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Protein

E3 ubiquitin-protein ligase NHLRC1

Gene

Nhlrc1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

E3 ubiquitin-protein ligase. Together with the phosphatase EPM2A/laforin, appears to be involved in the clearance of toxic polyglucosan and protein aggregates via multiple pathways. In complex with EPM2A/laforin and HSP70, suppresses the cellular toxicity of misfolded proteins by promoting their degradation through the ubiquitin-proteasome system (UPS). Ubiquitinates the glycogen-targeting protein phosphatase subunits PPP1R3C/PTG and PPP1R3D in a laforin-dependent manner and targets them for proteasome-dependent degradation, thus decreasing glycogen accumulation. Polyubiquitinates EPM2A/laforin and ubiquitinates AGL and targets them for proteasome-dependent degradation. Also promotes proteasome-independent protein degradation through the macroautophagy pathway (By similarity).By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri23 – 6947RING-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Autophagy, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-RNO-3322077. Glycogen synthesis.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase NHLRC1 (EC:6.3.2.-)
Alternative name(s):
Malin
NHL repeat-containing protein 1
Gene namesi
Name:Nhlrc1
Synonyms:Epm2b
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 17

Organism-specific databases

RGDi735081. Nhlrc1.

Subcellular locationi

  • Endoplasmic reticulum By similarity
  • Nucleus By similarity

  • Note: Localizes at the endoplasmic reticulum and, to a lesser extent, in the nucleus.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 396396E3 ubiquitin-protein ligase NHLRC1PRO_0000055982Add
BLAST

Proteomic databases

PaxDbiQ6IMG5.
PRIDEiQ6IMG5.

Interactioni

Subunit structurei

Interacts with AGL. Interacts (via the NHL repeats) with EPM2A/laforin. Forms a complex with EPM2A/laforin and HSP70 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000030625.

Structurei

3D structure databases

ProteinModelPortaliQ6IMG5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati110 – 15445NHL 1Add
BLAST
Repeati158 – 20144NHL 2Add
BLAST
Repeati202 – 24241NHL 3Add
BLAST
Repeati245 – 29854NHL 4Add
BLAST
Repeati299 – 34749NHL 5Add
BLAST
Repeati348 – 39144NHL 6Add
BLAST

Domaini

The RING domain is essential for ubiquitin E3 ligase activity.By similarity

Sequence similaritiesi

Contains 6 NHL repeats.PROSITE-ProRule annotation
Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri23 – 6947RING-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG410ITF2. Eukaryota.
ENOG410ZMHC. LUCA.
GeneTreeiENSGT00730000111361.
HOGENOMiHOG000113780.
HOVERGENiHBG052617.
InParanoidiQ6IMG5.
KOiK10602.
OMAiPRNLPCG.
OrthoDBiEOG7VX8W6.
PhylomeDBiQ6IMG5.
TreeFamiTF331018.

Family and domain databases

Gene3Di2.120.10.30. 1 hit.
3.30.40.10. 1 hit.
InterProiIPR011042. 6-blade_b-propeller_TolB-like.
IPR013017. NHL_repeat_subgr.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF14634. zf-RING_5. 1 hit.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
[Graphical view]
PROSITEiPS51125. NHL. 6 hits.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6IMG5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGEEAAGVRP ELVREAEVSL LECKVCFERF GHRQQRRPRN LPCGHVVCLA
60 70 80 90 100
CVAALAHPRT LALECPFCRR ACRACDTSDC LPVLHLLELL GSTLHASPAA
110 120 130 140 150
LSAASCAPGA LTCYHAFGGW GTLVNPTGLA LCPKTGRVVV VHDGKRRVKI
160 170 180 190 200
FDSGGGGAHQ FGEKGDAAHD VKYPLDVAVT NDCHVVVTDA GDCSLKVFDF
210 220 230 240 250
FGQIKLVVGK QFSLPWGVEI TPHNGVLVTD AEAGTLHLLE ADFPEGVLRR
260 270 280 290 300
IERLQAHLCN PRGVAVSWLT GAIAVLEHPC ALGTSSGNNT RVKVFNSSMQ
310 320 330 340 350
LIGQVDSFGL NLLFPSKITA SAVTFDHQGN VIVADTSGPA IVCLGKPEEF
360 370 380 390
PALKPMVTHG LSRPVALVFT KENSLLVLDS ASHSIKVFKV MEGNGG
Length:396
Mass (Da):42,089
Last modified:July 5, 2004 - v1
Checksum:iA62522DC97976099
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BK001524 mRNA. Translation: DAA01959.1.
RefSeqiNP_954706.1. NM_199236.1.
UniGeneiRn.103777.

Genome annotation databases

EnsembliENSRNOT00000031656; ENSRNOP00000030625; ENSRNOG00000026286.
GeneIDi364682.
KEGGirno:364682.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BK001524 mRNA. Translation: DAA01959.1.
RefSeqiNP_954706.1. NM_199236.1.
UniGeneiRn.103777.

3D structure databases

ProteinModelPortaliQ6IMG5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000030625.

Proteomic databases

PaxDbiQ6IMG5.
PRIDEiQ6IMG5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000031656; ENSRNOP00000030625; ENSRNOG00000026286.
GeneIDi364682.
KEGGirno:364682.

Organism-specific databases

CTDi378884.
RGDi735081. Nhlrc1.

Phylogenomic databases

eggNOGiENOG410ITF2. Eukaryota.
ENOG410ZMHC. LUCA.
GeneTreeiENSGT00730000111361.
HOGENOMiHOG000113780.
HOVERGENiHBG052617.
InParanoidiQ6IMG5.
KOiK10602.
OMAiPRNLPCG.
OrthoDBiEOG7VX8W6.
PhylomeDBiQ6IMG5.
TreeFamiTF331018.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiR-RNO-3322077. Glycogen synthesis.

Miscellaneous databases

PROiQ6IMG5.

Family and domain databases

Gene3Di2.120.10.30. 1 hit.
3.30.40.10. 1 hit.
InterProiIPR011042. 6-blade_b-propeller_TolB-like.
IPR013017. NHL_repeat_subgr.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF14634. zf-RING_5. 1 hit.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
[Graphical view]
PROSITEiPS51125. NHL. 6 hits.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiNHLC1_RAT
AccessioniPrimary (citable) accession number: Q6IMG5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 5, 2004
Last modified: June 8, 2016
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.