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Q6IMN8

- ALRA_DICDI

UniProt

Q6IMN8 - ALRA_DICDI

Protein

Aldose reductase A

Gene

alrA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 67 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies By similarity. Probably affects several metabolic pathways in addition to converting glucose to sorbitol. Affects group size.By similarity

    Catalytic activityi

    Alditol + NAD(P)+ = aldose + NAD(P)H.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei49 – 491Proton donorBy similarity
    Binding sitei112 – 1121SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi10 – 1910NADPSequence Analysis
    Nucleotide bindingi207 – 25953NADPBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. alditol:NADP+ 1-oxidoreductase activity Source: dictyBase

    GO - Biological processi

    1. cellular component movement Source: dictyBase
    2. glucose homeostasis Source: dictyBase
    3. negative regulation of aggregate size involved in sorocarp development Source: dictyBase
    4. response to bacterium Source: dictyBase
    5. response to oxidative stress Source: dictyBase

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NADP

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aldose reductase A (EC:1.1.1.21)
    Short name:
    ARA
    Alternative name(s):
    Aldehyde reductase A
    Gene namesi
    Name:alrA
    ORF Names:DDB_G0293850
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 6, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0293850. alrA.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytosol Source: dictyBase
    2. phagocytic vesicle Source: dictyBase

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Disruption phenotypei

    Cells show a decrease in the ability to reduce both glyceraldehyde and glucose in an NADPH-coupled reaction and a decrease in glucose levels. They grow normally but form long unbroken streams and huge groups. They show normal adhesion but a reduced motility. They secrete low levels of countin and CF50, two component of the counting factor (CF) but are responsive to CF and partially responsive to recombinant countin and CF50.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 297297Aldose reductase APRO_0000327645Add
    BLAST

    Proteomic databases

    PRIDEiQ6IMN8.

    Expressioni

    Developmental stagei

    Present in vegetative cells; the levels decrease slightly upon starvation and remain constant until 10 hours of development and then decline. At 25 hours there is very little detectable protein.1 Publication

    Interactioni

    Protein-protein interaction databases

    STRINGi44689.DDB_0215363.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6IMN8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the aldo/keto reductase family.Curated

    Phylogenomic databases

    eggNOGiCOG0656.
    PhylomeDBiQ6IMN8.

    Family and domain databases

    Gene3Di3.20.20.100. 1 hit.
    InterProiIPR001395. Aldo/ket_red.
    IPR018170. Aldo/ket_reductase_CS.
    IPR020471. Aldo/keto_reductase_subgr.
    IPR023210. NADP_OxRdtase_dom.
    [Graphical view]
    PANTHERiPTHR11732. PTHR11732. 1 hit.
    PfamiPF00248. Aldo_ket_red. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000097. AKR. 1 hit.
    PRINTSiPR00069. ALDKETRDTASE.
    SUPFAMiSSF51430. SSF51430. 1 hit.
    PROSITEiPS00798. ALDOKETO_REDUCTASE_1. 1 hit.
    PS00062. ALDOKETO_REDUCTASE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q6IMN8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEPSFKLSSG HKIPLVGFGT WKAETTLVGK AVEVALDAGY RHIDCAAVYL    50
    NEKEVGEAFT KKFTTEATVK REDVFITSKL WNTFHKKEHV RPALERTLSD 100
    LGLQYLDLYL VHWPVAFEYT SNDIQTSGST QEFVSIRETW EEMEKLVDAG 150
    LVKSIGLSNF NVQGLMEVLS YARIKPAANQ VELHPFLSQP ELKKFCDKHN 200
    IHLTAYSPLG NGAFVDNEEV GAIAKKYNKT IPNVLCKWAI QKNFSVIPKS 250
    STPSRVAENF DLFNFEIEEA DMLFLDKMDK NLRTCDPAKF WGVPLFN 297
    Length:297
    Mass (Da):33,649
    Last modified:July 5, 2004 - v1
    Checksum:i4716A0B09F61D861
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000223 Genomic DNA. Translation: EAL60496.1.
    BK001032 mRNA. Translation: DAA01127.1.
    RefSeqiXP_628918.1. XM_628916.1.

    Genome annotation databases

    EnsemblProtistsiDDB0215363; DDB0215363; DDB_G0293850.
    GeneIDi8629459.
    KEGGiddi:DDB_G0293850.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000223 Genomic DNA. Translation: EAL60496.1 .
    BK001032 mRNA. Translation: DAA01127.1 .
    RefSeqi XP_628918.1. XM_628916.1.

    3D structure databases

    ProteinModelPortali Q6IMN8.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDB_0215363.

    Proteomic databases

    PRIDEi Q6IMN8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0215363 ; DDB0215363 ; DDB_G0293850 .
    GeneIDi 8629459.
    KEGGi ddi:DDB_G0293850.

    Organism-specific databases

    dictyBasei DDB_G0293850. alrA.

    Phylogenomic databases

    eggNOGi COG0656.
    PhylomeDBi Q6IMN8.

    Miscellaneous databases

    PROi Q6IMN8.

    Family and domain databases

    Gene3Di 3.20.20.100. 1 hit.
    InterProi IPR001395. Aldo/ket_red.
    IPR018170. Aldo/ket_reductase_CS.
    IPR020471. Aldo/keto_reductase_subgr.
    IPR023210. NADP_OxRdtase_dom.
    [Graphical view ]
    PANTHERi PTHR11732. PTHR11732. 1 hit.
    Pfami PF00248. Aldo_ket_red. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000097. AKR. 1 hit.
    PRINTSi PR00069. ALDKETRDTASE.
    SUPFAMi SSF51430. SSF51430. 1 hit.
    PROSITEi PS00798. ALDOKETO_REDUCTASE_1. 1 hit.
    PS00062. ALDOKETO_REDUCTASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    2. "Disruption of aldehyde reductase increases group size in dictyostelium."
      Ehrenman K., Yang G., Hong W.-P., Gao T., Jang W., Brock D.A., Hatton R.D., Shoemaker J.D., Gomer R.H.
      J. Biol. Chem. 279:837-847(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
      Strain: AX4.

    Entry informationi

    Entry nameiALRA_DICDI
    AccessioniPrimary (citable) accession number: Q6IMN8
    Secondary accession number(s): Q54B70
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 8, 2008
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 67 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3