPS37A to -D) and MVB12A or MVB12B in a 1:1:1:1 stoechiometry. Interacts with VPS37A, VPS37B and VPS37C. Component of an ESCRT-I complex (endosomal sorting complex required for transport I) which consists of TSG101, VPS28, VPS37A and UBAP1 in a 1:1:1:1 stoechiometry. Interacts with ubiquitin, stathmin, GMCL, DMAP1 and AATF (By similarity). Interacts with HGS; the interaction mediates the association with the ESCRT-0 complex. Interacts with GGA1 and GGA3. Interacts (via UEV domain) with PDCD6IP/AIP1. Interacts with VPS28, SNF8 and VPS36. Self-associates. Interacts with MVB12A; the association appears to be mediated by the TSG101-VPS37 binary subcomplex. Interacts with VPS37D. Interacts with LRSAM1. Interacts with CEP55; the interaction is required for cytokinesis (By similarity). Interacts with PDCD6 (By similarity). Interacts with MGRN1 (By similarity).By similarity

Protein-protein interaction databases

BioGridi254034. 2 interactions.
IntActiQ6IRE4. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ6IRE4.
SMRiQ6IRE4. Positions 2-145.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 145144UEVPROSITE-ProRule annotationAdd
BLAST
Domaini323 – 39169SBPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni159 – 1635Interaction with CEP55By similarity

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili238 – 31780Sequence analysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi321 – 3244PTAP motif

Domaini

The UEV domain is required for the interaction of the complex with ubiquitin.By similarity
The coiled coil domain may interact with stathmin.By similarity

Sequence similaritiesi

Contains 1 SB (steadiness box) domain.PROSITE-ProRule annotation
Contains 1 UEV (ubiquitin E2 variant) domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

GeneTreeiENSGT00530000064004.
HOGENOMiHOG000247008.
HOVERGENiHBG057450.
InParanoidiQ6IRE4.
KOiK12183.
OMAiPVMDSYV.
OrthoDBiEOG091G0HRA.
PhylomeDBiQ6IRE4.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR017916. SB_dom.
IPR016135. UBQ-conjugating_enzyme/RWD.
IPR008883. UEV_N.
[Graphical view]
PfamiPF05743. UEV. 1 hit.
PF09454. Vps23_core. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS51312. SB. 1 hit.
PS51322. UEV. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6IRE4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVSESQLKK MMSKYKYRDL TVRQTVNVIA MYKDLKPVLD SYVFNDGSSR
60 70 80 90 100
ELVNLTGTIP VRYRGNIYNI PICLWLLDTY PYNPPICFVK PTSSMTIKTG
110 120 130 140 150
KHVDANGKIY LPYLHDWKHP RSELLELIQI MIVIFGEEPP VFSRPTVSAS
160 170 180 190 200
YPPYTAAGPP NTSYLPSMPS GISAYPSGYP PNPSGYPGCP YPPAGPYPAT
210 220 230 240 250
TSSQYPSQPP VTTAGPSRDG TISEDTIRAS LISAVSDKLR WRMKEEMDGA
260 270 280 290 300
QAELNALKRT EEDLKKGHQK LEEMVTRLDQ EVAEVDKNIE LLKKKDEELS
310 320 330 340 350
SALEKMENQS ENNDIDEVII PTAPLYKQIL NLYAEENAIE DTIFYLGEAL
360 370 380 390
RRGVIDLDVF LKHVRLLSRK QFQLRALMQK ARKTAGLSDL Y
Length:391
Mass (Da):44,078
Last modified:July 5, 2004 - v1
Checksum:i9403128B347CCF73
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti45 – 451N → S in AAP45008 (PubMed:14761944).Curated
Sequence conflicti89 – 891V → A in AAP45008 (PubMed:14761944).Curated
Sequence conflicti150 – 1501S → F in AAP45008 (PubMed:14761944).Curated
Sequence conflicti174 – 1741A → V in AAP45008 (PubMed:14761944).Curated
Sequence conflicti311 – 3111E → D in AAP45008 (PubMed:14761944).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY293306 mRNA. Translation: AAP45008.1.
BC070951 mRNA. Translation: AAH70951.1.
RefSeqiNP_853659.2. NM_181628.2.
UniGeneiRn.7410.

Genome annotation databases

EnsembliENSRNOT00000018194; ENSRNOP00000018194; ENSRNOG00000013381.
GeneIDi292925.
KEGGirno:292925.
UCSCiRGD:3909. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY293306 mRNA. Translation: AAP45008.1.
BC070951 mRNA. Translation: AAH70951.1.
RefSeqiNP_853659.2. NM_181628.2.
UniGeneiRn.7410.

3D structure databases

ProteinModelPortaliQ6IRE4.
SMRiQ6IRE4. Positions 2-145.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi254034. 2 interactions.
IntActiQ6IRE4. 1 interaction.

PTM databases

iPTMnetiQ6IRE4.
PhosphoSiteiQ6IRE4.

Proteomic databases

PRIDEiQ6IRE4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000018194; ENSRNOP00000018194; ENSRNOG00000013381.
GeneIDi292925.
KEGGirno:292925.
UCSCiRGD:3909. rat.

Organism-specific databases

CTDi7251.
RGDi3909. Tsg101.

Phylogenomic databases

GeneTreeiENSGT00530000064004.
HOGENOMiHOG000247008.
HOVERGENiHBG057450.
InParanoidiQ6IRE4.
KOiK12183.
OMAiPVMDSYV.
OrthoDBiEOG091G0HRA.
PhylomeDBiQ6IRE4.

Enzyme and pathway databases

ReactomeiR-RNO-917729. Endosomal Sorting Complex Required For Transport (ESCRT).

Miscellaneous databases

PROiQ6IRE4.

Gene expression databases

ExpressionAtlasiQ6IRE4. baseline and differential.
GenevisibleiQ6IRE4. RN.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR017916. SB_dom.
IPR016135. UBQ-conjugating_enzyme/RWD.
IPR008883. UEV_N.
[Graphical view]
PfamiPF05743. UEV. 1 hit.
PF09454. Vps23_core. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS51312. SB. 1 hit.
PS51322. UEV. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTS101_RAT
AccessioniPrimary (citable) accession number: Q6IRE4
Secondary accession number(s): Q7TSE5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 5, 2004
Last modified: September 7, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.