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Q6IQ22

- RAB12_HUMAN

UniProt

Q6IQ22 - RAB12_HUMAN

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Protein

Ras-related protein Rab-12

Gene

RAB12

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab may play a role in protein transport from recycling endosomes to lysosomes regulating, for instance, the degradation of the transferrin receptor. Involved in autophagy (By similarity).By similarity

Enzyme regulationi

Rab activation is generally mediated by a guanine exchange factor (GEF), while inactivation through hydrolysis of bound GTP is catalyzed by a GTPase activating protein (GAP) (By similarity). That Rab is activated by DENND3, a guanine exchange factor.By similarity1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi49 – 579GTP
Nucleotide bindingi97 – 1015GTPBy similarity
Nucleotide bindingi155 – 1595GTP
Nucleotide bindingi187 – 1882GTP

GO - Molecular functioni

  1. GDP binding Source: UniProtKB
  2. GTP binding Source: UniProtKB-KW

GO - Biological processi

  1. autophagy Source: UniProtKB-KW
  2. cellular protein catabolic process Source: UniProtKB
  3. endosome to lysosome transport Source: UniProtKB
  4. protein transport Source: UniProtKB-KW
  5. small GTPase mediated signal transduction Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Autophagy, Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rab-12
Gene namesi
Name:RAB12
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 18

Organism-specific databases

HGNCiHGNC:31332. RAB12.

Subcellular locationi

Recycling endosome membrane By similarity; Lipid-anchor By similarity; Cytoplasmic side By similarity. Lysosome membrane By similarity; Lipid-anchor By similarity; Cytoplasmic side By similarity. Golgi apparatus membrane By similarity. Cytoplasmic vesicleautophagosome By similarity

GO - Cellular componenti

  1. Golgi apparatus Source: UniProtKB-KW
  2. lysosome Source: UniProtKB
  3. recycling endosome membrane Source: UniProtKB
  4. secretory granule Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Endosome, Golgi apparatus, Lysosome, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA142671102.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 244244Ras-related protein Rab-12PRO_0000271377Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine2 Publications
Modified residuei21 – 211PhosphoserineBy similarity
Modified residuei106 – 1061PhosphoserineBy similarity
Lipidationi243 – 2431S-geranylgeranyl cysteineBy similarity
Lipidationi244 – 2441S-geranylgeranyl cysteineBy similarity

Keywords - PTMi

Acetylation, Lipoprotein, Phosphoprotein, Prenylation

Proteomic databases

MaxQBiQ6IQ22.
PaxDbiQ6IQ22.
PRIDEiQ6IQ22.

PTM databases

PhosphoSiteiQ6IQ22.

Expressioni

Gene expression databases

BgeeiQ6IQ22.
CleanExiHS_RAB12.
GenevestigatoriQ6IQ22.

Organism-specific databases

HPAiHPA040727.

Interactioni

Subunit structurei

Interacts with RABIF and OPTN.2 Publications

Protein-protein interaction databases

BioGridi128387. 4 interactions.
STRINGi9606.ENSP00000331748.

Structurei

Secondary structure

1
244
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi40 – 489
Helixi55 – 628
Beta strandi77 – 8610
Beta strandi89 – 9810
Helixi102 – 1043
Helixi105 – 11410
Beta strandi116 – 1238
Helixi127 – 1315
Helixi133 – 14311
Beta strandi149 – 1557
Helixi157 – 1626
Helixi167 – 1759
Beta strandi181 – 1844
Turni187 – 1904
Helixi193 – 20614

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2IL1X-ray2.10A36-208[»]
ProteinModelPortaliQ6IQ22.
SMRiQ6IQ22. Positions 38-208.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ6IQ22.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi71 – 799Effector regionBy similarity

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiCOG1100.
GeneTreeiENSGT00740000115017.
HOGENOMiHOG000233968.
HOVERGENiHBG009351.
InParanoidiQ6IQ22.
KOiK07907.
OMAiPPRMRCC.
OrthoDBiEOG769ZKM.
PhylomeDBiQ6IQ22.
TreeFamiTF314097.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00449. RASTRNSFRMNG.
SMARTiSM00175. RAB. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6IQ22-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDPGAALQRR AGGGGGLGAG SPALSGGQGR RRKQPPRPAD FKLQVIIIGS
60 70 80 90 100
RGVGKTSLME RFTDDTFCEA CKSTVGVDFK IKTVELRGKK IRLQIWDTAG
110 120 130 140 150
QERFNSITSA YYRSAKGIIL VYDITKKETF DDLPKWMKMI DKYASEDAEL
160 170 180 190 200
LLVGNKLDCE TDREITRQQG EKFAQQITGM RFCEASAKDN FNVDEIFLKL
210 220 230 240
VDDILKKMPL DILRNELSNS ILSLQPEPEI PPELPPPRPH VRCC
Length:244
Mass (Da):27,248
Last modified:January 9, 2007 - v3
Checksum:iB4DC65DD0DCB45C4
GO

Sequence cautioni

The sequence AAH71600.2 differs from that shown. Reason: Intron retention.
The sequence AAH98407.1 differs from that shown. Reason: Intron retention.
The sequence BC050338 differs from that shown. Reason: Frameshift at position 195.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP001793 Genomic DNA. No translation available.
CH471113 Genomic DNA. Translation: EAX01616.1.
BC050338 mRNA. No translation available.
BC071600 mRNA. Translation: AAH71600.2. Sequence problems.
BC098407 mRNA. Translation: AAH98407.1. Sequence problems.
CCDSiCCDS42410.1.
RefSeqiNP_001020471.2. NM_001025300.2.
UniGeneiHs.270074.

Genome annotation databases

EnsembliENST00000329286; ENSP00000331748; ENSG00000206418.
GeneIDi201475.
KEGGihsa:201475.
UCSCiuc002knp.3. human.

Polymorphism databases

DMDMi122064944.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP001793 Genomic DNA. No translation available.
CH471113 Genomic DNA. Translation: EAX01616.1 .
BC050338 mRNA. No translation available.
BC071600 mRNA. Translation: AAH71600.2 . Sequence problems.
BC098407 mRNA. Translation: AAH98407.1 . Sequence problems.
CCDSi CCDS42410.1.
RefSeqi NP_001020471.2. NM_001025300.2.
UniGenei Hs.270074.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2IL1 X-ray 2.10 A 36-208 [» ]
ProteinModelPortali Q6IQ22.
SMRi Q6IQ22. Positions 38-208.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 128387. 4 interactions.
STRINGi 9606.ENSP00000331748.

PTM databases

PhosphoSitei Q6IQ22.

Polymorphism databases

DMDMi 122064944.

Proteomic databases

MaxQBi Q6IQ22.
PaxDbi Q6IQ22.
PRIDEi Q6IQ22.

Protocols and materials databases

DNASUi 201475.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000329286 ; ENSP00000331748 ; ENSG00000206418 .
GeneIDi 201475.
KEGGi hsa:201475.
UCSCi uc002knp.3. human.

Organism-specific databases

CTDi 201475.
GeneCardsi GC18P008600.
HGNCi HGNC:31332. RAB12.
HPAi HPA040727.
neXtProti NX_Q6IQ22.
PharmGKBi PA142671102.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG1100.
GeneTreei ENSGT00740000115017.
HOGENOMi HOG000233968.
HOVERGENi HBG009351.
InParanoidi Q6IQ22.
KOi K07907.
OMAi PPRMRCC.
OrthoDBi EOG769ZKM.
PhylomeDBi Q6IQ22.
TreeFami TF314097.

Miscellaneous databases

ChiTaRSi RAB12. human.
EvolutionaryTracei Q6IQ22.
GenomeRNAii 201475.
NextBioi 90147.
PROi Q6IQ22.

Gene expression databases

Bgeei Q6IQ22.
CleanExi HS_RAB12.
Genevestigatori Q6IQ22.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view ]
Pfami PF00071. Ras. 1 hit.
[Graphical view ]
PRINTSi PR00449. RASTRNSFRMNG.
SMARTi SM00175. RAB. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR00231. small_GTP. 1 hit.
PROSITEi PS51419. RAB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Placenta.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  5. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  6. "Family-wide characterization of the DENN domain Rab GDP-GTP exchange factors."
    Yoshimura S., Gerondopoulos A., Linford A., Rigden D.J., Barr F.A.
    J. Cell Biol. 191:367-381(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: ENZYME REGULATION.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Identification and characterisation of novel Mss4-binding Rab GTPases."
    Wixler V., Wixler L., Altenfeld A., Ludwig S., Goody R.S., Itzen A.
    Biol. Chem. 392:239-248(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RABIF.
  9. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
    Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
    Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Crystal structure of a predicted human GTPase in complex with GDP."
    Structural genomics consortium (SGC)
    Submitted (FEB-2009) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 36-208 IN COMPLEX WITH MAGNESIUM AND GDP.

Entry informationi

Entry nameiRAB12_HUMAN
AccessioniPrimary (citable) accession number: Q6IQ22
Secondary accession number(s): A6NEF5, Q4KMQ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: January 9, 2007
Last modified: October 29, 2014
This is version 104 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 18
    Human chromosome 18: entries, gene names and cross-references to MIM
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3