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Protein

WAS/WASL-interacting protein family member 1

Gene

Wipf1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in the reorganization of the actin cytoskeleton. Contributes with NCK1 and GRB2 in the recruitment and activation of WASL. Plays a role in the formation of cell ruffles (By similarity). May participate in regulating the subcellular localization of WASL, resulting in the disassembly of stress fibers in favor of filopodia formation.By similarity1 Publication

GO - Biological processi

  • actin cytoskeleton organization Source: RGD
  • actin polymerization or depolymerization Source: InterPro
  • positive regulation of protein export from nucleus Source: RGD
Complete GO annotation...

Keywords - Ligandi

Actin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
WAS/WASL-interacting protein family member 1
Alternative name(s):
Wiskott-Aldrich syndrome protein-interacting protein
Short name:
WASP-interacting protein
Gene namesi
Name:Wipf1
Synonyms:Waspip, Wip
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620887. Wipf1.

Subcellular locationi

  • Cytoplasmic vesicle 1 Publication
  • Cytoplasmcytoskeleton 1 Publication
  • Cell projectionruffle By similarity

  • Note: Vesicle surfaces and along actin tails. Colocalizes with actin stress fibers. When coexpressed with WASL, no longer associated with actin filaments but accumulated in perinuclear and cortical areas like WASL.

GO - Cellular componenti

  • actin filament Source: RGD
  • cytoplasmic, membrane-bounded vesicle Source: UniProtKB-SubCell
  • ruffle Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cytoplasm, Cytoplasmic vesicle, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 487487WAS/WASL-interacting protein family member 1PRO_0000065943Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei138 – 1381PhosphoserineBy similarity
Modified residuei324 – 3241PhosphoserineCombined sources
Modified residuei329 – 3291PhosphothreonineBy similarity
Modified residuei334 – 3341PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ6IN36.
PRIDEiQ6IN36.

PTM databases

iPTMnetiQ6IN36.
PhosphoSiteiQ6IN36.

Expressioni

Tissue specificityi

Isoforms were differentially expressed. One isoform was ubiquitously expressed, another was muscle-specific and another was expressed in the liver, heart and testis.

Interactioni

Subunit structurei

Binds to WAS within the N-terminal region, at a site distinct from the CDC42-binding site. Binds profilin and actin. Interacts with DBNL (By similarity). Binds to WASL.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Dlg4P310165EBI-6986245,EBI-375655
Dnm1P215752EBI-6986245,EBI-80070
WaslO088162EBI-6986245,EBI-6142604

Protein-protein interaction databases

IntActiQ6IN36. 9 interactions.
MINTiMINT-3387626.
STRINGi10116.ENSRNOP00000024922.

Structurei

3D structure databases

ProteinModelPortaliQ6IN36.
SMRiQ6IN36. Positions 29-60, 435-479.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini32 – 4918WH2PROSITE-ProRule annotationAdd
BLAST
Repeati336 – 34510XRSGPXPPXP motif 1
Repeati358 – 36710XRSGPXPPXP motif 2
Repeati394 – 40310XRSGPXPPXP motif 3

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni45 – 484Binds actinBy similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi2 – 1312Poly-ProAdd
BLAST
Compositional biasi64 – 10340Gly-richAdd
BLAST
Compositional biasi122 – 486365Pro-richAdd
BLAST
Compositional biasi228 – 2325Poly-Ser

Sequence similaritiesi

Belongs to the verprolin family.Curated
Contains 1 WH2 domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG4462. Eukaryota.
ENOG4111FVT. LUCA.
HOGENOMiHOG000231914.
HOVERGENiHBG054157.
InParanoidiQ6IN36.
KOiK19475.
OrthoDBiEOG7PS1GQ.
PhylomeDBiQ6IN36.
TreeFamiTF332135.

Family and domain databases

InterProiIPR003124. WH2_dom.
IPR028295. WIP.
[Graphical view]
PANTHERiPTHR23202:SF32. PTHR23202:SF32. 2 hits.
PfamiPF02205. WH2. 1 hit.
[Graphical view]
SMARTiSM00246. WH2. 1 hit.
[Graphical view]
PROSITEiPS51082. WH2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced.

Isoform 1 (identifier: Q6IN36-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPVPPPPAPP PPPTFALANT EKPSLNKTEQ AGRNALLSDI SKGKKLKKTV
60 70 80 90 100
TNDRSAPILD KPKGAGGGYG GGSGGGGGGG SSGGGGNFGG GGPPGLGGLF
110 120 130 140 150
QAGMPKLRST ANRDNDSGGS RPPILPPGGR ATSAKPFSSP SGPGRFPAPS
160 170 180 190 200
PGHRSGPPEP PRNRMPPPRP DVGSKPDSLP PPVPNTPRPI PSSLHNRGSP
210 220 230 240 250
AGLGAPRPPF PGNRGAAFGA GSVRQNLSSS SSPFPRPPLP PTPSRALDDK
260 270 280 290 300
PPPPPPPVGN RPSMHREAVP PPPSQNSKPP VPSTPRPGAG SQAPPPPPPS
310 320 330 340 350
RPGPPPLPPT SSDEIPRLPQ RNLSLTSPTP PLPSPGRSGP LPPPPTERPP
360 370 380 390 400
PPVRDPPGRS GPLPPPPPIN RNGSTARALP ATPQLPSRSG MDSPRSGPRP
410 420 430 440 450
PLPPDRPGAG APPPPPPSTS VRNGFQDSSC EDEWESRFYF HPISDLPPPE
460 470 480
PYVPTTKTYP SKVARSESRS GSNRRERGAP PLPPIPR
Length:487
Mass (Da):49,751
Last modified:March 1, 2005 - v2
Checksum:iBF505071BB7012FE
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti139 – 1391S → P in AAH72475 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ303456 mRNA. Translation: CAC22282.1.
BC072475 mRNA. Translation: AAH72475.1.
RefSeqiNP_476540.2. NM_057192.2.
UniGeneiRn.161803.

Genome annotation databases

GeneIDi117538.
KEGGirno:117538.
UCSCiRGD:620887. rat. [Q6IN36-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ303456 mRNA. Translation: CAC22282.1.
BC072475 mRNA. Translation: AAH72475.1.
RefSeqiNP_476540.2. NM_057192.2.
UniGeneiRn.161803.

3D structure databases

ProteinModelPortaliQ6IN36.
SMRiQ6IN36. Positions 29-60, 435-479.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ6IN36. 9 interactions.
MINTiMINT-3387626.
STRINGi10116.ENSRNOP00000024922.

PTM databases

iPTMnetiQ6IN36.
PhosphoSiteiQ6IN36.

Proteomic databases

PaxDbiQ6IN36.
PRIDEiQ6IN36.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi117538.
KEGGirno:117538.
UCSCiRGD:620887. rat. [Q6IN36-1]

Organism-specific databases

CTDi7456.
RGDi620887. Wipf1.

Phylogenomic databases

eggNOGiKOG4462. Eukaryota.
ENOG4111FVT. LUCA.
HOGENOMiHOG000231914.
HOVERGENiHBG054157.
InParanoidiQ6IN36.
KOiK19475.
OrthoDBiEOG7PS1GQ.
PhylomeDBiQ6IN36.
TreeFamiTF332135.

Miscellaneous databases

PROiQ6IN36.

Family and domain databases

InterProiIPR003124. WH2_dom.
IPR028295. WIP.
[Graphical view]
PANTHERiPTHR23202:SF32. PTHR23202:SF32. 2 hits.
PfamiPF02205. WH2. 1 hit.
[Graphical view]
SMARTiSM00246. WH2. 1 hit.
[Graphical view]
PROSITEiPS51082. WH2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The rat homologue of Wiskott-Aldrich syndrome protein (WASP)-interacting protein (WIP) associates with actin filaments, recruits N-WASP from the nucleus, and mediates mobilization of actin from stress fibers in favor of filopodia formation."
    Vetterkind S., Miki H., Takenawa T., Klawitz I., Scheidtmann K.-H., Preuss U.
    J. Biol. Chem. 277:87-95(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH WASL.
    Strain: Fischer 344.
    Tissue: Brain.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung.
  3. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-324, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiWIPF1_RAT
AccessioniPrimary (citable) accession number: Q6IN36
Secondary accession number(s): Q8VDA4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: March 1, 2005
Last modified: June 8, 2016
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.