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Protein

G-protein-signaling modulator 1

Gene

Gpsm1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Guanine nucleotide dissociation inhibitor (GDI) which functions as a receptor-independent activator of heterotrimeric G-protein signaling. Keeps G(i/o) alpha subunit in its GDP-bound form thus uncoupling heterotrimeric G-proteins signaling from G protein-coupled receptors. Controls spindle orientation and asymmetric cell fate of cerebral cortical progenitors. May also be involved in macroautophagy in intestinal cells. May play a role in drug addiction.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Neurogenesis

Enzyme and pathway databases

ReactomeiR-MMU-418594. G alpha (i) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
G-protein-signaling modulator 1
Alternative name(s):
Activator of G-protein signaling 3
Gene namesi
Name:Gpsm1
Synonyms:Ags3
ORF Names:C10a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1915089. Gpsm1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Endoplasmic reticulum, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002524031 – 673G-protein-signaling modulator 1Add BLAST673

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei410PhosphoserineCombined sources1
Modified residuei418Omega-N-methylarginineCombined sources1
Modified residuei442PhosphoserineBy similarity1
Modified residuei467PhosphoserineBy similarity1
Modified residuei469PhosphoserineBy similarity1
Modified residuei490PhosphoserineCombined sources1
Modified residuei491PhosphoserineCombined sources1
Modified residuei543PhosphoserineBy similarity1
Modified residuei567PhosphoserineBy similarity1
Modified residuei653PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylation regulates interaction with G(i/o) alpha.1 Publication

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

PaxDbiQ6IR34.
PeptideAtlasiQ6IR34.
PRIDEiQ6IR34.

PTM databases

iPTMnetiQ6IR34.
PhosphoSitePlusiQ6IR34.

Expressioni

Tissue specificityi

Expressed in neural progenitor cells (at protein level).1 Publication

Developmental stagei

Expressed in brain at E14.1 Publication

Gene expression databases

BgeeiENSMUSG00000026930.
ExpressionAtlasiQ6IR34. baseline and differential.
GenevisibleiQ6IR34. MM.

Interactioni

Subunit structurei

Interacts with GNAI1 and GNAI2 preferentially in their GDP-bound state. May also interact with GNAO1. Interacts with INSC/inscuteable and FRMPD1 (By similarity). Interacts with GNAI3. Interacts with STK11/LKB1 and MACF1.By similarity2 Publications

Protein-protein interaction databases

BioGridi212469. 11 interactors.
IntActiQ6IR34. 12 interactors.
MINTiMINT-8305267.
STRINGi10090.ENSMUSP00000067964.

Structurei

3D structure databases

ProteinModelPortaliQ6IR34.
SMRiQ6IR34.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati28 – 61TPR 1Add BLAST34
Repeati66 – 99TPR 2Add BLAST34
Repeati106 – 139TPR 3Add BLAST34
Repeati146 – 178TPR 4Add BLAST33
Repeati180 – 199TPR 5Add BLAST20
Repeati206 – 239TPR 6Add BLAST34
Repeati246 – 279TPR 7Add BLAST34
Repeati286 – 319TPR 8Add BLAST34
Repeati326 – 359TPR 9Add BLAST34
Domaini493 – 515GoLoco 1PROSITE-ProRule annotationAdd BLAST23
Domaini546 – 568GoLoco 2PROSITE-ProRule annotationAdd BLAST23
Domaini594 – 616GoLoco 3PROSITE-ProRule annotationAdd BLAST23
Domaini628 – 650GoLoco 4PROSITE-ProRule annotationAdd BLAST23

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 507Mediates association with membranesBy similarityAdd BLAST507
Regioni361 – 485Interaction with STK11/LKB1By similarityAdd BLAST125

Domaini

The GoLoco domains are essential for the GDI activity toward G(i/o) alpha. The GoLoco domains mediate interaction with G(i/o) alpha (By similarity).By similarity

Sequence similaritiesi

Belongs to the GPSM family.Curated
Contains 4 GoLoco domains.PROSITE-ProRule annotation
Contains 9 TPR repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, TPR repeat

Phylogenomic databases

eggNOGiKOG1130. Eukaryota.
ENOG410XP6N. LUCA.
GeneTreeiENSGT00530000063126.
HOVERGENiHBG051823.
InParanoidiQ6IR34.
KOiK15839.
OMAiQEPGDDF.
OrthoDBiEOG091G04C9.
PhylomeDBiQ6IR34.
TreeFamiTF328344.

Family and domain databases

Gene3Di1.25.40.10. 2 hits.
InterProiIPR024804. G_prot_signal_mod_1.
IPR003109. GoLoco_motif.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR019734. TPR_repeat.
[Graphical view]
PANTHERiPTHR10098:SF36. PTHR10098:SF36. 1 hit.
PfamiPF02188. GoLoco. 4 hits.
[Graphical view]
SMARTiSM00390. GoLoco. 4 hits.
SM00028. TPR. 6 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 2 hits.
PROSITEiPS50877. GOLOCO. 4 hits.
PS50005. TPR. 6 hits.
PS50293. TPR_REGION. 2 hits.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6IR34-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASPAPPVAE ELPGPASRRL YSRMEASCLE LALEGERLCK AGDFKAGVAF
60 70 80 90 100
FEAAVQVGTE DLKTLSAIYS QLGNAYFYLK EYARALQFHK HDLLLARTIG
110 120 130 140 150
DRMGEAKASG NLGNTLKVLG RFDEAIVCCQ RHLDIAQEQG DKVGEARALY
160 170 180 190 200
NIGNVYHAKG KQLSWNAAQD PGHLPPDVRE TLHRASEFYE RNLSLVKELG
210 220 230 240 250
DRAAQGRAYG NLGNTHYLLG NFTEATTFHK ERLAIAKEFG DKAAERRAYS
260 270 280 290 300
NLGNAHIFLG RFDVAAEHYK KTLQLSRQLR DQAVEAQACY SLGNTYTLLQ
310 320 330 340 350
DYERAAEYHL RHLVIAQELA DRVGEGRACW SLGNAYVSMG SPAQALTFAK
360 370 380 390 400
KHLQISQEIG DRNGELTARM NIAHLQLALG RLTSPAAAEK PDLAGYEAQG
410 420 430 440 450
ARPKRTQRLS AETWDLLRLP LDREQNGETH HTGDWRGPGR DSLPLPMRSR
460 470 480 490 500
KYQEGPDAIE RRPREGSHSP LDSADVRVQV PRTGIPRAPS SDEECFFDLL
510 520 530 540 550
SKFQSSRMDD QRCPLEEGQA GAAEATAAPS VEDRAAQSSV TASPQTEEFF
560 570 580 590 600
DLIASSQSRR LDDQRASVGS LPGLRITLNN VGHLRGDGDA QEPGDEFFNM
610 620 630 640 650
LIKYQSSRID DQRCPPPDVL PRGPTMPDED FFSLIQRVQA KRMDEQRVDL
660 670
AGSPEQEASG LPDPQQQCPP GAS
Length:673
Mass (Da):74,362
Last modified:April 20, 2010 - v3
Checksum:i60D284A5340604E7
GO
Isoform 2 (identifier: Q6IR34-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     424-483: Missing.

Show »
Length:613
Mass (Da):67,564
Checksum:iFC2FBD68094DB658
GO
Isoform 3 (identifier: Q6IR34-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-23: Missing.

Show »
Length:650
Mass (Da):71,927
Checksum:iC6F8183601175AD7
GO
Isoform 4 (identifier: Q6IR34-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-22: MASPAPPVAEELPGPASRRLYS → MLASAMEGQP...VQKMRNLQRK
     251-271: NLGNAHIFLGRFDVAAEHYKK → QPYPTLDSVGSRLSTVAMIPQ
     272-673: Missing.

Note: No experimental confirmation available.
Show »
Length:303
Mass (Da):33,585
Checksum:iF989E9131BC88A20
GO
Isoform 5 (identifier: Q6IR34-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-22: MASPAPPVAEELPGPASRRLYS → MLASAMEGQP...VQKMRNLQRK

Show »
Length:705
Mass (Da):78,114
Checksum:iBCE1187E2231D151
GO

Sequence cautioni

The sequence AAH26486 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAH71197 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti668C → Y in AAB00119 (Ref. 6) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0390291 – 23Missing in isoform 3. CuratedAdd BLAST23
Alternative sequenceiVSP_0390301 – 22MASPA…RRLYS → MLASAMEGQPLALSLAEKAV CKVVYGAPRPRPLLLPVGLE LWLYVQKMRNLQRK in isoform 4 and isoform 5. 1 PublicationAdd BLAST22
Alternative sequenceiVSP_039031251 – 271NLGNA…EHYKK → QPYPTLDSVGSRLSTVAMIP Q in isoform 4. 1 PublicationAdd BLAST21
Alternative sequenceiVSP_039032272 – 673Missing in isoform 4. 1 PublicationAdd BLAST402
Alternative sequenceiVSP_039033424 – 483Missing in isoform 2. 1 PublicationAdd BLAST60

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK048691 mRNA. Translation: BAC33422.1.
AK084631 mRNA. Translation: BAC39236.1.
AL732541 Genomic DNA. Translation: CAM20327.1.
AL732541 Genomic DNA. Translation: CAM20328.1.
AL732541 Genomic DNA. Translation: CAM20329.1.
CH466542 Genomic DNA. Translation: EDL08301.1.
CH466542 Genomic DNA. Translation: EDL08300.1.
BC026486 mRNA. Translation: AAH26486.1. Different initiation.
BC071197 mRNA. Translation: AAH71197.1. Different initiation.
L23316 mRNA. Translation: AAB00119.1.
CCDSiCCDS15800.1. [Q6IR34-1]
CCDS57158.1. [Q6IR34-2]
CCDS57159.1. [Q6IR34-5]
RefSeqiNP_001186075.1. NM_001199146.1. [Q6IR34-2]
NP_001186076.1. NM_001199147.1. [Q6IR34-5]
NP_700459.2. NM_153410.5. [Q6IR34-1]
XP_006498316.1. XM_006498253.3. [Q6IR34-3]
UniGeneiMm.266611.

Genome annotation databases

EnsembliENSMUST00000066889; ENSMUSP00000067964; ENSMUSG00000026930. [Q6IR34-5]
ENSMUST00000066936; ENSMUSP00000065000; ENSMUSG00000026930. [Q6IR34-1]
ENSMUST00000078616; ENSMUSP00000077686; ENSMUSG00000026930. [Q6IR34-2]
GeneIDi67839.
KEGGimmu:67839.
UCSCiuc008iun.2. mouse. [Q6IR34-1]
uc008iuo.2. mouse. [Q6IR34-2]
uc008iup.2. mouse. [Q6IR34-5]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK048691 mRNA. Translation: BAC33422.1.
AK084631 mRNA. Translation: BAC39236.1.
AL732541 Genomic DNA. Translation: CAM20327.1.
AL732541 Genomic DNA. Translation: CAM20328.1.
AL732541 Genomic DNA. Translation: CAM20329.1.
CH466542 Genomic DNA. Translation: EDL08301.1.
CH466542 Genomic DNA. Translation: EDL08300.1.
BC026486 mRNA. Translation: AAH26486.1. Different initiation.
BC071197 mRNA. Translation: AAH71197.1. Different initiation.
L23316 mRNA. Translation: AAB00119.1.
CCDSiCCDS15800.1. [Q6IR34-1]
CCDS57158.1. [Q6IR34-2]
CCDS57159.1. [Q6IR34-5]
RefSeqiNP_001186075.1. NM_001199146.1. [Q6IR34-2]
NP_001186076.1. NM_001199147.1. [Q6IR34-5]
NP_700459.2. NM_153410.5. [Q6IR34-1]
XP_006498316.1. XM_006498253.3. [Q6IR34-3]
UniGeneiMm.266611.

3D structure databases

ProteinModelPortaliQ6IR34.
SMRiQ6IR34.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi212469. 11 interactors.
IntActiQ6IR34. 12 interactors.
MINTiMINT-8305267.
STRINGi10090.ENSMUSP00000067964.

PTM databases

iPTMnetiQ6IR34.
PhosphoSitePlusiQ6IR34.

Proteomic databases

PaxDbiQ6IR34.
PeptideAtlasiQ6IR34.
PRIDEiQ6IR34.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000066889; ENSMUSP00000067964; ENSMUSG00000026930. [Q6IR34-5]
ENSMUST00000066936; ENSMUSP00000065000; ENSMUSG00000026930. [Q6IR34-1]
ENSMUST00000078616; ENSMUSP00000077686; ENSMUSG00000026930. [Q6IR34-2]
GeneIDi67839.
KEGGimmu:67839.
UCSCiuc008iun.2. mouse. [Q6IR34-1]
uc008iuo.2. mouse. [Q6IR34-2]
uc008iup.2. mouse. [Q6IR34-5]

Organism-specific databases

CTDi26086.
MGIiMGI:1915089. Gpsm1.

Phylogenomic databases

eggNOGiKOG1130. Eukaryota.
ENOG410XP6N. LUCA.
GeneTreeiENSGT00530000063126.
HOVERGENiHBG051823.
InParanoidiQ6IR34.
KOiK15839.
OMAiQEPGDDF.
OrthoDBiEOG091G04C9.
PhylomeDBiQ6IR34.
TreeFamiTF328344.

Enzyme and pathway databases

ReactomeiR-MMU-418594. G alpha (i) signalling events.

Miscellaneous databases

ChiTaRSiGpsm1. mouse.
PROiQ6IR34.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026930.
ExpressionAtlasiQ6IR34. baseline and differential.
GenevisibleiQ6IR34. MM.

Family and domain databases

Gene3Di1.25.40.10. 2 hits.
InterProiIPR024804. G_prot_signal_mod_1.
IPR003109. GoLoco_motif.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR019734. TPR_repeat.
[Graphical view]
PANTHERiPTHR10098:SF36. PTHR10098:SF36. 1 hit.
PfamiPF02188. GoLoco. 4 hits.
[Graphical view]
SMARTiSM00390. GoLoco. 4 hits.
SM00028. TPR. 6 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 2 hits.
PROSITEiPS50877. GOLOCO. 4 hits.
PS50005. TPR. 6 hits.
PS50293. TPR_REGION. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGPSM1_MOUSE
AccessioniPrimary (citable) accession number: Q6IR34
Secondary accession number(s): A2AIX7
, A2AIX8, A2AIX9, Q61366, Q8BUK4, Q8BX78, Q8R0R9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 17, 2006
Last sequence update: April 20, 2010
Last modified: November 2, 2016
This is version 117 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.