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Protein

DENN domain-containing protein 5A

Gene

DENND5A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Guanine nucleotide exchange factor (GEF) which may activate RAB6A and RAB39A and/or RAB39B. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form.1 Publication

GO - Molecular functioni

  • calcium channel activity Source: GO_Central
  • Rab guanyl-nucleotide exchange factor activity Source: UniProtKB

GO - Biological processi

  • calcium ion transmembrane transport Source: GOC
  • detection of mechanical stimulus Source: GO_Central
  • positive regulation of GTPase activity Source: GOC
  • retrograde transport, endosome to Golgi Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Names & Taxonomyi

Protein namesi
Recommended name:
DENN domain-containing protein 5A
Alternative name(s):
Rab6-interacting protein 1
Short name:
Rab6IP1
Gene namesi
Name:DENND5A
Synonyms:KIAA1091, RAB6IP1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:19344. DENND5A.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: GOC
  • trans-Golgi network Source: UniProtKB
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA164718737.

Polymorphism and mutation databases

BioMutaiDENND5A.
DMDMi317373354.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12871287DENN domain-containing protein 5APRO_0000097142Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei193 – 1931PhosphoserineCombined sources
Modified residuei1079 – 10791PhosphothreonineBy similarity
Modified residuei1085 – 10851PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ6IQ26.
PaxDbiQ6IQ26.
PRIDEiQ6IQ26.

PTM databases

iPTMnetiQ6IQ26.
PhosphoSiteiQ6IQ26.

Expressioni

Gene expression databases

BgeeiQ6IQ26.
CleanExiHS_DENND5A.
ExpressionAtlasiQ6IQ26. baseline and differential.
GenevisibleiQ6IQ26. HS.

Organism-specific databases

HPAiHPA052923.
HPA055009.

Interactioni

Subunit structurei

Probably interacts with RAB6A bound to GTP.By similarity

Protein-protein interaction databases

BioGridi116861. 5 interactions.
IntActiQ6IQ26. 3 interactions.
MINTiMINT-8247429.
STRINGi9606.ENSP00000328524.

Structurei

3D structure databases

ProteinModelPortaliQ6IQ26.
SMRiQ6IQ26. Positions 286-405, 737-1072.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini68 – 13871UDENNAdd
BLAST
Domaini202 – 390189DENNPROSITE-ProRule annotationAdd
BLAST
Domaini512 – 58877dDENNPROSITE-ProRule annotationAdd
BLAST
Domaini787 – 950164RUN 1PROSITE-ProRule annotationAdd
BLAST
Domaini954 – 1062109PLATPROSITE-ProRule annotationAdd
BLAST
Domaini1134 – 1282149RUN 2PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi3 – 97Poly-Gly
Compositional biasi593 – 5986Poly-Asp

Sequence similaritiesi

Belongs to the RAB6IP1 family.Curated
Contains 1 dDENN domain.PROSITE-ProRule annotation
Contains 1 DENN domain.PROSITE-ProRule annotation
Contains 1 PLAT domain.PROSITE-ProRule annotation
Contains 2 RUN domains.PROSITE-ProRule annotation
Contains 1 uDENN domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2080. Eukaryota.
ENOG410XNYP. LUCA.
GeneTreeiENSGT00760000118819.
HOGENOMiHOG000019389.
HOVERGENiHBG059906.
InParanoidiQ6IQ26.
OMAiAKWLVEC.
OrthoDBiEOG7FJGZP.
PhylomeDBiQ6IQ26.
TreeFamiTF313237.

Family and domain databases

Gene3Di2.60.60.20. 1 hit.
InterProiIPR005112. dDENN_dom.
IPR001194. DENN_dom.
IPR001024. PLAT/LH2_dom.
IPR004012. Run_dom.
IPR005113. uDENN_dom.
[Graphical view]
PfamiPF03455. dDENN. 1 hit.
PF02141. DENN. 1 hit.
PF01477. PLAT. 1 hit.
PF02759. RUN. 2 hits.
PF03456. uDENN. 1 hit.
[Graphical view]
SMARTiSM00801. dDENN. 1 hit.
SM00799. DENN. 1 hit.
SM00593. RUN. 2 hits.
SM00800. uDENN. 1 hit.
[Graphical view]
SUPFAMiSSF49723. SSF49723. 1 hit.
PROSITEiPS50947. DDENN. 1 hit.
PS50211. DENN. 1 hit.
PS50095. PLAT. 1 hit.
PS50826. RUN. 2 hits.
PS50946. UDENN. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6IQ26-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSGGGGGGGS APSRFADYFV ICGLDTETGL EPDELSALCQ YIQASKARDG
60 70 80 90 100
ASPFISSTTE GENFEQTPLR RTFKSKVLAR YPENVEWNPF DQDAVGMLCM
110 120 130 140 150
PKGLAFKTQA DPREPQFHAF IITREDGSRT FGFALTFYEE VTSKQICSAM
160 170 180 190 200
QTLYHMHNAE YDVLHAPPAD DRDQSSMEDG EDTPVTKLQR FNSYDISRDT
210 220 230 240 250
LYVSKCICLI TPMSFMKACR SVLEQLHQAV TSPQPPPLPL ESYIYNVLYE
260 270 280 290 300
VPLPPPGRSL KFSGVYGPII CQRPSTNELP LFDFPVKEVF ELLGVENVFQ
310 320 330 340 350
LFTCALLEFQ ILLYSQHYQR LMTVAETITA LMFPFQWQHV YVPILPASLL
360 370 380 390 400
HFLDAPVPYL MGLHSNGLDD RSKLELPQEA NLCFVDIDNH FIELPEDLPQ
410 420 430 440 450
FPNKLEFVQE VSEILMAFGI PPEGNLHCSE SASKLKRLRA SELVSDKRNG
460 470 480 490 500
NIAGSPLHSY ELLKENETIA RLQALVKRTG VSLEKLEVRE DPSSNKDLKV
510 520 530 540 550
QCDEEELRIY QLNIQIREVF ANRFTQMFAD YEVFVIQPSQ DKESWFTNRE
560 570 580 590 600
QMQNFDKASF LSDQPEPYLP FLSRFLETQM FASFIDNKIM CHDDDDKDPV
610 620 630 640 650
LRVFDSRVDK IRLLNVRTPT LRTSMYQKCT TVDEAEKAIE LRLAKIDHTA
660 670 680 690 700
IHPHLLDMKI GQGKYEPGFF PKLQSDVLST GPASNKWTKR NAPAQWRRKD
710 720 730 740 750
RQKQHTEHLR LDNDQREKYI QEARTMGSTI RQPKLSNLSP SVIAQTNWKF
760 770 780 790 800
VEGLLKECRN KTKRMLVEKM GREAVELGHG EVNITGVEEN TLIASLCDLL
810 820 830 840 850
ERIWSHGLQV KQGKSALWSH LLHYQDNRQR KLTSGSLSTS GILLDSERRK
860 870 880 890 900
SDASSLMPPL RISLIQDMRH IQNIGEIKTD VGKARAWVRL SMEKKLLSRH
910 920 930 940 950
LKQLLSDHEL TKKLYKRYAF LRCDDEKEQF LYHLLSFNAV DYFCFTNVFT
960 970 980 990 1000
TILIPYHILI VPSKKLGGSM FTANPWICIS GELGETQIMQ IPRNVLEMTF
1010 1020 1030 1040 1050
ECQNLGKLTT VQIGHDNSGL YAKWLVEYVM VRNEITGHTY KFPCGRWLGK
1060 1070 1080 1090 1100
GMDDGSLERI LVGELLTSQP EVDERPCRTP PLQQSPSVIR RLVTISPNNK
1110 1120 1130 1140 1150
PKLNTGQIQE SIGEAVNGIV KHFHKPEKER GSLTLLLCGE CGLVSALEQA
1160 1170 1180 1190 1200
FQHGFKSPRL FKNVFIWDFL EKAQTYYETL EKNEVVPEEN WHTRARNFCR
1210 1220 1230 1240 1250
FVTAINNTPR NIGKDGKFQM LVCLGARDHL LHHWIALLAD CPITAHMYED
1260 1270 1280
VALIKDHTLV NSLIRVLQTL QEFNITLETS LVKGIDI
Length:1,287
Mass (Da):147,096
Last modified:January 11, 2011 - v2
Checksum:i7633D36BE076C576
GO
Isoform 2 (identifier: Q6IQ26-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1216-1287: GKFQMLVCLG...ETSLVKGIDI → EITSYTTGLPCWLTAPSLHTCMRMWH

Note: No experimental confirmation available.
Show »
Length:1,241
Mass (Da):141,982
Checksum:iB6767A498123DC4E
GO

Sequence cautioni

The sequence BAA83043.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BAB15155.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti597 – 5971K → E in BAB15155 (PubMed:14702039).Curated
Sequence conflicti929 – 9291Q → R in BAG58677 (PubMed:14702039).Curated
Sequence conflicti998 – 9981M → V in BAG58677 (PubMed:14702039).Curated
Sequence conflicti1100 – 11001K → E in BAB15155 (PubMed:14702039).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti224 – 2241E → Q.3 Publications
Corresponds to variant rs952374 [ dbSNP | Ensembl ].
VAR_028409

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1216 – 128772GKFQM…KGIDI → EITSYTTGLPCWLTAPSLHT CMRMWH in isoform 2. 1 PublicationVSP_044688Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB029014 mRNA. Translation: BAA83043.1. Different initiation.
AK025499 mRNA. Translation: BAB15155.1. Different initiation.
AK295881 mRNA. Translation: BAG58677.1.
AL117448 mRNA. Translation: CAB55932.2.
AC055845 Genomic DNA. No translation available.
AC079296 Genomic DNA. No translation available.
AP006259 Genomic DNA. No translation available.
BC009354 mRNA. Translation: AAH09354.2.
BC071596 mRNA. Translation: AAH71596.1.
CCDSiCCDS31423.1. [Q6IQ26-1]
CCDS58119.1. [Q6IQ26-2]
PIRiT17242.
RefSeqiNP_001230183.1. NM_001243254.1. [Q6IQ26-2]
NP_056028.2. NM_015213.3. [Q6IQ26-1]
UniGeneiHs.501857.

Genome annotation databases

EnsembliENST00000328194; ENSP00000328524; ENSG00000184014. [Q6IQ26-1]
ENST00000530044; ENSP00000435866; ENSG00000184014. [Q6IQ26-2]
GeneIDi23258.
KEGGihsa:23258.
UCSCiuc001mhl.5. human. [Q6IQ26-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB029014 mRNA. Translation: BAA83043.1. Different initiation.
AK025499 mRNA. Translation: BAB15155.1. Different initiation.
AK295881 mRNA. Translation: BAG58677.1.
AL117448 mRNA. Translation: CAB55932.2.
AC055845 Genomic DNA. No translation available.
AC079296 Genomic DNA. No translation available.
AP006259 Genomic DNA. No translation available.
BC009354 mRNA. Translation: AAH09354.2.
BC071596 mRNA. Translation: AAH71596.1.
CCDSiCCDS31423.1. [Q6IQ26-1]
CCDS58119.1. [Q6IQ26-2]
PIRiT17242.
RefSeqiNP_001230183.1. NM_001243254.1. [Q6IQ26-2]
NP_056028.2. NM_015213.3. [Q6IQ26-1]
UniGeneiHs.501857.

3D structure databases

ProteinModelPortaliQ6IQ26.
SMRiQ6IQ26. Positions 286-405, 737-1072.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116861. 5 interactions.
IntActiQ6IQ26. 3 interactions.
MINTiMINT-8247429.
STRINGi9606.ENSP00000328524.

PTM databases

iPTMnetiQ6IQ26.
PhosphoSiteiQ6IQ26.

Polymorphism and mutation databases

BioMutaiDENND5A.
DMDMi317373354.

Proteomic databases

MaxQBiQ6IQ26.
PaxDbiQ6IQ26.
PRIDEiQ6IQ26.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000328194; ENSP00000328524; ENSG00000184014. [Q6IQ26-1]
ENST00000530044; ENSP00000435866; ENSG00000184014. [Q6IQ26-2]
GeneIDi23258.
KEGGihsa:23258.
UCSCiuc001mhl.5. human. [Q6IQ26-1]

Organism-specific databases

CTDi23258.
GeneCardsiDENND5A.
H-InvDBHIX0009434.
HGNCiHGNC:19344. DENND5A.
HPAiHPA052923.
HPA055009.
neXtProtiNX_Q6IQ26.
PharmGKBiPA164718737.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2080. Eukaryota.
ENOG410XNYP. LUCA.
GeneTreeiENSGT00760000118819.
HOGENOMiHOG000019389.
HOVERGENiHBG059906.
InParanoidiQ6IQ26.
OMAiAKWLVEC.
OrthoDBiEOG7FJGZP.
PhylomeDBiQ6IQ26.
TreeFamiTF313237.

Miscellaneous databases

ChiTaRSiDENND5A. human.
GenomeRNAii23258.
PROiQ6IQ26.

Gene expression databases

BgeeiQ6IQ26.
CleanExiHS_DENND5A.
ExpressionAtlasiQ6IQ26. baseline and differential.
GenevisibleiQ6IQ26. HS.

Family and domain databases

Gene3Di2.60.60.20. 1 hit.
InterProiIPR005112. dDENN_dom.
IPR001194. DENN_dom.
IPR001024. PLAT/LH2_dom.
IPR004012. Run_dom.
IPR005113. uDENN_dom.
[Graphical view]
PfamiPF03455. dDENN. 1 hit.
PF02141. DENN. 1 hit.
PF01477. PLAT. 1 hit.
PF02759. RUN. 2 hits.
PF03456. uDENN. 1 hit.
[Graphical view]
SMARTiSM00801. dDENN. 1 hit.
SM00799. DENN. 1 hit.
SM00593. RUN. 2 hits.
SM00800. uDENN. 1 hit.
[Graphical view]
SUPFAMiSSF49723. SSF49723. 1 hit.
PROSITEiPS50947. DDENN. 1 hit.
PS50211. DENN. 1 hit.
PS50095. PLAT. 1 hit.
PS50826. RUN. 2 hits.
PS50946. UDENN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Prediction of the coding sequences of unidentified human genes. XIV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
    Kikuno R., Nagase T., Ishikawa K., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
    DNA Res. 6:197-205(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT GLN-224.
    Tissue: Brain.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 379-1287 (ISOFORM 1), VARIANT GLN-224.
    Tissue: Hippocampus.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Uterus.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT GLN-224.
    Tissue: Placenta and Skin.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-193, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  7. "Family-wide characterization of the DENN domain Rab GDP-GTP exchange factors."
    Yoshimura S., Gerondopoulos A., Linford A., Rigden D.J., Barr F.A.
    J. Cell Biol. 191:367-381(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS GUANYL-NUCLEOTIDE EXCHANGE FACTOR.

Entry informationi

Entry nameiDEN5A_HUMAN
AccessioniPrimary (citable) accession number: Q6IQ26
Secondary accession number(s): B4DJ15
, E9PS91, Q96GN3, Q9H6U7, Q9UFV0, Q9UPR1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2004
Last sequence update: January 11, 2011
Last modified: June 8, 2016
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.