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Protein

Centriole, cilia and spindle-associated protein

Gene

CCSAP

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in microtubule (MT) stabilization and this stabilization involves the maintenance of NUMA1 at the spindle poles. Colocalizes with polyglutamylated MTs to promote MT stabilization and regulate bipolar spindle formation in mitosis. Binding of CCSAP to centrosomes and the spindle around centrosomes during mitosis inhibits MT depolymerization, thereby stabilizing the mitotic spindle (PubMed:26562023). May play a role in embryonic development. May be required for proper cilia beating (By similarity).By similarity1 Publication

GO - Molecular functioni

  • microtubule binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Names & Taxonomyi

Protein namesi
Recommended name:
Centriole, cilia and spindle-associated protein
Gene namesi
Name:CCSAP
Synonyms:C1orf96, CSAP
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:29578. CCSAP.

Subcellular locationi

GO - Cellular componenti

  • axon Source: UniProtKB
  • axoneme Source: UniProtKB
  • centriole Source: UniProtKB
  • centrosome Source: UniProtKB
  • ciliary basal body Source: UniProtKB
  • ciliary transition zone Source: UniProtKB
  • cilium Source: UniProtKB
  • mitotic spindle Source: UniProtKB
  • mitotic spindle astral microtubule Source: UniProtKB
  • spindle Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cilium, Cytoplasm, Cytoskeleton, Microtubule

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA142672480.

Polymorphism and mutation databases

BioMutaiCCSAP.
DMDMi145558871.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 270270Centriole, cilia and spindle-associated proteinPRO_0000284643Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineCombined sources

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ6IQ19.
MaxQBiQ6IQ19.
PaxDbiQ6IQ19.
PeptideAtlasiQ6IQ19.
PRIDEiQ6IQ19.

PTM databases

iPTMnetiQ6IQ19.
PhosphoSiteiQ6IQ19.

Expressioni

Gene expression databases

BgeeiENSG00000154429.
CleanExiHS_C1orf96.
GenevisibleiQ6IQ19. HS.

Organism-specific databases

HPAiHPA028402.
HPA043443.

Interactioni

Subunit structurei

Associates with microtubules; the association occurs on polyglutamylated tubulin (PubMed:22493317, PubMed:26562023).2 Publications

GO - Molecular functioni

  • microtubule binding Source: UniProtKB

Protein-protein interaction databases

STRINGi9606.ENSP00000284617.

Structurei

3D structure databases

ProteinModelPortaliQ6IQ19.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi9 – 157ST]-E-Y-X(3)-Y motif 1; required for efficient microtubule binding and stabilizationBy similarity
Motifi260 – 2667ST]-E-Y-X(3)-Y motif 2; required for efficient microtubule binding and stabilizationBy similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi78 – 847Poly-Pro

Sequence similaritiesi

Belongs to the CCSAP family.Curated

Phylogenomic databases

eggNOGiENOG410IH9C. Eukaryota.
ENOG410Y5JR. LUCA.
GeneTreeiENSGT00390000003512.
HOGENOMiHOG000013103.
HOVERGENiHBG100745.
InParanoidiQ6IQ19.
KOiK16454.
OMAiRYQEPRW.
OrthoDBiEOG091G14UJ.
PhylomeDBiQ6IQ19.
TreeFamiTF332378.

Family and domain databases

InterProiIPR029774. CSAP.
[Graphical view]
PANTHERiPTHR31022. PTHR31022. 1 hit.
PfamiPF15748. CCSAP. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6IQ19-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSPGSGVKSE YMKRYQEPRW EEYGPCYREL LHYRLGRRLL EQAHAPWLWD
60 70 80 90 100
DWGPAGSSED SASSESSGAG GPAPRCAPPS PPPPVEPATQ EEAERRARGA
110 120 130 140 150
PEEQDAEAGD AEAEDAEDAA LPALPVKDVE DKPEQQTRTR ETDKSPTSTE
160 170 180 190 200
PRQQPSALFA RGNRKAVKSP QRSSSKIKEN KHPFALYGWG EKQTDTGSQK
210 220 230 240 250
THNVCASAPV HEIHESALRA KNRRQVEKRK LVAQRQRAHS VDVEKNRKMK
260 270
ASSSENPWMT EYMRCYSARA
Length:270
Mass (Da):30,216
Last modified:April 17, 2007 - v2
Checksum:iDA40F8593A5354EC
GO
Isoform 2 (identifier: Q6IQ19-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-114: Missing.
     115-122: DAEDAALP → MVPVFTSS

Note: No experimental confirmation available.
Show »
Length:156
Mass (Da):17,791
Checksum:iF506FBFF5767B44B
GO
Isoform 3 (identifier: Q6IQ19-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     123-129: ALPVKDV → GNAWLRT
     130-270: Missing.

Note: No experimental confirmation available.
Show »
Length:129
Mass (Da):14,091
Checksum:i80F765DDB8E2A87B
GO
Isoform 4 (identifier: Q6IQ19-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     123-140: ALPVKDVEDKPEQQTRTR → GTRPGGEREGPARRDGLL
     141-270: Missing.

Note: No experimental confirmation available.
Show »
Length:140
Mass (Da):15,168
Checksum:iEBC9727A2C513903
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti28 – 281R → G in AAH71609 (PubMed:15489334).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti123 – 1231A → V.
Corresponds to variant rs6587326 [ dbSNP | Ensembl ].
VAR_059594

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 114114Missing in isoform 2. 1 PublicationVSP_024582Add
BLAST
Alternative sequencei115 – 1228DAEDAALP → MVPVFTSS in isoform 2. 1 PublicationVSP_024583
Alternative sequencei123 – 14018ALPVK…QTRTR → GTRPGGEREGPARRDGLL in isoform 4. 1 PublicationVSP_024584Add
BLAST
Alternative sequencei123 – 1297ALPVKDV → GNAWLRT in isoform 3. 1 PublicationVSP_024585
Alternative sequencei130 – 270141Missing in isoform 3. 1 PublicationVSP_024586Add
BLAST
Alternative sequencei141 – 270130Missing in isoform 4. 1 PublicationVSP_024587Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK123465 mRNA. Translation: BAC85619.1.
AK291437 mRNA. Translation: BAF84126.1.
AL117350 Genomic DNA. Translation: CAI22868.1.
AL117350 Genomic DNA. Translation: CAI22869.1.
CH471098 Genomic DNA. Translation: EAW69897.1.
BC015419 mRNA. Translation: AAH15419.1.
BC039241 mRNA. Translation: AAH39241.1.
BC060777 mRNA. Translation: AAH60777.1.
BC071609 mRNA. Translation: AAH71609.1.
CCDSiCCDS1577.1. [Q6IQ19-1]
RefSeqiNP_660300.3. NM_145257.4. [Q6IQ19-1]
UniGeneiHs.585011.

Genome annotation databases

EnsembliENST00000284617; ENSP00000284617; ENSG00000154429. [Q6IQ19-1]
ENST00000366686; ENSP00000355647; ENSG00000154429. [Q6IQ19-2]
ENST00000366687; ENSP00000355648; ENSG00000154429. [Q6IQ19-1]
GeneIDi126731.
KEGGihsa:126731.
UCSCiuc001htl.4. human. [Q6IQ19-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK123465 mRNA. Translation: BAC85619.1.
AK291437 mRNA. Translation: BAF84126.1.
AL117350 Genomic DNA. Translation: CAI22868.1.
AL117350 Genomic DNA. Translation: CAI22869.1.
CH471098 Genomic DNA. Translation: EAW69897.1.
BC015419 mRNA. Translation: AAH15419.1.
BC039241 mRNA. Translation: AAH39241.1.
BC060777 mRNA. Translation: AAH60777.1.
BC071609 mRNA. Translation: AAH71609.1.
CCDSiCCDS1577.1. [Q6IQ19-1]
RefSeqiNP_660300.3. NM_145257.4. [Q6IQ19-1]
UniGeneiHs.585011.

3D structure databases

ProteinModelPortaliQ6IQ19.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9606.ENSP00000284617.

PTM databases

iPTMnetiQ6IQ19.
PhosphoSiteiQ6IQ19.

Polymorphism and mutation databases

BioMutaiCCSAP.
DMDMi145558871.

Proteomic databases

EPDiQ6IQ19.
MaxQBiQ6IQ19.
PaxDbiQ6IQ19.
PeptideAtlasiQ6IQ19.
PRIDEiQ6IQ19.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000284617; ENSP00000284617; ENSG00000154429. [Q6IQ19-1]
ENST00000366686; ENSP00000355647; ENSG00000154429. [Q6IQ19-2]
ENST00000366687; ENSP00000355648; ENSG00000154429. [Q6IQ19-1]
GeneIDi126731.
KEGGihsa:126731.
UCSCiuc001htl.4. human. [Q6IQ19-1]

Organism-specific databases

CTDi126731.
GeneCardsiCCSAP.
H-InvDBHIX0001677.
HGNCiHGNC:29578. CCSAP.
HPAiHPA028402.
HPA043443.
neXtProtiNX_Q6IQ19.
PharmGKBiPA142672480.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IH9C. Eukaryota.
ENOG410Y5JR. LUCA.
GeneTreeiENSGT00390000003512.
HOGENOMiHOG000013103.
HOVERGENiHBG100745.
InParanoidiQ6IQ19.
KOiK16454.
OMAiRYQEPRW.
OrthoDBiEOG091G14UJ.
PhylomeDBiQ6IQ19.
TreeFamiTF332378.

Miscellaneous databases

GenomeRNAii126731.
PROiQ6IQ19.

Gene expression databases

BgeeiENSG00000154429.
CleanExiHS_C1orf96.
GenevisibleiQ6IQ19. HS.

Family and domain databases

InterProiIPR029774. CSAP.
[Graphical view]
PANTHERiPTHR31022. PTHR31022. 1 hit.
PfamiPF15748. CCSAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCCSAP_HUMAN
AccessioniPrimary (citable) accession number: Q6IQ19
Secondary accession number(s): A8K5X2
, Q6P9G2, Q6ZW85, Q8IXU1, Q96BM2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: April 17, 2007
Last modified: September 7, 2016
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.