Q6IQ16 (SPOPL_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Speckle-type POZ protein-like

Alternative name(s):
HIB homolog 2
Roadkill homolog 2

Gene names

Name:

SPOPL

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	392 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins, but with relatively low efficiency. Cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes containing homodimeric SPOPL or the heterodimer formed by SPOP and SPOPL are less efficient than ubiquitin ligase complexes containing only SPOP. May function to down-regulate the activity of cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes that contain SPOP. Ref.4
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Homodimer. Heterodimer with SPOP. Component of cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes containing homodimeric SPOPL or the heterodimer formed by SPOP and SPOPL. Interacts with CUL3 and H2AFY. Ref.4
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the Tdpoz family. Contains 1 BTB (POZ) domain. Contains 1 MATH domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of protein ubiquitination Inferred from mutant phenotype Ref.4. Source: UniProtKB proteasomal ubiquitin-dependent protein catabolic process Inferred from mutant phenotype Ref.4. Source: UniProtKB protein ubiquitination Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	Cul3-RING ubiquitin ligase complex Inferred from direct assay Ref.4. Source: UniProtKB nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 392	392	Speckle-type POZ protein-like		PRO_0000274588
Regions
	Domain	31 – 161	131	MATH
	Domain	200 – 267	68	BTB
Natural variations
	Natural variant	45	1	R → Q. Corresponds to variant rs36099753 [ dbSNP | Ensembl ].		VAR_053719

Sequences

	Sequence		Length	Mass (Da)
	Q6IQ16 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: CEB4243BD732DFEF	FASTA	392	44,647
	10 20 30 40 50 60 MSREPTPPLP GDMSTGPIAE SWCYTQVKVV KFSYMWTINN FSFCREEMGE VLKSSTFSSG 70 80 90 100 110 120 PSDKMKWCLR VNPKGLDDES KDYLSLYLLL VSCPKSEVRA KFKFSLLNAK REETKAMESQ 130 140 150 160 170 180 RAYRFVQGKD WGFKKFIRRD FLLDEANGLL PDDKLTLFCE VSVVQDSVNI SGHTNTNTLK 190 200 210 220 230 240 VPECRLAEDL GNLWENTRFT DCSFFVRGQE FKAHKSVLAA RSPVFNAMFE HEMEESKKNR 250 260 270 280 290 300 VEINDLDPEV FKEMMRFIYT GRAPNLDKMA DNLLAAADKY ALERLKVMCE EALCSNLSVE 310 320 330 340 350 360 NVADTLVLAD LHSAEQLKAQ AIDFINRCSV LRQLGCKDGK NWNSNQATDI METSGWKSMI 370 380 390 QSHPHLVAEA FRALASAQCP QFGIPRKRLK QS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[2]	"Roadkill attenuates Hedgehog responses through degradation of Cubitus interruptus." Kent D., Bush E.W., Hooper J.E. Development 133:2001-2010(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION.
[3]	"A hedgehog-induced BTB protein modulates hedgehog signaling by degrading Ci/Gli transcription factor." Zhang Q., Zhang L., Wang B., Ou C.-Y., Chien C.-T., Jiang J. Dev. Cell 10:719-729(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION.
[4]	"Adaptor protein self-assembly drives the control of a cullin-RING ubiquitin ligase." Errington W.J., Khan M.Q., Bueler S.A., Rubinstein J.L., Chakrabartty A., Prive G.G. Structure 20:1141-1153(2012) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBUNIT, INTERACTION WITH CUL3 AND H2AFY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC071613 mRNA. Translation: AAH71613.1.
IPI	IPI00419928.
RefSeq	NP_001001664.1. NM_001001664.2.
UniGene	Hs.333297.
3D structure databases
HSSP	HSSP built from PDB template 2CR2 based on UniProtKB O43791.
ProteinModelPortal	Q6IQ16.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q6IQ16. 1 interaction.
STRING	9606.ENSP00000280098.
PTM databases
PhosphoSite	Q6IQ16.
Polymorphism databases
DMDM	74736582.
Proteomic databases
PaxDb	Q6IQ16.
PRIDE	Q6IQ16.
Protocols and materials databases
DNASU	339745.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000280098; ENSP00000280098; ENSG00000144228.
GeneID	339745.
KEGG	hsa:339745.
UCSC	uc002tvh.3. human.
Organism-specific databases
CTD	339745.
GeneCards	GC02P139259.
HGNC	HGNC:27934. SPOPL.
HPA	HPA034687.
neXtProt	NX_Q6IQ16.
PharmGKB	PA162404650.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG273048.
HOGENOM	HOG000231621.
HOVERGEN	HBG001393.
InParanoid	Q6IQ16.
KO	K10523.
OMA	MEESKKX.
OrthoDB	EOG42BX8K.
PhylomeDB	Q6IQ16.
Enzyme and pathway databases
UniPathway	UPA00143.
Gene expression databases
ArrayExpress	Q6IQ16.
Bgee	Q6IQ16.
CleanEx	HS_SPOPL.
Genevestigator	Q6IQ16.
Family and domain databases
Gene3D	3.30.710.10. 1 hit.
InterPro	IPR000210. BTB/POZ-like. IPR011333. BTB/POZ_fold. IPR013069. BTB_POZ. IPR002083. MATH. IPR008974. TRAF-like. [Graphical view]
Pfam	PF00651. BTB. 1 hit. PF00917. MATH. 1 hit. [Graphical view]
SMART	SM00225. BTB. 1 hit. SM00061. MATH. 1 hit. [Graphical view]
SUPFAM	SSF54695. BTB/POZ_fold. 1 hit. SSF49599. Traf_like. 1 hit.
PROSITE	PS50097. BTB. 1 hit. PS50144. MATH. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	339745.
NextBio	97504.

Entry information

Entry name

SPOPL_HUMAN

Accession

Primary (citable) accession number: Q6IQ16

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 6, 2007
Last sequence update:	July 5, 2004
Last modified:	May 1, 2013
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.