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Protein

Protein phosphatase 1 regulatory subunit 15A

Gene

Ppp1r15a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Recruits the serine/threonine-protein phosphatase PP1 to dephosphorylate the translation initiation factor eIF-2A/EIF2S1, thereby reversing the shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress. Down-regulates the TGF-beta signaling pathway by promoting dephosphorylation of TGFB1 by PP1. May promote apoptosis by inducing TP53 phosphorylation on 'Ser-15'.1 Publication

GO - Molecular functioni

  • protein phosphatase 1 binding Source: RGD

GO - Biological processi

  • aging Source: RGD
  • apoptotic process Source: UniProtKB-KW
  • cellular response to antibiotic Source: RGD
  • cellular response to calcium ion starvation Source: RGD
  • cellular response to carbohydrate stimulus Source: RGD
  • cellular response to cordycepin Source: RGD
  • cellular response to desipramine Source: RGD
  • cellular response to drug Source: RGD
  • cellular response to gold(3+) Source: RGD
  • cellular response to methamphetamine hydrochloride Source: RGD
  • cellular response to methyl methanesulfonate Source: RGD
  • cellular response to organic cyclic compound Source: RGD
  • cellular response to oxygen-glucose deprivation Source: RGD
  • cellular response to UV Source: RGD
  • endoplasmic reticulum unfolded protein response Source: RGD
  • negative regulation of cell death Source: RGD
  • negative regulation of cell proliferation Source: RGD
  • negative regulation of peptidyl-threonine phosphorylation Source: RGD
  • negative regulation of protein processing Source: RGD
  • positive regulation of apoptotic process Source: RGD
  • positive regulation of protein phosphorylation Source: RGD
  • regulation of angiogenesis Source: RGD
  • regulation of translation Source: UniProtKB-KW
  • response to cocaine Source: RGD
  • response to endoplasmic reticulum stress Source: RGD
  • response to environmental enrichment Source: RGD
  • response to immobilization stress Source: RGD
  • response to organic cyclic compound Source: RGD
Complete GO annotation...

Keywords - Biological processi

Apoptosis, Stress response, Translation regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 1 regulatory subunit 15A
Alternative name(s):
Growth arrest and DNA damage-inducible protein GADD34
Myeloid differentiation primary response protein MyD116 homolog
Progression elevated gene 3 protein
Short name:
PEG-3
Gene namesi
Name:Ppp1r15a
Synonyms:Gadd34, Myd116, Peg3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621526. Ppp1r15a.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2121CytoplasmicBy similarityAdd
BLAST
Intramembranei22 – 3918HelicalBy similarityAdd
BLAST
Topological domaini40 – 578539CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  • cytoplasm Source: RGD
  • cytosol Source: RGD
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • mitochondrial outer membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 578578Protein phosphatase 1 regulatory subunit 15APRO_0000320519Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei236 – 2361PhosphotyrosineBy similarity
Modified residuei365 – 3651PhosphotyrosineBy similarity
Modified residuei419 – 4191PhosphotyrosineBy similarity

Post-translational modificationi

Phosphorylated at multiple Ser/Thr residues. Phosphorylated on tyrosine by LYN; which impairs its antiproliferative activity. Phosphorylation at Tyr-236 enhances proteasomal degradation, this position is dephosphorylated by PTPN2.By similarity
Polyubiquitinated. Exhibits a rapid proteasomal degradation with a half-life under 1 hour, ubiquitination depends on endoplasmic reticulum association.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ6IN02.
PRIDEiQ6IN02.

Expressioni

Inductioni

By lipopolysaccharide.1 Publication

Gene expression databases

BgeeiENSRNOG00000020938.

Interactioni

Subunit structurei

Interacts with PCNA. Interacts with LYN and KMT2A/MLL1. Interacts with PP1, PPP1R1A and SMARCB1. Interacts with SMAD7. Interacts with BAG1 (By similarity).By similarity

GO - Molecular functioni

  • protein phosphatase 1 binding Source: RGD

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000048451.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati279 – 318401Add
BLAST
Repeati319 – 357392Add
BLAST
Repeati358 – 375183Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 165165Required for localization in the endoplasmic reticulumBy similarityAdd
BLAST
Regioni279 – 375973 X approximate repeatsAdd
BLAST
Regioni319 – 41799Interaction with SMAD7By similarityAdd
BLAST
Regioni363 – 462100Interaction with KMT2A/MLL1By similarityAdd
BLAST
Regioni443 – 49048Interaction with SMARCB1By similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi294 – 2974Poly-Glu
Compositional biasi534 – 5374Poly-Ser

Sequence similaritiesi

Belongs to the PPP1R15 family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410ITE6. Eukaryota.
ENOG410Y1YJ. LUCA.
HOGENOMiHOG000060154.
HOVERGENiHBG052542.
InParanoidiQ6IN02.
KOiK14019.
PhylomeDBiQ6IN02.
TreeFamiTF105547.

Family and domain databases

InterProiIPR019523. Prot_Pase1_reg-su15A/B_C.
[Graphical view]
PfamiPF10488. PP1c_bdg. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6IN02-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPSPRPQHV LHWKEAHSFY LLSPLMGFLS RAWSRLRGPE VSEAWLAETV
60 70 80 90 100
AGANQIEADA LLTPPPVSEN HLPLRETEGN GTPEWSKAAQ RLCLDVEAQS
110 120 130 140 150
SPPKTWGLSD IDEHNGKPGQ DGLREQEVEH TAGLPTLQPL HLQGADKKVG
160 170 180 190 200
EVVAREEGVS ELAYPTSHWE GGPAEDEEDT ETVKKAHQAS AASIAPGYKP
210 220 230 240 250
STSVYCPGEA EHRATEEKGT DNKAEPSGSH SRVWEYHTRE RPKQEGETKP
260 270 280 290 300
EQHRAGQSHP CQNAEAEEGG PETSVCSGSA FLKAWVYRPG EDTEEEEDSD
310 320 330 340 350
LDSAEEDTAH TCTTPHTSAF LKAWVYRPGE DTEEEDDGDW DSAEEDASQS
360 370 380 390 400
CTTPHTSAFL KAWVYRPGED TEEEDDSENV APVDSETVDS CQSTQHCLPV
410 420 430 440 450
EKTKGCGEAE PPPFQVAFYL PGQKPAPPWA APKLPLRLQK RLRSFKAPAR
460 470 480 490 500
NQDPEIPLKG RKVHFSEKVT VHFLAVWAGP AQAARRGPWE QFARDRSRFA
510 520 530 540 550
RRIAQAEEQL GPYLTPAFRA RAWTRLRNLP LPLSSSSLPL PEPCSSTEAT
560 570
PLSQDVTTPS PLPSEIPPPS LDLGGRRG
Length:578
Mass (Da):63,570
Last modified:February 26, 2008 - v2
Checksum:i923EC49921C0BC61
GO

Sequence cautioni

The sequence AAC24980 differs from that shown. Reason: Frameshift at positions 416, 504 and 577. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti347 – 3482AS → TA in AAH72513 (PubMed:15489334).Curated
Sequence conflicti453 – 4531D → G in AAC24980 (PubMed:9256446).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF020618 mRNA. Translation: AAC24980.1. Frameshift.
AY128642 Genomic DNA. Translation: AAM77795.1.
BC072513 mRNA. Translation: AAH72513.1.
RefSeqiNP_598230.2. NM_133546.3.
UniGeneiRn.2232.

Genome annotation databases

GeneIDi171071.
KEGGirno:171071.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF020618 mRNA. Translation: AAC24980.1. Frameshift.
AY128642 Genomic DNA. Translation: AAM77795.1.
BC072513 mRNA. Translation: AAH72513.1.
RefSeqiNP_598230.2. NM_133546.3.
UniGeneiRn.2232.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000048451.

Proteomic databases

PaxDbiQ6IN02.
PRIDEiQ6IN02.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi171071.
KEGGirno:171071.

Organism-specific databases

CTDi23645.
RGDi621526. Ppp1r15a.

Phylogenomic databases

eggNOGiENOG410ITE6. Eukaryota.
ENOG410Y1YJ. LUCA.
HOGENOMiHOG000060154.
HOVERGENiHBG052542.
InParanoidiQ6IN02.
KOiK14019.
PhylomeDBiQ6IN02.
TreeFamiTF105547.

Miscellaneous databases

PROiQ6IN02.

Gene expression databases

BgeeiENSRNOG00000020938.

Family and domain databases

InterProiIPR019523. Prot_Pase1_reg-su15A/B_C.
[Graphical view]
PfamiPF10488. PP1c_bdg. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPR15A_RAT
AccessioniPrimary (citable) accession number: Q6IN02
Secondary accession number(s): O88344, Q7TQC2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: February 26, 2008
Last modified: September 7, 2016
This is version 72 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

The sequence described initially (PubMed:9256446) corresponds to a mutant, frameshifted form named PEG-3 that frequently arises during cell transformation and does not seem to exist in normal cells. PEG-3 functions as a dominant negative of GADD34-mediated pro-apoptotic pathway and promotes cancer aggressiveness.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.