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Q6IQ23 (PKHA7_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pleckstrin homology domain-containing family A member 7

Short name=PH domain-containing family A member 7
Gene names
Name:PLEKHA7
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1121 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for zonula adherens biogenesis and maintenance. Acts via its interaction with KIAA1543/Nezha, which anchors microtubules at their minus-ends to zonula adherens, leading to the recruitment of KIFC3 kinesin to the junctional site. Ref.5

Subunit structure

Interacts with KIAA1543/Nezha and CTNND1. Ref.5

Subcellular location

Cell junctionadherens junction. Cytoplasm. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome. Note: Localizes to zonula adherens, recruited via its interaction with CTNND1. Ref.5

Sequence similarities

Contains 1 PH domain.

Contains 2 WW domains.

Sequence caution

The sequence AAH33239.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence BAG62985.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q6IQ23-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q6IQ23-2)

The sequence of this isoform differs from the canonical sequence as follows:
     915-915: V → VQ
Isoform 3 (identifier: Q6IQ23-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-447: MAAATVGRDT...WARAQKGDSR → MFPKACRTLAWLPDPFLPFLL
     915-915: V → VQ
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11211121Pleckstrin homology domain-containing family A member 7
PRO_0000287692

Regions

Domain9 – 4234WW 1
Domain54 – 8734WW 2
Domain164 – 282119PH
Region538 – 696159Interaction with CTNND1
Coiled coil700 – 801102 Potential
Coiled coil1067 – 109428 Potential
Compositional bias567 – 59933Pro-rich
Compositional bias845 – 94298Pro-rich

Amino acid modifications

Modified residue5361Phosphoserine By similarity
Modified residue5451Phosphoserine By similarity
Modified residue6041Phosphoserine Ref.8
Modified residue6121Phosphoserine Ref.8
Modified residue9031Phosphoserine Ref.6 Ref.8 Ref.9
Modified residue9071Phosphoserine Ref.6 Ref.8 Ref.9
Modified residue9861Phosphoserine Ref.8

Natural variations

Alternative sequence1 – 447447MAAAT…KGDSR → MFPKACRTLAWLPDPFLPFL L in isoform 3.
VSP_039543
Alternative sequence9151V → VQ in isoform 2 and isoform 3.
VSP_025592
Natural variant2411L → I.
Corresponds to variant rs35908144 [ dbSNP | Ensembl ].
VAR_032346
Natural variant2481A → V.
Corresponds to variant rs16933529 [ dbSNP | Ensembl ].
VAR_032347
Natural variant2791Q → R.
Corresponds to variant rs369819 [ dbSNP | Ensembl ].
VAR_032348
Natural variant6891S → R.
Corresponds to variant rs61133161 [ dbSNP | Ensembl ].
VAR_061517
Natural variant6931V → I.
Corresponds to variant rs34556458 [ dbSNP | Ensembl ].
VAR_032349

Experimental info

Sequence conflict4941D → N in BAG62985. Ref.1
Sequence conflict9811L → P in AAH71599. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 25, 2008. Version 2.
Checksum: 93F616CF3EF9E5C2

FASTA1,121127,135
        10         20         30         40         50         60 
MAAATVGRDT LPEHWSYGVC RDGRVFFIND QLRCTTWLHP RTGEPVNSGH MIRSDLPRGW 

        70         80         90        100        110        120 
EEGFTEEGAS YFIDHNQQTT AFRHPVTGQF SPENSEFILQ EEPNPHMSKQ DRNQRPSSMV 

       130        140        150        160        170        180 
SETSTAGTAS TLEAKPGPKI IKSSSKVHSF GKRDQAIRRN PNVPVVVRGW LHKQDSSGMR 

       190        200        210        220        230        240 
LWKRRWFVLA DYCLFYYKDS REEAVLGSIP LPSYVISPVA PEDRISRKYS FKAVHTGMRA 

       250        260        270        280        290        300 
LIYNSSTAGS QAEQSGMRTY YFSADTQEDM NAWVRAMNQA AQVLSRSSLK RDMEKVERQA 

       310        320        330        340        350        360 
VPQANHTESC HECGRVGPGH TRDCPHRGHD DIVNFERQEQ EGEQYRSQRD PLEGKRDRSK 

       370        380        390        400        410        420 
ARSPYSPAEE DALFMDLPTG PRGQQAQPQR AEKNGMLPAS YGPGEQNGTG GYQRAFPPRT 

       430        440        450        460        470        480 
NPEKHSQRKS NLAQVEHWAR AQKGDSRSLP LDQTLPRQGP GQSLSFPENY QTLPKSTRHP 

       490        500        510        520        530        540 
SGGSSPPPRN LPSDYKYAQD RASHLKMSSE ERRAHRDGTV WQLYEWQQRQ QFRHGSPTAP 

       550        560        570        580        590        600 
ICLGSPEFTD QGRSRSMLEV PRSISVPPSP SDIPPPGPPR VFPPRRPHTP AERVTVKPPD 

       610        620        630        640        650        660 
QRRSVDISLG DSPRRARGHA VKNSSHVDRR SMPSMGYMTH TVSAPSLHGK SADDTYLQLK 

       670        680        690        700        710        720 
KDLEYLDLKM TGRDLLKDRS LKPVKIAESD TDVKLSIFCE QDRVLQDLED KIRALKENKD 

       730        740        750        760        770        780 
QLESVLEVLH RQMEQYRDQP QHLEKIAYQQ KLLQEDLVHI RAELSRESTE MENAWNEYLK 

       790        800        810        820        830        840 
LENDVEQLKQ TLQEQHRRAF FFQEKSQIQK DLWRIEDVTA GLSANKENFR ILVESVKNPE 

       850        860        870        880        890        900 
RKTVPLFPHP PVPSLSTSES KPPPQPSPPT SPVRTPLEVR LFPQLQTYVP YRPHPPQLRK 

       910        920        930        940        950        960 
VTSPLQSPTK AKPKVEDEAP PRPPLPELYS PEDQPPAVPP LPREATIIRH TSVRGLKRQS 

       970        980        990       1000       1010       1020 
DERKRDRELG QCVNGDSRVE LRSYVSEPEL ATLSGDMAQP SLGLVGPESR YQTLPGRGLS 

      1030       1040       1050       1060       1070       1080 
GSTSRLQQSS TIAPYVTLRR GLNAESSKAT FPRPKSALER LYSGDHQRGK MSAEEQLERM 

      1090       1100       1110       1120 
KRHQKALVRE RKRTLGQGER TGLPSSRYLS RPLPGDLGSV C 

« Hide

Isoform 2 [UniParc].

Checksum: 9E8C9F36A22A030B
Show »

FASTA1,122127,263
Isoform 3 [UniParc].

Checksum: 2E0584D8332267A3
Show »

FASTA69679,104

References

« Hide 'large scale' references
[1]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
Tissue: Synovium and Thalamus.
[2]"Human chromosome 11 DNA sequence and analysis including novel gene identification."
Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. expand/collapse author list , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y.
Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Brain and Placenta.
[4]"Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry."
Brill L.M., Salomon A.R., Ficarro S.B., Mukherji M., Stettler-Gill M., Peters E.C.
Anal. Chem. 76:2763-2772(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
[5]"Anchorage of microtubule minus ends to adherens junctions regulates epithelial cell-cell contacts."
Meng W., Mushika Y., Ichii T., Takeichi M.
Cell 135:948-959(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH KIAA1543 AND CTNND1.
[6]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-903 AND SER-907, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[7]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-604; SER-612; SER-903; SER-907 AND SER-986, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
[9]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-903 AND SER-907, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK296371 mRNA. Translation: BAG59045.1.
AK301465 mRNA. Translation: BAG62985.1. Different initiation.
AC026639 Genomic DNA. No translation available.
AC116533 Genomic DNA. No translation available.
AC127033 Genomic DNA. No translation available.
BC033239 mRNA. Translation: AAH33239.1. Different initiation.
BC071599 mRNA. Translation: AAH71599.1.
RefSeqNP_778228.3. NM_175058.4.
UniGeneHs.12332.

3D structure databases

ProteinModelPortalQ6IQ23.
SMRQ6IQ23. Positions 9-88, 157-282.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid126828. 5 interactions.
IntActQ6IQ23. 3 interactions.
STRING9606.ENSP00000347883.

PTM databases

PhosphoSiteQ6IQ23.

Polymorphism databases

DMDM215273867.

Proteomic databases

PaxDbQ6IQ23.
PRIDEQ6IQ23.

Protocols and materials databases

DNASU144100.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000355661; ENSP00000347883; ENSG00000166689. [Q6IQ23-1]
ENST00000448080; ENSP00000416895; ENSG00000166689. [Q6IQ23-2]
GeneID144100.
KEGGhsa:144100.
UCSCuc001mmn.3. human. [Q6IQ23-2]
uc001mmo.3. human. [Q6IQ23-1]
uc010rcv.2. human. [Q6IQ23-3]

Organism-specific databases

CTD144100.
GeneCardsGC11M016809.
HGNCHGNC:27049. PLEKHA7.
HPAHPA038610.
MIM612686. gene.
neXtProtNX_Q6IQ23.
PharmGKBPA134894945.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG309520.
HOGENOMHOG000077378.
HOVERGENHBG103572.
InParanoidQ6IQ23.
PhylomeDBQ6IQ23.
TreeFamTF329090.

Gene expression databases

ArrayExpressQ6IQ23.
BgeeQ6IQ23.
CleanExHS_PLEKHA7.
GenevestigatorQ6IQ23.

Family and domain databases

Gene3D2.30.29.30. 1 hit.
InterProIPR011993. PH_like_dom.
IPR001849. Pleckstrin_homology.
IPR001202. WW_dom.
[Graphical view]
PfamPF00169. PH. 1 hit.
[Graphical view]
SMARTSM00233. PH. 1 hit.
SM00456. WW. 2 hits.
[Graphical view]
SUPFAMSSF51045. SSF51045. 2 hits.
PROSITEPS50003. PH_DOMAIN. 1 hit.
PS01159. WW_DOMAIN_1. 1 hit.
PS50020. WW_DOMAIN_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSPLEKHA7. human.
GenomeRNAi144100.
NextBio84824.
PROQ6IQ23.
SOURCESearch...

Entry information

Entry namePKHA7_HUMAN
AccessionPrimary (citable) accession number: Q6IQ23
Secondary accession number(s): B4DK33, B4DWC3, Q86VZ7
Entry history
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: November 25, 2008
Last modified: April 16, 2014
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM