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Protein

Protein phosphatase 1 regulatory subunit 3B

Gene

Ppp1r3b

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a glycogen-targeting subunit for phosphatase PP1. Facilitates interaction of the PP1 with enzymes of the glycogen metabolism and regulates its activity. Suppresses the rate at which PP1 dephosphorylates (inactivates) glycogen phosphorylase and enhances the rate at which it activates glycogen synthase and therefore limits glycogen breakdown. Its activity is inhibited by PYGL, resulting in inhibition of the glycogen synthase and glycogen phosphorylase phosphatase activities of PP1. Dramatically increases basal and insulin-stimulated glycogen synthesis upon overexpression in hepatocytes.3 Publications

GO - Molecular functioni

  • enzyme binding Source: RGD
  • protein phosphatase regulator activity Source: RGD

GO - Biological processi

Keywordsi

Biological processCarbohydrate metabolism, Glycogen metabolism

Protein family/group databases

CAZyiCBM21 Carbohydrate-Binding Module Family 21

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 1 regulatory subunit 3B
Alternative name(s):
33 kDa glycogen-binding protein
Hepatic glycogen-targeting protein phosphatase 1 regulatory subunit GL
Protein phosphatase 1 regulatory subunit 4
Short name:
PP1 subunit R4
Protein phosphatase 1 subunit GL
Gene namesi
Name:Ppp1r3b
Synonyms:Ppp1r4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 16

Organism-specific databases

RGDi621600 Ppp1r3b

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003245451 – 284Protein phosphatase 1 regulatory subunit 3BAdd BLAST284

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei260PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ6IN01
PRIDEiQ6IN01

PTM databases

iPTMnetiQ6IN01
PhosphoSitePlusiQ6IN01

Expressioni

Tissue specificityi

Highly expressed in liver. Moderately expressed in kidney, heart, testis, spleen and lung. Weakly expressed in skeletal muscle (at protein level). Expressed predominantly in liver. Expressed moderately in heart. Expressed weakly in lung, kidney, spleen and skeletal muscle.2 Publications

Gene expression databases

BgeeiENSRNOG00000011474
GenevisibleiQ6IN01 RN

Interactioni

Subunit structurei

Interacts with glycogen, PPP1CC catalytic subunit of PP1 and PYGL. Associates with glycogen particles. Forms complexes with debranching enzyme, glycogen phosphorylase, glycogen synthase and phosphorylase kinase which is necessary for its regulation of PP1 activity.3 Publications

GO - Molecular functioni

  • enzyme binding Source: RGD

Protein-protein interaction databases

IntActiQ6IN01, 1 interactor
STRINGi10116.ENSRNOP00000042713

Structurei

3D structure databases

ProteinModelPortaliQ6IN01
SMRiQ6IN01
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini124 – 232CBM21PROSITE-ProRule annotationAdd BLAST109

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi61 – 64PP1-binding motif4

Domaini

The PP1-binding domain is located between residues 59 and 94. The glycogen-binding domain is located between residues 94 and 257. The PYGL-binding site lies in the C-terminal 16 amino acids.

Phylogenomic databases

eggNOGiKOG3986 Eukaryota
ENOG4111FT1 LUCA
GeneTreeiENSGT00530000062978
HOGENOMiHOG000026799
HOVERGENiHBG052744
InParanoidiQ6IN01
KOiK07189
OMAiMVKVFSE
PhylomeDBiQ6IN01
TreeFamiTF105537

Family and domain databases

Gene3Di2.60.40.2440, 1 hit
InterProiView protein in InterPro
IPR005036 CBM21_dom
IPR038175 CBM21_dom_sf
IPR017434 Pase-1_reg-su_3B/C/D_met
IPR030682 PP1_3B
PANTHERiPTHR12307:SF13 PTHR12307:SF13, 1 hit
PfamiView protein in Pfam
PF03370 CBM_21, 1 hit
PIRSFiPIRSF038207 PP1_GT_animal, 1 hit
PROSITEiView protein in PROSITE
PS51159 CBM21, 1 hit

Sequencei

Sequence statusi: Complete.

Q6IN01-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVDIEYSYS SMAPSLRRER FTFKISPKLN KPLRPCIQLG SKDEAGRMVA
60 70 80 90 100
PTVQEKKVKK RVSFADNQGL ALTMVKVFSE FDDPLDIPFN ITELLDNIVS
110 120 130 140 150
LTTAESESFV LDFPQPSADY LDFRNRLQTN HVCLENCVLK EKAIAGTVKV
160 170 180 190 200
QNLAFEKVVK IRMTFDTWKS FTDFPCQYVK DTYAGSDRDT FSFDISLPEK
210 220 230 240 250
IQSYERMEFA VCYECNGQSY WDSNKGKNYR ITRAELRSTQ GMTEPYNGPD
260 270 280
FGISFDQFGS PRCSFGLFPE WPSYLGYEKL GPYY
Length:284
Mass (Da):32,626
Last modified:July 5, 2004 - v1
Checksum:i8E64443CCE0EFE83
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti162R → S in CAA77083 (PubMed:7498521).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y18208 mRNA Translation: CAA77083.1
BC072514 mRNA Translation: AAH72514.1
PIRiS68216
RefSeqiNP_620267.2, NM_138912.2
XP_006253277.1, XM_006253215.2
XP_006253278.1, XM_006253216.3
UniGeneiRn.30046

Genome annotation databases

EnsembliENSRNOT00000051720; ENSRNOP00000042713; ENSRNOG00000011474
ENSRNOT00000082091; ENSRNOP00000070738; ENSRNOG00000011474
GeneIDi192280
KEGGirno:192280
UCSCiRGD:621600 rat

Similar proteinsi

Entry informationi

Entry nameiPPR3B_RAT
AccessioniPrimary (citable) accession number: Q6IN01
Secondary accession number(s): Q63759
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: July 5, 2004
Last modified: March 28, 2018
This is version 89 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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