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Q6IN85 (P4R3A_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine/threonine-protein phosphatase 4 regulatory subunit 3A
Alternative name(s):
SMEK homolog 1
Gene names
Name:SMEK1
Synonyms:KIAA2010, PP4R3A, PPP4R3A
ORF Names:MSTP033
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length833 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Regulatory subunit of serine/threonine-protein phosphatase 4. May regulate the activity of PPP4C at centrosomal microtubule organizing centers. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on 'Ser-140' (gamma-H2AFX) generated during DNA replication and required for DNA DSB repair. Ref.10

Subunit structure

Serine/threonine-protein phosphatase 4 (PP4) occurs in different assemblies of the catalytic and one or more regulatory subunits. Component of the PP4 complex PPP4C-PPP4R2-PPP4R3A. Interacts with PPP4C; the interaction requires PPP4R2. Ref.8 Ref.10

Subcellular location

Cytoplasm. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome. Nucleus. Note: In interphase localized in the cytoplasm and in the nucleus (with higher levels). During metaphase located in pericentriolar regions. Ref.9

Sequence similarities

Belongs to the SMEK family.

Contains 1 WH1 domain.

Sequence caution

The sequence AAG39284.1 differs from that shown. Reason: Erroneous translation. Wrong choice of frame.

The sequence BAA91960.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence BAA91960.1 differs from that shown. Reason: Probable intron retention.

The sequence BAB14877.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence BAC23106.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q6IN85-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: No experimental confirmation available.
Isoform 2 (identifier: Q6IN85-2)

The sequence of this isoform differs from the canonical sequence as follows:
     410-422: Missing.
Note: No experimental confirmation available.
Isoform 4 (identifier: Q6IN85-4)

The sequence of this isoform differs from the canonical sequence as follows:
     67-305: Missing.
Note: No experimental confirmation available.
Isoform 5 (identifier: Q6IN85-5)

The sequence of this isoform differs from the canonical sequence as follows:
     410-422: Missing.
     554-833: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 833833Serine/threonine-protein phosphatase 4 regulatory subunit 3A
PRO_0000254598

Regions

Domain1 – 100100WH1

Amino acid modifications

Modified residue1171Phosphoserine Ref.11
Modified residue6551N6-acetyllysine Ref.14
Modified residue7411Phosphoserine Ref.16
Modified residue7711Phosphoserine Ref.11 Ref.13
Modified residue7741Phosphoserine Ref.13
Modified residue7771Phosphoserine Ref.13

Natural variations

Alternative sequence67 – 305239Missing in isoform 4.
VSP_021252
Alternative sequence410 – 42213Missing in isoform 2 and isoform 5.
VSP_021253
Alternative sequence554 – 833280Missing in isoform 5.
VSP_021256

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: EDDB4E1F47197A94

FASTA83395,368
        10         20         30         40         50         60 
MTDTRRRVKV YTLNEDRQWD DRGTGHVSSG YVERLKGMSL LVRAESDGSL LLESKINPNT 

        70         80         90        100        110        120 
AYQKQQDTLI VWSEAENYDL ALSFQEKAGC DEIWEKICQV QGKDPSVDIT QDLVDESEEE 

       130        140        150        160        170        180 
RFDDMSSPGL ELPSCELSRL EEIAELVASS LPSPLRREKL ALALENEGYI KKLLELFHVC 

       190        200        210        220        230        240 
EDLENIEGLH HLYEIIKGIF LLNRTALFEV MFSEECIMDV IGCLEYDPAL SQPRKHREFL 

       250        260        270        280        290        300 
TKTAKFKEVI PISDPELKQK IHQTYRVQYI QDMVLPTPSV FEENMLSTLH SFIFFNKVEI 

       310        320        330        340        350        360 
VGMLQEDEKF LTDLFAQLTD EATDEEKRQE LVNFLKEFCA FSQTLQPQNR DAFFKTLSNM 

       370        380        390        400        410        420 
GILPALEVIL GMDDTQVRSA ATDIFSYLVE YNPSMVREFV MQEAQQNDDV SKKLTEQKIT 

       430        440        450        460        470        480 
SKDILLINLI IEHMICDTDP ELGGAVQLMG LLRTLVDPEN MLATANKTEK TEFLGFFYKH 

       490        500        510        520        530        540 
CMHVLTAPLL ANTTEDKPSK DDFQTAQLLA LVLELLTFCV EHHTYHIKNY IINKDILRRV 

       550        560        570        580        590        600 
LVLMASKHAF LALCALRFKR KIIGLKDEFY NRYIMKSFLF EPVVKAFLNN GSRYNLMNSA 

       610        620        630        640        650        660 
IIEMFEFIRV EDIKSLTAHV IENYWKALED VDYVQTFKGL KLRFEQQRER QDNPKLDSMR 

       670        680        690        700        710        720 
SILRNHRYRR DARTLEDEEE MWFNTDEDDM EDGEAVVSPS DKTKNDDDIM DPISKFMERK 

       730        740        750        760        770        780 
KLKESEEKEV LLKTNLSGRQ SPSFKLSLSS GTKTNLTSQS STTNLPGSPG SPGSPGSPGS 

       790        800        810        820        830 
PGSVPKNTSQ TAAITTKGGL VGLVDYPDDD EDDDEDEDKE DTLPLSKKAK FDS 

« Hide

Isoform 2 [UniParc].

Checksum: B87BA2CA6C7121C4
Show »

FASTA82093,897
Isoform 4 [UniParc].

Checksum: 571E51214496F24F
Show »

FASTA59467,812
Isoform 5 [UniParc].

Checksum: 0C55DF4DB36B8908
Show »

FASTA54062,020

References

« Hide 'large scale' references
[1]"The nucleotide sequence of a long cDNA clone isolated from human."
Nagase T., Kikuno R., Ohara O.
Submitted (NOV-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Brain.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 5), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 98-833 (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 165-422 (ISOFORM 1).
Tissue: Ileal mucosa and Placenta.
[3]"The DNA sequence and analysis of human chromosome 14."
Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H. expand/collapse author list , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J.
Nature 421:601-607(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 4).
Tissue: Eye.
[5]"Full-length cDNA libraries and normalization."
Li W.B., Gruber C., Jessee J., Polayes D.
Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 238-833.
Tissue: Neuroblastoma.
[6]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 667-833.
Tissue: Melanoma.
[7]Liu B., Liu Y.Q., Wang X.Y., Zhao B., Sheng H., Zhao X.W., Liu S., Xu Y.Y., Ye J., Song L., Gao Y., Zhang C.L., Zhang J., Wei Y.J., Cao H.Q., Zhao Y., Liu L.S., Ding J.F. expand/collapse author list , Gao R.L., Wu Q.Y., Qiang B.Q., Yuan J.G., Liew C.C., Zhao M.S., Hui R.T.
Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 808-833.
Tissue: Aorta.
[8]"A novel, evolutionarily conserved protein phosphatase complex involved in cisplatin sensitivity."
Gingras A.-C., Caballero M., Zarske M., Sanchez A., Hazbun T.R., Fields S., Sonenberg N., Hafen E., Raught B., Aebersold R.
Mol. Cell. Proteomics 4:1725-1740(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE PPP4C-PPP4R2-PPP4R3A COMPLEX, INTERACTION WITH PPP4C, IDENTIFICATION BY MASS SPECTROMETRY.
[9]"Depletion of protein phosphatase 4 in human cells reveals essential roles in centrosome maturation, cell migration and the regulation of Rho GTPases."
Martin-Granados C., Philp A., Oxenham S.K., Prescott A.R., Cohen P.T.W.
Int. J. Biochem. Cell Biol. 40:2315-2332(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[10]"A PP4-phosphatase complex dephosphorylates gamma-H2AX generated during DNA replication."
Chowdhury D., Xu X., Zhong X., Ahmed F., Zhong J., Liao J., Dykxhoorn D.M., Weinstock D.M., Pfeifer G.P., Lieberman J.
Mol. Cell 31:33-46(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE PPP4C-PPP4R2-PPP4R3A COMPLEX, FUNCTION OF THE PPP4C-PPP4R2-PPP4R3A COMPLEX.
[11]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-117 AND SER-771, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[12]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[13]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-771; SER-774 AND SER-777, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
[14]"Lysine acetylation targets protein complexes and co-regulates major cellular functions."
Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-655, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[15]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[16]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-741, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB095930 mRNA. Translation: BAC23106.1. Different initiation.
AK000714 mRNA. Translation: BAA91338.1.
AK001885 mRNA. Translation: BAA91960.1. Sequence problems.
AK024297 mRNA. Translation: BAB14877.1. Different initiation.
AL133153 Genomic DNA. No translation available.
BC038932 mRNA. Translation: AAH38932.1.
BC072409 mRNA. Translation: AAH72409.1.
BX248247 mRNA. Translation: CAD62575.1.
AL832921 mRNA. Translation: CAH10634.1.
AF113213 mRNA. Translation: AAG39284.1. Sequence problems.
CCDSCCDS61532.1. [Q6IN85-4]
CCDS9895.1. [Q6IN85-2]
RefSeqNP_001271209.1. NM_001284280.1. [Q6IN85-2]
NP_001271210.1. NM_001284281.1. [Q6IN85-4]
XP_005267899.1. XM_005267842.1. [Q6IN85-1]
XP_006720257.1. XM_006720194.1. [Q6IN85-1]
UniGeneHs.533887.

3D structure databases

ProteinModelPortalQ6IN85.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid120803. 20 interactions.
IntActQ6IN85. 5 interactions.
MINTMINT-4827313.

PTM databases

PhosphoSiteQ6IN85.

Polymorphism databases

DMDM74736507.

Proteomic databases

MaxQBQ6IN85.
PaxDbQ6IN85.
PRIDEQ6IN85.

Protocols and materials databases

DNASU55671.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000337238; ENSP00000337125; ENSG00000100796. [Q6IN85-2]
ENST00000428424; ENSP00000392704; ENSG00000100796. [Q6IN85-4]
ENST00000554684; ENSP00000450864; ENSG00000100796. [Q6IN85-2]
ENST00000554943; ENSP00000450883; ENSG00000100796. [Q6IN85-1]
ENST00000555462; ENSP00000450891; ENSG00000100796. [Q6IN85-4]
GeneID55671.
KEGGhsa:55671.
UCSCuc001xzm.3. human. [Q6IN85-2]
uc001xzn.3. human. [Q6IN85-1]
uc001xzq.1. human. [Q6IN85-5]
uc010atz.3. human. [Q6IN85-4]

Organism-specific databases

CTD55671.
GeneCardsGC14M091923.
H-InvDBHIX0202046.
HGNCHGNC:20219. SMEK1.
HPAHPA002568.
MIM610351. gene.
neXtProtNX_Q6IN85.
PharmGKBPA162403940.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG314899.
HOVERGENHBG056624.
InParanoidQ6IN85.
KOK17491.
OMAIEMFEYV.
OrthoDBEOG7ZSHS7.
PhylomeDBQ6IN85.
TreeFamTF315190.

Enzyme and pathway databases

SignaLinkQ6IN85.

Gene expression databases

ArrayExpressQ6IN85.
BgeeQ6IN85.
GenevestigatorQ6IN85.

Family and domain databases

Gene3D2.30.29.30. 1 hit.
InterProIPR016024. ARM-type_fold.
IPR006887. DUF625.
IPR011993. PH_like_dom.
[Graphical view]
PfamPF04802. SMK-1. 1 hit.
[Graphical view]
SUPFAMSSF48371. SSF48371. 1 hit.
ProtoNetSearch...

Other

ChiTaRSSMEK1. human.
GenomeRNAi55671.
NextBio60444.
PROQ6IN85.
SOURCESearch...

Entry information

Entry nameP4R3A_HUMAN
AccessionPrimary (citable) accession number: Q6IN85
Secondary accession number(s): Q69YK6 expand/collapse secondary AC list , Q86U23, Q86YI7, Q8IVG1, Q9H3F1, Q9H7U8, Q9NV01, Q9NWP1
Entry history
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: July 5, 2004
Last modified: July 9, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 14

Human chromosome 14: entries, gene names and cross-references to MIM