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Protein

Chondroitin sulfate synthase 2

Gene

Chpf

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has both beta-1,3-glucuronic acid and beta-1,4-N-acetylgalactosamine transferase activity. Transfers glucuronic acid (GlcUA) from UDP-GlcUA and N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of the elongating chondroitin polymer (By similarity). Isoform 2 may facilitate PARK2 transport into the mitochondria. In collaboration with PARK2, isoform 2 may enhance cell viability and protect cells from oxidative stress (By similarity).By similarity

Catalytic activityi

UDP-N-acetyl-alpha-D-galactosamine + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-proteoglycan = UDP + N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-proteoglycan.
UDP-alpha-D-glucuronate + N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan = UDP + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan.

Cofactori

Mn2+By similarity, Co2+By similarityNote: Divalent cations. Highest activities are measured with manganese. Can also utilize cobalt.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi616 – 6161Divalent metal cationSequence analysis

GO - Molecular functioni

GO - Biological processi

  • chondroitin sulfate biosynthetic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-2022870. Chondroitin sulfate biosynthesis.

Protein family/group databases

CAZyiGT31. Glycosyltransferase Family 31.
GT7. Glycosyltransferase Family 7.

Names & Taxonomyi

Protein namesi
Recommended name:
Chondroitin sulfate synthase 2 (EC:2.4.1.175, EC:2.4.1.226)
Alternative name(s):
Chondroitin glucuronyltransferase 2
Chondroitin-polymerizing factor
Short name:
ChPF
Glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase II
N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase II
N-acetylgalactosaminyltransferase 2
Gene namesi
Name:Chpf
Synonyms:Css2, D1Bwg1363e
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:106576. Chpf.

Subcellular locationi

Isoform 1 :
Isoform 3 :
  • Cytoplasmcytosol By similarity
  • Mitochondrion By similarity
Isoform 2 :
  • Mitochondrion matrix By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1515CytoplasmicSequence analysisAdd
BLAST
Transmembranei16 – 3419Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini35 – 774740LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus, Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 774774Chondroitin sulfate synthase 2PRO_0000189561Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi138 – 1381N-linked (GlcNAc...)Sequence analysis
Glycosylationi361 – 3611N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiQ6IQX7.
MaxQBiQ6IQX7.
PaxDbiQ6IQX7.
PeptideAtlasiQ6IQX7.
PRIDEiQ6IQX7.

PTM databases

iPTMnetiQ6IQX7.
PhosphoSiteiQ6IQX7.

Expressioni

Tissue specificityi

Isoform 1, isoform 2 and isoform 3 are expressed in brain (at protein level).1 Publication

Gene expression databases

BgeeiQ6IQX7.
CleanExiMM_CHPF.
ExpressionAtlasiQ6IQX7. baseline and differential.
GenevisibleiQ6IQX7. MM.

Interactioni

Subunit structurei

Binds CHSY1. Isoform 1, isoform 2 and isoform 3 interact with PARK2.

Binary interactionsi

WithEntry#Exp.IntActNotes
Park2Q9WVS63EBI-9029659,EBI-973635

Protein-protein interaction databases

IntActiQ6IQX7. 1 interaction.
STRINGi10090.ENSMUSP00000078199.

Structurei

3D structure databases

ProteinModelPortaliQ6IQX7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3708. Eukaryota.
ENOG410XWZJ. LUCA.
GeneTreeiENSGT00760000119143.
HOGENOMiHOG000037934.
HOVERGENiHBG050941.
InParanoidiQ6IQX7.
KOiK00747.
OMAiCTGDHEG.
OrthoDBiEOG790G17.
PhylomeDBiQ6IQX7.
TreeFamiTF318303.

Family and domain databases

InterProiIPR008428. Chond_GalNAc.
[Graphical view]
PfamiPF05679. CHGN. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6IQX7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRASLLLSVL RPAGPVAVGI SLGFTLSLLS VTWVEEPCGP GPPQPGDSEL
60 70 80 90 100
PPRGNTNAAR RPNSVQPGSE RERPGAGAGT GESWEPRVLP YHPAQPGQAT
110 120 130 140 150
KKAVRTRYIS TELGIRQKLL VAVLTSQATL PTLGVAVNRT LGHRLEHVVF
160 170 180 190 200
LTGARGRRTP SGMAVVALGE ERPIGHLHLA LRHLLEQHGD DFDWFFLVPD
210 220 230 240 250
ATYTEAHGLD RLAGHLSLAS ATHLYLGRPQ DFIGGDTTPG RYCHGGFGVL
260 270 280 290 300
LSRTLLQQLR PHLESCRNDI VSARPDEWLG RCILDATGVG CTGDHEGMHY
310 320 330 340 350
NYLELSPGEP VQEGDPRFRS ALTAHPVRDP VHMYQLHKAF ARAELDRTYQ
360 370 380 390 400
EIQELQWEIQ NTSRLAADGE RASAWPVGIP APSRPASRFE VLRWDYFTEQ
410 420 430 440 450
YAFSCADGSP RCPLRGADQA DVADVLGTAL EELNRRYQPA LQLQKQQLVN
460 470 480 490 500
GYRRFDPARG MEYTLDLQLE ALTPQGGRWP LTRRVQLLRP LSRVEILPVP
510 520 530 540 550
YVTEASRLTV LLPLAAAERD LASGFLEAFA TAALEPGDAA ALTLLLLYEP
560 570 580 590 600
RQAQRAAHSD VFAPVKAHVA ELERRFPGAR VPWLSVQTAA PSPLRLMDLL
610 620 630 640 650
SKKHPLDTLF LLAGPDTVLT PDFLNRCRMH AISGWQAFFP MHFQAFHPAV
660 670 680 690 700
APPQGPGPPE LGRDTGHFDR QAASEACFYN SDYVAARGRL VAASEQEEEL
710 720 730 740 750
LESLDVYELF LRFSNLHVLR AVEPALLQRY RAQPCSARLS EDLYHRCRQS
760 770
VLEGLGSRTQ LAMLLFEQEQ GNST
Length:774
Mass (Da):85,534
Last modified:July 5, 2004 - v1
Checksum:iA3D3BA5E3142026B
GO
Isoform 2 (identifier: Q6IQX7-2) [UniParc]FASTAAdd to basket

Also known as: Klokin1

The sequence of this isoform differs from the canonical sequence as follows:
     1-460: Missing.

Show »
Length:314
Mass (Da):34,986
Checksum:i6286DDFB15717E29
GO
Isoform 3 (identifier: Q6IQX7-3) [UniParc]FASTAAdd to basket

Also known as: ChPF(D996)

The sequence of this isoform differs from the canonical sequence as follows:
     1-162: Missing.

Show »
Length:612
Mass (Da):68,446
Checksum:iC27AC3D4CE69CBB0
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 460460Missing in isoform 2. CuratedVSP_053435Add
BLAST
Alternative sequencei1 – 162162Missing in isoform 3. CuratedVSP_053436Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC115011 Genomic DNA. No translation available.
BC071273 mRNA. Translation: AAH71273.1.
BC057050 mRNA. No translation available.
BC060598 mRNA. No translation available.
CCDSiCCDS15074.1. [Q6IQX7-1]
CCDS15075.1. [Q6IQX7-3]
RefSeqiNP_001001565.1. NM_001001565.2. [Q6IQX7-3]
NP_001001566.1. NM_001001566.3. [Q6IQX7-1]
UniGeneiMm.260577.

Genome annotation databases

EnsembliENSMUST00000079205; ENSMUSP00000078199; ENSMUSG00000032997. [Q6IQX7-1]
ENSMUST00000094818; ENSMUSP00000092412; ENSMUSG00000032997. [Q6IQX7-3]
GeneIDi74241.
KEGGimmu:74241.
UCSCiuc007bph.1. mouse. [Q6IQX7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC115011 Genomic DNA. No translation available.
BC071273 mRNA. Translation: AAH71273.1.
BC057050 mRNA. No translation available.
BC060598 mRNA. No translation available.
CCDSiCCDS15074.1. [Q6IQX7-1]
CCDS15075.1. [Q6IQX7-3]
RefSeqiNP_001001565.1. NM_001001565.2. [Q6IQX7-3]
NP_001001566.1. NM_001001566.3. [Q6IQX7-1]
UniGeneiMm.260577.

3D structure databases

ProteinModelPortaliQ6IQX7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ6IQX7. 1 interaction.
STRINGi10090.ENSMUSP00000078199.

Protein family/group databases

CAZyiGT31. Glycosyltransferase Family 31.
GT7. Glycosyltransferase Family 7.

PTM databases

iPTMnetiQ6IQX7.
PhosphoSiteiQ6IQX7.

Proteomic databases

EPDiQ6IQX7.
MaxQBiQ6IQX7.
PaxDbiQ6IQX7.
PeptideAtlasiQ6IQX7.
PRIDEiQ6IQX7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000079205; ENSMUSP00000078199; ENSMUSG00000032997. [Q6IQX7-1]
ENSMUST00000094818; ENSMUSP00000092412; ENSMUSG00000032997. [Q6IQX7-3]
GeneIDi74241.
KEGGimmu:74241.
UCSCiuc007bph.1. mouse. [Q6IQX7-1]

Organism-specific databases

CTDi79586.
MGIiMGI:106576. Chpf.

Phylogenomic databases

eggNOGiKOG3708. Eukaryota.
ENOG410XWZJ. LUCA.
GeneTreeiENSGT00760000119143.
HOGENOMiHOG000037934.
HOVERGENiHBG050941.
InParanoidiQ6IQX7.
KOiK00747.
OMAiCTGDHEG.
OrthoDBiEOG790G17.
PhylomeDBiQ6IQX7.
TreeFamiTF318303.

Enzyme and pathway databases

ReactomeiR-MMU-2022870. Chondroitin sulfate biosynthesis.

Miscellaneous databases

PROiQ6IQX7.
SOURCEiSearch...

Gene expression databases

BgeeiQ6IQX7.
CleanExiMM_CHPF.
ExpressionAtlasiQ6IQX7. baseline and differential.
GenevisibleiQ6IQX7. MM.

Family and domain databases

InterProiIPR008428. Chond_GalNAc.
[Graphical view]
PfamiPF05679. CHGN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Embryo.
  3. "Parkin interacts with Klokin1 for mitochondrial import and maintenance of membrane potential."
    Kuroda Y., Sako W., Goto S., Sawada T., Uchida D., Izumi Y., Takahashi T., Kagawa N., Matsumoto M., Matsumoto M., Takahashi R., Kaji R., Mitsui T.
    Hum. Mol. Genet. 21:991-1003(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: ALTERNATIVE SPLICING (ISOFORMS 2 AND 3), INTERACTION WITH PARK2, TISSUE SPECIFICITY.

Entry informationi

Entry nameiCHSS2_MOUSE
AccessioniPrimary (citable) accession number: Q6IQX7
Secondary accession number(s): E9PUB9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: July 5, 2004
Last modified: July 6, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.