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Protein

Speckle-type POZ protein-like

Gene

SPOPL

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins, but with relatively low efficiency. Cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes containing homodimeric SPOPL or the heterodimer formed by SPOP and SPOPL are less efficient than ubiquitin ligase complexes containing only SPOP. May function to down-regulate the activity of cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes that contain SPOP.1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • negative regulation of protein ubiquitination Source: UniProtKB
  • proteasome-mediated ubiquitin-dependent protein catabolic process Source: UniProtKB
  • protein ubiquitination involved in ubiquitin-dependent protein catabolic process Source: GO_Central
  • regulation of proteolysis Source: GO_Central

Keywordsi

Biological processUbl conjugation pathway

Enzyme and pathway databases

ReactomeiR-HSA-5632684. Hedgehog 'on' state.
SignaLinkiQ6IQ16.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Speckle-type POZ protein-like
Alternative name(s):
HIB homolog 2
Roadkill homolog 2
Gene namesi
Name:SPOPL
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:27934. SPOPL.

Subcellular locationi

GO - Cellular componenti

  • Cul3-RING ubiquitin ligase complex Source: UniProtKB
  • cytoplasm Source: GO_Central
  • nucleus Source: GO_Central
  • SCF ubiquitin ligase complex Source: GO_Central

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000144228.
PharmGKBiPA162404650.

Polymorphism and mutation databases

BioMutaiSPOPL.
DMDMi74736582.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002745881 – 392Speckle-type POZ protein-likeAdd BLAST392

Proteomic databases

EPDiQ6IQ16.
MaxQBiQ6IQ16.
PaxDbiQ6IQ16.
PeptideAtlasiQ6IQ16.
PRIDEiQ6IQ16.

PTM databases

iPTMnetiQ6IQ16.
PhosphoSitePlusiQ6IQ16.

Expressioni

Gene expression databases

BgeeiENSG00000144228.
CleanExiHS_SPOPL.
ExpressionAtlasiQ6IQ16. baseline and differential.
GenevisibleiQ6IQ16. HS.

Organism-specific databases

HPAiHPA034687.

Interactioni

Subunit structurei

Homodimer. Heterodimer with SPOP. Component of cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes containing homodimeric SPOPL or the heterodimer formed by SPOP and SPOPL. Interacts with CUL3 and H2AFY.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
KPNA5O151313EBI-2822161,EBI-540602

GO - Molecular functioni

Protein-protein interaction databases

BioGridi130923. 2 interactors.
IntActiQ6IQ16. 6 interactors.
STRINGi9606.ENSP00000280098.

Structurei

3D structure databases

ProteinModelPortaliQ6IQ16.
SMRiQ6IQ16.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini31 – 161MATHPROSITE-ProRule annotationAdd BLAST131
Domaini200 – 267BTBPROSITE-ProRule annotationAdd BLAST68

Sequence similaritiesi

Belongs to the Tdpoz family.Curated

Phylogenomic databases

eggNOGiKOG1987. Eukaryota.
ENOG410XQV8. LUCA.
GeneTreeiENSGT00390000000361.
HOGENOMiHOG000231621.
HOVERGENiHBG001393.
InParanoidiQ6IQ16.
KOiK10523.
OMAiQAADIME.
OrthoDBiEOG091G07ZM.
PhylomeDBiQ6IQ16.
TreeFamiTF313419.

Family and domain databases

CDDicd14734. SPOP_C. 1 hit.
Gene3Di2.60.210.10. 1 hit.
InterProiView protein in InterPro
IPR000210. BTB/POZ_dom.
IPR002083. MATH/TRAF_dom.
IPR011333. SKP1/BTB/POZ.
IPR034089. SPOP_C.
IPR008974. TRAF-like.
PfamiView protein in Pfam
PF00651. BTB. 1 hit.
PF00917. MATH. 1 hit.
SMARTiView protein in SMART
SM00225. BTB. 1 hit.
SM00061. MATH. 1 hit.
SUPFAMiSSF49599. SSF49599. 1 hit.
SSF54695. SSF54695. 1 hit.
PROSITEiView protein in PROSITE
PS50097. BTB. 1 hit.
PS50144. MATH. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6IQ16-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSREPTPPLP GDMSTGPIAE SWCYTQVKVV KFSYMWTINN FSFCREEMGE
60 70 80 90 100
VLKSSTFSSG PSDKMKWCLR VNPKGLDDES KDYLSLYLLL VSCPKSEVRA
110 120 130 140 150
KFKFSLLNAK REETKAMESQ RAYRFVQGKD WGFKKFIRRD FLLDEANGLL
160 170 180 190 200
PDDKLTLFCE VSVVQDSVNI SGHTNTNTLK VPECRLAEDL GNLWENTRFT
210 220 230 240 250
DCSFFVRGQE FKAHKSVLAA RSPVFNAMFE HEMEESKKNR VEINDLDPEV
260 270 280 290 300
FKEMMRFIYT GRAPNLDKMA DNLLAAADKY ALERLKVMCE EALCSNLSVE
310 320 330 340 350
NVADTLVLAD LHSAEQLKAQ AIDFINRCSV LRQLGCKDGK NWNSNQATDI
360 370 380 390
METSGWKSMI QSHPHLVAEA FRALASAQCP QFGIPRKRLK QS
Length:392
Mass (Da):44,647
Last modified:July 5, 2004 - v1
Checksum:iCEB4243BD732DFEF
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05371945R → Q. Corresponds to variant dbSNP:rs36099753Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC071613 mRNA. Translation: AAH71613.1.
CCDSiCCDS33298.1.
RefSeqiNP_001001664.1. NM_001001664.2.
UniGeneiHs.333297.

Genome annotation databases

EnsembliENST00000280098; ENSP00000280098; ENSG00000144228.
GeneIDi339745.
KEGGihsa:339745.
UCSCiuc002tvh.5. human.

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiSPOPL_HUMAN
AccessioniPrimary (citable) accession number: Q6IQ16
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 6, 2007
Last sequence update: July 5, 2004
Last modified: June 7, 2017
This is version 110 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  5. SIMILARITY comments
    Index of protein domains and families