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Q6IQ23

- PKHA7_HUMAN

UniProt

Q6IQ23 - PKHA7_HUMAN

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Protein

Pleckstrin homology domain-containing family A member 7

Gene

PLEKHA7

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Required for zonula adherens biogenesis and maintenance. Acts via its interaction with KIAA1543/Nezha, which anchors microtubules at their minus-ends to zonula adherens, leading to the recruitment of KIFC3 kinesin to the junctional site.1 Publication

GO - Molecular functioni

  1. delta-catenin binding Source: UniProtKB

GO - Biological processi

  1. epithelial cell-cell adhesion Source: UniProtKB
  2. zonula adherens maintenance Source: UniProtKB
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Pleckstrin homology domain-containing family A member 7
Short name:
PH domain-containing family A member 7
Gene namesi
Name:PLEKHA7
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 11

Organism-specific databases

HGNCiHGNC:27049. PLEKHA7.

Subcellular locationi

Cell junctionadherens junction 1 Publication. Cytoplasm 1 Publication. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome 1 Publication
Note: Localizes to zonula adherens, recruited via its interaction with CTNND1.

GO - Cellular componenti

  1. centrosome Source: UniProtKB
  2. cytoplasm Source: UniProtKB-KW
  3. extracellular vesicular exosome Source: UniProt
  4. zonula adherens Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasm, Cytoskeleton

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134894945.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11211121Pleckstrin homology domain-containing family A member 7PRO_0000287692Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei536 – 5361PhosphoserineBy similarity
Modified residuei545 – 5451PhosphoserineBy similarity
Modified residuei604 – 6041Phosphoserine1 Publication
Modified residuei612 – 6121Phosphoserine1 Publication
Modified residuei903 – 9031Phosphoserine3 Publications
Modified residuei907 – 9071Phosphoserine3 Publications
Modified residuei986 – 9861Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ6IQ23.
PaxDbiQ6IQ23.
PRIDEiQ6IQ23.

PTM databases

PhosphoSiteiQ6IQ23.

Expressioni

Gene expression databases

BgeeiQ6IQ23.
CleanExiHS_PLEKHA7.
ExpressionAtlasiQ6IQ23. baseline and differential.
GenevestigatoriQ6IQ23.

Organism-specific databases

HPAiHPA038610.

Interactioni

Subunit structurei

Interacts with KIAA1543/Nezha and CTNND1.1 Publication

Protein-protein interaction databases

BioGridi126828. 7 interactions.
IntActiQ6IQ23. 3 interactions.
STRINGi9606.ENSP00000347883.

Structurei

3D structure databases

ProteinModelPortaliQ6IQ23.
SMRiQ6IQ23. Positions 11-85, 157-282.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini9 – 4234WW 1PROSITE-ProRule annotationAdd
BLAST
Domaini54 – 8734WW 2PROSITE-ProRule annotationAdd
BLAST
Domaini164 – 282119PHPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni538 – 696159Interaction with CTNND1Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili700 – 801102Sequence AnalysisAdd
BLAST
Coiled coili1067 – 109428Sequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi567 – 59933Pro-richAdd
BLAST
Compositional biasi845 – 94298Pro-richAdd
BLAST

Sequence similaritiesi

Contains 1 PH domain.PROSITE-ProRule annotation
Contains 2 WW domains.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiNOG309520.
GeneTreeiENSGT00530000063012.
HOGENOMiHOG000077378.
HOVERGENiHBG103572.
InParanoidiQ6IQ23.
PhylomeDBiQ6IQ23.
TreeFamiTF329090.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR001202. WW_dom.
[Graphical view]
PfamiPF00169. PH. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00456. WW. 2 hits.
[Graphical view]
SUPFAMiSSF51045. SSF51045. 2 hits.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS01159. WW_DOMAIN_1. 1 hit.
PS50020. WW_DOMAIN_2. 2 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q6IQ23-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAATVGRDT LPEHWSYGVC RDGRVFFIND QLRCTTWLHP RTGEPVNSGH
60 70 80 90 100
MIRSDLPRGW EEGFTEEGAS YFIDHNQQTT AFRHPVTGQF SPENSEFILQ
110 120 130 140 150
EEPNPHMSKQ DRNQRPSSMV SETSTAGTAS TLEAKPGPKI IKSSSKVHSF
160 170 180 190 200
GKRDQAIRRN PNVPVVVRGW LHKQDSSGMR LWKRRWFVLA DYCLFYYKDS
210 220 230 240 250
REEAVLGSIP LPSYVISPVA PEDRISRKYS FKAVHTGMRA LIYNSSTAGS
260 270 280 290 300
QAEQSGMRTY YFSADTQEDM NAWVRAMNQA AQVLSRSSLK RDMEKVERQA
310 320 330 340 350
VPQANHTESC HECGRVGPGH TRDCPHRGHD DIVNFERQEQ EGEQYRSQRD
360 370 380 390 400
PLEGKRDRSK ARSPYSPAEE DALFMDLPTG PRGQQAQPQR AEKNGMLPAS
410 420 430 440 450
YGPGEQNGTG GYQRAFPPRT NPEKHSQRKS NLAQVEHWAR AQKGDSRSLP
460 470 480 490 500
LDQTLPRQGP GQSLSFPENY QTLPKSTRHP SGGSSPPPRN LPSDYKYAQD
510 520 530 540 550
RASHLKMSSE ERRAHRDGTV WQLYEWQQRQ QFRHGSPTAP ICLGSPEFTD
560 570 580 590 600
QGRSRSMLEV PRSISVPPSP SDIPPPGPPR VFPPRRPHTP AERVTVKPPD
610 620 630 640 650
QRRSVDISLG DSPRRARGHA VKNSSHVDRR SMPSMGYMTH TVSAPSLHGK
660 670 680 690 700
SADDTYLQLK KDLEYLDLKM TGRDLLKDRS LKPVKIAESD TDVKLSIFCE
710 720 730 740 750
QDRVLQDLED KIRALKENKD QLESVLEVLH RQMEQYRDQP QHLEKIAYQQ
760 770 780 790 800
KLLQEDLVHI RAELSRESTE MENAWNEYLK LENDVEQLKQ TLQEQHRRAF
810 820 830 840 850
FFQEKSQIQK DLWRIEDVTA GLSANKENFR ILVESVKNPE RKTVPLFPHP
860 870 880 890 900
PVPSLSTSES KPPPQPSPPT SPVRTPLEVR LFPQLQTYVP YRPHPPQLRK
910 920 930 940 950
VTSPLQSPTK AKPKVEDEAP PRPPLPELYS PEDQPPAVPP LPREATIIRH
960 970 980 990 1000
TSVRGLKRQS DERKRDRELG QCVNGDSRVE LRSYVSEPEL ATLSGDMAQP
1010 1020 1030 1040 1050
SLGLVGPESR YQTLPGRGLS GSTSRLQQSS TIAPYVTLRR GLNAESSKAT
1060 1070 1080 1090 1100
FPRPKSALER LYSGDHQRGK MSAEEQLERM KRHQKALVRE RKRTLGQGER
1110 1120
TGLPSSRYLS RPLPGDLGSV C
Length:1,121
Mass (Da):127,135
Last modified:November 25, 2008 - v2
Checksum:i93F616CF3EF9E5C2
GO
Isoform 2 (identifier: Q6IQ23-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     915-915: V → VQ

Show »
Length:1,122
Mass (Da):127,263
Checksum:i9E8C9F36A22A030B
GO
Isoform 3 (identifier: Q6IQ23-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-447: MAAATVGRDT...WARAQKGDSR → MFPKACRTLAWLPDPFLPFLL
     915-915: V → VQ

Note: No experimental confirmation available.

Show »
Length:696
Mass (Da):79,104
Checksum:i2E0584D8332267A3
GO

Sequence cautioni

The sequence AAH33239.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
The sequence BAG62985.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti494 – 4941D → N in BAG62985. (PubMed:14702039)Curated
Sequence conflicti981 – 9811L → P in AAH71599. (PubMed:15489334)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti241 – 2411L → I.
Corresponds to variant rs35908144 [ dbSNP | Ensembl ].
VAR_032346
Natural varianti248 – 2481A → V.
Corresponds to variant rs16933529 [ dbSNP | Ensembl ].
VAR_032347
Natural varianti279 – 2791Q → R.
Corresponds to variant rs369819 [ dbSNP | Ensembl ].
VAR_032348
Natural varianti689 – 6891S → R.
Corresponds to variant rs61133161 [ dbSNP | Ensembl ].
VAR_061517
Natural varianti693 – 6931V → I.
Corresponds to variant rs34556458 [ dbSNP | Ensembl ].
VAR_032349

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 447447MAAAT…KGDSR → MFPKACRTLAWLPDPFLPFL L in isoform 3. 1 PublicationVSP_039543Add
BLAST
Alternative sequencei915 – 9151V → VQ in isoform 2 and isoform 3. 2 PublicationsVSP_025592

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK296371 mRNA. Translation: BAG59045.1.
AK301465 mRNA. Translation: BAG62985.1. Different initiation.
AC026639 Genomic DNA. No translation available.
AC116533 Genomic DNA. No translation available.
AC127033 Genomic DNA. No translation available.
BC033239 mRNA. Translation: AAH33239.1. Different initiation.
BC071599 mRNA. Translation: AAH71599.1.
CCDSiCCDS31434.1. [Q6IQ23-1]
RefSeqiNP_778228.3. NM_175058.4. [Q6IQ23-1]
UniGeneiHs.12332.
Hs.733020.

Genome annotation databases

EnsembliENST00000355661; ENSP00000347883; ENSG00000166689. [Q6IQ23-1]
GeneIDi144100.
KEGGihsa:144100.
UCSCiuc001mmn.3. human. [Q6IQ23-2]
uc001mmo.3. human. [Q6IQ23-1]
uc010rcv.2. human. [Q6IQ23-3]

Polymorphism databases

DMDMi215273867.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK296371 mRNA. Translation: BAG59045.1 .
AK301465 mRNA. Translation: BAG62985.1 . Different initiation.
AC026639 Genomic DNA. No translation available.
AC116533 Genomic DNA. No translation available.
AC127033 Genomic DNA. No translation available.
BC033239 mRNA. Translation: AAH33239.1 . Different initiation.
BC071599 mRNA. Translation: AAH71599.1 .
CCDSi CCDS31434.1. [Q6IQ23-1 ]
RefSeqi NP_778228.3. NM_175058.4. [Q6IQ23-1 ]
UniGenei Hs.12332.
Hs.733020.

3D structure databases

ProteinModelPortali Q6IQ23.
SMRi Q6IQ23. Positions 11-85, 157-282.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 126828. 7 interactions.
IntActi Q6IQ23. 3 interactions.
STRINGi 9606.ENSP00000347883.

PTM databases

PhosphoSitei Q6IQ23.

Polymorphism databases

DMDMi 215273867.

Proteomic databases

MaxQBi Q6IQ23.
PaxDbi Q6IQ23.
PRIDEi Q6IQ23.

Protocols and materials databases

DNASUi 144100.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000355661 ; ENSP00000347883 ; ENSG00000166689 . [Q6IQ23-1 ]
GeneIDi 144100.
KEGGi hsa:144100.
UCSCi uc001mmn.3. human. [Q6IQ23-2 ]
uc001mmo.3. human. [Q6IQ23-1 ]
uc010rcv.2. human. [Q6IQ23-3 ]

Organism-specific databases

CTDi 144100.
GeneCardsi GC11M016803.
HGNCi HGNC:27049. PLEKHA7.
HPAi HPA038610.
MIMi 612686. gene.
neXtProti NX_Q6IQ23.
PharmGKBi PA134894945.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG309520.
GeneTreei ENSGT00530000063012.
HOGENOMi HOG000077378.
HOVERGENi HBG103572.
InParanoidi Q6IQ23.
PhylomeDBi Q6IQ23.
TreeFami TF329090.

Miscellaneous databases

ChiTaRSi PLEKHA7. human.
GenomeRNAii 144100.
NextBioi 84824.
PROi Q6IQ23.
SOURCEi Search...

Gene expression databases

Bgeei Q6IQ23.
CleanExi HS_PLEKHA7.
ExpressionAtlasi Q6IQ23. baseline and differential.
Genevestigatori Q6IQ23.

Family and domain databases

Gene3Di 2.30.29.30. 1 hit.
InterProi IPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR001202. WW_dom.
[Graphical view ]
Pfami PF00169. PH. 1 hit.
[Graphical view ]
SMARTi SM00233. PH. 1 hit.
SM00456. WW. 2 hits.
[Graphical view ]
SUPFAMi SSF51045. SSF51045. 2 hits.
PROSITEi PS50003. PH_DOMAIN. 1 hit.
PS01159. WW_DOMAIN_1. 1 hit.
PS50020. WW_DOMAIN_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Tissue: Synovium and Thalamus.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Brain and Placenta.
  4. "Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry."
    Brill L.M., Salomon A.R., Ficarro S.B., Mukherji M., Stettler-Gill M., Peters E.C.
    Anal. Chem. 76:2763-2772(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.
  5. "Anchorage of microtubule minus ends to adherens junctions regulates epithelial cell-cell contacts."
    Meng W., Mushika Y., Ichii T., Takeichi M.
    Cell 135:948-959(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH KIAA1543 AND CTNND1.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-903 AND SER-907, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  7. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
    Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
    Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-604; SER-612; SER-903; SER-907 AND SER-986, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.
  9. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-903 AND SER-907, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPKHA7_HUMAN
AccessioniPrimary (citable) accession number: Q6IQ23
Secondary accession number(s): B4DK33, B4DWC3, Q86VZ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: November 25, 2008
Last modified: October 29, 2014
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3