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Protein

N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D

Gene

NAPEPLD

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes N-acyl-phosphatidylethanolamines (NAPEs) to produce N-acylethanolamines (NAEs) and phosphatidic acid. Responsible for the generation of anandamide (N-arachidonoylethanolamine), the ligand of cannabinoid and vanilloid receptors (By similarity).By similarity

Catalytic activityi

An N-acylphosphatidylethanolamine + H2O = an N-acylethanolamine + a 1,2-diacylglycerol 3-phosphate.2 Publications

Cofactori

Zn2+By similarityNote: Binds 1 or 2 zinc ions per subunit.By similarity

Enzyme regulationi

Activity is stimulated by divalent cations.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi185Zinc 1Sequence analysis1
Metal bindingi187Zinc 1Sequence analysis1
Metal bindingi189Zinc 2Sequence analysis1
Metal bindingi190Zinc 2Sequence analysis1
Metal bindingi253Zinc 1Sequence analysis1
Metal bindingi343Zinc 2Sequence analysis1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processLipid degradation, Lipid metabolism, Phospholipid degradation, Phospholipid metabolism
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:ENSG00000161048-MONOMER.
BRENDAi3.1.4.54. 2681.
ReactomeiR-HSA-2466712. Biosynthesis of A2E, implicated in retinal degradation.

Chemistry databases

SwissLipidsiSLP:000001132.

Names & Taxonomyi

Protein namesi
Recommended name:
N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D (EC:3.1.4.54)
Short name:
N-acyl phosphatidylethanolamine phospholipase D
Short name:
NAPE-PLD
Short name:
NAPE-hydrolyzing phospholipase D
Gene namesi
Name:NAPEPLD
Synonyms:C7orf18
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

EuPathDBiHostDB:ENSG00000161048.11.
HGNCiHGNC:21683. NAPEPLD.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi152S → A: Almost no change in activity. 1 Publication1
Mutagenesisi207L → F: Loss of activity. 1 Publication1
Mutagenesisi380H → R: Loss of activity. 1 Publication1
Mutagenesisi389D → N: Almost no change in activity. 1 Publication1

Organism-specific databases

DisGeNETi222236.
OpenTargetsiENSG00000161048.
PharmGKBiPA162396960.

Polymorphism and mutation databases

BioMutaiNAPEPLD.
DMDMi167016292.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003181591 – 393N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase DAdd BLAST393

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ6IQ20.
MaxQBiQ6IQ20.
PaxDbiQ6IQ20.
PeptideAtlasiQ6IQ20.
PRIDEiQ6IQ20.

PTM databases

iPTMnetiQ6IQ20.
PhosphoSitePlusiQ6IQ20.

Expressioni

Gene expression databases

BgeeiENSG00000161048.
CleanExiHS_NAPEPLD.
ExpressionAtlasiQ6IQ20. baseline and differential.
GenevisibleiQ6IQ20. HS.

Organism-specific databases

HPAiHPA019832.
HPA024338.

Interactioni

Subunit structurei

Monomer.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-16143143,EBI-16143143

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi128793. 1 interactor.
DIPiDIP-61398N.
STRINGi9606.ENSP00000340093.

Structurei

Secondary structure

1393
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi65 – 68Combined sources4
Helixi97 – 103Combined sources7
Turni110 – 113Combined sources4
Beta strandi121 – 123Combined sources3
Beta strandi126 – 129Combined sources4
Beta strandi135 – 139Combined sources5
Beta strandi142 – 147Combined sources6
Beta strandi151 – 153Combined sources3
Beta strandi158 – 161Combined sources4
Beta strandi178 – 182Combined sources5
Turni188 – 190Combined sources3
Helixi193 – 202Combined sources10
Beta strandi207 – 212Combined sources6
Helixi216 – 221Combined sources6
Beta strandi226 – 230Combined sources5
Beta strandi235 – 238Combined sources4
Beta strandi241 – 249Combined sources9
Beta strandi257 – 260Combined sources4
Beta strandi269 – 273Combined sources5
Beta strandi278 – 281Combined sources4
Helixi291 – 297Combined sources7
Beta strandi301 – 307Combined sources7
Helixi314 – 317Combined sources4
Turni318 – 320Combined sources3
Helixi324 – 333Combined sources10
Beta strandi339 – 347Combined sources9
Beta strandi350 – 352Combined sources3
Helixi356 – 368Combined sources13
Turni372 – 374Combined sources3
Beta strandi383 – 385Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4QN9X-ray2.65A/B1-393[»]
ProteinModelPortaliQ6IQ20.
SMRiQ6IQ20.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NAPE-PLD family.Curated

Phylogenomic databases

eggNOGiKOG3798. Eukaryota.
COG2220. LUCA.
GeneTreeiENSGT00390000017990.
HOGENOMiHOG000267495.
HOVERGENiHBG054649.
InParanoidiQ6IQ20.
KOiK13985.
OMAiRWLIMEK.
OrthoDBiEOG091G0AHO.
PhylomeDBiQ6IQ20.
TreeFamiTF313520.

Family and domain databases

Gene3Di3.60.15.10. 1 hit.
InterProiView protein in InterPro
IPR001279. Metallo-B-lactamas.
IPR036866. Metallo-hydrolase/OxRdtase.
IPR024884. NAPE-PLD.
PfamiView protein in Pfam
PF12706. Lactamase_B_2. 1 hit.
PIRSFiPIRSF038896. NAPE-PLD. 1 hit.
SUPFAMiSSF56281. SSF56281. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6IQ20-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDENESNQSL MTSSQYPKEA VRKRQNSARN SGASDSSRFS RKSFKLDYRL
60 70 80 90 100
EEDVTKSKKG KDGRFVNPWP TWKNPSIPNV LRWLIMEKDH SSVPSSKEEL
110 120 130 140 150
DKELPVLKPY FITNPEEAGV REAGLRVTWL GHATVMVEMD ELIFLTDPIF
160 170 180 190 200
SSRASPSQYM GPKRFRRSPC TISELPPIDA VLISHNHYDH LDYNSVIALN
210 220 230 240 250
ERFGNELRWF VPLGLLDWMQ KCGCENVIEL DWWEENCVPG HDKVTFVFTP
260 270 280 290 300
SQHWCKRTLM DDNKVLWGSW SVLGPWNRFF FAGDTGYCPA FEEIGKRFGP
310 320 330 340 350
FDLAAIPIGA YEPRWFMKYQ HVDPEEAVRI HTDVQTKKSM AIHWGTFALA
360 370 380 390
NEHYLEPPVK LNEALERYGL NAEDFFVLKH GESRYLNNDD ENF
Length:393
Mass (Da):45,596
Last modified:February 5, 2008 - v2
Checksum:i5CE3114CC557B698
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti281Missing in CAI56779 (PubMed:17974005).Curated1
Sequence conflicti371N → Y in CAI56779 (PubMed:17974005).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_038695152S → A. Corresponds to variant dbSNP:rs12540583Ensembl.1
Natural variantiVAR_038694389D → N3 PublicationsCorresponds to variant dbSNP:rs3181009Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY357337 mRNA. Translation: AAR13673.1.
AB112352 mRNA. Translation: BAD02399.1.
CR936639 mRNA. Translation: CAI56779.1.
CH471070 Genomic DNA. Translation: EAW83314.1.
BC071604 mRNA. Translation: AAH71604.1.
CCDSiCCDS5729.1.
RefSeqiNP_001116310.1. NM_001122838.1.
NP_945341.3. NM_198990.4.
XP_005250271.2. XM_005250214.4.
XP_005250272.1. XM_005250215.2.
XP_005250275.1. XM_005250218.2.
XP_011514235.1. XM_011515933.2.
XP_016867343.1. XM_017011854.1.
XP_016867344.1. XM_017011855.1.
UniGeneiHs.324271.

Genome annotation databases

EnsembliENST00000341533; ENSP00000340093; ENSG00000161048.
ENST00000417955; ENSP00000407112; ENSG00000161048.
ENST00000422589; ENSP00000412376; ENSG00000161048.
ENST00000427257; ENSP00000392775; ENSG00000161048.
ENST00000465647; ENSP00000419188; ENSG00000161048.
ENST00000611100; ENSP00000482162; ENSG00000275723.
ENST00000622712; ENSP00000481285; ENSG00000275723.
ENST00000632159; ENSP00000488504; ENSG00000275723.
ENST00000632703; ENSP00000488648; ENSG00000275723.
ENST00000633783; ENSP00000487770; ENSG00000275723.
GeneIDi222236.
KEGGihsa:222236.
UCSCiuc003vbc.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiNAPEP_HUMAN
AccessioniPrimary (citable) accession number: Q6IQ20
Secondary accession number(s): Q5CZ87, Q769K1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: February 5, 2008
Last modified: November 22, 2017
This is version 118 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families