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Protein

Aldose reductase A

Gene

alrA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies (By similarity). Probably affects several metabolic pathways in addition to converting glucose to sorbitol. Affects group size.By similarity

Catalytic activityi

Alditol + NAD(P)+ = aldose + NAD(P)H.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei49 – 491Proton donorBy similarity
Binding sitei112 – 1121SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 1910NADPSequence Analysis
Nucleotide bindingi207 – 25953NADPBy similarityAdd
BLAST

GO - Molecular functioni

  1. alditol:NADP+ 1-oxidoreductase activity Source: dictyBase

GO - Biological processi

  1. cell motility Source: dictyBase
  2. glucose homeostasis Source: dictyBase
  3. negative regulation of aggregate size involved in sorocarp development Source: dictyBase
  4. response to bacterium Source: dictyBase
  5. response to oxidative stress Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Aldose reductase A (EC:1.1.1.21)
Short name:
ARA
Alternative name(s):
Aldehyde reductase A
Gene namesi
Name:alrA
ORF Names:DDB_G0293850
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195 Componentsi: Chromosome 6, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0293850. alrA.

Subcellular locationi

  1. Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytosol Source: dictyBase
  2. phagocytic vesicle Source: dictyBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Cells show a decrease in the ability to reduce both glyceraldehyde and glucose in an NADPH-coupled reaction and a decrease in glucose levels. They grow normally but form long unbroken streams and huge groups. They show normal adhesion but a reduced motility. They secrete low levels of countin and CF50, two component of the counting factor (CF) but are responsive to CF and partially responsive to recombinant countin and CF50.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 297297Aldose reductase APRO_0000327645Add
BLAST

Proteomic databases

PRIDEiQ6IMN8.

Expressioni

Developmental stagei

Present in vegetative cells; the levels decrease slightly upon starvation and remain constant until 10 hours of development and then decline. At 25 hours there is very little detectable protein.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB_0215363.

Structurei

3D structure databases

ProteinModelPortaliQ6IMN8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aldo/keto reductase family.Curated

Phylogenomic databases

eggNOGiCOG0656.
InParanoidiQ6IMN8.
OMAiVENYRIR.
PhylomeDBiQ6IMN8.

Family and domain databases

Gene3Di3.20.20.100. 1 hit.
InterProiIPR001395. Aldo/ket_red.
IPR018170. Aldo/ket_reductase_CS.
IPR020471. Aldo/keto_reductase_subgr.
IPR023210. NADP_OxRdtase_dom.
[Graphical view]
PANTHERiPTHR11732. PTHR11732. 1 hit.
PfamiPF00248. Aldo_ket_red. 1 hit.
[Graphical view]
PIRSFiPIRSF000097. AKR. 1 hit.
PRINTSiPR00069. ALDKETRDTASE.
SUPFAMiSSF51430. SSF51430. 1 hit.
PROSITEiPS00798. ALDOKETO_REDUCTASE_1. 1 hit.
PS00062. ALDOKETO_REDUCTASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6IMN8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPSFKLSSG HKIPLVGFGT WKAETTLVGK AVEVALDAGY RHIDCAAVYL
60 70 80 90 100
NEKEVGEAFT KKFTTEATVK REDVFITSKL WNTFHKKEHV RPALERTLSD
110 120 130 140 150
LGLQYLDLYL VHWPVAFEYT SNDIQTSGST QEFVSIRETW EEMEKLVDAG
160 170 180 190 200
LVKSIGLSNF NVQGLMEVLS YARIKPAANQ VELHPFLSQP ELKKFCDKHN
210 220 230 240 250
IHLTAYSPLG NGAFVDNEEV GAIAKKYNKT IPNVLCKWAI QKNFSVIPKS
260 270 280 290
STPSRVAENF DLFNFEIEEA DMLFLDKMDK NLRTCDPAKF WGVPLFN
Length:297
Mass (Da):33,649
Last modified:July 5, 2004 - v1
Checksum:i4716A0B09F61D861
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000223 Genomic DNA. Translation: EAL60496.1.
BK001032 mRNA. Translation: DAA01127.1.
RefSeqiXP_628918.1. XM_628916.1.

Genome annotation databases

EnsemblProtistsiDDB0215363; DDB0215363; DDB_G0293850.
GeneIDi8629459.
KEGGiddi:DDB_G0293850.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000223 Genomic DNA. Translation: EAL60496.1.
BK001032 mRNA. Translation: DAA01127.1.
RefSeqiXP_628918.1. XM_628916.1.

3D structure databases

ProteinModelPortaliQ6IMN8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB_0215363.

Proteomic databases

PRIDEiQ6IMN8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiDDB0215363; DDB0215363; DDB_G0293850.
GeneIDi8629459.
KEGGiddi:DDB_G0293850.

Organism-specific databases

dictyBaseiDDB_G0293850. alrA.

Phylogenomic databases

eggNOGiCOG0656.
InParanoidiQ6IMN8.
OMAiVENYRIR.
PhylomeDBiQ6IMN8.

Miscellaneous databases

PROiQ6IMN8.

Family and domain databases

Gene3Di3.20.20.100. 1 hit.
InterProiIPR001395. Aldo/ket_red.
IPR018170. Aldo/ket_reductase_CS.
IPR020471. Aldo/keto_reductase_subgr.
IPR023210. NADP_OxRdtase_dom.
[Graphical view]
PANTHERiPTHR11732. PTHR11732. 1 hit.
PfamiPF00248. Aldo_ket_red. 1 hit.
[Graphical view]
PIRSFiPIRSF000097. AKR. 1 hit.
PRINTSiPR00069. ALDKETRDTASE.
SUPFAMiSSF51430. SSF51430. 1 hit.
PROSITEiPS00798. ALDOKETO_REDUCTASE_1. 1 hit.
PS00062. ALDOKETO_REDUCTASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  2. "Disruption of aldehyde reductase increases group size in dictyostelium."
    Ehrenman K., Yang G., Hong W.-P., Gao T., Jang W., Brock D.A., Hatton R.D., Shoemaker J.D., Gomer R.H.
    J. Biol. Chem. 279:837-847(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
    Strain: AX4.

Entry informationi

Entry nameiALRA_DICDI
AccessioniPrimary (citable) accession number: Q6IMN8
Secondary accession number(s): Q54B70
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: July 5, 2004
Last modified: March 4, 2015
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.