Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Nuclear factor interleukin-3-regulated protein

Gene

Nfil3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Acts as a transcriptional regulator that recognizes and binds to the sequence 5'-[GA]TTA[CT]GTAA[CT]-3', a sequence present in many cellular and viral promoters. Represses transcription from promoters with activating transcription factor (ATF) sites. Represses promoter activity in osteoblasts. Represses transcriptional activity of PER1. Represses transcriptional activity of PER2 via the B-site on the promoter. Activates transcription from the interleukin-3 promoter in T-cells. Competes for the same consensus-binding site with PAR DNA-binding factors (DBP, HLF and TEF). Component of the circadian clock that acts as a negative regulator for the circadian expression of PER2 oscillation in the cell-autonomous core clock. Protects pro-B cells from programmed cell death (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Biological rhythms, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear factor interleukin-3-regulated protein
Alternative name(s):
E4 promoter-binding protein 4
Gene namesi
Name:Nfil3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 17

Organism-specific databases

RGDi620972. Nfil3.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002926691 – 462Nuclear factor interleukin-3-regulated proteinAdd BLAST462

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki219Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity
Cross-linki219Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei301PhosphoserineBy similarity1
Cross-linki337Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei353PhosphoserineBy similarity1
Cross-linki406Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki434Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki448Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ6IMZ0.
PRIDEiQ6IMZ0.

PTM databases

iPTMnetiQ6IMZ0.
PhosphoSitePlusiQ6IMZ0.

Expressioni

Inductioni

Up-regulated by dexamethasone and thapsigargin.1 Publication

Gene expression databases

BgeeiENSRNOG00000011668.
GenevisibleiQ6IMZ0. RN.

Interactioni

Subunit structurei

Homodimer. Binds DNA as a dimer. Interacts with CRY2, DR1 and PER2 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000015525.

Structurei

3D structure databases

ProteinModelPortaliQ6IMZ0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini73 – 136bZIPPROSITE-ProRule annotationAdd BLAST64

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni79 – 95Basic motifPROSITE-ProRule annotationAdd BLAST17
Regioni99 – 106Leucine-zipperPROSITE-ProRule annotation8

Sequence similaritiesi

Belongs to the bZIP family. NFIL3 subfamily.Curated
Contains 1 bZIP (basic-leucine zipper) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410ISXX. Eukaryota.
ENOG410Z2VS. LUCA.
GeneTreeiENSGT00530000064087.
HOGENOMiHOG000059596.
HOVERGENiHBG105717.
InParanoidiQ6IMZ0.
KOiK09059.
OMAiAREPRDD.
OrthoDBiEOG091G06SA.
PhylomeDBiQ6IMZ0.
TreeFamiTF328374.

Family and domain databases

InterProiIPR004827. bZIP.
IPR016743. NFIL3/E4BP4.
IPR010533. Vert_IL3-reg_TF.
[Graphical view]
PANTHERiPTHR15284:SF1. PTHR15284:SF1. 1 hit.
PfamiPF07716. bZIP_2. 1 hit.
PF06529. Vert_IL3-reg_TF. 1 hit.
[Graphical view]
PIRSFiPIRSF019029. bZIP_E4BP4. 1 hit.
SMARTiSM00338. BRLZ. 1 hit.
[Graphical view]
PROSITEiPS50217. BZIP. 1 hit.
PS00036. BZIP_BASIC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6IMZ0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQLRKMQAIK KEPASLDPTG SSDKMLLLNS ALAEVAEDLA SGEDLLLNEG
60 70 80 90 100
SMGKNKSSAC RRKREFIPDE KKDAMYWEKR RKNNEAAKRS REKRRLNDLV
110 120 130 140 150
LENKLIALGE ENATLKAELL SLKLKFGLIS STVYAQEIQK LSNSTAVYFQ
160 170 180 190 200
DYQTSKAAVS SYVDEHEPAM VAGSCISVIK HSPQSSLSDV SEVSSVEHTQ
210 220 230 240 250
ESPAQGGCRS PENKFPVIKQ EPVELESFAR ESREERGAYS ASIYQSYMGS
260 270 280 290 300
SFSTYSHSPP LLQVHGSTSN SPRTSEADEG VVGKSSDGED EQQVPKGPIH
310 320 330 340 350
SPVELQRVHA TVVKVPEVNP SALPHKLRIK AKAMQVKVEA LDSEFEGMQK
360 370 380 390 400
LSSPADALAK RHFDLEKHGA SGTAHSSLPP FSVQVTNIQD WSLRSEHWHH
410 420 430 440 450
KELGGKTQGT FKTGVVEVKD SGYKVSEAEN LYLKQGVANL SAEVVSLKRF
460
IATQPISASD SR
Length:462
Mass (Da):50,781
Last modified:July 5, 2004 - v1
Checksum:i1D7365C2F4B017EF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti45L → P in AAF86615 (PubMed:11262393).Curated1
Sequence conflicti144S → P in AAF86615 (PubMed:11262393).Curated1
Sequence conflicti270N → S in AAF86615 (PubMed:11262393).Curated1
Sequence conflicti355A → V in AAF86615 (PubMed:11262393).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY004663 mRNA. Translation: AAF86615.1.
BC072527 mRNA. Translation: AAH72527.1.
RefSeqiNP_446179.2. NM_053727.2.
XP_006253729.1. XM_006253667.3.
XP_006253731.1. XM_006253669.2.
XP_006253732.1. XM_006253670.3.
UniGeneiRn.54147.

Genome annotation databases

EnsembliENSRNOT00000015525; ENSRNOP00000015525; ENSRNOG00000011668.
GeneIDi114519.
KEGGirno:114519.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY004663 mRNA. Translation: AAF86615.1.
BC072527 mRNA. Translation: AAH72527.1.
RefSeqiNP_446179.2. NM_053727.2.
XP_006253729.1. XM_006253667.3.
XP_006253731.1. XM_006253669.2.
XP_006253732.1. XM_006253670.3.
UniGeneiRn.54147.

3D structure databases

ProteinModelPortaliQ6IMZ0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000015525.

PTM databases

iPTMnetiQ6IMZ0.
PhosphoSitePlusiQ6IMZ0.

Proteomic databases

PaxDbiQ6IMZ0.
PRIDEiQ6IMZ0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000015525; ENSRNOP00000015525; ENSRNOG00000011668.
GeneIDi114519.
KEGGirno:114519.

Organism-specific databases

CTDi4783.
RGDi620972. Nfil3.

Phylogenomic databases

eggNOGiENOG410ISXX. Eukaryota.
ENOG410Z2VS. LUCA.
GeneTreeiENSGT00530000064087.
HOGENOMiHOG000059596.
HOVERGENiHBG105717.
InParanoidiQ6IMZ0.
KOiK09059.
OMAiAREPRDD.
OrthoDBiEOG091G06SA.
PhylomeDBiQ6IMZ0.
TreeFamiTF328374.

Miscellaneous databases

PROiQ6IMZ0.

Gene expression databases

BgeeiENSRNOG00000011668.
GenevisibleiQ6IMZ0. RN.

Family and domain databases

InterProiIPR004827. bZIP.
IPR016743. NFIL3/E4BP4.
IPR010533. Vert_IL3-reg_TF.
[Graphical view]
PANTHERiPTHR15284:SF1. PTHR15284:SF1. 1 hit.
PfamiPF07716. bZIP_2. 1 hit.
PF06529. Vert_IL3-reg_TF. 1 hit.
[Graphical view]
PIRSFiPIRSF019029. bZIP_E4BP4. 1 hit.
SMARTiSM00338. BRLZ. 1 hit.
[Graphical view]
PROSITEiPS50217. BZIP. 1 hit.
PS00036. BZIP_BASIC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNFIL3_RAT
AccessioniPrimary (citable) accession number: Q6IMZ0
Secondary accession number(s): Q923M2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 26, 2007
Last sequence update: July 5, 2004
Last modified: November 2, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.