Reviewed, UniProtKB/Swiss-Prot Q6IN01 (PPR3B_RAT)

Names and origin

Protein names

Recommended name:
    Protein phosphatase 1 regulatory subunit 3B
Alternative name(s):
    Protein phosphatase 1 regulatory subunit 4
      Short name=R4
    Protein phosphatase 1 subunit GL
    Hepatic glycogen-targeting protein phosphatase 1 regulatory subunit GL
    33 kDa glycogen-binding protein

Gene names

Name:	Ppp1r3b
Synonyms:	Ppp1r4

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	284 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Acts as a glycogen-targeting subunit for phosphatase PP1. Facilitates interaction of the PP1 with enzymes of the glycogen metabolism and regulates its activity. Suppresses the rate at which PP1 dephosphorylates (inactivates) glycogen phosphorylase and enhances the rate at which it activates glycogen synthase and therefore limits glycogen breakdown. Its activity is inhibited by PYGL, resulting in inhibition of the glycogen synthase and glycogen phosphorylase phosphatase activities of PP1. Dramatically increases basal and insulin-stimulated glycogen synthesis upon overexpression in hepatocytes. Ref.1 Ref.5 Ref.7
Subunit structure	Interacts with glycogen, PPP1CC catalytic subunit of PP1 and PYGL. Associates with glycogen particles. Forms complexes with debranching enzyme, glycogen phosphorylase, glycogen synthase and phosphorylase kinase which is necessary for its regulation of PP1 activity. Ref.1 Ref.3 Ref.4
Tissue specificity	Highly expressed in liver. Moderately expressed in kidney, heart, testis, spleen and lung. Weakly expressed in skeletal muscle (at protein level). Expressed predominantly in liver. Expressed moderately in heart. Expressed weakly in lung, kidney, spleen and skeletal muscle. Ref.1 Ref.6
Domain	The PP1-binding domain is located between residues 59 and 94. The glycogen-binding domain is located between residues 94 and 257. The PYGL-binding site lies in the C-terminal 16 amino acids.
Sequence similarities	Contains 1 CBM21 (carbohydrate binding type-21) domain.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glycogen metabolism
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	glycogen metabolic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of glycogen biosynthetic process Ref.3 Inferred from direct assay. Source: RGD regulation of glycogen catabolic process Ref.3 Inferred from direct assay. Source: RGD
Cellular component	glycogen granule Ref.3 Inferred from direct assay. Source: RGD microsome Inferred from direct assay. Source: RGD protein phosphatase type 1 complex Ref.1 Ref.3 Inferred from direct assay. Source: RGD soluble fraction Inferred from direct assay. Source: RGD
Molecular function	enzyme binding Ref.3 Inferred from direct assay. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 284	284	Protein phosphatase 1 regulatory subunit 3B		PRO_0000324545
Regions
	Domain	124 – 232	109	CBM21
Experimental info
	Sequence conflict	162	1	R → S in CAA77083. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q6IN01-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 8E64443CCE0EFE83	FASTA	284	32,626
	10 20 30 40 50 60 MAVDIEYSYS SMAPSLRRER FTFKISPKLN KPLRPCIQLG SKDEAGRMVA PTVQEKKVKK 70 80 90 100 110 120 RVSFADNQGL ALTMVKVFSE FDDPLDIPFN ITELLDNIVS LTTAESESFV LDFPQPSADY 130 140 150 160 170 180 LDFRNRLQTN HVCLENCVLK EKAIAGTVKV QNLAFEKVVK IRMTFDTWKS FTDFPCQYVK 190 200 210 220 230 240 DTYAGSDRDT FSFDISLPEK IQSYERMEFA VCYECNGQSY WDSNKGKNYR ITRAELRSTQ 250 260 270 280 GMTEPYNGPD FGISFDQFGS PRCSFGLFPE WPSYLGYEKL GPYY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Amino acid sequence and expression of the hepatic glycogen-binding (GL)-subunit of protein phosphatase-1." Doherty M.J., Moorhead G., Morrice N., Cohen P., Cohen P.T.W. FEBS Lett. 375:294-298(1995) [PubMed: 7498521] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 42-56; 61-98; 150-157; 161-169; 181-199; 201-220; 238-259 AND 280-284, FUNCTION, INTERACTION WITH PYGL, TISSUE SPECIFICITY. Strain: Sprague-Dawley. Tissue: Liver.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Heart.
[3]	"Purification of the hepatic glycogen-associated form of protein phosphatase-1 by microcystin-Sepharose affinity chromatography." Moorhead G., MacKintosh C., Morrice N., Cohen P. FEBS Lett. 362:101-105(1995) [PubMed: 7720853] [Abstract] Cited for: PROTEIN SEQUENCE OF 61-76 AND 150-157, INTERACTION WITH PPP1CC AND PYGL, ASSOCIATION WITH GLYCOGEN.
[4]	"Identification of the separate domains in the hepatic glycogen-targeting subunit of protein phosphatase 1 that interact with phosphorylase a, glycogen and protein phosphatase 1." Armstrong C.G., Doherty M.J., Cohen P.T.W. Biochem. J. 336:699-704(1998) [PubMed: 9841883] [Abstract] Cited for: INTERACTION WITH GLYCOGEN; PPP1CC AND PYGL.
[5]	"Distinctive regulatory and metabolic properties of glycogen-targeting subunits of protein phosphatase-1 (PTG, GL, GM/RGl) expressed in hepatocytes." Gasa R., Jensen P.B., Berman H.K., Brady M.J., DePaoli-Roach A.A., Newgard C.B. J. Biol. Chem. 275:26396-26403(2000) [PubMed: 10862764] [Abstract] Cited for: FUNCTION.
[6]	"Human skeletal muscle expresses a glycogen-targeting subunit of PP1 that is identical to the insulin-sensitive glycogen-targeting subunit G(L) of liver." Munro S., Cuthbertson D.J., Cunningham J., Sales M., Cohen P.T.W. Diabetes 51:591-598(2002) [PubMed: 11872655] [Abstract] Cited for: TISSUE SPECIFICITY.
[7]	"Expression and glycogenic effect of glycogen-targeting protein phosphatase 1 regulatory subunit GL in cultured human muscle." Montori-Grau M., Guitart M., Lerin C., Andreu A.L., Newgard C.B., Garcia-Martinez C., Gomez-Foix A.M. Biochem. J. 405:107-113(2007) [PubMed: 17555403] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y18208 mRNA. Translation: CAA77083.1. BC072514 mRNA. Translation: AAH72514.1.
IPI	IPI00557670.
PIR	S68216.
RefSeq	NP_620267.2.
UniGene	Rn.30046
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	CBM21. Carbohydrate-Binding Module Family 21.
Genome annotation databases
Ensembl	ENSRNOG00000011474. Rattus norvegicus. [Contig view]
GeneID	192280.
KEGG	rno:192280.
Organism-specific databases
RGD	621600. Ppp1r3b.
Phylogenomic databases
HOVERGEN	Q6IN01.
Gene expression databases
ArrayExpress	Q6IN01.
Family and domain databases
InterPro	IPR005036. CBM_21. IPR017434. Pase-1_Glycogen_target-su_met. [Graphical view]
Pfam	PF03370. CBM_21. 1 hit. [Graphical view]
PIRSF	PIRSF038207. PP1_GT_animal. 1 hit.
PROSITE	PS51159. CBM21. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	623002.

Entry information

Entry name

PPR3B_RAT

Accession

Primary (citable) accession number: Q6IN01
Secondary accession number(s): Q63759

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	July 5, 2004
Last modified:	June 16, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)