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Protein

Speckle-type POZ protein-like

Gene

SPOPL

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins, but with relatively low efficiency. Cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes containing homodimeric SPOPL or the heterodimer formed by SPOP and SPOPL are less efficient than ubiquitin ligase complexes containing only SPOP. May function to down-regulate the activity of cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes that contain SPOP.1 Publication

Pathway:iprotein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Biological processi

  • negative regulation of protein ubiquitination Source: UniProtKB
  • proteasome-mediated ubiquitin-dependent protein catabolic process Source: UniProtKB
  • protein ubiquitination Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiREACT_268718. Hedgehog 'on' state.
SignaLinkiQ6IQ16.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Speckle-type POZ protein-like
Alternative name(s):
HIB homolog 2
Roadkill homolog 2
Gene namesi
Name:SPOPL
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:27934. SPOPL.

Subcellular locationi

GO - Cellular componenti

  • Cul3-RING ubiquitin ligase complex Source: UniProtKB
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA162404650.

Polymorphism and mutation databases

BioMutaiSPOPL.
DMDMi74736582.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 392392Speckle-type POZ protein-likePRO_0000274588Add
BLAST

Proteomic databases

MaxQBiQ6IQ16.
PaxDbiQ6IQ16.
PRIDEiQ6IQ16.

PTM databases

PhosphoSiteiQ6IQ16.

Expressioni

Gene expression databases

BgeeiQ6IQ16.
CleanExiHS_SPOPL.
ExpressionAtlasiQ6IQ16. baseline and differential.
GenevisibleiQ6IQ16. HS.

Organism-specific databases

HPAiHPA034687.

Interactioni

Subunit structurei

Homodimer. Heterodimer with SPOP. Component of cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes containing homodimeric SPOPL or the heterodimer formed by SPOP and SPOPL. Interacts with CUL3 and H2AFY.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
KPNA5O151313EBI-2822161,EBI-540602

Protein-protein interaction databases

BioGridi130923. 1 interaction.
IntActiQ6IQ16. 2 interactions.
STRINGi9606.ENSP00000280098.

Structurei

3D structure databases

ProteinModelPortaliQ6IQ16.
SMRiQ6IQ16. Positions 28-374.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini31 – 161131MATHPROSITE-ProRule annotationAdd
BLAST
Domaini200 – 26768BTBPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the Tdpoz family.Curated
Contains 1 BTB (POZ) domain.PROSITE-ProRule annotation
Contains 1 MATH domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG273048.
GeneTreeiENSGT00390000000361.
HOGENOMiHOG000231621.
HOVERGENiHBG001393.
InParanoidiQ6IQ16.
KOiK10523.
OMAiHEMEECT.
OrthoDBiEOG73NG3G.
PhylomeDBiQ6IQ16.
TreeFamiTF313419.

Family and domain databases

InterProiIPR000210. BTB/POZ-like.
IPR011333. BTB/POZ_fold.
IPR013069. BTB_POZ.
IPR002083. MATH.
IPR008974. TRAF-like.
[Graphical view]
PfamiPF00651. BTB. 1 hit.
PF00917. MATH. 1 hit.
[Graphical view]
SMARTiSM00225. BTB. 1 hit.
SM00061. MATH. 1 hit.
[Graphical view]
SUPFAMiSSF49599. SSF49599. 1 hit.
SSF54695. SSF54695. 1 hit.
PROSITEiPS50097. BTB. 1 hit.
PS50144. MATH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6IQ16-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSREPTPPLP GDMSTGPIAE SWCYTQVKVV KFSYMWTINN FSFCREEMGE
60 70 80 90 100
VLKSSTFSSG PSDKMKWCLR VNPKGLDDES KDYLSLYLLL VSCPKSEVRA
110 120 130 140 150
KFKFSLLNAK REETKAMESQ RAYRFVQGKD WGFKKFIRRD FLLDEANGLL
160 170 180 190 200
PDDKLTLFCE VSVVQDSVNI SGHTNTNTLK VPECRLAEDL GNLWENTRFT
210 220 230 240 250
DCSFFVRGQE FKAHKSVLAA RSPVFNAMFE HEMEESKKNR VEINDLDPEV
260 270 280 290 300
FKEMMRFIYT GRAPNLDKMA DNLLAAADKY ALERLKVMCE EALCSNLSVE
310 320 330 340 350
NVADTLVLAD LHSAEQLKAQ AIDFINRCSV LRQLGCKDGK NWNSNQATDI
360 370 380 390
METSGWKSMI QSHPHLVAEA FRALASAQCP QFGIPRKRLK QS
Length:392
Mass (Da):44,647
Last modified:July 5, 2004 - v1
Checksum:iCEB4243BD732DFEF
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti45 – 451R → Q.
Corresponds to variant rs36099753 [ dbSNP | Ensembl ].
VAR_053719

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC071613 mRNA. Translation: AAH71613.1.
CCDSiCCDS33298.1.
RefSeqiNP_001001664.1. NM_001001664.2.
UniGeneiHs.333297.

Genome annotation databases

EnsembliENST00000280098; ENSP00000280098; ENSG00000144228.
GeneIDi339745.
KEGGihsa:339745.
UCSCiuc002tvh.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC071613 mRNA. Translation: AAH71613.1.
CCDSiCCDS33298.1.
RefSeqiNP_001001664.1. NM_001001664.2.
UniGeneiHs.333297.

3D structure databases

ProteinModelPortaliQ6IQ16.
SMRiQ6IQ16. Positions 28-374.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi130923. 1 interaction.
IntActiQ6IQ16. 2 interactions.
STRINGi9606.ENSP00000280098.

PTM databases

PhosphoSiteiQ6IQ16.

Polymorphism and mutation databases

BioMutaiSPOPL.
DMDMi74736582.

Proteomic databases

MaxQBiQ6IQ16.
PaxDbiQ6IQ16.
PRIDEiQ6IQ16.

Protocols and materials databases

DNASUi339745.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000280098; ENSP00000280098; ENSG00000144228.
GeneIDi339745.
KEGGihsa:339745.
UCSCiuc002tvh.3. human.

Organism-specific databases

CTDi339745.
GeneCardsiGC02P139259.
HGNCiHGNC:27934. SPOPL.
HPAiHPA034687.
neXtProtiNX_Q6IQ16.
PharmGKBiPA162404650.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG273048.
GeneTreeiENSGT00390000000361.
HOGENOMiHOG000231621.
HOVERGENiHBG001393.
InParanoidiQ6IQ16.
KOiK10523.
OMAiHEMEECT.
OrthoDBiEOG73NG3G.
PhylomeDBiQ6IQ16.
TreeFamiTF313419.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiREACT_268718. Hedgehog 'on' state.
SignaLinkiQ6IQ16.

Miscellaneous databases

GenomeRNAii339745.
NextBioi97504.
PROiQ6IQ16.

Gene expression databases

BgeeiQ6IQ16.
CleanExiHS_SPOPL.
ExpressionAtlasiQ6IQ16. baseline and differential.
GenevisibleiQ6IQ16. HS.

Family and domain databases

InterProiIPR000210. BTB/POZ-like.
IPR011333. BTB/POZ_fold.
IPR013069. BTB_POZ.
IPR002083. MATH.
IPR008974. TRAF-like.
[Graphical view]
PfamiPF00651. BTB. 1 hit.
PF00917. MATH. 1 hit.
[Graphical view]
SMARTiSM00225. BTB. 1 hit.
SM00061. MATH. 1 hit.
[Graphical view]
SUPFAMiSSF49599. SSF49599. 1 hit.
SSF54695. SSF54695. 1 hit.
PROSITEiPS50097. BTB. 1 hit.
PS50144. MATH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  2. "Roadkill attenuates Hedgehog responses through degradation of Cubitus interruptus."
    Kent D., Bush E.W., Hooper J.E.
    Development 133:2001-2010(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.
  3. "A hedgehog-induced BTB protein modulates hedgehog signaling by degrading Ci/Gli transcription factor."
    Zhang Q., Zhang L., Wang B., Ou C.-Y., Chien C.-T., Jiang J.
    Dev. Cell 10:719-729(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.
  4. "Adaptor protein self-assembly drives the control of a cullin-RING ubiquitin ligase."
    Errington W.J., Khan M.Q., Bueler S.A., Rubinstein J.L., Chakrabartty A., Prive G.G.
    Structure 20:1141-1153(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, INTERACTION WITH CUL3 AND H2AFY.

Entry informationi

Entry nameiSPOPL_HUMAN
AccessioniPrimary (citable) accession number: Q6IQ16
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 6, 2007
Last sequence update: July 5, 2004
Last modified: July 22, 2015
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.