Protein phosphatase 1 regulatory subunit 3B

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Q6IN01 (PPR3B_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 51. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Protein phosphatase 1 regulatory subunit 3B

Alternative name(s):
33 kDa glycogen-binding protein
Hepatic glycogen-targeting protein phosphatase 1 regulatory subunit GL
Protein phosphatase 1 regulatory subunit 4
Short name=PP1 subunit R4
Protein phosphatase 1 subunit GL

Gene names

Name:	Ppp1r3b
Synonyms:	Ppp1r4

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	284 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as a glycogen-targeting subunit for phosphatase PP1. Facilitates interaction of the PP1 with enzymes of the glycogen metabolism and regulates its activity. Suppresses the rate at which PP1 dephosphorylates (inactivates) glycogen phosphorylase and enhances the rate at which it activates glycogen synthase and therefore limits glycogen breakdown. Its activity is inhibited by PYGL, resulting in inhibition of the glycogen synthase and glycogen phosphorylase phosphatase activities of PP1. Dramatically increases basal and insulin-stimulated glycogen synthesis upon overexpression in hepatocytes. Ref.1 Ref.5 Ref.7
Subunit structure	Interacts with glycogen, PPP1CC catalytic subunit of PP1 and PYGL. Associates with glycogen particles. Forms complexes with debranching enzyme, glycogen phosphorylase, glycogen synthase and phosphorylase kinase which is necessary for its regulation of PP1 activity. Ref.1 Ref.3 Ref.4
Tissue specificity	Highly expressed in liver. Moderately expressed in kidney, heart, testis, spleen and lung. Weakly expressed in skeletal muscle (at protein level). Expressed predominantly in liver. Expressed moderately in heart. Expressed weakly in lung, kidney, spleen and skeletal muscle. Ref.1 Ref.6
Domain	The PP1-binding domain is located between residues 59 and 94. The glycogen-binding domain is located between residues 94 and 257. The PYGL-binding site lies in the C-terminal 16 amino acids.
Sequence similarities	Contains 1 CBM21 (carbohydrate binding type-21) domain.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glycogen metabolism
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	glycogen metabolic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of glycogen biosynthetic process Inferred from direct assay Ref.3. Source: RGD regulation of glycogen catabolic process Inferred from direct assay Ref.3. Source: RGD
Cellular component	glycogen granule Inferred from direct assay Ref.3. Source: RGD microsome Inferred from direct assay. Source: RGD protein phosphatase type 1 complex Inferred from direct assay Ref.3. Source: RGD soluble fraction Inferred from direct assay. Source: RGD
Molecular function	enzyme binding Inferred from direct assay Ref.3. Source: RGD protein phosphatase regulator activity Inferred from direct assay Ref.1. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 284

284

Protein phosphatase 1 regulatory subunit 3B

PRO_0000324545

Regions

Domain

124 – 232

109

CBM21

Motif

61 – 64

PP1-binding motif

Experimental info

Sequence conflict

162

R → S in CAA77083. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

Q6IN01 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 8E64443CCE0EFE83
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FASTA

284

32,626

        10         20         30         40         50         60 
MAVDIEYSYS SMAPSLRRER FTFKISPKLN KPLRPCIQLG SKDEAGRMVA PTVQEKKVKK 

        70         80         90        100        110        120 
RVSFADNQGL ALTMVKVFSE FDDPLDIPFN ITELLDNIVS LTTAESESFV LDFPQPSADY 

       130        140        150        160        170        180 
LDFRNRLQTN HVCLENCVLK EKAIAGTVKV QNLAFEKVVK IRMTFDTWKS FTDFPCQYVK 

       190        200        210        220        230        240 
DTYAGSDRDT FSFDISLPEK IQSYERMEFA VCYECNGQSY WDSNKGKNYR ITRAELRSTQ 

       250        260        270        280 
GMTEPYNGPD FGISFDQFGS PRCSFGLFPE WPSYLGYEKL GPYY

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Amino acid sequence and expression of the hepatic glycogen-binding (GL)-subunit of protein phosphatase-1." Doherty M.J., Moorhead G., Morrice N., Cohen P., Cohen P.T.W. FEBS Lett. 375:294-298(1995) [PubMed: 7498521] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 42-56; 61-98; 150-157; 161-169; 181-199; 201-220; 238-259 AND 280-284, FUNCTION, INTERACTION WITH PYGL, TISSUE SPECIFICITY. Strain: Sprague-Dawley. Tissue: Liver.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Heart.
[3]	"Purification of the hepatic glycogen-associated form of protein phosphatase-1 by microcystin-Sepharose affinity chromatography." Moorhead G., MacKintosh C., Morrice N., Cohen P. FEBS Lett. 362:101-105(1995) [PubMed: 7720853] [Abstract] Cited for: PROTEIN SEQUENCE OF 61-76 AND 150-157, INTERACTION WITH PPP1CC AND PYGL, ASSOCIATION WITH GLYCOGEN.
[4]	"Identification of the separate domains in the hepatic glycogen-targeting subunit of protein phosphatase 1 that interact with phosphorylase a, glycogen and protein phosphatase 1." Armstrong C.G., Doherty M.J., Cohen P.T.W. Biochem. J. 336:699-704(1998) [PubMed: 9841883] [Abstract] Cited for: INTERACTION WITH GLYCOGEN; PPP1CC AND PYGL.
[5]	"Distinctive regulatory and metabolic properties of glycogen-targeting subunits of protein phosphatase-1 (PTG, GL, GM/RGl) expressed in hepatocytes." Gasa R., Jensen P.B., Berman H.K., Brady M.J., DePaoli-Roach A.A., Newgard C.B. J. Biol. Chem. 275:26396-26403(2000) [PubMed: 10862764] [Abstract] Cited for: FUNCTION.
[6]	"Human skeletal muscle expresses a glycogen-targeting subunit of PP1 that is identical to the insulin-sensitive glycogen-targeting subunit G(L) of liver." Munro S., Cuthbertson D.J., Cunningham J., Sales M., Cohen P.T.W. Diabetes 51:591-598(2002) [PubMed: 11872655] [Abstract] Cited for: TISSUE SPECIFICITY.
[7]	"Expression and glycogenic effect of glycogen-targeting protein phosphatase 1 regulatory subunit GL in cultured human muscle." Montori-Grau M., Guitart M., Lerin C., Andreu A.L., Newgard C.B., Garcia-Martinez C., Gomez-Foix A.M. Biochem. J. 405:107-113(2007) [PubMed: 17555403] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y18208 mRNA. Translation: CAA77083.1. BC072514 mRNA. Translation: AAH72514.1.
IPI	IPI00557670.
PIR	S68216.
RefSeq	NP_620267.2. NM_138912.2.
UniGene	Rn.30046.
3D structure databases
ProteinModelPortal	Q6IN01.
SMR	Q6IN01. Positions 109-252.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q6IN01. 1 interaction.
STRING	Q6IN01.
Protein family/group databases
CAZy	CBM21. Carbohydrate-Binding Module Family 21.
PTM databases
PhosphoSite	Q6IN01.
Proteomic databases
PRIDE	Q6IN01.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000051720; ENSRNOP00000042713; ENSRNOG00000011474.
GeneID	192280.
KEGG	rno:192280.
UCSC	NM_138912. rat.
Organism-specific databases
CTD	79660.
RGD	621600. Ppp1r3b.
Phylogenomic databases
eggNOG	roNOG15596.
GeneTree	ENSGT00530000062978.
HOVERGEN	HBG052744.
InParanoid	Q6IN01.
OrthoDB	EOG47PX72.
Gene expression databases
ArrayExpress	Q6IN01.
Genevestigator	Q6IN01.
Family and domain databases
InterPro	IPR005036. CBM_21. IPR017434. Pase-1_Glycogen_target-su_met. [Graphical view]
KO	K07189.
Pfam	PF03370. CBM_21. 1 hit. [Graphical view]
PIRSF	PIRSF038207. PP1_GT_animal. 1 hit.
PROSITE	PS51159. CBM21. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	623002.

Entry information

Entry name

PPR3B_RAT

Accession

Primary (citable) accession number: Q6IN01
Secondary accession number(s): Q63759

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	July 5, 2004
Last modified:	December 14, 2011
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents