Reviewed, UniProtKB/Swiss-Prot Q6IQ22 (RAB12_HUMAN)

Names and origin

Protein names

Recommended name:
    Putative Ras-related protein Rab-12

Gene names

Name:

RAB12

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	244 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Uncertain.

General annotation (Comments)

Subcellular location	Golgi apparatus membrane; Lipid-anchorBy similarity.
Sequence similarities	Belongs to the small GTPase superfamily. Rab family.
Caution	Could be the product of a pseudogene.
Sequence caution	The sequence AAH71600.2 differs from that shown. Reason: Miscellaneous discrepancy. Intron retention. The sequence AAH98407.1 differs from that shown. Reason: Miscellaneous discrepancy. Intron retention. The sequence BC050338 differs from that shown. Reason: Frameshift at position 195.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Golgi apparatus Membrane
Ligand	GTP-binding Nucleotide-binding
PTM	Lipoprotein Phosphoprotein Prenylation
Technical term	3D-structure
Gene Ontology (GO)
Biological process	protein transport Inferred from electronic annotation. Source: InterPro small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 244	244	Putative Ras-related protein Rab-12		PRO_0000271377
Regions
	Nucleotide binding	49 – 56	8	GTP By similarity
	Nucleotide binding	97 – 101	5	GTP By similarity
	Nucleotide binding	155 – 158	4	GTP By similarity
	Motif	71 – 79	9	Effector region By similarity
Amino acid modifications
	Modified residue	21	1	Phosphoserine Ref.4
	Modified residue	25	1	Phosphoserine Ref.4
	Modified residue	106	1	Phosphoserine By similarity
	Lipidation	243	1	S-geranylgeranyl cysteine By similarity
	Lipidation	244	1	S-geranylgeranyl cysteine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6IQ22-1 [UniParc]. Last modified January 9, 2007. Version 3. Checksum: B4DC65DD0DCB45C4	FASTA	244	27,248
	10 20 30 40 50 60 MDPGAALQRR AGGGGGLGAG SPALSGGQGR RRKQPPRPAD FKLQVIIIGS RGVGKTSLME 70 80 90 100 110 120 RFTDDTFCEA CKSTVGVDFK IKTVELRGKK IRLQIWDTAG QERFNSITSA YYRSAKGIIL 130 140 150 160 170 180 VYDITKKETF DDLPKWMKMI DKYASEDAEL LLVGNKLDCE TDREITRQQG EKFAQQITGM 190 200 210 220 230 240 RFCEASAKDN FNVDEIFLKL VDDILKKMPL DILRNELSNS ILSLQPEPEI PPELPPPRPH VRCC

References

[1]	"DNA sequence and analysis of human chromosome 18." Nusbaum C., Zody M.C., Borowsky M.L., Kamal M., Kodira C.D., Taylor T.D., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Abouelleil A., Allen N.R., Anderson S., Bloom T., Bugalter B., Butler J. , Cook A., DeCaprio D., Engels R., Garber M., Gnirke A., Hafez N., Hall J.L., Norman C.H., Itoh T., Jaffe D.B., Kuroki Y., Lehoczky J., Lui A., Macdonald P., Mauceli E., Mikkelsen T.S., Naylor J.W., Nicol R., Nguyen C., Noguchi H., O'Leary S.B., Piqani B., Smith C.L., Talamas J.A., Topham K., Totoki Y., Toyoda A., Wain H.M., Young S.K., Zeng Q., Zimmer A.R., Fujiyama A., Hattori M., Birren B.W., Sakaki Y., Lander E.S. Nature 437:551-555(2005) [PubMed: 16177791] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta.
[4]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-21 AND SER-25, MASS SPECTROMETRY.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AP001793 Genomic DNA. No translation available.
CH471113 Genomic DNA. Translation: EAX01616.1.
BC050338 mRNA. No translation available.
BC071600 mRNA. Translation: AAH71600.2. Sequence problems.
BC098407 mRNA. Translation: AAH98407.1. Sequence problems.

RefSeq

NP_001020471.2.

UniGene

Hs.270074

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2IL1	X-ray	2.10	A	36-208	[»]

SMR

Q6IQ22. Positions 38-208.

ModBase

Search...

PTM databases

PhosphoSite

Q6IQ22.

Proteomic databases

PRIDE

Q6IQ22.

Genome annotation databases

Ensembl

ENSG00000206418. Homo sapiens. [Contig view]

GeneID

201475.

KEGG

hsa:201475.

NMPDR

fig|9606.3.peg.14764.

Organism-specific databases

GeneCards

GC18P008600.

HGNC

HGNC:31332. RAB12.

PharmGKB

PA142671102.

GenAtlas

Search...

Phylogenomic databases

HOVERGEN

Q6IQ22.

Gene expression databases

ArrayExpress

Q6IQ22.

CleanEx

HS_RAB12.

Family and domain databases

InterPro

IPR003579. GTPase_Rab.
IPR013753. Ras.
IPR001806. Ras_trnsfrmng.
IPR005225. Small_GTP_bd.
[Graphical view]

Pfam

PF00071. Ras. 1 hit.
[Graphical view]

PRINTS

PR00449. RASTRNSFRMNG.

SMART

SM00175. RAB. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00231. small_GTP. 1 hit.

ProtoNet

Search...

Other Resources

NextBio

90147.

Entry information

Entry name

RAB12_HUMAN

Accession

Primary (citable) accession number: Q6IQ22
Secondary accession number(s): A6NEF5, Q4KMQ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	January 9, 2007
Last modified:	December 16, 2008
This is version 48 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)