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Protein

Protein phosphatase 1 regulatory subunit 3B

Gene

Ppp1r3b

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a glycogen-targeting subunit for phosphatase PP1. Facilitates interaction of the PP1 with enzymes of the glycogen metabolism and regulates its activity. Suppresses the rate at which PP1 dephosphorylates (inactivates) glycogen phosphorylase and enhances the rate at which it activates glycogen synthase and therefore limits glycogen breakdown. Its activity is inhibited by PYGL, resulting in inhibition of the glycogen synthase and glycogen phosphorylase phosphatase activities of PP1. Dramatically increases basal and insulin-stimulated glycogen synthesis upon overexpression in hepatocytes.3 Publications

GO - Molecular functioni

  • enzyme binding Source: RGD
  • protein phosphatase regulator activity Source: RGD

GO - Biological processi

  • glycogen metabolic process Source: UniProtKB-KW
  • protein dephosphorylation Source: GOC
  • regulation of catalytic activity Source: GOC
  • regulation of glycogen biosynthetic process Source: RGD
  • regulation of glycogen catabolic process Source: RGD
Complete GO annotation...

Keywords - Biological processi

Carbohydrate metabolism, Glycogen metabolism

Protein family/group databases

CAZyiCBM21. Carbohydrate-Binding Module Family 21.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 1 regulatory subunit 3B
Alternative name(s):
33 kDa glycogen-binding protein
Hepatic glycogen-targeting protein phosphatase 1 regulatory subunit GL
Protein phosphatase 1 regulatory subunit 4
Short name:
PP1 subunit R4
Protein phosphatase 1 subunit GL
Gene namesi
Name:Ppp1r3b
Synonyms:Ppp1r4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 16

Organism-specific databases

RGDi621600. Ppp1r3b.

Subcellular locationi

GO - Cellular componenti

  • glycogen granule Source: RGD
  • intracellular membrane-bounded organelle Source: RGD
  • protein phosphatase type 1 complex Source: RGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 284284Protein phosphatase 1 regulatory subunit 3BPRO_0000324545Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei260 – 2601PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ6IN01.
PRIDEiQ6IN01.

PTM databases

PhosphoSiteiQ6IN01.

Expressioni

Tissue specificityi

Highly expressed in liver. Moderately expressed in kidney, heart, testis, spleen and lung. Weakly expressed in skeletal muscle (at protein level). Expressed predominantly in liver. Expressed moderately in heart. Expressed weakly in lung, kidney, spleen and skeletal muscle.2 Publications

Gene expression databases

GenevisibleiQ6IN01. RN.

Interactioni

Subunit structurei

Interacts with glycogen, PPP1CC catalytic subunit of PP1 and PYGL. Associates with glycogen particles. Forms complexes with debranching enzyme, glycogen phosphorylase, glycogen synthase and phosphorylase kinase which is necessary for its regulation of PP1 activity.3 Publications

GO - Molecular functioni

  • enzyme binding Source: RGD

Protein-protein interaction databases

IntActiQ6IN01. 1 interaction.
STRINGi10116.ENSRNOP00000042713.

Structurei

3D structure databases

ProteinModelPortaliQ6IN01.
SMRiQ6IN01. Positions 109-252.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini124 – 232109CBM21PROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi61 – 644PP1-binding motif

Domaini

The PP1-binding domain is located between residues 59 and 94. The glycogen-binding domain is located between residues 94 and 257. The PYGL-binding site lies in the C-terminal 16 amino acids.

Sequence similaritiesi

Contains 1 CBM21 (carbohydrate binding type-21) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3986. Eukaryota.
ENOG4111FT1. LUCA.
GeneTreeiENSGT00530000062978.
HOGENOMiHOG000026799.
HOVERGENiHBG052744.
InParanoidiQ6IN01.
KOiK07189.
OMAiMVKVFSE.
OrthoDBiEOG7H4DTW.
PhylomeDBiQ6IN01.
TreeFamiTF105537.

Family and domain databases

InterProiIPR005036. CBM21_dom.
IPR017434. Pase-1_reg-su_3B/C/D_met.
[Graphical view]
PfamiPF03370. CBM_21. 1 hit.
[Graphical view]
PIRSFiPIRSF038207. PP1_GT_animal. 1 hit.
PROSITEiPS51159. CBM21. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6IN01-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVDIEYSYS SMAPSLRRER FTFKISPKLN KPLRPCIQLG SKDEAGRMVA
60 70 80 90 100
PTVQEKKVKK RVSFADNQGL ALTMVKVFSE FDDPLDIPFN ITELLDNIVS
110 120 130 140 150
LTTAESESFV LDFPQPSADY LDFRNRLQTN HVCLENCVLK EKAIAGTVKV
160 170 180 190 200
QNLAFEKVVK IRMTFDTWKS FTDFPCQYVK DTYAGSDRDT FSFDISLPEK
210 220 230 240 250
IQSYERMEFA VCYECNGQSY WDSNKGKNYR ITRAELRSTQ GMTEPYNGPD
260 270 280
FGISFDQFGS PRCSFGLFPE WPSYLGYEKL GPYY
Length:284
Mass (Da):32,626
Last modified:July 5, 2004 - v1
Checksum:i8E64443CCE0EFE83
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti162 – 1621R → S in CAA77083 (PubMed:7498521).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y18208 mRNA. Translation: CAA77083.1.
BC072514 mRNA. Translation: AAH72514.1.
PIRiS68216.
RefSeqiNP_620267.2. NM_138912.2.
XP_006253277.1. XM_006253215.2.
XP_006253278.1. XM_006253216.2.
UniGeneiRn.30046.

Genome annotation databases

EnsembliENSRNOT00000051720; ENSRNOP00000042713; ENSRNOG00000011474.
ENSRNOT00000082091; ENSRNOP00000070738; ENSRNOG00000011474.
GeneIDi192280.
KEGGirno:192280.
UCSCiRGD:621600. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y18208 mRNA. Translation: CAA77083.1.
BC072514 mRNA. Translation: AAH72514.1.
PIRiS68216.
RefSeqiNP_620267.2. NM_138912.2.
XP_006253277.1. XM_006253215.2.
XP_006253278.1. XM_006253216.2.
UniGeneiRn.30046.

3D structure databases

ProteinModelPortaliQ6IN01.
SMRiQ6IN01. Positions 109-252.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ6IN01. 1 interaction.
STRINGi10116.ENSRNOP00000042713.

Protein family/group databases

CAZyiCBM21. Carbohydrate-Binding Module Family 21.

PTM databases

PhosphoSiteiQ6IN01.

Proteomic databases

PaxDbiQ6IN01.
PRIDEiQ6IN01.

Protocols and materials databases

DNASUi192280.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000051720; ENSRNOP00000042713; ENSRNOG00000011474.
ENSRNOT00000082091; ENSRNOP00000070738; ENSRNOG00000011474.
GeneIDi192280.
KEGGirno:192280.
UCSCiRGD:621600. rat.

Organism-specific databases

CTDi79660.
RGDi621600. Ppp1r3b.

Phylogenomic databases

eggNOGiKOG3986. Eukaryota.
ENOG4111FT1. LUCA.
GeneTreeiENSGT00530000062978.
HOGENOMiHOG000026799.
HOVERGENiHBG052744.
InParanoidiQ6IN01.
KOiK07189.
OMAiMVKVFSE.
OrthoDBiEOG7H4DTW.
PhylomeDBiQ6IN01.
TreeFamiTF105537.

Miscellaneous databases

NextBioi623002.
PROiQ6IN01.

Gene expression databases

GenevisibleiQ6IN01. RN.

Family and domain databases

InterProiIPR005036. CBM21_dom.
IPR017434. Pase-1_reg-su_3B/C/D_met.
[Graphical view]
PfamiPF03370. CBM_21. 1 hit.
[Graphical view]
PIRSFiPIRSF038207. PP1_GT_animal. 1 hit.
PROSITEiPS51159. CBM21. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Amino acid sequence and expression of the hepatic glycogen-binding (GL)-subunit of protein phosphatase-1."
    Doherty M.J., Moorhead G., Morrice N., Cohen P., Cohen P.T.W.
    FEBS Lett. 375:294-298(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 42-56; 61-98; 150-157; 161-169; 181-199; 201-220; 238-259 AND 280-284, FUNCTION, INTERACTION WITH PYGL, TISSUE SPECIFICITY.
    Strain: Sprague-Dawley.
    Tissue: Liver.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Heart.
  3. "Purification of the hepatic glycogen-associated form of protein phosphatase-1 by microcystin-Sepharose affinity chromatography."
    Moorhead G., MacKintosh C., Morrice N., Cohen P.
    FEBS Lett. 362:101-105(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 61-76 AND 150-157, INTERACTION WITH PPP1CC AND PYGL, ASSOCIATION WITH GLYCOGEN.
  4. "Identification of the separate domains in the hepatic glycogen-targeting subunit of protein phosphatase 1 that interact with phosphorylase a, glycogen and protein phosphatase 1."
    Armstrong C.G., Doherty M.J., Cohen P.T.W.
    Biochem. J. 336:699-704(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GLYCOGEN; PPP1CC AND PYGL.
  5. "Distinctive regulatory and metabolic properties of glycogen-targeting subunits of protein phosphatase-1 (PTG, GL, GM/RGl) expressed in hepatocytes."
    Gasa R., Jensen P.B., Berman H.K., Brady M.J., DePaoli-Roach A.A., Newgard C.B.
    J. Biol. Chem. 275:26396-26403(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Human skeletal muscle expresses a glycogen-targeting subunit of PP1 that is identical to the insulin-sensitive glycogen-targeting subunit G(L) of liver."
    Munro S., Cuthbertson D.J., Cunningham J., Sales M., Cohen P.T.W.
    Diabetes 51:591-598(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  7. "Expression and glycogenic effect of glycogen-targeting protein phosphatase 1 regulatory subunit GL in cultured human muscle."
    Montori-Grau M., Guitart M., Lerin C., Andreu A.L., Newgard C.B., Garcia-Martinez C., Gomez-Foix A.M.
    Biochem. J. 405:107-113(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiPPR3B_RAT
AccessioniPrimary (citable) accession number: Q6IN01
Secondary accession number(s): Q63759
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: July 5, 2004
Last modified: December 9, 2015
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.